Direct venous re-transfusion immune cell cryopreservation medium and application thereof
A technology of immune cells and cryopreservation solution, applied in the field of biomedicine, which can solve the problems of cumbersome cryopreservation process and subsequent application procedures, unclear components of cryopreservation solution, and unsuitability for large-scale production, so as to facilitate clinical application and ensure safety Effectiveness and effectiveness, good effect of cryopreservation
Active Publication Date: 2017-05-17
HARBIN MEDICAL UNIVERSITY
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The invention discloses a direct venous re-transfusion immune cell cryopreservation medium and application thereof. The direct venous re-transfusion immune cell cryopreservation medium is prepared from dimethyl sulfoxide, human serum albumin, dextran, hydroxyethyl starch, heparin sodium and other clinical medicinal level raw materials by optimizing the proportion of the dose of each raw material. In addition, the operation steps are simplified and the cryopreservation effect is improved by optimizing the cryopreservation method. On one hand, the potential risk of introducing an animal source antigenic substance in the cell treatment process is avoided, and on the other hand, the cryopreservation effect of immune cells is better than that of a conventional cryopreservation medium, thereby guaranteeing the safety and validity in clinical use. Two kinds of components are used for preservation, so that the cryopreservation medium can be preserved stably for a long time. The method disclosed by the invention is simple in operation, provides stronger operability for implementation of large-scale cryopreservation, and simultaneously provides a fundamental technical guarantee for implementation of immune cell treatment.
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