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Application of krait venom antibacterial peptide in preparation of medicine for resisting inflammatory factor

An antibacterial peptide and anti-inflammatory technology of golden ring snake, which can be applied in the field of biomedicine and can solve the problems of undiscovered public reports and the like

Inactive Publication Date: 2013-09-25
KUNMING INST OF ZOOLOGY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] Through literature search, do not find the public report identical with the present invention

Method used

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  • Application of krait venom antibacterial peptide in preparation of medicine for resisting inflammatory factor
  • Application of krait venom antibacterial peptide in preparation of medicine for resisting inflammatory factor
  • Application of krait venom antibacterial peptide in preparation of medicine for resisting inflammatory factor

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Embodiment Construction

[0031] The substantive content of the present invention is further described below with embodiment, but content of the present invention is not limited thereto.

[0032] 1. Antimicrobial peptides of ceratops inhibited the secretion of inflammatory factors induced by bacterial lipopolysaccharide (LPS) in vitro

[0033] Raw 264.7 cells were cultured in DMEM medium containing 10% fetal bovine serum to the logarithmic growth phase. When the state was good, they were digested with 0.25% trypsin, centrifuged at 900 rpm / min for 5 min, and then cultured with a medium containing 5% bovine serum albumin. Resuspend the cells in DMEM medium, adjust the number of cells so that the cell concentration reaches 1 × 10 6 / mL, after pipetting evenly, add to 96 cell culture well plate, 180 μl per well, at 37℃, 5% CO 2 Culture in the incubator for 3-4 h, add 2 μg / ml bacterial lipopolysaccharide and 20 μl of antimicrobial peptides of different concentrations after the cells adhere filter), conti...

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Abstract

The invention relates to an application of krait venom antibacterial peptide in the preparation of a medicine for resisting inflammatory factor and belongs to the field of biomedical technology. A lot of researches show that the krait antibacterial peptide has an obvious effect of inhibiting inflammatory factor secretion induced by lipopolysaccharide in vitro and inhibits inflammatory factor secretion through inhibiting MAPK signal channels; the krait venom antibacterial peptide has a remarkable scavenging activity of DPPH and 2,2-azine-bis(3-ethylbenzothiazole-6-sulfoacid)diammonium salt (ABTS+)radical in vitro and has an obvious effect of inhibiting mice inflammation induced by a complete Freund adjuvant in vivo; and the krait venom antibacterial peptide can be applied in the preparation of a medicine for resisting inflammatory factor.

Description

Technical field: [0001] The invention relates to the application of antibacterial peptides of crotonis venom in the preparation of anti-inflammatory factor medicines, and belongs to the technical field of biomedicine. Background technique: [0002] Antimicrobial peptides are found in almost all living things. Most antimicrobial peptides have broad-spectrum antibacterial activity, not only have very strong bactericidal activity against Gram-positive bacteria, Gram-negative bacteria, some fungi and viruses, but also have effects on many clinical drug-resistant bacteria. In addition, they also have many other biological activities, such as chemotaxis to various immune cells, induction of mast cell degranulation and histamine release, regulation of macrophage transcription, promotion of wound healing, induction of angiogenesis, induction of mutation Cell line apoptosis and lymphocyte activation, etc. Owing to having so many biological activities, antimicrobial peptides have al...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/17A61P29/00A61P39/06
Inventor 赖仭张治业李东升刘蕊
Owner KUNMING INST OF ZOOLOGY CHINESE ACAD OF SCI
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