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Cotton fiber development related GhFSN1 gene identification and application

A cotton and genetic technology, applied in the fields of application, genetic engineering, plant genetic improvement, etc., can solve the problem of lack of three types of secondary wall components

Inactive Publication Date: 2016-09-21
HUAZHONG NORMAL UNIV
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AI Technical Summary

Problems solved by technology

Simultaneous suppression of the expression of these two NAC genes by RNAi technology resulted in no fiber secondary wall synthesis in Arabidopsis stems, and the lack of three types of secondary wall components, namely cellulose, xylan and lignin, in the fiber cell wall

Method used

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  • Cotton fiber development related GhFSN1 gene identification and application
  • Cotton fiber development related GhFSN1 gene identification and application
  • Cotton fiber development related GhFSN1 gene identification and application

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Embodiment Construction

[0021] Cloning identification and functional analysis of the full-length sequence of a new cotton NAC family gene GhANC1:

[0022] 1. Isolation and identification of GhFSN1 gene sequence and its cDNA sequence

[0023] More than 10,000 cotton cDNA clones were isolated from the cotton fiber cDNA library for DNA sequencing. Through bioinformatics analysis, a full-length cDNA sequence of a cotton NAC gene was identified, named GhFSN1, the sequence is shown in SEQ.ID.No.1, the full length of the gene is 1270, the molecular weight is 43.62KD, and the isoelectric point (pI) is 6.47. Containing an open reading frame of 1152bp, the gene coding region (ORF) is from the start codon ATG to the stop codon TAA (1-1152bp), encoding a 383 amino acid protein, and its amino acid sequence is shown in SEQ.ID.No.3. According to the identified GhFSN1cDNA sequence, the gene-specific primers were designed, and PCR (polymerase chain reaction) technology was used to amplify and clone the DNA sequence...

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Abstract

The invention relates to cotton fiber development related GhFSN1 gene identification and application. The overall length of a cotton GhFSN1 gene sequence is 2,130 bp and is as shown in SEQ. ID. No. 2. The specificity of mRNA of the gene in a secondary development period of cotton fiber cells is accumulated in quantity, which shows that the gene is a specific gene related to fiber secondary wall development. GhFSN1 protein is positioned in a cell nucleus, and has transcription self-activation activity. The GhFSN 1 gene is overexpressed in cotton to cause thickening of cotton fiber cell secondary walls, the cellulose content is increased, but the lengths of mature fibers are reduced. The GhFSN1 protein can form homodimer or heterodimer which is combined onto a downstream target gene promoter, expression of the promoter is regulated and controlled, and biological synthesis of the cotton fiber cell secondary walls is affected. The GhFSN 1 which is used as a transcriptional activation factor plays a role in development of the cotton fiber cell secondary walls, and quality characters such as strength and lengths of the cotton fiber are regulated and controlled.

Description

Technical field: [0001] The present invention relates to cotton genes. Specifically, a NAC transcription factor gene GhFSN1 ( F iber S secondary cell wall-related N Cloning and functional identification of AC transcription factor). Background technique: [0002] Cotton is an important economic crop in my country, which is closely related to the income of 200 million farmers and the employment of 19 million textile workers in China. With the rapid development of the textile industry and the continuous improvement of people's living standards, the textile industry and its related industries have higher and higher requirements for the quality of cotton fibers. my country's total lint production ranks first in the world, and its per unit yield also ranks first among the five major cotton-producing countries. However, the quality of my country's cotton fibers is poor and cannot be used as raw materials for the production of some high-value-added high-quality textile products....

Claims

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Application Information

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IPC IPC(8): C12N15/29C07K14/415
CPCC07K14/415
Inventor 李学宝张杰黄耿青邹丹鄢静秋
Owner HUAZHONG NORMAL UNIV
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