116 results about "N-acetyl-L-cysteine" patented technology

The present invention is directed to stable protein derivatives, e.g., antibodies, antibody fragments or peptides, with at least one free thiol group coupled to N-acetyl-L-cysteine, N-ethyl-maleimide, or cysteine and the methods of making such derivatives. In addition, stable liquid pharmaceutical formulations comprising such proteins or their derivatives and stable lyophilized pharmaceutical formulations comprising such proteins are provided. The present invention is also directed to a method of making a stable Fab′ fragment of an antibody and a method of controlling vascularization in injured or cancerous tissue comprising applying to the injured tissue one or more doses of the pharmaceutical formulations.

The present invention relates to a nutritional supplement composition, comprising a therapeutically effective amounts of Vitamin C, Vitamin D3, Thiamin, Riboflavin, Niacin, Vitamin B6, Folic acid, Vitamin B12, Pantothenic acid, Calcium, Magnesium, Zinc, Chromium, Sugar, Protein, Acetyl-L-Carnitine, Dimethylaminoethanol complex, Phosphatidylserine complex, L-Glutamine, N-Acetyl-L-Tyrosine, L-Phenylalanine, Taurine, Methionine, Valine, Isoleucine, 5 Hydroxytryptophan, L-Taurine, N-Acetyl-Tyrosine, N-Acetyl-L-Cysteine, Alpha Lipoic Acid, Alpha Glycerylphosphoricholine complex, Bacopa Monnieri extract, Gingko Biloba extract, Passion flower, Lemon Balm, Gotu Kola, Ashwagandha, Choline Bitartrate complex, Panax Ginseng extract, Turmeric, Organic freeze dried fruit juice blends (concord grape, red raspberry, pineapple, cranberry, acai, pomegranate, acerola cherry, bilberry, lingonberry, black currant, aronia, sour cherry, black raspberry), Organic freeze dried greens blends (barley grass, broccoli, beet, carrot, alfalfa, oat), and Protein digestive enzyme blends (Protease 4.5, peptidase, bromelain, protease 6.0, protease 3.0, L planatrum, B bifidum) in a mixture to provide optimal cognitive function.

An herbal composition is adapted to treat hangovers. In one embodiment, the herbal composition comprises potassium, thiamin (vitamin B-1), magnesium, silymarin and N-acetyl-L-cysteine (acetylcysteine), or any pharmaceutically acceptable salts thereof. In a method of treating hangovers, an herbal composition is provided that comprises potassium, thiamin, magnesium, silymarin and N-acetyl-L-cysteine. The herbal composition is placed in the body so as to alleviate or reduce the effects of a hangover.

The components of this invention are chosen because of their complementarity for the prevention or treatment of diseases caused by the herpes simplex virus. L-Lysine favorably increases the physiologic immunomodulation necessary for defense against this virus. Zinc improves and maintains a normal immune response. 2-Deoxy-2-D-glucose and heparin sodium alter the surface interaction between the herpes virus and the cell, preventing fusion and infectivity. N-Acetyl-L-cysteine increases glutathione levels thereby creating a thiol redox barrier to the virus at the cell membrane. Quercetin reduces intraoellular replication of the herpes virus and viral infectivity. Ascorbate, in concert with copper and D-α-tocopherol, provides an antioxidant defense against the herpes virus, which tends to lose latency during period of oxidative, free radical excess. Selenium and quercetin also participate in reducing various oxidative stresses. Together the components of this invention provide the potential for improved resistance to, improved recovery from, and a decreased frequency of recurrence of herpes simplex virus infection.

The invention discloses a biocompatible natural polymer hydrogel and a preparation method thereof. The hydrogel is produced by the following steps: chitosan or glucan or gelatin and 1-Hydroxy benzotriazole are dispersed in the water, and stirred until transparent solution is formed; N-Acetyl-L-Cysteine or 2,3-Dimercaptosuccinic acid is added, then dripped into the water solution prepared with 1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide, the pH is adjusted, stirred for 2-4h; dialyzed with 5mmol / L hydrochloric acid solution containing 2Mu mol / L EDTA under lucifugal condition within 2-4 days, dialyzed with 5mmol / L hydrochloric acid solution containing 1g / L NaCl, and then dialyzed with 1mmol / L hydrochloric acid solution to achieve the desired product. The hydrogel has excellent hydrophilicity, moisture retaining property, permeability and flexibility, the degradation product has no toxic or adverse effect to the body, and can meet the requirements of modern medical science on dressing to the largest extent.

Radiation-oxidative exposure treatment compositions comprise a mixture of micronutrient multivitamin and trace elements, a mixture of antioxidants and chemopreventative agents, and optionally a mixture of fatty acids. Micronutrient multivitamin and trace elements mixtures include vitamins A, Bp, B1, B2, B3, B5, B6, B7, B9, B12, C, D, E and K; inositol; calcium, iodine, magnesium, zinc, selenium, copper, manganese, chromium, molybdenum, potassium, boron and vanadium. Mixtures of non-essential antioxidants and chemopreventative agents include bioflavins, alpha lipoic acid, N-acetyl-L-cysteine (optionally) lutein, lycopene, astaxanthin, plant sterols, isoflavones, garlic extract, which provides allicin; green tea extract, cruciferous vegetable extract, fruit extracts, coenzyme Q-10, and resveratrol. Fatty acid mixtures include eicosapentaenoic acid and docosahexaenoic acid.Methods of treatment of a subject exposed to a radiation source or an oxidative stress with the radiation-oxidative exposure treatment composition include the step of administering to the subject a daily dose of the radiation-oxidative exposure treatment composition such that the life shortening effects induced by the radiation source or the oxidative stress are ameliorated.

The invention discloses a ratiometric fluorescent probe and a preparation thereof, and an application of the ratiometric fluorescent probe in detection of glutathione. The ratiometric fluorescent probe is prepared through the following steps: with green quantum dots as cores, carrying out embedding in silica nanoparticles, then subjecting the surface of the silica nanoparticles to amination modification, and covalently coupling red quantum dots onto the amination modified surface of the silica nanoparticles so as to obtain the ratiometric fluorescent probe, wherein the green quantum dots are cadmium telluride quantum dots modified by N-acetyl-L-cysteine. According to the invention, a St ber method is utilized to prepare quantum dot embedded silica nanoparticles, so the synthetic method issimple and has less time consumption. The probe provided by the invention has low detection limit and can realize specific detection during detection of GSH; meanwhile, the probe has advantages like simple preparation and good stability, reduces the detection limit of glutathione to 45 nM, and provides convenience for rapid visual detection.

The invention belongs to the technical field of fluorescent nano-materials and provides a red fluorescent copper nano-cluster and a preparation method thereof, which have the advantages of simplicityin operation, moderate conditions and the quantum yield of 0.44 percent. A synthesis method of the prepared red fluorescent copper nano-cluster is simple, and interference caused by self fluorescent light of living organisms can be avoided; the red fluorescent copper nano-cluster has high sensitivity on Ag<+> ions and can be used for constructing a sensing system for detecting the Ag<+> ions. At room temperature, N-acetyl-L-cysteine is used as a protection agent and a reduction agent and a copper nitrate solution is used as a matrix to prepare a fluorescent copper nano-cluster solution throughan ultrasonic method. The addition of a common reduction agent, i.e., sodium borohydride, ascorbic acid, a surfactant and the like is avoided; the preparation method is simple to operate and feasible, has moderate conditions and is environmentally friendly; the prepared copper nano-cluster has good water solubility, high quantum yield and low toxicity, and can be used for highly sensitively and highly selectively identifying and detecting the Ag<+> ions; a detection process is simple and rapid and a detection result is accurate.

The invention discloses a tumor tissue 3D culture method suitable for culturing various tumor tissues. The tumor tissue 3D culture method comprises the following steps: 1) dropwise adding hydrogel into a pore plate, transferring cut tumor tissue blocks into the hydrogel, dropwise adding a culture solution, and standing in a CO2 incubator in order to freeze the hydrogel; 2) adding the culture solution into the pore plate for culturing, taking the cultured tumor tissue blocks out and fixing, wherein the culture solution is prepared from an Advanced DMEM / F12 culture solution, 1*GlutaMAX, an epidermis growth factor (EGF), a fibroblast growth factor FGF10, N-acetyl-L-cysteine (NAC), prostaglandin E2 PGE2, Nicotinamide and 50*B27. Moreover, the invention further discloses a tumor tissue 3D culture solution. The method is suitable for a plurality of tumor tissues, can be used for rating the medicinal effects of antitumor drugs more rapidly and accurately, and overcomes the defects in cell 2D culturing and animal experiment rating methods; a more convenient and economical modeling method is provided for clinical medicament researches and new medicament development.

A supplement composition for enhancement of methylation process is provided, which contains vitamin B6 (as pyridoxine HCl), folic acid, vitamin B12 (as cyanocobalamin), betaine HCl, and methylsulfonylmethane; and also contains S-adenosylmethionine. The supplement composition further includes silymarin (from milk thistle seed extract), N-acetyl L-cysteine, and cruciferious blend which includes broccoli (brassica oleracea var. talica), kale (brassica oleracea var. acephala), and radish (raphanus sativus). Further provided is a method of using the supplement composition for enhancement of methylation process.

The invention provides a sputum specimen homogenization method and preparation of a reagent for medical detection of a sputum specimen. The invention further provides a novel sputum digestion solution conforming to clinical detection, and the novel sputum digestion solution is prepared from 0.2% (w / v) of N-acetyl-L-cysteine, 0.1% (w / v) of trypsin, 0.4% (w / v) of triethanolamine, 0.2% (w / v) of potassium chloride, 1.5% (w / v) of potassium dihydrogen phosphate, 0.2% (w / v) of dipotassium hydrogen phosphate and a sterile water solvent.

The present invention relates to a culture medium for culturing a mesenchymal stem cell, and more specifically, to a culture medium composition for culturing a mesenchymal stem cell, comprising a basic culture medium, L-ascorbic acid 2-phosphate, fetal bovine serum, basic fibroblast growth factor (b-FGF), non-essential amino acids (NEAA), insulin, N-acetyl-L-cysteine, calcium chloride and hydrocortisone,and a method for culturing a mesenchymal stem cell by using the same. According to the present invention, it is possible to obtain the number of mesenchymal stem cells necessary for stem cell treatment within a short time and the differentiation of mesenchymal stem cells is improved, and is useful for the treatment of cells using a stem cell.

The subject invention pertains to materials and methods for the prevention and treatment of disease conditions associated with oxidative stress or a compromised reducing environment, including inflammatory bowel diseases such as Crohn's disease and ulcerative colitis. Another aspect of the subject invention concerns compositions formulated for administration as an enema. In one embodiment, a composition suitable for administration as an enema comprises an effective amount of 5-ASA and a steroid such as budesonide or hydrocortisone. The subject invention also concerns compositions formulated for oral administration. In one embodiment, a composition comprises alpha-lipoic acid, and / or N-acetyl-L-cysteine (N-A-C), and / or L-glutamine. The alpha-lipoic acid can be racemic alpha-lipoic acid, R-lipoic acid, or R-dihydro-lipoic acid. Methods of the invention include administration of compounds or compositions of the invention. In one embodiment, compounds or compositions of the invention are rectally instilled in a patient. In another embodiment, compounds or compositions are orally administered. The subject invention also concerns methods for screening for, assessing risk of developing, and / or diagnosing conditions associated with oxidative stress, such as ulcerative colitis and other inflammatory bowel disorders.

The invention discloses an NAC (N-acetyl-L-Cysteine) transcription factor gene GmST2 of soybean holy bean No.9 and a plant expression vector of the GmST2. The invention further discloses application of the gene GmST2 in improvement on the salt resistant / drought tolerance in arabidopsis thaliana and soybean plants. Provided by tests, the salt resistance / drought tolerance of a transgenic plant is greatly improved compared with that of a non transgenic plant and theoretical base and practice foundation for improving the salt resistance / drought tolerance of crops through genetic engineering means are provided. The NAC transcription factor gene GmST2 can be widely applied to cultivation of salt-resisting / drought tolerant plant varieties.

The invention discloses hepatoprotective composition. The hepatoprotective composition is prepared from the following raw materials: a milk thistle extract, a green tea extract, an Acai berry extract, alpha-lipoic acid and N-acetyl-L-cysteine. The invention further discloses a preparation method and an application of the hepatoprotective composition. The hepatoprotective composition combines the milk thistle extract, the green tea extract, the Acai berry extract, the alpha-lipoic acid and the N-acetyl-L-cysteine for use, so that the hepatoprotective composition has a significant hepatoprotective function and can be used for treating and / or preserving acute liver injuries and chronic liver injuries.

The present invention provides cell culture media and methods useful for determining levels of intracellular function of glutathione or cysteine and for providing biochemical analysis of antioxidant function in human lymphocytes.

The invention discloses a production method of N-acetyl-L-cysteine. According to the production method disclosed by the invention, the temperature of acylation reaction is controlled at about 60 DEG C. Under the condition of the temperature, not only is reaction on L-cysteine hydrochloride and acetic anhydride more fully, but also the content of the byproduct N-acetyl-D-cysteine in the product is very low. In addition, according to the preparation method disclosed by the invention, the reaction conditions are strictly controlled, such as concentration of an L-cysteine hydrochloride solution, pH, temperature, pressure of the reaction and the adding manner of acetic anhydride, so that reaction on L-cysteine hydrochloride and acetic anhydride is more fully. Excessive acetic anhydride is not needed for acylation reaction so that the impurity content of the product is reduced and the production cost of the product is lowered.

Disclosed is nucleic acid preserving compositions and methods of manufacturing and using the same. Compositions include a carrier, a chaotropic agent, a buffering agent, a chelating agent, a surfactant, an alcohol, an acid, and a mucolytic agent. Compositions as aqueous solutions can include water as a carrier. Preferred embodiments include water, guanidine thiocyanate, Tris, EDTA, SLS, SDA 3C, HCl, and N-acetyl-L-cysteine. Some embodiments include a colored dye as a visual indicator. Methods of manufacturing include combining the components into a mixture, such as an aqueous solution. Methods of use include providing a biological sample that includes nucleic acid and contacting the biological sample with the composition. Kits include the composition disposed in a portion of a biological sample collection apparatus.

The invention discloses a novel cotton fiber secondary development specific expression gene GhNAC1 full-length sequence, and relates to an important cotton fiber development-associated regulator gene element. The GhNAC1 gene contains three exons and two introns, and is coded with an NAC (N Acetyl L Cysteine) transcription factor. A large quantity of mRNAs (messenger Ribonucleic Acids) of the gene are accumulated specifically at the secondary development stage of the cotton fiber cells, which indicates that the gene is a fiber secondary wall development specific gene. GhNAC1 protein is positioned in a cell nucleolus and has the capability of activating transcription independently, which indicates that the protein serving as a transcription activating factor plays a role in cotton fiber development. GhNAC1 is expressed excessively in arabidopsis, so that leaves crimp upwards, cell walls are thickened, ectopic sedimentation of lignin and the like is detected in foliar epidermic cells, so that the hemicellulose content of foliar cells is greatly increased. As proved by the results, the GhNAC1 can be used for regulating the biosynthesis of cell secondary walls, and plays a key role in the developing process of cotton fiber.

The invention belongs to the technical field of production of nanometer composite materials, and provides gold nanocluster / carbon dot composite fluorescent nanoparticles and a production method and application in tetracycline detection thereof. N-acetyl-L-cysteine and glucose are used as a carbon source and a protective agent, chloroauric acid is used as a gold precursor, and through a one-pot method, a gold nanocluster / carbon dot composite fluorescent nanoparticle solution is produced. The production technology is simple, high in reaction speed and environmentally friendly, the emission wavelength of the yellowish green gold nanocluster / carbon dot composite fluorescent nanoparticles are a long wavelength, thus interference of fluorescence of living bodies can be avoided, and the yellowishgreen gold nanocluster / carbon dot composite fluorescent nanoparticles have good water solubility, a high quantum yield, a long fluorescence lifetime, good photobleaching resistance and good photostability, and shows more excellent optical performance than that of a single material. Only tetracycline can significantly quench fluorescence of the gold nanocluster / carbon dot composite fluorescent nanoparticles, a sensing system with good selectivity and high sensibility is built, and the method can be applied for detecting residual tetracycline medicines in food.

The invention discloses a preparation method of a water-soluble Mu-doped CdxMn1-xTe quantum dot. The preparation method comprises the operating step of: filling NaHTe or KHTe solution which is prepared in advance into a mixed solution of cadmium salts, manganese salts and water-soluble N-acetyl-L-cysteine which is prepared in advance under nitrogen protection so as to obtain a water-soluble Mn-doped CdxMn1-xTe quantum dot with an adjustable fluorescence emission wavelength and a high fluorescence quantum yield. The preparation method is carried out in an aqueous solution, and has the advantages of being easy to obtain the raw materials, simple, convenient and safe to operate, green and environment-friendly, and the like. The prepared water-soluble Mn-doped CdxMn1-xTe quantum dot has the advantages of excellent fluorescence properties, good water solubility, excellent fluorescence property, good biocompatibility, low toxicity and the like, and can be used as a novel fluorescent nanoemter probe to be widely applied to the fields of biochemical analysis and biomedicine.

An antioxidant supplement composition is provided, which contains vitamin E, resveratrol, and alpha-lipoic acid. The supplement composition can further contain N-acetyl-L-cysteine. Further provided is a method of using the antioxidant supplement composition for enhancing an individual's resistance to oxidative damages. The method includes administering the antioxidant supplement composition daily in a dosage contains from 50 to 200 IU of vitamin E, from 5 to 40 mg of resveratrol, from 50 to 400 mg of alpha-lipoic acid. In a higher strength treatment, the daily dosage further includes from 150 to 600 mg of N-acetyl-L-cysteine.

A novel substituted N-acetyl-L-cysteine (NAC) derivative and methods of using this compound for the treatment of diseases and / or conditions, including but not limited to diseases and / or conditions of, or involving, the Central Nervous System (CNS), including schizophrenia adrenoleukodystrophy, mitochondrial diseases (e.g. Leigh syndrome, Alpers' disease, and MELAS), Huntington's disease, trichotillomania, HIV-associated neurocognitive disorder, hypoxic-ischemic encephalopathy, drug craving, and drug addiction.

The invention discloses a visual ratio fluorescent system for detecting a cyanide ion, and a preparation method and an application of the system. The visual ratio fluorescent system comprises an N-acetyl-L-cysteine modified CdTe quantum dot, a carbon dot, a copper ion and a phosphate buffer solution. In the visual ratio fluorescent system, the concentration of the copper ion is 0.7-1.2micromoles / L; the concentration of the N-acetyl-L-cysteine modified CdTe quantum dot is 20nmol / L; in a testing system, a ratio of fluorescent intensity of the carbon dot to fluorescent peak intensity of the N-acetyl-L-cysteine modified CdTe quantum dot quenched by the copper ion is (25-35):1; and a pH (potential of hydrogen) value of the visual ratio fluorescent system is 7-8.8. The visual ratio fluorescent system is simple and convenient to prepare, and cheap, and has good cyanide ion selectivity and sensitivity, a wide linear range and a very low limit of detection.

The invention discloses a growth promoting agent for promoting the growth of a weaned pig and an application. The growth promoting agent consists of N-acetyl-L-cysteine and acetaminophen at a certain ratio, and is prepared according to the following steps: step A, proportionally weighing the N-acetyl-L-cysteine; step B, proportionally weighing the acetaminophen; step C, sequentially adding the weighed N-acetyl-L-cysteine and acetaminophen into a mixer and fully mixing to obtain the growth promoting agent applicable to the weaned pig. The invention also discloses the application of the growth promoting agent for the weaned pig to feeds for the weaned pig. The feed additive can be used for relieving weaning stress of the pig, increasing the average daily gain of the weaned pig by more than 230%, reducing the feed to gain ratio by more than 40%, reducing the incidence of diarrhea by more than 54% and remarkably improving the growth performance of the pig, causes no resistance to drugs, can be used for effectively replacing an antibiotic growth promoting agent and satisfies a healthy breeding development direction.

The present disclosure relates to a composition for treating anxiety disorder, particularly to a pharmaceutical composition comprising N-acetyl-L-cysteine or its derivatives for treating anxiety disorder such as post traumatic stress disorder or phobia. In accordance with the present disclosure, N-acetyl-L-cysteine can block the renewal of fear memories for a sustained period of time when it was administered during or after an exposure therapy to treat post traumatic stress disorder or phobia.

The invention belongs to the technical field of assisted reproduction, in particular to a one-step embryo culture medium. A composition for preparing the one-step embryo culture medium is prepared from(+ / -)-alphal-lipoic acid, N-acetyl-L-cysteine according to a molar ratio of (0.055-0.098) : (0.702 to 0.858). The one-step embryo culture medium prepared by using the composition keeps effective components secreted into the culture medium by an embryo, plays a role in protecting cells and restraining, decomposing and utilizing harmful products, and the problem of low blastocyst forming rate caused by mechanical injury in the embryo transplanting process and stress reaction due to different culture environments is solved.