Method for detecting activity of spermidine/spermine N1-acetyltransferase

Abstract

The invention, relating to the technical field of medicine, relates to a method for detecting the activity of spermidine/spermine N1-acetyltransferase, that is, a quantitative technique of analyzing SSAT activity through detecting acetylated metabolites of the SSAT substrate. According to the invention, the N-acetylation is regulated mainly through or only through SSAT in SSAT pathway and the specific substrate in the N-acetylation regulation is L-dopa, partial metabolism of the substrate can generate an acetylated metabolite N-acetyl-L-dopa thought the introduction of SSAT, the SSAT upregulation is positively correlated with cancer, accordingly, L-dopa can be used as a cancer mark regulated by the SSAT upregulation, and a novel detection method is provided for early detection, early diagnosis, early treatment and the like of tumors. The method has the advantages of safely, efficiency, strong practicality, convenient and fast operating method, simple usage, high positive incidence, and remarkable effect, and can be used as an auxiliary means for preventing, diagnosing, detecting, protecting, treating and studying various tumors.

Description

technical field [0001] The present invention relates to the field of medical technology, in particular to a medical detection technology, more specifically to a spermidine / spermine N 1 -A method for acetyltransferase (abbreviation: SSAT) activity, and more specifically relates to a method for analyzing SSAT activity by detecting acetylated metabolites of SSAT substrates, wherein: the SSAT substrate is levodopa, levodopa Dopa is partially metabolized by induction of SSAT to produce the acetylated metabolite N-acetyl-L-dopa. Background technique [0002] (1) Research overview of levodopa [0003] 1 Overview [0004] 3-Hydroxy-L-tyrosine / L-β-(3,4-dihydroxyphenyl)alanine / L-3-(3,4-dihydroxyphenyl)alanine / L-(3,4 -Dihydroxyphenyl)alanine (Levodopa), also known as L-dopa (abbreviation: L-Dopa, L-DOPA), is the hydroxylation of tyrosine, which is the intermediate product of L-tyrosine synthesis of catecholamines in the body . As a drug, the common name is levodopa, also known as ...

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Problems solved by technology

[0045] 4. Pharmacology and Toxicology

[0049] ①The curative effect is better for mild and younger patients, but poor for severe and elderly patients;

[0050] ② It has better curative effect on muscle stiffness and dyskinesia, but poor curative effect on muscle tremor symptoms, such as long-term medication and larger doses can still be effective on the latter;

[0051] ③The effect is slow, and it usually takes 2 to 3 weeks to improve the objective signs, and the maximum curative effect is obtained after 1 to 6 months, but the effect is long-lasting and increases with the prolongation of the medication time

[0084] (3) Adverse reactions

[0096] ③ Monoamine oxidase inhibitors can prevent the degradation of dopamine in the body and enhance its effect, but they can lead to rapid heart rate and hypertensive crisis, so they should not be used together with this product

[0097] ④ It should not be used together with reserpine and adrenomimetic drugs

[0142] In conclusion, the analysis of existing technical data such as literature search shows that so far, no reports have been found on SSAT activity, enzyme kinetics, and acetylation assay methods of SSAT spermidine and spermine substrates.

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