Novel lactic acid bacterium lactobacillus fermentum isolated from adults in longevity village, helpful for defecation

A new technology of Lactobacillus fermentum, which is applied in the field of probiotics of new Lactobacillus fermentum strains and the above-mentioned strains, to achieve the effects of no antibiotic resistance transfer, improved defecation activity, acid resistance and bile resistance

Inactive Publication Date: 2016-07-13
PL BIO CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0010] On the other hand, in 2011, the Ministry of Food and Drug Safety analyzed the distribution of microorganisms in the intestines of residents living in rural health and longevity villages in South Korea and residents over the age of 40 in urban areas, and finally confirmed the following: Among the differences in the distribution of beneficial bacteria such as cocci between urban residents and those living in Changshou Village, the ratio of lactic acid bacteria to the whole intestinal bacteria was 0.56%: 1.355%, and the ratio of lactococcus to the whole intestinal bacteria was 0.02%: 0.1 %, which shows that compared with urban residents, the distribution of beneficial bacteria in Changshou Village residents is 3 to 5 times higher, but there are no lactic acid bacteria as follows: no possibility of transfer of antibiotic resistance, all meet the acid and acid resistance that probiotics should have Bile tolerance, ability to adhere to intestinal epithelium, inhibitory activity against pathogenic bacteria, antioxidant activity and immune enhancement effect, etc.

Method used

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  • Novel lactic acid bacterium lactobacillus fermentum isolated from adults in longevity village, helpful for defecation
  • Novel lactic acid bacterium lactobacillus fermentum isolated from adults in longevity village, helpful for defecation
  • Novel lactic acid bacterium lactobacillus fermentum isolated from adults in longevity village, helpful for defecation

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preparation example Construction

[0052] The composition of the present invention can be prepared according to the usual preparation method of probiotic composition, usually, the composition of the present invention can be in the form of culture suspension or dry powder. And, in the effective dose of the above-mentioned Lactobacillus fermentum PL9988 strain or its culture solution as the main component, one or more than two kinds of pharmaceutically acceptable common carriers or one or more than two kinds of additives can be selected. Compositions prepared in usual dosage forms.

[0053]Carriers can be selected from diluents, lubricants, binders, disintegrants, sweeteners, stabilizers, and preservatives. One or more of them can be used. As an additive, it can be selected from flavors, vitamins, and antioxidants. Use one or more than two.

[0054] In the present invention, all pharmaceutically acceptable carriers and additives can be used as carriers or additives, specifically, as diluents, lactose (lactose mo...

Embodiment 1

[0083] Example 1. Isolation of bacterial strains

[0084] Fecal samples of 101 healthy adults with regular and smooth defecation were received from 8 villages in Jeollabuk-do, the so-called longevity belt of Korea, and arrived at the laboratory within 6 hours in a refrigerated state. After diluting the provided stool samples with sterilized saline, they were diluted in DeManRogosa (MRS, Difco, Becton Dickinson, Sparks, MD, USA) culture medium and smeared to incubate in a 37°C incubator for 48 hours. Colonies exhibiting the form of lactic acid bacteria were collected to isolate individual colonies (Lee, HM, LeeY, 2008, Letters in Applied Microbiology 46, 676-681), and when colonies of the same form were observed in the same individual, a maximum of two were analyzed to isolate strains. The isolated above-mentioned strains were screened for negative reaction strains by catalase reaction test, and then Gram staining was performed to isolate both Gram-positive strains and rod-form...

Embodiment 2

[0085] Example 2. Identification of bacterial strains

[0086] In order to identify the strain isolated in Example 1 above, 16S ribosomal ribonucleic acid gene base sequence analysis was performed.

[0087] Specifically, the base sequence uses EzTaxon-eserver ( http: / / eztaxon- e.ezbiocloud.net / ; Kim et al., 2012) and the online BLAST algorithm mat the National Center for Biotechnology Information Webserver ( http: / / www.ncbi.nlm.nih.gov ) to identify the strains, and a molecular phylogenetic analysis based on the 16S ribosomal ribonucleic acid base sequence.

[0088] Ultimately, as figure 1 As shown, it is confirmed as follows: the Lactobacillus fermentum PL9988 strain of the present invention has a 16S ribosomal ribonucleic acid base sequence recorded in sequence 1, and presents 99.42% of the standard bacterial strain CECT562 (Lactobacillus fermentum strain CECT562) of Lactobacillus fermentum Novel strains with homology of 16S ribosomal ribonucleic acid ( figure 1 ). Mo...

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Abstract

The invention relates to a novel strain, Lactobacillus fermentum strain, and a use thereof. Specifically, novel Lactobacillus fermentum PL9988, novel Lactobacillus fermentum PL9037, novel Lactobacillus fermentum PL9038, novel Lactobacillus fermentum PL9039 and novel Lactobacillus fermentum PL9040 strains, isolated from adults in a longevity village, and culture fluids thereof: have no antibiotic resistance transfer; have excellent intestinal cell adhesiveness, acid resistance and bile resistance; inhibit harmful pathogenic organisms in intestines; and have an immunity-enhancing effect, an endotoxin shock suppression effect and an antioxidant effect. In particular, Lactobacillus fermentum PL9988 strain significantly satisfies all of the above-mentioned conditions as a probiotic, and thus can be effectively used as a probiotic for intestinal health and defecation improvement and enhancement, or as a health food.

Description

technical field [0001] The invention relates to a novel strain of Lactobacillus fermentum isolated from adults in Changshou Village, which helps to improve and enhance defecation activity, and the probiotic use of the strain. Background technique [0002] The importance of the relationship between the diet a person eats and health has been known since ancient times, and in particular, it is known that the distribution of intestinal microorganisms differs depending on the diet a person eats. Intestinal microbes are closely related to human health and disease, thereby determining the immunity of the intestine (Round JL, Mazmanian SK. 2009. Teggut microbiotashapes in testinal immune responses during health and disease, Nature Reviews Immunology 9, 313-323). Published research results to determine whether obesity (TurnbaughPJ, et al., Gordon JI. 2006. Theobesity-associated gut microbiome with increased capacity for energy harvest. Natyre 444, 1027-1031) etc. A person's intestina...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23L33/135A61P31/00A61P39/06A61P37/04C12R1/225
CPCA61K35/74A61K35/747C12N1/20A23L33/135A23G1/42A23G4/12A23G9/36A61K2035/115A61P31/00A61P37/04A61P39/06Y02A50/30C12R2001/225C12N1/205A23V2400/143A61K35/744A23V2002/00
Inventor 李莲姬朴钟洙白璟洙安基铉申恩舟洪贤珍李学美李旻英申铉靖赵永勳
Owner PL BIO CO LTD
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