Vaccine diluent, and preparation method and application thereof
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A diluent and vaccine technology, applied in the field of immunology, can solve the problems of adverse reactions, immunization failure, unsatisfactory immunization effect, etc., and achieve the effect of improving the immunization effect
Active Publication Date: 2019-04-16
CHINA ANIMAL HUSBANDRY IND
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Problems solved by technology
Due to differences in manufacturers, immune effects, etc., in the actual application of vaccines, the requirements for dilution times, dilution methods, and diluents are also very different. If you choose at will, not only cannot achieve a good dilution effect, There may also be adverse reactions
[0005] At present, when animal vaccines are diluted, they are often inoculated with deionized water, physiological saline or PBS buffer solution to inoculate the animals after dilution, which often leads to unsatisfactory immune effect or immune failure.
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Embodiment 1
[0045] Raw materials and mass percentages are as follows:
[0046] 1. 90% aqueous solution for injection;
[0047] 2. Polymer combination:
[0048] Cyclodextrin 4%;
[0049] Chitosan 4%;
[0050] Macrogol 4000 2%.
[0051] Preparation:
[0052]Weigh the combination of the above-mentioned aqueous solution for injection and polymer into the bottom of the stainless steel high-pressure reactor, put in a magnet, and after sealing, put the reactor into a water bath with magnetic stirring, set the temperature of the water bath to 32 to 50°C, and turn on the single cylinder Fill the syringe pump with carbon dioxide to the required pressure of 7.3 to 15 MPa for the experiment, stop punching, close the reactor valve, keep the internal pressure constant, and set the stirring rate at 200 to 2000 rpm. Apply supercritical carbon dioxide to treat and react at a constant temperature in a water bath for 10 to 120 minutes, release the pressure, open the reactor, and take out to obtain the ...
Embodiment 2
[0054] The difference between this embodiment and embodiment 1 is:
[0055] Raw materials and mass percentages are as follows:
[0056] 1. 90% aqueous solution for injection;
[0057] 2. Polymer combination:
[0058] Polycaprolactone 3%;
[0059] Polyfumarate-Sebacic Acid 3%;
[0060] Water soluble starch 4%.
Embodiment 3
[0062] The difference between this embodiment and embodiment 1 is:
[0063] Raw materials and mass percentages are as follows:
[0064] 1. 90% aqueous solution for injection;
[0065] 2. Polymer combination:
[0066]
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Abstract
The invention relates to the field of immunology, and in particular, relates to a vaccine diluent, wherein the vaccine diluent comprises the raw materials: water for injection, a polymer, and an immunoenhancer optionally added. The preparation method of the vaccine diluent comprises the steps: weighing the raw materials, putting to the bottom of a stainless steel high-pressure reactor, putting a magneton, sealing, and putting the reactor into a water bath with magnetic stirring; setting the water bath temperature, starting up a single-cylinder injection pump, charging carbon dioxide to the pressure required in an experiment, stopping stamping, closing a reactor valve, keeping the internal pressure unchanged, and setting the stirring rate; and after placing at constant temperature in waterbath for a certain time and reaction is performed, relieving the pressure, and thus obtaining the vaccine diluent. The diluent combination after supercritical carbon dioxide treatment is easy for industrialized production of vaccines, and is safe and effective. The prepared diluent can be applied not only to an antigen of a live vaccine, but also to an antigen of an inactivated vaccine or an antigen of a synthetic peptide vaccine.
Description
technical field [0001] The invention relates to the field of immunology, in particular to a vaccine diluent. Background technique [0002] Vaccine diluent refers to the reagent used for immunization after the vaccine is diluted. [0003] The role of the vaccine diluent is very important, not only to dilute the vaccine, but also to protect the vaccine and improve the immune effect of the vaccine. [0004] The type, source and production process of the vaccine will have a certain impact on the choice of diluent. Due to differences in manufacturers, immune effects, etc., in the actual application of vaccines, the requirements for dilution times, dilution methods, and diluents are also very different. If you choose at will, not only cannot achieve a good dilution effect, Adverse reactions may also occur. [0005] At present, when animal vaccines are diluted, deionized water or physiological saline or PBS buffer solution is often used to dilute the vaccine and inoculate the an...
Claims
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Application Information
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