Method for detecting lipase activity

A technology of lipase and activity, which is applied in the field of analytical chemistry, can solve the problems of complex preparation of solutions, dependence, and inconspicuous color changes, and achieve the effects of large-scale use, reduced experimental errors, and good water solubility of substrates
CN103063597AActive Publication Date: 2013-04-24NANJING UNIV OF TECH
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
NANJING UNIV OF TECH
Publication Date
2013-04-24

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Abstract

The invention provides a method for detecting lipase activity. The method comprises the following steps: mercaptoacetic acid methyl ester is utilized for modifying nanogold; in the presence of lipase, mercaptoacetic acid is generated by hydrolysis, and thus carboxylate radicals are exposed; intense hydrogen-bond interaction exists among carboxylate radicals, and thus the distance between nanogold is shortened to gather and blue the nanogold. On one hand, the strength of the lipase activity can be directly identified by naked eyes from the color and on the other hand, the lipase activity can be sensitively detected by an ultraviolet and visible spectrophotometer. The method can be used for detecting the lipase activity from the aspects of time, temperature, quantity and the like and the experimental phenomenon is obvious. The method has the advantages of being simple to operate without substrate emulsification, small in dosage, being beneficial to being obviously observed by naked eyes and low in cost. The method has not only good research value academically, but also wide application in production application.
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Description

technical field

[0001] The invention belongs to the field of analytical chemistry and relates to a method for detecting lipase activity. Background technique

[0002] Lipase is a large class of biocatalysts that can catalyze various chemical reactions such as hydrolysis, esterification, and transesterification. It has chemical, stereo, and site selectivity, high activity, few side reactions, and no cofactors. Widely used in many industrial fields such as food processing, biomedicine, biodiesel, fine chemicals, etc. Compared with ordinary chemical catalysts, it has the characteristics of high efficiency, stability, mildness and cleanliness, so it is a kind of "green" with great potential Therefore, it is very important to establish a convenient and fast method for detecting lipase activity.

[0003] At present, there are many methods for detecting lipase activity, such as physical and chemical methods (detecting the amount of reacted substrate and the amount of products prod...

Claims

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