Application of Chinese soft-shelled turtle cathelicidin-ps-cath4 peptide in the preparation of anti-inflammatory drugs

A ps-cath4, Chinese soft-shelled turtle technology, applied in the field of biomedicine, can solve the problems of no reports, and achieve the effects of simple structure, low cytotoxicity and hemolytic activity, and good stability
CN104725499BActive Publication Date: 2017-12-26DALIAN UNIV OF TECH
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
DALIAN UNIV OF TECH
Publication Date
2017-12-26

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Abstract

The invention discloses application of Chinese softshell turtle Cathelicidin-Ps-CATH4 peptide in preparation of an anti-inflammatory drug, and belongs to the technical field of biomedicine. Ps-CATH4 is linear peptide, and contains 32 amino acid residues; RT-PCR displays that the peptide has an inhibition effect on expression of critical inflammatory factors induced by lipopolysaccharide and an inducible NO synthase gene; and on the protein level, the peptide has a negative regulation effect on LPS-induced inflammatory factors and NO release, has an ascending effect on expression of inflammatory factors, and is mainly used for inhibiting release of the inflammatory factors by inhibiting activation of a TLR4 acceptor and a downstream MAPK signal channel. The peptide is simple in structure, does not contain a disulfide bond or a cyclic structure; chemical synthesis and genetic engineering preparation are facilitated; the primary medicinal evaluation shows low cytotoxicity and hemolytic activity; and the peptide is good in stability on salt, blood serum, temperature and time, and is applied to preparation of the anti-inflammatory drug.
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Description

Technical field

[0001] The invention provides a Chinese soft-shelled turtle ( Pelodiscus sinensis ) Cathelicidin-Ps-CATH4 and its coding gene, amino acid sequence and application in the preparation of anti-inflammatory drugs belong to the field of biomedical technology. Background technique

[0002] Cathelicidin is a family of multifunctional immunomodulatory peptides composed of the N-terminal signal peptide region, the middle conserved cathelin domain and the C-terminal highly specific mature peptide region. It is found in almost all kinds of animals (mammals, birds, Amphibians and fish) have been found. Like defensins, Cathelicidin is a host defense peptide unique to most vertebrates, including humans, and constitutes a key component of vertebrate natural immune response. The bridge between natural immunity and specific immunity plays an extremely important role in the innate immune system of animals. In addition to its broad-spectrum antibacterial activity, Cathelicidin also...

Claims

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