21 results about "Mapk signaling pathway" patented technology

This invention discloses a coumarin compound and its uses, belonging to the field of chemical and pharmaceutical technology. This invention provides coumarin compounds represented by Formula I, particularly some specific coumarin compounds. These compounds and their compositions can inhibit the MEK or Ras-MAPK signaling pathway, providing a new approach for treating diseases related to the MEK or Ras-MAPK signaling pathway.

The present invention relates to the technical field of chemicals and pharmaceuticals. Disclosed are a coumarin compound and a use thereof. The present invention provides a coumarin compound represented by formula I, and some specific coumarin compounds. The compound and composition can effectively inhibit MEK or Ras-MAPK signaling pathways, can be used for preparing MEK or Ras-MAPK signaling pathway inhibitors, and provide a new way for treating MEK or Ras-MAPK signaling pathway-related diseases.

This invention relates to the field of plant disease biological control technology, and particularly to a Fusarium oxysporum cell wall extract, its preparation method, and its application. The extract is derived from the polysaccharide fraction of the cell wall of Fusarium oxysporum spores or hyphae, and contains at least one pathogen-associated molecular pattern (PAMP) component. This component is a non-protein, thermally stable, species-nonspecific elicitor capable of forming an aqueous dispersion system. The extract of this invention can be recognized by plant PRRs and activate PTI immunity. Rehmannia glutinosa tubers treated with the extract showed a significantly increased germination rate after inoculation with Fusarium oxysporum. It also induces superoxide anion production, upregulates the activities of superoxide dismutase, peroxidase, and catalase, and enriches differentially expressed genes in the MAPK signaling pathway, jasmonic acid metabolism pathway, and phenylpropane biosynthesis pathway. Furthermore, the extract can directly inhibit Fusarium oxysporum colony expansion, conidia production, and mycelial growth. Extracts derived from spores or hyphae show no significant difference in inducing disease resistance, and the raw materials are flexible and easy to industrialize. This extract is derived from the cell wall of the pathogen itself, making it environmentally friendly and free from the risk of chemical pesticide residues. It is suitable for the green control of root rot disease in Rehmannia glutinosa.

The application discloses a caviar polypeptide composition with enhanced immune function, a preparation method, application and preparation, belongs to the technical field of bioactive polypeptides, and comprises at least one polypeptide with an amino acid sequence of RWFPGKPLFWRA, FWKPLRGALFPW or KWRFPGALPWFA and a molecular weight of 1400-1550 Da. The preparation method comprises caviar protein extraction, complex enzymolysis, ultrafiltration separation, chromatography purification and solid-phase synthesis. Animal experiments prove that the polypeptide can significantly enhance cell immunity, humoral immunity, monocyte-macrophage function and NK cell activity, the mechanism is related to TLR2 / TLR4 receptor activation and NF-κB / MAPK signal pathways, the safety is good, and the polypeptide is suitable for development into an immune-enhancing health food or medicine.

This invention belongs to the field of active ingredient extraction and purification technology, specifically a neutral polysaccharide ORP-0 from *Agaricus bisporus* and its applications. The preparation method of this invention includes the following steps: (1) pretreatment; (2) polysaccharide extraction; (3) impurity removal; and (4) separation and purification. This invention obtains a single neutral polysaccharide ORP-0 through defatting pretreatment, ultrasonic-assisted extraction, multiple impurity removal processes, and separation and purification using a DEAE-52 cellulose chromatography column. The method of this invention is simple to operate, has mild reaction conditions, and a complete impurity removal process. The obtained neutral polysaccharide has high purity, with a total sugar content of 92.09%. Furthermore, the obtained neutral polysaccharide ORP-0 enhances the activity of RAW 264.7 macrophages, increases the release of immune factors, enhances the expression of phosphorylated proteins in the MAPK signaling pathway, and activates the body's immune response.

The application discloses a benzofuran derivative, which has a structure shown in formula (I), and discloses a pharmaceutical composition and application in preparation of anti-inflammatory drugs. The application can effectively inhibit LPS-induced inflammation in vivo and in vitro, and can play an anti-inflammatory role by inhibiting the release of inflammatory factors TNF-alpha and IL-6 through inhibition of NF-kappa B and MAPK signal pathways.

The invention belongs to the technical field of biological medicine and pharmacology, and particularly relates to EGCG-5-HT binary oxidation self-agglutination nanoparticles as well as a preparation method and application of the EGCG-5-HT binary oxidation self-agglutination nanoparticles. The EGCG (epigallocatechin gallate)-5-HT binary oxidation auto-agglutination nanoparticles disclosed by the invention are obtained by carrying out oxidation auto-agglutination on EGCG and 5-HT in an alkaline environment. The total concentration of the EGCG and the 5-HT in the reaction liquid is 1.0 to 3.0 mg / mL. The nanoparticles provided by the invention have an anti-oxidation effect, and can inhibit pyroptosis and regulate and control a cell cycle; an NF-kappa B signal channel is inhibited, and the anti-inflammatory effect is achieved; an MAPK signal channel is activated, and cell apoptosis is inhibited; a PI3K-Akt signal channel is activated, and cell proliferation and mucous membrane repair are promoted. When the compound is applied to preparation of IBD medicines, multi-mechanism synergistic treatment of anti-inflammatory, anti-oxidation and mucous membrane repair of IBD is realized; the invention also provides a preparation method of the nanoparticle, and the preparation method is mild in condition and simple.

The invention belongs to the technical field of medicines, and particularly relates to application of traditional Chinese medicine active ingredients in antianaphylaxis, in particular to application of rare ginsenoside Rh1 in preparation of medicines for preventing or treating anaphylaxis. According to the invention, the action mechanism of Rh1 in resisting anaphylaxis is systematically clarified by integrating network pharmacology, molecular docking, molecular dynamics simulation and transcriptomics technologies for the first time. The Rh1 acts on four key host targets, namely ALB, CASP3, NFKB1 and STAT3 at the same time, regulates and controls an MAPK signal channel, a Th17cell diffusion channel and a PI3K-Akt signal channel, and synergistically exerts the dual effects of inhibiting allergens from invading host cells and relieving cytokine storm. The Rh1 and the four key targets all have high affinity binding. The invention provides a brand new theoretical basis and a clear mechanism support for developing Rh1-based broad-spectrum, high-efficiency and low-toxicity anti-anaphylaxis drugs.

This invention discloses a sustained-release membrane for postoperative treatment of diabetic peripheral neuropathy and its preparation method. The sustained-release membrane comprises, from the outside in, a PLGA nanofiber outer layer containing tacrolimus and magnesium hydroxide nanoparticles, a glucose-responsive hydrogel middle layer containing insulin and glucose oxidase / catalase, and a PCL-oriented nanofiber inner layer containing methylcobalamin. The layers are connected by a polydopamine interface layer, and the outermost surface of the outer layer is coated with a hyaluronic acid anti-adhesion coating. Insulin, as a local neurotrophic factor, directly activates the PI3K / Akt / mTOR and ERK / MAPK signaling pathways, promoting axonal regeneration and myelin repair. The sustained-release membrane integrates three major functions: immunomodulation / anti-inflammation, glucose-responsive insulin neurotrophic delivery, and sustained nerve regeneration promotion. It prevents postoperative nerve re-adhesion through a spatiotemporal sequential drug release strategy. All components are biodegradable and do not require secondary surgery for removal.

The present application relates to a kind of drug based on PARM1 regulation treatment calcified aortic valve disease and its application.The present application first finds that PARM1 is significantly increased in the aortic valve of calcified aortic valve disease (CAVD) patient, and plays a key role in the osteogenic differentiation process of valve interstitial cells (VICs), and the expression level of PARM1 is knocked down can inhibit the osteogenic differentiation of VICs and extracellular calcium salt deposition, reduce aortic valve calcification, and its mechanism of action involves MAPK signal pathway.The present application provides a new target and treatment idea for the treatment of CAVD, and can be applied to related drug research and development and treatment method development.

The present invention provides a use of a ugonin compound for the treatment of joint-related diseases by enhancing chondrogenesis. More specifically, the chondrogenesis is mediated by the ugonin compound’s ability to promote the increase of aggrecan and type II collagen, which is achieved through the inhibition of miR-3074-5p and the activation of the MAPK signaling pathway.

The invention discloses a low-salt dry-cured mackerel polypeptide as well as a preparation method and application thereof in anti-inflammation, and belongs to the technical field of fermented food. According to the method, lactobacillus plantarum is inoculated, the addition amount of table salt in the pickling process is reduced, the low-salt dry-pickled mackerel is prepared, and 10 gamma-glutamic acid containing peptides are extracted through separation and purification. Experimental results show that the 10 gamma-glutamic acid containing peptides all have different degrees of anti-inflammatory potentials. Wherein the gamma-glutamic acid-containing peptide with the amino acid sequence shown as SEQ ID NO.2 can significantly down-regulate the phosphorylation level of NF-kB, JNK, P38MAPK and ERK in RAW264.7 mouse inflammation model cells, so that an MAPK signal channel is inhibited, massive secretion of an inflammatory mediator NO and pro-inflammatory cytokines IL-6 and TNF-alpha is inhibited, and the gamma-glutamic acid-containing peptide has outstanding anti-inflammatory activity and can be developed into anti-inflammatory drugs.

This invention discloses an acidic polysaccharide from Gynostemma pentaphyllum, AGPA12, its purification and extraction method, and its application in the preparation of anti-pancreatic cancer drugs. AGPA12 is mainly composed of mannose, glucuronic acid, rhamnose, glucose, galactose, and fucose, with a molar ratio of 1.05:1.74:6.29:1.40:69.48:19.03. The polysaccharide was extracted using an α-amylase-assisted water extraction and alcohol precipitation method, and purified by DEAE-Sepharose FF ion exchange chromatography and Sepharose CL6B gel chromatography. In vitro experiments showed that AGPA12 had significant inhibitory effects on the proliferation and migration of human pancreatic cancer AsPC1 cells, and its mechanism was related to the regulation of the MAPK signaling pathway and the expression of apoptosis-related proteins. This invention provides a new approach for the development of Gynostemma pentaphyllum polysaccharides and their application in anti-pancreatic cancer drugs.

The application of TAB2 protein in rabies treatment target spot belongs to the field of biological medicine technology. In order to study the influence of host protein on RABV replication, further study the pathogenic molecular mechanism of RABV, and find new rabies treatment target and drug, the application uses fluorescence quantification, Western Blotting and other methods to study the influence of host protein TAB2 on RABV replication, and finds that TAB2 gene transient knockdown or stable knockdown can inhibit the replication of rabies virus, and further finds that TAB2 protein can be used as a new target for rabies treatment. In addition, the application also finds the structure domain of TAB2 and RABV M interaction and the TAB2 protein promotes RABV replication by activating TAK1-p38 / MAPK signal pathway, and the p38 inhibitor Gossypetin and the TAK1 inhibitor Takinib can be used as potential drugs for treating RABV infection.

The application discloses application of a large-mouth black bass stomach polypeptide in preparation of an antioxidant and / or immunoregulation product, relates to the field of bioactive peptides, and is characterized in that the large-mouth black bass stomach polypeptide is a polypeptide product obtained by alkaline protease enzymolysis of large-mouth black bass stomach, and components with molecular weights less than 3000 Da account for more than 98% of the total peptide amount. The polypeptide has significant DPPH free radical, hydroxyl free radical and ABTS cationic free radical scavenging capacity and reducing capacity, can promote macrophage proliferation, enhance phagocytosis, promote nitric oxide secretion, up-regulate immune-related cytokine expression, and play an immunoregulation role through activation of P38 and P44 MAPK signal pathways. The application realizes high-value utilization of large-mouth black bass processing by-products, has high product safety, and has a wide application prospect.

The invention discloses application of a D-dopa pigment isomerase inhibitor in preparation of a medicine for treating rheumatoid arthritis, and belongs to the technical field of biological medicine. The invention defines that D-DT is an independent and key driving factor for rheumatoid arthritis, especially for the pathological behavior of fibroblast-like synovial cells. The specific molecular mechanism that D-DT plays a role in FLS through an MAPK signal path-COX-2 / PGE2 signal axis is systematically clarified for the first time, and a complete'target spot-pathway-function 'chain is formed. In-vitro and in-vivo experiments prove that selective inhibition of D-DT can effectively relieve disease symptoms and pathological progression, and solid principle verification is provided for development of a new therapy.

The invention belongs to the technical field of traditional Chinese medicine extraction, and particularly relates to a furocoumarin compound as well as a preparation method and application thereof. The furocoumarin compound provided by the invention has a structure as shown in a formula I. The furocoumarin compound provided by the invention can inhibit the release of inflammatory factors, has an anti-oxidation effect, and also can inhibit NF-kappa B and MAPK signal pathways, so that the furocoumarin compound plays an anti-inflammatory role and is used for preparing anti-inflammatory drugs. Moreover, the furocoumarin compound is extracted from radix angelicae, the source is natural, and the preparation method is simple and efficient. Formula I

The invention discloses genes ZjAGL11 and ZjAGL15 for regulating and controlling development of jujube embryos and application thereof, and relates to the technical field of plant genetic engineering, and the technical key points are as follows: the ZjAGL11 and the ZjAGL15 play a regulating and controlling role in plant development and participate in plant hormone signal transduction, energy metabolism and MAPK signal pathway biological processes, so that gene expression related to embryonic development is regulated, and the development of the jujube embryos is regulated. The reproductive development of plants is influenced; the gene provides a potential gene regulation strategy for improving seed development.

The invention relates to an application of a milk-derived peptide KEMPFPK in the aspects of immune balance regulation and inflammation relief. The peptide can effectively inhibit the activation of downstream NF-kB and MAPK signal channels by targeting a TLR2 / 4 receptor on the surface of a macrophage, significantly relieve immune organ structure damage caused by cyclophosphamide, systematically down-regulate the levels of IL-6, IL-1beta, TNF-alpha, IgA and IgM in serum, and increase IL-10 at the same time, so that the multi-level anti-inflammatory and immunoregulation effects are achieved. The peptide can be used for preparing anti-inflammatory drugs, immunomodulatory functional foods and nutritional supplements, and has good safety and application prospects.

This invention discloses the application of PACAP peptides as plant immunomodulators, belonging to the field of plant immunology technology. This invention is the first to apply PACAP to a plant system, discovering that it can act as a novel plant immunomodulator to regulate plant immune responses, thereby enhancing plant resistance to pathogenic microorganisms. Experimental results show that PACAP can rapidly induce intracellular calcium ion influx in Arabidopsis thaliana cells, activate the MAPK signaling pathway, and upregulate the expression of early immune marker genes such as FRK1. Furthermore, PACAP treatment significantly improves plant resistance to pathogens such as *Pseudomonas syringae* and *Ralstonia solanacearum*. This invention also confirms that not only does the full-length PACAP (1-38) possess plant immune-promoting activity, but its N-terminal truncated peptide (1-27) and receptor antagonist fragment (6-38) also have plant immune-inducing functions. This invention provides a new technical solution for the development of plant immune inducers.

The application belongs to the technical field of traditional Chinese medicine extraction, and particularly relates to a furanocoumarin compound, a preparation method and application thereof. The furanocoumarin compound provided by the application has a structure shown in formula I. The furanocoumarin compound provided by the application can inhibit the release of inflammatory factors, has an antioxidation effect, can inhibit the NF-kappa B and MAPK signal pathways, thereby playing an anti-inflammatory role, and is used for preparing anti-inflammatory drugs. Moreover, the furanocoumarin compound is extracted from radix angelicae dahuricae, is natural in origin, and is simple and efficient in the preparation method. Formula I.