Liquid injection and suction method for cell slide-making dyeing machine and device thereof

Abstract

The invention discloses a liquid injection and suction method for a cell slide-making dyeing machine. The liquid injection and suction method comprises the following steps of 1) injecting a proper amount of a body fluid sample, which is required for detection, in a sampling bottle onto a glass slide through a liquid injection pipe arranged on the cell slide-making dyeing machine in a manner of approaching one side of a round pipe wall, and tilting the glass slide towards one side of the round pipe wall approaching the liquid injection pipe during liquid injection; 2) sucking away redundant waste liquid through a liquid suction pipe arranged on the cell slide-making dyeing machine in a manner of approaching the bottom of one side of the round pipe wall, and tilting the glass slide towards one side of the round pipe wall approaching the liquid suction pipe; 3) injecting dyeing liquid required for dyeing through the liquid injection pipe arranged on the cell slide-making dyeing machine in a manner of approaching one side of the round pipe wall, stewing the dyeing liquid, and titling the glass slide towards one side of the round pipe wall approaching the liquid injection pipe; 4) sucking away the redundant waste liquid through the liquid suction pipe arranged on the cell slide-making dyeing machine in a manner of approaching the bottom of one side of the round pipe wall after cells are colored, tilting the glass slide towards one side of the round pipe wall approaching the liquid suction pipe when the redundant waste liquid is sucked away, and repeating the step 3) and the step 4) for 2-8 times until the dyeing operation is finished.

Description

technical field [0001] The invention relates to the field of staining of cell slices, in particular to a method for injecting and absorbing liquid for a cell slice staining machine and a device thereof. Background technique [0002] The staining process of cell slices is divided into two steps: slice preparation and staining. Preparation is to extract the amount required for detection from the body fluid specimens placed in the sampling bottle, such as cervical exfoliated cells, sputum, urine, pleural ascites, etc., through the specimen transfer tube on the cell preparation and staining machine, and inject it into the slide in the air ; A round tube with a diameter smaller than the width of the slide is tightly fixed on the slide, and the two form a temporary container to prevent the loss of the liquid injected on the slide; after standing for a period of time, the cells under the action of gravity A thin layer of cells is formed on the glass slide, and then the remaining w...

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Problems solved by technology

[0003] The above process is completed by the cell staining machine, and the defects are: 1. Since the dye solution is injected in the air, the dye solution is directly hit on the cell layer of the slide at the bottom of the round tube, so it is possible to adhere to the slide. The cells break up, destroying the uniformity of the cell layer

The remaining waste liquid accounts for 20-40% of the dyeing solution, which leads to changes in the composition of the dyeing solution added next time, and the ideal staining of the cells cannot be performed. After the staining is completed, the background is dirty and disordered , the cell staining is not clear, which affects the pathologist's diagnosis

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