The invention belongs to the technical field of optical
microscopic imaging, and particularly relates to a single objective lens light sheet
microscopic imaging system, which comprises an exciting
light modulation light path, a
fluorescence detection light path and a
microscopic imaging system, and is characterized in that the exciting
light modulation light path is used for generating an illumination light sheet to excite
fluorescence, and specifically comprises a
laser, a beam expanding collimating lens group, a modulator, a scanning
system and the like. The microscopic imaging system is used for transmitting an illumination light sheet and collecting light emitted by a sample, and comprises a dichroscope, a
galvanometer module, an image rotating system, a tubular lens, an objective lens and the like. And the
fluorescence detection light path comprises a
relay lens group, a modulation element, a band-pass
optical filter, a camera and the like. According to the invention, tasks of light sheet illumination and fluorescence collection in a traditional double-objective light sheet
microscope are completed through a single objective, a remote focusing system or a micro-
mirror reflection structure commonly used in an existing single-objective light sheet
microscope does not need to be used, and a light sheet orthogonal to a focal plane is adopted for illumination. Compared with the existing light sheet
microscope technology, the method has higher
usability and flexibility, allows free switching of objective lenses under the condition of not changing other light paths, is compatible with any objective lens and various samples, does not need any
software processing including deskewing in the principle of an obtained image, can directly display and use an original image, and is high in practicability. The system has higher resolution and is close to ideal isotropic imaging when various objective lenses are used for imaging.