A chemoenzymatic synthesis method of a blood group trisaccharide

Through chemical enzymatic synthesis method, the reaction of enzyme α1,2-FucT and glycosyltransferase was adopted, combined with the enzyme removal solvent treatment and purification steps, and the blood type trisaccharide A and B were successfully prepared, solving the scarcity and expensive problems of blood type trisaccharide synthesis in the prior art, and providing key raw materials to support multi-field applications.

CN119876304BActive Publication Date: 2025-07-25WUHAN TANGZHI PHARM CO LTD
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Patent Information

Application Number
CN202510369392.7
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2025-03-27
Publication Date
2025-07-25
Estimated Expiration
2045-03-27

AI Technical Summary

Technical Problem

The chemical enzymatic synthesis method of blood type trisaccharide A and B in the prior art has not been reported, resulting in scarce market supply and expensive, which cannot meet research and application needs.

Method used

The chemical enzyme synthesis method is adopted, including steps S1 to S6, and blood type trisaccharides A and B are prepared by the reaction of enzyme α1,2-FucT and glycosyltransferase, combined with the enzyme removal solvent treatment and purification steps.

Benefits of technology

The efficient and direct preparation of blood type trisaccharides A and B is achieved, solving the problem of low yield and expensive prices, and providing key raw materials for sugar-protein/antibody research, coupling drug development, in vitro diagnosis and medical device consumables.

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Abstract

The present invention provides a chemoenzymatic synthesis method for blood group trisaccharide, which includes: dissolving a substrate, GDP-Fuc, and magnesium chloride in water, adjusting the pH of the solution, adding the enzyme α1,2-FucT, and stirring for reaction to obtain a first product reaction solution; adding a de-enzyme solvent, standing, centrifuging and filtering, taking the supernatant, and evaporating to remove the de-enzyme solvent to obtain an aqueous solution of the first product; adding a glycosylated product, adjusting the pH after dissolution, and adding a glycosyltransferase to obtain a second product reaction solution; using a de-enzyme solvent to remove the glycosyltransferase, standing, centrifuging and filtering, taking the supernatant, and evaporating to remove the de-enzyme solvent to obtain an aqueous solution of the second product; separating and purifying the aqueous solution of the second product, and freeze-drying to obtain a solid product; dissolving the solid product, adding Pd / C, introducing hydrogen gas, stirring and filtering to remove Pd / C, concentrating and freeze-drying to obtain the blood group trisaccharide. This method provides a new technical idea for the chemoenzymatic synthesis of blood group trisaccharide, which is conducive to popularization and application and solves the problems of low yield and high price.
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