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Method and kit for detecting cervical carcinoma by using PAX1 and CDH1 gene methylation

A kit and methylation technology, applied in the medical field, can solve the problems of high missed detection rate, unclear relationship between cervical cancer occurrence and difficult popularization

Inactive Publication Date: 2010-09-15
上海市第八人民医院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0013] Although many tumor suppressor genes or proto-oncogenes are known, it is unclear which genes are closely related to cervical cancer
If 10 or more genes need to be tested at the same time to give a test result, it may be difficult to popularize the test due to the high cost
On the other hand, if the few genes identified cannot cover the vast majority of patients, then the missed detection rate may be too high

Method used

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  • Method and kit for detecting cervical carcinoma by using PAX1 and CDH1 gene methylation
  • Method and kit for detecting cervical carcinoma by using PAX1 and CDH1 gene methylation
  • Method and kit for detecting cervical carcinoma by using PAX1 and CDH1 gene methylation

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Embodiment 2

[0125] Kit preparation

[0126] Prepare a cervical cancer detection kit, which contains methylation-specific restriction endonucleases Hpa II, BstuI and HhaI each 200 units, and contains each 100 microliters of specific primers, each of which is listed in Table 1. Each primer SEQ ID NO: 1-8. The kit also contains instructions for use.

[0127] All documents mentioned in this application are incorporated by reference in this application as if each were individually incorporated by reference. In addition, it should be understood that after reading the above teaching content of the present invention, those skilled in the art can make various changes or modifications to the present invention, and these equivalent forms also fall within the scope defined by the appended claims of the present application.

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Abstract

The invention relates to a method and a kit for detecting cervical carcinoma by using PAX1 and CDH1 gene methylation. Particularly, the invention provides a kit, which contains (a) a primer pair aiming at methylation specificities and non-methylation specificities of a POX1 gene and a CDH1 gene, or contains (b) a methylation sensitivity restriction endonuclease, and a primer pair aiming at the specificities of the POX1 gene and the CDH1 gene. The kit can effectively separate the cervical carcinoma from cervicitis and HPV infection.

Description

technical field [0001] The invention relates to the field of medicine, and more specifically relates to a method and a kit for detecting cervical cancer or cervical cancer susceptibility by using the methylation status of genes PAX1 and CDH1 related to cervical carcinogenesis. Background technique [0002] For a long time, people's views on the inheritance of living organisms have been described in the language of DNA (that is, with 4 base nucleotides, adenosine (G), cytosine (C) and thymine (T) as symbols). of. Both the maintenance and divergence of phenotypes in living organisms are due to the faithful or mutated intergenerational transmission of carrier DNA (in some cases, in the form of RNA) carrying the genes determining these phenotypes. The phenotypic diversity of diploid organisms can also be greatly enhanced by the combination of paternal and maternal genomes. However, accumulating evidence indicates that epigenetic mechanisms, not involving structural changes at ...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
Inventor 徐军王红琳
Owner 上海市第八人民医院
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