Regulatory gene PdeMIXTA02 for initial development of floc of Poplus deltoides and application of the regulatory gene PdeMIXTA02

A technology for regulating genes and black poplar, applied in the fields of application, genetic engineering, plant genetic improvement, etc., can solve problems that have not been well understood

Active Publication Date: 2020-05-19
NANJING FORESTRY UNIV
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  • Abstract
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AI Technical Summary

Problems solved by technology

However, the molecular mechanism of poplar catkin production has not been well understood

Method used

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  • Regulatory gene PdeMIXTA02 for initial development of floc of Poplus deltoides and application of the regulatory gene PdeMIXTA02
  • Regulatory gene PdeMIXTA02 for initial development of floc of Poplus deltoides and application of the regulatory gene PdeMIXTA02
  • Regulatory gene PdeMIXTA02 for initial development of floc of Poplus deltoides and application of the regulatory gene PdeMIXTA02

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Embodiment 1

[0027] (1) Determination of the initial development site and time point of poplar catkins

[0028] On the campus of Nanjing Forestry University (32° north latitude, 118° west longitude), the female plant of Nanlin 895 fast-growing poplar (NL895), the main species of American black poplar, which has grown for more than 15 years, was selected.

[0029] In March 2018, the female inflorescence florets of NL895 poplar female flowers were collected 1 day after pollination (1DPA), 3 days after pollination (3DPA) and 5 days after pollination (5DPA), placed in FAA fixative solution, and vacuumized. The female florets preserved in the fixative were planed longitudinally, dehydrated with gradient alcohol concentrations, dried, and observed with a scanning electron microscope. The results showed that poplar catkins began to protrude from the epidermal cells of the placenta at the 1DPA time point, and poplar catkin cells elongated rapidly after the 3DPA time point ( figure 1 ). This phys...

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Abstract

The invention discloses a regulatory gene PdeMIXTA02 for initial development of floc of Poplus deltoides and application of the regulatory gene PdeMIXTA02, and belongs to the technical field of plantgenetic engineering. Through gene sequence evolution analysis and gene expression analysis at different time points before and after initial development of poplar floc and combination of verificationof the genetic transformation function of Arabidopsis thaliana, the gene PdeMIXTA02 for control on initial development of poplar floc is disclosed firstly. The gene belongs to a family of MIXTA transcription factors, it is shown through sequence evolution analysis that the gene and key genes GhMML3 and GhMML4 for regulating the initial development of cotton wadding are in the same branch of MIXTAsubclasses, and the gene only has specific expression within 5 days after pollination of female floc of Poplus deltoides, the amount of epidermal hair can be increased significantly through excessiveexpression in Arabidopsis thaliana, and bump differentiation of epidermal cells of Arabidopsis thaliana into epidermal hair is promoted; and it is confirmed through the verification that the gene PdeMIXTA02 for regulating the initial development of poplar floc can be applied to breeding of new poplar varieties without fly floc.

Description

technical field [0001] The invention belongs to the technical field of plant genetic engineering, and more specifically relates to a regulatory gene PdeMIXTA02 for the initial development of poplar catkins of American black poplar (Poplus deltoides) and an application thereof. Background technique [0002] Poplar (Populus spp.) is the most widely distributed and most adaptable tree species in the world. Poplar cultivation in China is mainly concentrated in North China, Central China, Northeast China and Northwest China. Poplar is not only an important fast-growing and high-yielding timber tree species, but also an important tree species for environmental afforestation; due to its extremely fast growth rate and good cell wall chemical properties, it is also a suitable raw material for the production of second-generation biofuels; its habitat adaptability is strong , and can also be widely used in ecological shelterbelts, three-north shelterbelts, and agricultural and forestr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/29C07K14/415C12N15/82A01H5/00A01H6/00
CPCC07K14/415C12N15/8213C12N15/8218C12N15/8261
Inventor 吴怀通周芳伟尹佟明
Owner NANJING FORESTRY UNIV
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