Fungus detection fluorescent dyeing liquid and use thereof

A fluorescent dyeing and fungal technology, applied in the field of medical testing, can solve the problems of inconspicuous contrast between tissue background and fungal coloration, low positive detection rate, etc., and achieve the effect of easy to find and identify, strong versatility, and excellent dyeing effect.

Inactive Publication Date: 2016-12-07
JIANGSU LIFETIME BIOLOGICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Xu Hong and Gu Julin (Medical Fungi Examination. Chinese Journal of Mycology, 2006, 1(2): 111-116) used direct microscopic examination for fungal examination, and the formula of compound potassium hydroxide solution used included potassium hydroxide, dimethyl sulfoxide (DMSO), glycerin, adding DMSO to the formula to promote cutin dissolution, glycerin smears are not easy to dry, and it is not easy to make potassium hydroxide crystals, but using this formula to make products, there are still tissue background and fungal color contrast are not obvious, positive detection low rate issues

Method used

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  • Fungus detection fluorescent dyeing liquid and use thereof
  • Fungus detection fluorescent dyeing liquid and use thereof
  • Fungus detection fluorescent dyeing liquid and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] 1. The formula composition is as shown in Table 2.

[0045] Table 2

[0046]

[0047] 2. The preparation method is as follows:

[0048] Dyeing liquid A preparation:

[0049] (1) Solution A: Use a precision analytical balance to weigh the amount of fluorescent whitening agent 28, add deionized water to dissolve.

[0050] (2) Liquid B: Weigh the formula amount of potassium hydroxide and add deionized water to dissolve it. Slowly add glycerin and dimethyl sulfoxide in proportion to the weight of the formula while stirring at 20°C until completely dissolved. Allow the liquid to cool below 40°C.

[0051] (3) While liquid B is constantly stirring (80-100 rpm), add liquid A dropwise. After the addition is complete, continue stirring for 5 minutes. Weigh and add volatile water to the formula amount. Seal container mouth tightly.

[0052] Stand at room temperature in the dark for 24 hours, then pack.

[0053] Dyeing solution B preparation:

[0054] (1) Weigh the formu...

Embodiment 2600

[0064] Example 2 600 cases of clinical specimen detection positive rate comparison

[0065] The specimens of 600 routine patients are respectively adopted the formula described in the embodiment of the present invention 1 and the background technology and Gram staining to carry out fungal detection, and the results are shown in the following table:

[0066] table 3

[0067]

[0068]

[0069] Using this product and traditional Gram staining method to stain fungal infection samples, the staining results of this product ( figure 2 ) The background color difference is large, and the distinction is obvious. The combination of fungi and fluorescein completely emits fluorescence, which is very easy to distinguish. Traditional Gram stain ( image 3 ) background is not easy to distinguish, and the fungal morphology cannot be distinguished, so it is easy to be ignored. Thereby verifying the superiority and effectiveness of this product. In addition, compared with the KOH wet ...

Embodiment 3

[0070] Example 3 Stability test of fungal fluorescent staining solution

[0071] The stability test was carried out on the fluorescent staining solution in the following concentration range.

[0072] Table 4

[0073]

[0074] 1. Thermal stability test:

[0075] Put the sample of the fluorescent staining solution to be tested in a water bath at 80°C for one week, take it out, and observe with the naked eye that there is no stratification of the liquid; drop a drop on a glass slide, cover it with a cover glass, and observe under a microscope, no crystals are precipitated; use an inoculation loop to take a little red Put the trichophyton on a glass slide, drop a drop of solution A, and after 1 minute, put the first drop of solution B, cover with a cover glass, absorb the excess liquid with absorbent paper after light pressure, observe under a fluorescent microscope, the hyphae turn blue Fluorescence, the internal structure is clear, the background is free of impurities, and ...

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Abstract

The invention discloses a fungus detection fluorescent dyeing liquid and a use thereof. The fungus detection fluorescent dyeing liquid comprises an independent dyeing liquid A and an independent re-dyeing liquid B. The dyeing liquid A comprises, by weight, 0.02-0.043% of a fluorescent brightener, 1-10% of potassium hydroxide, 10-20% of dimethyl sulfoxide, 0-5% of glycerin and 65-87% of water. The re-dyeing liquid B comprises, by weight, 0.1-0.5% of Evans blue and 99.50-99.90% of water. The invention discloses a use of the fungus detection fluorescent dyeing liquid. The invention provides a novel fast fungus infection detection product. The product can be used for detecting various fungi which may exist in a fresh or frozen clinical sample and paraffin and ethylene glycol methacrylate-coated tissue.

Description

technical field [0001] The invention belongs to the field of medical inspection, and in particular relates to a fluorescent staining solution for fungal detection and its application. Background technique [0002] Fungi are a type of eukaryote. The most common fungi are various types of mushrooms, but fungi also include molds and yeasts. More than 70,000 species of fungi have now been discovered, estimated to be only a small half of all that exist. Most fungi were originally classified as animals or plants. [0003] At present, fungi have become independent kingdoms in taxonomy, paralleling the kingdoms of animals, plants, prokaryotes and protists. Fungi have solid cell walls and true nuclei, do not contain chlorophyll, are heterotrophic, live in a parasitic or saprophytic manner, and typically reproduce both sexually and asexually, producing spores of various forms. According to the growth characteristics and morphological differences, fungi can be simply divided into y...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64G01N1/30
CPCG01N21/6486G01N1/30G01N2001/302
Inventor 王学锋潘为民董春丽陈晔
Owner JIANGSU LIFETIME BIOLOGICAL TECH
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