A Fluorescent Analysis Method Using Dual Emission Ratio Fluorescent Quantum Dot Probes for Detecting Dopamine

A ratiometric fluorescent probe and ratiometric fluorescence technology, applied in the field of preparation of environmental functional materials, can solve problems such as complex preparation, long time consumption, and harmfulness to organisms

Active Publication Date: 2021-01-05
JILIN NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, many inorganic nanoparticles cannot be degraded and are harmful to organisms. Therefore, in terms of inorganic fluorescent nanoparticles, how to overcome this difficulty has become a hot spot for many researchers.
Organic fluorescent materials are considered to have good prospects in terms of biodegradability, biocompatibility and modifiability, but there are still many problems, such as complex preparation, time-consuming and certain toxicity of raw materials

Method used

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  • A Fluorescent Analysis Method Using Dual Emission Ratio Fluorescent Quantum Dot Probes for Detecting Dopamine
  • A Fluorescent Analysis Method Using Dual Emission Ratio Fluorescent Quantum Dot Probes for Detecting Dopamine
  • A Fluorescent Analysis Method Using Dual Emission Ratio Fluorescent Quantum Dot Probes for Detecting Dopamine

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] (1) Add 60.6mg of sodium borohydride (NaBH4) and 51.04mg of tellurium powder into the centrifuge tube, use a needle to pierce a small hole on the cover of the centrifuge tube to discharge excess hydrogen from the reaction, and then add 1.5mL of double distilled water The solid was completely dissolved; the centrifuge tube was placed in an ultrasonic machine for ultrasonic reaction for 2 hours, and the final white transparent liquid was the required precursor NaHTe solution.

[0031](2) Under the condition of passing nitrogen and removing oxygen, inject the precursor NaHTe solution obtained in step (1) into cadmium chloride hydrate (CdCl2 2.5 H2O) aqueous solution, wherein 28.34 mg of CdCl2·2.5H2O2 was added, and the mixed solution was refluxed at 120° C. for 85 h under nitrogen protection conditions to obtain the required red fluorescent quantum dots.

[0032] (3) Add 800 μL of red fluorescent quantum dots obtained in step (2) to a mixed solution of 3.2 mL of n-hexanol,...

Embodiment 2

[0040] (1) Add 75.7mg of sodium borohydride (NaBH4) and 51.04mg of tellurium powder into the centrifuge tube, use a needle to pierce a small hole on the cover of the centrifuge tube to discharge excess hydrogen from the reaction, and then add 1.5mL of double distilled water The solid was completely dissolved; the centrifuge tube was placed in an ultrasonic machine for ultrasonic reaction for 2 hours, and the final white transparent liquid was the required precursor NaHTe solution.

[0041] (2) Under the condition of passing nitrogen and removing oxygen, the precursor NaHTe solution obtained in step (1) is injected into cadmium chloride hydrate (CdCl2 2.5 H2O) aqueous solution, wherein 152.23 mg of CdCl2·2.5H2O was added, and the mixed solution was refluxed at 120° C. for 85 h under nitrogen protection to obtain the required red fluorescent quantum dots.

[0042] (3) Add 800 μL of red fluorescent quantum dots obtained in step (2) to a mixed solution of 4.0 mL of n-hexanol, 15.2...

Embodiment 3

[0050] (1) Add 90.9mg of sodium borohydride (NaBH4) and 51.04mg of tellurium powder into the centrifuge tube, use a needle to pierce a small hole on the cover of the centrifuge tube to discharge excess hydrogen from the reaction, and then add 1.5mL of double distilled water The solid was completely dissolved; the centrifuge tube was placed in an ultrasonic machine for ultrasonic reaction for 2 hours, and the final white transparent liquid was the required precursor NaHTe solution.

[0051] (2) Under the condition of nitrogen deoxygenation, the precursor NaHTe solution obtained in step (1) is injected into cadmium chloride hydrate (CdCl2 2.5H2O) in the presence of 133 μL thioglycolic acid (TGA) for deoxygenation through nitrogen. ) aqueous solution, wherein 182.67 mg of CdCl2·2.5H2O was added, and the mixed solution was refluxed at 130° C. for 72 hours under the protection of nitrogen to obtain the required red fluorescent quantum dots.

[0052] (3) Add 800 μL of red fluorescen...

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Abstract

The invention relates to a fluorescence analysis method for detecting dopamine by virtue of dual-emission rate fluorescent quantum dot probe. According to the fluorescence analysis method, PDA with green fluorescence is prepared through reaction of a polyethyleneimine solution with a certain concentration and a dopamine solution with a certain concentration and is taken as a response signal of a rate fluorescence probe, r-QDs@SiO2 is taken as a stable interior label of the fluorescence probe, then PDA and r-QDs@SiO2 are assembled to obtain a dual-emission rate fluorescent sensor, and the concentration of dopamine is detected by virtue of the dual-emission rate fluorescence analysis method.

Description

technical field [0001] The invention relates to a fluorescence analysis method using double-emission ratio fluorescent quantum dot probes for detecting dopamine, which belongs to the technical field of environmental functional material preparation. Background technique [0002] Dopamine (DA) is a catecholamine neurotransmitter that often appears in the central nervous system, hormones and cardiovascular system, and is secreted by the human central nervous system. It is involved in human behavioral responses and brain functions, such as sensory and neural information transmission. play an important role. Abnormal concentrations of dopamine in biological systems can cause a series of neurological diseases and cause great harm to human health. Commonly, excessive secretion of dopamine can lead to energy metabolism failure and sudden neurological death; too little dopamine secretion can lead to loss of muscle control, inability to concentrate and even Parkinson's syndrome. The...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64
CPCG01N21/6428
Inventor 于淼谭振江逯洋于晓鹏
Owner JILIN NORMAL UNIV
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