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Separating and purifying method and application of codonopsis pilosula oligosaccharides

A technology of Codonopsis pilosula and oligosaccharides, applied in the field of separation and purification of Codonopsis oligosaccharides, which can solve the problems of little research on Codonopsis pilosula and no sweetness

Active Publication Date: 2019-07-19
LANZHOU UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Codonopsis polysaccharides, which have been studied more at present, have no sweetness; the main sweetness source of Codonopsis radix monosaccharides and oligosaccharides has little research

Method used

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  • Separating and purifying method and application of codonopsis pilosula oligosaccharides
  • Separating and purifying method and application of codonopsis pilosula oligosaccharides
  • Separating and purifying method and application of codonopsis pilosula oligosaccharides

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Experimental program
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Effect test

Embodiment 1

[0026] The inventor of the present application collected 40 batches of the three Codonopsis ginseng varieties stipulated in the Pharmacopoeia from the main producing areas of Codonopsis pilosula: Codonopsis pilosula (10 batches in the production area of ​​Gansu Province and 10 batches in Changzhi area of ​​Shanxi Province), Suhua Codonopsis (10 batches in the production area of ​​Wenxian County, Gansu Province) , Sichuan Codonopsis (10 batches in Tianning, Sichuan Province), by measuring the yield of polysaccharides and crude oligosaccharides of Codonopsis pilosula in different varieties and different production areas, it can be known that the content of crude oligosaccharides (RCPO) in Codonopsis pilosula in various places is 13.83-33.92g / 100g range, indicating that the oligosaccharide part is also an important component of Codonopsis pilosula. Comparing the in vitro antioxidant activities of different extracts of Codonopsis pilosula from the same origin ( image 3 ), in the ...

Embodiment 2

[0050] The extraction and separation method of Codonopsis oligosaccharides is:

[0051] Mix Codonopsis Codonopsis coarse powder and 50% ethanol, reflux extraction twice, each time for 0.5 hours, collect the filtrate and dregs respectively, concentrate the filtrate under reduced pressure to obtain Codonopsis pilosula alcohol extract (CPEE); add 12 Double the weight of water, decoct 3 times, 60 minutes each time, collect and combine the water extracts, and concentrate the water extracts to 1 / 2 volume under reduced pressure to obtain a concentrated solution; divide the concentrated solution into two parts, one part of the concentrated solution Concentrate under reduced pressure again to obtain Codonopsis pilosula water extract (CPWE); add ethanol to the other part of the concentrated solution until the final volume concentration is 80% for alcohol precipitation, and freeze-dry the precipitate to obtain Codonopsis pilosula polysaccharide (CPP); Concentrate the supernatant under red...

Embodiment 3

[0054] The extraction and separation method of Codonopsis oligosaccharides is:

[0055] Mix Codonopsis Codonopsis coarse powder and 95% ethanol, reflux extraction twice, each time for 1 hour, collect the filtrate and dregs respectively, concentrate the filtrate under reduced pressure to obtain Codonopsis pilosula alcohol extract (CPEE); add 8 Double the weight of water, decoct 3 times, 30 minutes each time, collect and combine the water extracts, and concentrate the water extracts to 1 / 4 volume under reduced pressure to obtain a concentrated solution; divide the concentrated solution into two parts, one part of the concentrated solution Concentrate under reduced pressure again to obtain Codonopsis pilosula water extract (CPWE); add ethanol to the other part of the concentrated solution until the final volume concentration is 80% for alcohol precipitation, and freeze-dry the precipitate to obtain Codonopsis pilosula polysaccharide (CPP); Concentrate the supernatant under reduced...

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Abstract

The present invention provides a separating and purifying method and an application of codonopsis pilosula oligosaccharides. Inventors find that the codonopsis pilosula oligosaccharides (CPO) can regulate immune activity through a MAPK signaling pathway in vivo and can also regulate macrophage immune activity through the MAPK signaling pathway in vitro.

Description

technical field [0001] The invention relates to a separation and purification method and application of Codonopsis oligosaccharides. Background technique [0002] As one of the most popular traditional Chinese medicines, Codonopsis pilosula has the functions of invigorating the middle and nourishing Qi, invigorating the spleen and lungs; carbohydrates are important components in Codonopsis pilosula, including polysaccharides, oligosaccharides and monosaccharides; The research on the structure and activity of Codonopsis mainly focuses on polysaccharides of Codonopsis pilosula; Codonopsis pilosula has been regarded as "good" since ancient times. It is recorded in "Baicao Mirror" that "Dangshen... is always better if it is clean, soft, strong, solid and sweet". "Yaolong Xiaopin" records that "Western produce is the best, the body is waxy and sweet, and the one with less residue is better." Codonopsis pilosula polysaccharides, which have been studied more at present, have no s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/702A61K31/7016A61K31/7004A61P37/02
CPCA61K31/702A61K31/7016A61K31/7004A61P37/02A61K36/344A61K2300/00
Inventor 胡芳弟白瑞斌苟于强王燕平马铭张静
Owner LANZHOU UNIVERSITY
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