Barley stripe disease pathogenic gene pgsln and application thereof

A disease-causing gene and disease-causing technology, applied in the fields of application, genetic engineering, plant gene improvement, etc., can solve the problems such as the report of pgsln gene and function of barley stripe disease, and achieve the effect of efficient breeding

Active Publication Date: 2021-01-29
GANSU AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Problems existing in the prior art: there is no report on the pgsln gene and function of barley stripe disease (Sclerotinia tritici) in the prior art

Method used

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  • Barley stripe disease pathogenic gene pgsln and application thereof
  • Barley stripe disease pathogenic gene pgsln and application thereof
  • Barley stripe disease pathogenic gene pgsln and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] The present embodiment provides a barley stripe disease pathogenic gene pgsln, the gene full-length sequence such as the sequence table SEQID NO: 1 and figure 1 with Figure 19 shown.

Embodiment 2

[0054] This embodiment provides a method for cloning the pgsln gene, the causative gene of barley streak disease, and its gene function verification method, comprising the following steps:

[0055] 1. Cloning and sequence analysis of pgsln gene

[0056] Carry out single spore isolation to barley streak WT bacterial strain, mycelia plate culture is in PDA medium, culture temperature 23-25 ​​℃, mycelia liquid culture is in PD medium, 23-25 ​​℃, 180rpm. Mycelia were cultured for DNA extraction, RNA extraction and protoplast preparation. The sequence of Saccharomyces cerevisiae sln (GenBank ID: NP EDV09625) was homologously compared with the results of barley streak WT genome sequencing (to be published), and the homologous sequence of sln was found, which was named pgsln, and the pgsln gene sequence was homologous Source clones, PCR amplification using cDNA and DNA as templates, and primer pair pgsln-F1 / pgsln-R1 (see Table 1) as primers to amplify the target fragment, the produc...

Embodiment 3

[0084] The present embodiment provides the application of a kind of barley stripe disease pathogenic gene pgsln gene in regulating mycelial growth rate, and the verification of this application comprises the following steps:

[0085] 1. Cloning and sequence analysis of pgsln gene

[0086] Single spores were isolated from the WT strain of barley stripe disease, and the mycelia were cultured on PDA medium at a culture temperature of 23°C, and the mycelium liquid was cultured on PD medium at 23°C and 180rpm. The mycelia were cultured for DNA extraction, RNA extraction and protoplast preparation. The sequence of Saccharomyces cerevisiae sln (GenBank ID: NP EDV09625) was homologously compared with the sequencing results of barley stripe disease WT genome, and the homologous sequence of sln was found, which was named pgsln, and the pgsln gene sequence was homologously cloned by PCR The amplification uses cDNA and DNA as templates, and the primer pair pgsln-F1 / pgsln-R1 (see Table 1)...

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Abstract

The invention provides a barley stripe disease pathogenic gene pgsln and an application thereof, belongs to the field of biotechnology engineering, and particularly provides a new gene pgsln derived from barley stripe disease sclerotinia sclerotiorum. The new gene pgsln can regulate and control the growth and development of barley streak pathogen filaments, mutants are knocked out through the pgsln gene, a large batch of mutant plants capable of resisting barley streak pathogens are cultivated, invasion of barley streak diseases can be efficiently solved, and the barley streak disease-resistant plants are simply, easily and efficiently cultivated. The new gene pgsln is related to osmotic stress and heavy metal stress regulation and control, and can improve the tolerance of barley stripe disease pathogenic bacteria plants to iprodione, carbendazim and fludioxonil and participate in the integrity of cell walls, i.e., the gene can regulate and control the coping capacity of the plants toadversity stress, and the pgsln transgenic crops are cultivated, so that the environment can be protected; and sustainable development of agriculture is facilitated.

Description

technical field [0001] The invention belongs to the field of biotechnology engineering, in particular to pgsln, a causative gene for barley stripe disease and its regulation of mycelial growth rate, participating in the regulation of osmotic pressure stress, and improving the tolerance of barley stripe disease bacteria to iprodione, carbendazim and fludioxonil properties, regulation of mycelial cell wall integrity, and applications related to pathogenicity of barley stripe disease. Background technique [0002] Barley leaf stripe (Barley leaf stripe) is one of the main diseases of barley, which occurs widely in barley growing areas. In recent years, due to changes in farming systems and years of continuous cropping, the disease has become more and more serious. It is particularly important to study the pathogenic mechanism of the pathogen. It is now known that the disease is a systemic invasive fungal disease caused by seed-carrying bacteria, and the asexual stage of its pa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/31C12N15/82A01H5/00A01H6/46
CPCC07K14/37C12N15/8282Y02A50/30
Inventor 姚立蓉王化俊梁倩倩汪军成孟亚雄李葆春马小乐司二静杨轲边秀秀马增科
Owner GANSU AGRI UNIV
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