Vaccine diluent, and preparation method and application thereof

Abstract

The invention relates to the field of immunology, and in particular, relates to a vaccine diluent, wherein the vaccine diluent comprises the raw materials: water for injection, a polymer, and an immunoenhancer optionally added. The preparation method of the vaccine diluent comprises the steps: weighing the raw materials, putting to the bottom of a stainless steel high-pressure reactor, putting a magneton, sealing, and putting the reactor into a water bath with magnetic stirring; setting the water bath temperature, starting up a single-cylinder injection pump, charging carbon dioxide to the pressure required in an experiment, stopping stamping, closing a reactor valve, keeping the internal pressure unchanged, and setting the stirring rate; and after placing at constant temperature in waterbath for a certain time and reaction is performed, relieving the pressure, and thus obtaining the vaccine diluent. The diluent combination after supercritical carbon dioxide treatment is easy for industrialized production of vaccines, and is safe and effective. The prepared diluent can be applied not only to an antigen of a live vaccine, but also to an antigen of an inactivated vaccine or an antigen of a synthetic peptide vaccine.

Description

technical field [0001] The invention relates to the field of immunology, in particular to a vaccine diluent. Background technique [0002] Vaccine diluent refers to the reagent used for immunization after the vaccine is diluted. [0003] The role of the vaccine diluent is very important, not only to dilute the vaccine, but also to protect the vaccine and improve the immune effect of the vaccine. [0004] The type, source and production process of the vaccine will have a certain impact on the choice of diluent. Due to differences in manufacturers, immune effects, etc., in the actual application of vaccines, the requirements for dilution times, dilution methods, and diluents are also very different. If you choose at will, not only cannot achieve a good dilution effect, Adverse reactions may also occur. [0005] At present, when animal vaccines are diluted, deionized water or physiological saline or PBS buffer solution is often used to dilute the vaccine and inoculate the an...

AI Technical Summary

Problems solved by technology

Due to differences in manufacturers, immune effects, etc., in the actual application of vaccines, the requirements for dilution times, dilution methods, and diluents are also very different. If you choose at will, not only cannot achieve a good dilution effect, There may also be adverse reactions

[0005] At present, when animal vaccines are diluted, they are often inoculated with deionized water, physiological saline or PBS buffer solution to inoculate the animals after dilution, which often leads to unsatisfactory immune effect or immune failure.

Images