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Application of Lefty protein in restraining fibrosis lesion of organ

A technology for liver fibrosis and kidney fibrosis, applied in the fields of molecular biology and cell signal transduction, can solve problems such as reducing collagen matrix production and increasing collagen matrix degradation

Inactive Publication Date: 2008-04-02
WUHAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Generally speaking, these treatment methods (plans) have their own shortcomings, either only reduce the production of collagen matrix, or only increase the degradation of collagen matrix, and there is almost no "two-pronged" treatment from these two aspects at the same time as a whole means

Method used

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  • Application of Lefty protein in restraining fibrosis lesion of organ
  • Application of Lefty protein in restraining fibrosis lesion of organ

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Experimental program
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Embodiment Construction

[0058] 1. Preparation method of Lefty protein

[0059] A.transfection of leftyA gene: select GP+E86 cells (purchased from American Type Culture Collection, ATCC) with DMEM medium containing 10% fetal bovine serum and 100U / ml penicillin, 100 μg / ml streptomycin, as Contains 5% CO 2 The cells were cultured in a 37°C incubator, and the cells were passaged by trypsin (Invitrogen, Inc.) digestion. Cells were transfected using Lipofectamin 2000 (Invitrogen, Inc.) transfection reagent. Cells were subcultured 24 hours before transfection and seeded in 35 mm diameter Petri dishes. Transfection can be carried out when the cells cover 30%-60% of the plate. Firstly, take 4 μg lefty-A / pcDNA1 plasmid and dilute it with 150ul DMEM medium without serum and double antibody, mix well, and place at room temperature. Take 6 μl of Lipofectamin 2000 liposomes, dilute with 150 μl of serum-free and double-antibody-free DMEM medium, mix well, and incubate at room temperature for 5 minutes. Add the...

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Abstract

The invention discloses the application of a Lefty protein in prohibiting fibering pathologic change of organs, in basic research on preparing the medicine for antagonizing TGF-beta, Nodal, BMP, p38MAPK signal pathways, and in preparing the medicine for curing or preventing fibering pathologic change of organs and tissues of liver, kidney, lung, cardiac muscle, skin, etc. The invention has the functions of prohibiting signal pathways (such as TGF-beta / Smad, Nodal signal pathways) related to fibering diseases and activating protein hydrolytic ferment (such as MMP, gelatingase, etc) capable of prohibiting fibering, is a biological preparation with great potential clinical application value, and can help to further probe into the occurring mechanism of fibering diseases.

Description

technical field [0001] The invention belongs to the fields of molecular biology and cell signal transduction. It involves the use of Lefty protein to inhibit fibrotic lesions in various organs and tissues. On the one hand, the protein down-regulates tissue collagen by inhibiting fibrosis-related signal transduction pathways (transforming growth factor [TGF]-β, Nodal, etc.). On the other hand, it promotes the decomposition of fibrotic tissue by up-regulating the activity of various proteolytic enzymes. At the same time, it relates to the preparation method of the protein, and the application of the protein in mechanism research and clinical medicine for the treatment or prevention of liver, kidney, lung, heart muscle, skin and other organs and tissue fibrosis. Background technique [0002] Chronic fibrosis of important organs induced by various reasons is often accompanied by progressive decline in organ function, eventually leading to organ failure and even patient death. C...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/17A61P1/16A61P9/00A61P11/00A61P13/12A61P17/00C12N15/63C12P21/02
Inventor 张杰姚颐
Owner WUHAN UNIV
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