Sputum specimen homogenization method and preparation of reagent for medical detection of sputum specimen

Abstract

The invention provides a sputum specimen homogenization method and preparation of a reagent for medical detection of a sputum specimen. The invention further provides a novel sputum digestion solution conforming to clinical detection, and the novel sputum digestion solution is prepared from 0.2% (w / v) of N-acetyl-L-cysteine, 0.1% (w / v) of trypsin, 0.4% (w / v) of triethanolamine, 0.2% (w / v) of potassium chloride, 1.5% (w / v) of potassium dihydrogen phosphate, 0.2% (w / v) of dipotassium hydrogen phosphate and a sterile water solvent.

Description

technical field [0001] The invention provides a new type of sputum digestive liquid that meets clinical testing, which can effectively homogenize sputum, protect bacteria in the sputum from damage, and facilitate further analysis of sputum samples. Background technique [0002] Clinically, natural expectoration and suction methods are generally used to collect sputum samples. The sputum samples obtained in this way have obvious heterogeneity, and the distribution of bacteria and other components is very uneven. With the development of medical testing and the improvement of laboratory quality control requirements, more and more attention has been paid to the pretreatment of specimens. According to daily work observations, the positive rate of sputum specimen culture is generally high, but the clinical coincidence rate is not ideal, and the actual pathogenic bacteria in the sputum often cannot be detected, which misleads the clinical diagnosis and treatment. [0003] At prese...

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Problems solved by technology

[0004] Although the above-mentioned sputum digestive juices and digestion methods have been used for the detection of sputum samples, the results of clinical practice show that the homogenization performance of these digestive juices and the ability to protect the bacteria in the samples are still lacking

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