Direct venous re-transfusion immune cell cryopreservation medium and application thereof
A technology of immune cells and cryopreservation solution, applied in the field of biomedicine, which can solve the problems of cumbersome cryopreservation process and subsequent application procedures, unclear components of cryopreservation solution, and unsuitability for large-scale production, so as to facilitate clinical application and ensure safety Effectiveness and effectiveness, good effect of cryopreservation
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[0033] Example 1: Preparation and application of immune cell cryopreservation solution for direct intravenous infusion
[0034] 1. Raw materials and specifications
[0035] Hydroxyethyl starch and sodium chloride injection: 500ml: Hydroxyethyl starch (200 / 0.5) 30g
[0036] Dextran 40 glucose injection: 500ml: 30g dextran 40 and 25g glucose
[0037] Heparin Sodium Injection: 2ml: 12,500 units
[0038] Human albumin injection: 50ml: 10g total protein
[0039] 2. Preparation and application of immune cell cryopreservation solution for direct intravenous infusion
[0040] 1) Prepare liquid A and liquid B separately
[0041] A solution (100ml): 8.3ml dimethyl sulfoxide + 45.8ml hydroxyethyl starch sodium chloride injection + 45.8ml dextran 40 glucose injection + 0.1ml heparin sodium injection.
[0042] The content of each component in A solution is: dimethyl sulfoxide 8.3v / v%, hydroxyethyl starch 2.75w / v%, dextran 2.75w / v%, glucose 2.29w / v%, heparin sodium 625U / ml, The rest are medical injectio...
Example Embodiment
[0056] Example 2: Preparation and application of immune cell cryopreservation solution for direct intravenous infusion
[0057] 1. Raw materials and specifications
[0058] Hydroxyethyl starch and sodium chloride injection: 500ml: Hydroxyethyl starch (200 / 0.5) 30g
[0059] Dextran 40 glucose injection: 500ml: 30g dextran 40 and 25g glucose
[0060] Heparin Sodium Injection: 2ml: 12,500 units
[0061] Human albumin injection: 50ml: 5g total protein
[0062] 2. Preparation and application of immune cell cryopreservation solution for direct intravenous infusion
[0063] 1) Prepare liquid A and liquid B separately
[0064] A solution (100ml): 10ml dimethyl sulfoxide + 70ml hydroxyethyl starch sodium chloride injection + 19.9ml dextran 40 glucose injection + 0.1ml heparin sodium injection
[0065] The content of each component in A solution is: dimethyl sulfoxide 10v / v%, hydroxyethyl starch 4.2w / v%, dextran 1.19w / v%, glucose 1.0w / v%, heparin sodium 625U / ml, the rest For medical injection;
[006...
Example Embodiment
[0079] Example 3: Preparation and application of immune cell cryopreservation solution for direct intravenous infusion
[0080] 1. Raw materials and specifications
[0081] Hydroxyethyl starch and sodium chloride injection: 500ml: Hydroxyethyl starch (200 / 0.5) 30g
[0082] Dextran 40 glucose injection: 500ml: 30g dextran 40 and 25g glucose
[0083] Heparin Sodium Injection: 2ml: 12,500 units.
[0084] Human albumin injection: 50ml: 5g total protein
[0085] 2. Preparation and application of immune cell cryopreservation solution for direct intravenous infusion
[0086] 1) Prepare liquid A and liquid B separately
[0087] A solution (100ml): 9ml dimethyl sulfoxide + 33ml hydroxyethyl starch sodium chloride injection + 57.9ml dextran glucose injection + 0.1ml heparin sodium injection.
[0088] The content of each component in A solution is: dimethyl sulfoxide 9v / v%, hydroxyethyl starch 1.98w / v%, dextran 3.47w / v%, glucose 2.9w / v%, heparin sodium 625U / ml, the rest For medical injection;
[0089]...
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