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A dual-emission ratiometric quantum dot fluorescent probe and its preparation method and application

A fluorescent probe and quantum dot technology, applied in the field of dual emission ratio quantum dot fluorescent probes and their preparation, can solve the problems of cumbersome sample pretreatment, time-consuming, poor selectivity and the like

Inactive Publication Date: 2017-06-27
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The object of the present invention is to provide a kind of double-emission ratiometric quantum dot fluorescent probe and its preparation method for visual detection of aspirin, to overcome the large amount of solvent consumption in the detection of aspirin process in the prior art, time-consuming, loaded down with trivial details sample pretreatment and Disadvantages such as poor selectivity

Method used

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  • A dual-emission ratiometric quantum dot fluorescent probe and its preparation method and application
  • A dual-emission ratiometric quantum dot fluorescent probe and its preparation method and application
  • A dual-emission ratiometric quantum dot fluorescent probe and its preparation method and application

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Experimental program
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Effect test

Embodiment 1

[0030] (1) 30.3 mg sodium borohydride (NaBH 4 ) and 51.04 mg of tellurium powder were added to the centrifuge tube, and then 3.0 mL of double distilled water was added to dissolve the solid completely; the centrifuge tube was placed in an ultrasonic machine for ultrasonic reaction, and the tube mouth was kept to vent gas, and the final white liquid was the desired The precursor NaHTe solution.

[0031] (2) Inject the newly obtained precursor NaHTe into the CdCl with thioglycolic acid (TGA) at a pH of 10.5 for nitrogen and deoxygenation 2 In aqueous solution, to which 228.34 mg of CdCl was added 2 2.5H 2 O and 138.5 μL of TGA. Mixed solution under nitrogen protection 100 oUnder the condition of C, the reflux reaction was performed for 9 hours and 72 hours to obtain the desired green fluorescent quantum dots and red fluorescent quantum dots.

[0032] (3) Mix 40 mL of the red fluorescent quantum dot stock solution obtained in step (2) and 80 mL of ethanol into the flask, sti...

Embodiment 2

[0039] (1) 60.6 mg sodium borohydride (NaBH 4 ) and 51.04 mg of tellurium powder were added to the centrifuge tube, and then 3.0 mL of double distilled water was added to dissolve the solid completely; the centrifuge tube was placed in an ultrasonic machine for ultrasonic reaction, and the tube mouth was kept to vent gas, and the final white liquid was the desired The precursor NaHTe solution.

[0040] (2) Inject the newly obtained precursor NaHTe into the CdCl with thioglycolic acid (TGA) at a pH of 11.5 for nitrogen and deoxygenation 2 in aqueous solution to which 152.23 mg of CdCl was added 2 2.5H 2 O and 115.44 μL of TGA. The mixed solution was protected under nitrogen at 110 o Under the condition of C, the reflux reaction was performed for 9 hours and 72 hours to obtain the desired green fluorescent quantum dots and red fluorescent quantum dots.

[0041] (3) Mix 40 mL of the red fluorescent quantum dot stock solution obtained in step (2) and 100 mL of ethanol into th...

Embodiment 3

[0048] (1) Add 45.4 mg sodium borohydride (NaBH 4 ) and 51.04 mg of tellurium powder were added to the centrifuge tube, and then 3.0 mL of double distilled water was added to dissolve the solid completely; the centrifuge tube was placed in an ultrasonic machine for ultrasonic reaction, and the tube mouth was kept to vent gas, and the final white liquid was the desired The precursor NaHTe solution.

[0049] (2) Inject the newly obtained precursor NaHTe into CdCl with thioglycolic acid (TGA) at a pH of 11.2 for nitrogen and deoxygenation 2 in aqueous solution to which 182.672 mg of CdCl was added 2 2.5H 2 O and 133 μL of TGA. Mixed solution under nitrogen protection 105 o Under the condition of C, the reflux reaction was performed for 9 hours and 72 hours to obtain the desired green fluorescent quantum dots and red fluorescent quantum dots.

[0050] (3) Mix 40 mL of the red fluorescent quantum dot stock solution obtained in step (2) and 96 mL of ethanol into the flask, stir...

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Abstract

The invention relates to a dual-emission ratiometric quantum dot fluorescent probe for visually detecting aspirin and its preparation method and application, belonging to the technical field of material preparation and drug content detection; the preparation method of the probe is to firstly mix sodium borohydride, tellurium powder and water in an ultrasonic environment to generate a precursor NaHTe solution, and then inject the precursor into CdCl in the presence of thioglycolic acid 2 2.5H 2 O aqueous solution, reflux reaction under nitrogen protection conditions, to obtain the required green fluorescent quantum dots and red fluorescent quantum dots; red fluorescent quantum dots are coated with silicon spheres by sol-gel method, and connected with amino groups; green fluorescent quantum dots The solution and silicon-coated red fluorescent quantum dots are dispersed in MES buffer solution, added with EDC / NHS solution, and reacted in the dark at room temperature to obtain a double emission ratio quantum dot fluorescent probe, which is used for fluorescence quantitative and visual analysis to determine the content of aspirin; The quantum dot fluorescent probe prepared by the method has good optical properties and stability, and has the ability to visually detect aspirin.

Description

technical field [0001] The invention relates to a double-emission ratio quantum dot fluorescent probe and its preparation method and application, belonging to the technical field of material preparation and drug content detection. Background technique [0002] Aspirin (aspirin), also known as acetylsalicylic acid (acetylsalicylic acid), is the earliest, most widely used and most common antipyretic and analgesic antirheumatic drugs. It has various pharmacological effects such as antipyretic, analgesic, anti-inflammatory, anti-rheumatic and anti-platelet aggregation, etc. Arthritis and toothache etc. It has the characteristics of rapid drug effect, stable drug effect, and easy diagnosis and treatment of overdose. However, with the widespread use of aspirin, its adverse reactions are gradually increasing. The more common symptoms are nausea, vomiting, upper abdominal discomfort or pain, etc., so it is very important to monitor the amount of aspirin. At present, chromatograph...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64
Inventor 卫潇戴江栋李洪吉周志平闫永胜
Owner JIANGSU UNIV
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