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Visual ratio fluorescent system for detecting cyanide ion, and preparation method and application of system

A technology of ratio fluorescence and cyanide ion, which is applied in the field of fluorescence chemical detection, can solve the problem of low sensitivity and achieve the effect of simple and cheap preparation

Active Publication Date: 2019-02-15
ZHEJIANG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the past 10 years, there have been many reports on the use of copper ions to quench quantum dots, and then the addition of cyanide ions to restore the fluorescence to detect cyanide ions, but most of them are less sensitive than fluorescent dyes.

Method used

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  • Visual ratio fluorescent system for detecting cyanide ion, and preparation method and application of system
  • Visual ratio fluorescent system for detecting cyanide ion, and preparation method and application of system
  • Visual ratio fluorescent system for detecting cyanide ion, and preparation method and application of system

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] To prepare a visual ratiometric fluorescence system for detecting cyanide ions, the steps are as follows:

[0033] 1) Preparation of NAC-modified CdTe quantum dots

[0034] First, add 0.004 mol / L CdCl to 100mL 2 Nitrogen was passed through the aqueous solution for 10 minutes, then 0.1371g NAC (Chinese name: N-acetyl-L-cysteine) was added to it, the pH of the mixed solution was adjusted to 11.0 with 1 mol / L NaOH solution, and then 0.2152 g trisodium citrate dihydrate, 0.0177 g Na 2 TeO 3 , 0.08 g NaBH 4 . Reflux reaction at 100°C for 11 h to obtain a red fluorescent NAC-modified CdTe quantum dot solution. Take 1mL of the prepared NAC-modified CdTe quantum dot solution and mix it with 2mL of ethanol, centrifuge and precipitate, take out the precipitate and disperse it in 1mL phosphate buffer (pH value 8), make a CdTe quantum dot solution and put it in the refrigerator Store at 4°C until use.

[0035] 2) Preparation of blue fluorescent carbon dots

[0036]Add 25 mL...

Embodiment 2

[0043] Investigate the effect of the pH of the phosphate buffer solution:

[0044] Prepare the phosphate buffer solution with pH 6.0, 6.4, 6.8, 7.0, 7.4, 7.6, 8.0, 8.4 and 8.8 in advance;

[0045] Take 14.1 μL of the CdTe quantum dot solution prepared in step 1) of Example 1, put it into 2.926 mL of the phosphate buffer with different pHs prepared above and shake well, perform fluorescence emission spectrum detection, and test the CdTe quantum dot solution without adding cyanide ions Fluorescence intensity versus pH change curve; then add 30 μL of 1 mmol / L cyanide ion aqueous solution, shake well and wait for 15 minutes to perform fluorescence emission spectrum detection, test the fluorescence intensity versus pH change curve after adding 10 μmol / L cyanide ion to the CdTe quantum dot solution. Thus, the ratio of the fluorescence peak intensity after adding 10 μmol / L cyanide ions to the CdTe quantum dot solution and the fluorescence peak intensity when no cyanide ions are added...

Embodiment 3

[0049] Examine the effect of quenching time:

[0050] Take 14.1 μL of the CdTe quantum dot solution obtained in step 1) of Example 1 and put it into 2.926 mL of phosphate buffer (pH value is 8), perform fluorescence emission spectrum detection, and test the fluorescence of the CdTe quantum dot solution without adding copper ions Peak intensity; then add 28.2μL of 0.1mol / L copper ion aqueous solution and shake well, and wait for 2, 4, 6, 8, 10, 20, 40, 60 minutes to measure the fluorescence emission spectrum (waiting time after adding copper ions The time is the quenching time), and the curve of the fluorescence peak intensity after adding copper ions to the CdTe quantum dot solution with the quenching time was tested. Take the quenching time as the abscissa, and the ratio of the fluorescence peak intensity of the corresponding CdTe quantum dot solution after adding copper ions to the fluorescence peak intensity when no copper ions are added is plotted on the ordinate, and the ...

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Abstract

The invention discloses a visual ratio fluorescent system for detecting a cyanide ion, and a preparation method and an application of the system. The visual ratio fluorescent system comprises an N-acetyl-L-cysteine modified CdTe quantum dot, a carbon dot, a copper ion and a phosphate buffer solution. In the visual ratio fluorescent system, the concentration of the copper ion is 0.7-1.2micromoles / L; the concentration of the N-acetyl-L-cysteine modified CdTe quantum dot is 20nmol / L; in a testing system, a ratio of fluorescent intensity of the carbon dot to fluorescent peak intensity of the N-acetyl-L-cysteine modified CdTe quantum dot quenched by the copper ion is (25-35):1; and a pH (potential of hydrogen) value of the visual ratio fluorescent system is 7-8.8. The visual ratio fluorescent system is simple and convenient to prepare, and cheap, and has good cyanide ion selectivity and sensitivity, a wide linear range and a very low limit of detection.

Description

technical field [0001] The invention relates to the field of fluorescence chemical detection, in particular to a visual ratio fluorescence system for detecting cyanide ions and its preparation method and application. Background technique [0002] Cyanide is a highly toxic substance. In daily life, cassava, nuts, cigarettes and other foods or daily necessities contain more cyanide. In industrial production, cyanide is widely used in chemical industry, metallurgy, pharmaceutical and other fields; Over the years, due to the illegal discharge of waste, waste gas, and waste liquid, cyanide ions may exist in river water, lake water, and tap water, which seriously endanger human health. [0003] The toxicity of cyanide comes from its high affinity with iron in cytochrome oxidase, so that the cells in the human body cannot get oxygen in time, resulting in headaches, coma, and even death. In addition, in addition to direct entry of cyanide into the human body, contact with the skin ...

Claims

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Application Information

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IPC IPC(8): G01N21/64C09K11/88C09K11/06C09K11/02
CPCC09K11/025C09K11/06C09K11/883G01N21/64G01N21/6428G01N21/643G01N2021/6421G01N2021/6432G01N2021/6439
Inventor 汪晶李大权黄亮胡军
Owner ZHEJIANG UNIV OF TECH
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