Application of naucleamide G in preparation of anti-inflammatory drug

An anti-inflammatory drug and inflammatory technology, applied in the field of medicine, can solve the problem of unclear active substances

Inactive Publication Date: 2017-01-25
JIANGSU PROVINCE INST OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The main process of danmu preparation is water decoction and alcohol prec

Method used

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  • Application of naucleamide G in preparation of anti-inflammatory drug
  • Application of naucleamide G in preparation of anti-inflammatory drug
  • Application of naucleamide G in preparation of anti-inflammatory drug

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0019] Example: The compound of the present invention, Naucleamide G, has a good inhibitory effect on inflammation in LPS-stimulated RAW264.7 cells.

[0020] (1) Cell culture

[0021] Mouse RAW 264.7 macrophages were purchased from Shanghai Cell Bank, Chinese Academy of Sciences. at 37°C, 5% CO 2 Condition, cultured with DMEM incomplete medium containing 10% HyClone fetal bovine serum, 100 U / mL penicillin and 100 mg / mL streptomycin, digested with 0.5% trypsin for passage. All operations are aseptic.

[0022] (2) Cell Viability Experiment

[0023] RAW 264.7 cells were seeded in 96-well plates (4×10 6 1 / mL), six replicate wells in each group, and each well contained 200 μL of incomplete high-glucose DMEM medium. After the cells adhered, the cells were washed with PBS once, the control group was added with incomplete high-glucose medium, the induction group was added with incomplete high-glucose medium containing 1 μg / mL LPS, and the administration group was added with 25, 5...

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Abstract

The invention relates to the technical field of medicines, in particular to a novel compound which is separated from a medicinal fathead tree and is named as naucleamide G, and application of the novel compound in preparation of an anti-inflammatory drug. The naucleamide G can obviously inhibit generation of inflammatory factors IL-1beta and TNF-alpha in an LPS-stimulated cell RAW 264.7 and also inhibit expression of IL-1beta and TNF-alpha mRNA. The research on an anti-inflammatory mechanism shows that the naucleamide G can inhibit NF-kB and MAPK signal channels stimulated by LPS in the cell RAW 264.7.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to the application of a new alkaloid glycoside compound Naucleamide G isolated from the stem of the plant Galliflora in the preparation of anti-inflammatory drugs. Background technique [0002] Anti-inflammatory drugs generally exert their anti-inflammatory effects by regulating the levels of pro-inflammatory factors (TNF-α, IL-1β, IL-6, etc.) that play an important role in inflammation. Regarding the research on anti-inflammatory mechanism, the literature describes the role of many signaling pathways in inflammation (Guha M, Mackman N. LPS induction of gene expression in human monocytes. Cell Signal. 2001, 13: 85-94), among which, Nuclear factor-κB (NF-κB) and mitogen-activated protein kinase (MAPK) are two important pathways associated with inflammation. Studies have shown that NF-κB is mainly activated through the phosphorylation and degradation of IκB-α mediated by...

Claims

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Application Information

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IPC IPC(8): A61K31/706A61P29/00
CPCA61K31/706
Inventor 朱粉霞叶霁青陈家全翟小婷张振海
Owner JIANGSU PROVINCE INST OF TRADITIONAL CHINESE MEDICINE
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