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Application of naucleamide G in preparation of anti-inflammatory drug

An anti-inflammatory drug and inflammatory technology, applied in the field of medicine, can solve the problem of unclear active substances

Inactive Publication Date: 2017-01-25
JIANGSU PROVINCE INST OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The main process of danmu preparation is water decoction and alcohol precipitation, and the active substances in the preparation are not yet clear

Method used

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  • Application of naucleamide G in preparation of anti-inflammatory drug
  • Application of naucleamide G in preparation of anti-inflammatory drug
  • Application of naucleamide G in preparation of anti-inflammatory drug

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0019] Example: The compound of the present invention, Naucleamide G, has a good inhibitory effect on inflammation in LPS-stimulated RAW264.7 cells.

[0020] (1) Cell culture

[0021] Mouse RAW 264.7 macrophages were purchased from Shanghai Cell Bank, Chinese Academy of Sciences. at 37°C, 5% CO 2 Condition, cultured with DMEM incomplete medium containing 10% HyClone fetal bovine serum, 100 U / mL penicillin and 100 mg / mL streptomycin, digested with 0.5% trypsin for passage. All operations are aseptic.

[0022] (2) Cell Viability Experiment

[0023] RAW 264.7 cells were seeded in 96-well plates (4×10 6 1 / mL), six replicate wells in each group, and each well contained 200 μL of incomplete high-glucose DMEM medium. After the cells adhered, the cells were washed with PBS once, the control group was added with incomplete high-glucose medium, the induction group was added with incomplete high-glucose medium containing 1 μg / mL LPS, and the administration group was added with 25, 5...

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Abstract

The invention relates to the technical field of medicines, in particular to a novel compound which is separated from a medicinal fathead tree and is named as naucleamide G, and application of the novel compound in preparation of an anti-inflammatory drug. The naucleamide G can obviously inhibit generation of inflammatory factors IL-1beta and TNF-alpha in an LPS-stimulated cell RAW 264.7 and also inhibit expression of IL-1beta and TNF-alpha mRNA. The research on an anti-inflammatory mechanism shows that the naucleamide G can inhibit NF-kB and MAPK signal channels stimulated by LPS in the cell RAW 264.7.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to the application of a new alkaloid glycoside compound Naucleamide G isolated from the stem of the plant Galliflora in the preparation of anti-inflammatory drugs. Background technique [0002] Anti-inflammatory drugs generally exert their anti-inflammatory effects by regulating the levels of pro-inflammatory factors (TNF-α, IL-1β, IL-6, etc.) that play an important role in inflammation. Regarding the research on anti-inflammatory mechanism, the literature describes the role of many signaling pathways in inflammation (Guha M, Mackman N. LPS induction of gene expression in human monocytes. Cell Signal. 2001, 13: 85-94), among which, Nuclear factor-κB (NF-κB) and mitogen-activated protein kinase (MAPK) are two important pathways associated with inflammation. Studies have shown that NF-κB is mainly activated through the phosphorylation and degradation of IκB-α mediated by...

Claims

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Application Information

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IPC IPC(8): A61K31/706A61P29/00
CPCA61K31/706
Inventor 朱粉霞叶霁青陈家全翟小婷张振海
Owner JIANGSU PROVINCE INST OF TRADITIONAL CHINESE MEDICINE
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