A preparation method of ratiometric fluorescent probe for quantitative detection of ppi content in urine
A ratiometric fluorescent probe and fluorescent probe technology, applied in the field of detection of PPi in urine, can solve problems such as detection performance limitations
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0030] Example 1 The synthesis of blue fluorescent B-CDs, the steps are as follows:
[0031] Weigh 300mg of sodium citrate and 30mg of glutathione, add 10ml of water, ultrasonicate to fully dissolve it, transfer the solution to a 30ml reactor for sealing, and react at 200°C under high temperature and high pressure for 4h, the obtained After cooling, the solution was dialyzed with a 500Da dialysis bag for 12 h to obtain blue fluorescent B-CDs. Its transmission electron microscope image is as follows figure 1 In the middle A, the UV absorption and fluorescence emission diagrams are shown in figure 1 shown in B.
Embodiment 2
[0032] Embodiment 2 Synthesis of Y-CDs with yellow fluorescence, the steps are as follows:
[0033] Weigh 100 mg of 1,2,4-triaminobenzene, add 10 ml of formamide solution, stir to make 1,2,4-triaminobenzene evenly dispersed in the formamide solution, transfer the solution to a 30 ml reaction kettle, The reaction was carried out at 120 °C for 12 h, the solution obtained was centrifuged to remove unreacted substances, the supernatant was collected and purified by dialysis with a 500 Da dialysis bag for 12 h, and the obtained product was filtered with a 0.22 μm microporous membrane to obtain yellow fluorescent Y- CDs solution. Its transmission electron microscope image is as follows figure 2 In the middle A, the UV absorption and fluorescence emission diagrams are shown in figure 2 shown in B.
Embodiment 3
[0034] Example 3 Investigating the effect of B-CDs on Fe 3+ To respond, the steps are as follows:
[0035] Disperse 10 μL of B-CDs solution in 180 μL of pH=4 Tris-HCl buffer, and then add 10 μL of Fe with different concentrations 3+ The final concentration of B-CDs was 50 μM, 100 μM and 200 μM. After incubation at room temperature for 10 min, the fluorescence intensity of the solution was detected to investigate the effect of B-CDs on Fe 3+ response, the experimental results are as image 3 shown.
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com