Application of rps9 protein in prediction of good response to superovulation in cynomolgus monkeys

A technology of superovulation and cynomolgus monkeys, which is applied in the field of superovulation and can solve the problems that the interaction is not yet clear

Active Publication Date: 2022-04-26
SOUTH CHINA AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, the research on the relationship between RPS9 protein and superovulation has not been reported, and the interaction between RPS9 and ovary, oocyte, etc. in the process of superovulation is not yet clear

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  • Application of rps9 protein in prediction of good response to superovulation in cynomolgus monkeys
  • Application of rps9 protein in prediction of good response to superovulation in cynomolgus monkeys

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Embodiment Construction

[0023] Specific embodiments of the present invention will be further described below. It should be noted here that the descriptions of these embodiments are used to help understand the present invention, but are not intended to limit the present invention. In addition, the technical features involved in the various embodiments of the present invention described below may be combined with each other as long as they do not constitute a conflict with each other.

[0024] The test methods used in the following experimental examples are conventional methods unless otherwise specified; the materials and reagents used are commercially available reagents and materials unless otherwise specified.

[0025] 1. Superovulation experiment process

[0026] Observe the physiological state of adult female cynomolgus monkeys in the target monkey group every day, and register the cage number and monkey number on the first day when menstruation occurs. On the 1st to 4th days of menstruation, ve...

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Abstract

The invention relates to the application of RPS9 protein in the prediction of good response to superovulation in cynomolgus monkeys, belonging to the technical field of superovulation. The method of proteomics is used to analyze the first day of superovulation success group and the fifth day of superovulation success group. , Superovulation failure group on day 1 and superovulation failure group on day 5 for bioinformatics analysis to obtain the differential protein among the groups. From the study of protein level, the changes of protein expression revealed in the blood of superovulated monkeys were explored. In superovulation in cynomolgus monkeys, RPS9 protein was expressed on day 5 in the superovulation success group, but not in the superovulation failure group on day 5. It is suggested that the down-regulation of PRS9 expression in the superovulation failure group affects the activation of MAPK signaling pathway and NK-κB pathway, thereby leading to cell apoptosis, cell cycle arrest and inhibition of proliferation. The down-regulation of PRS9 expression may lead to the activation of p53, resulting in the inhibition of cell proliferation, thereby affecting the formation of follicles and leading to the failure of superovulation response. Therefore, RPS9 protein has become a new molecule to predict the good response to superovulation in cynomolgus monkeys, and has practical application value.

Description

technical field [0001] The invention relates to the technical field of superovulation, and more specifically, relates to the application of RPS9 protein in predicting good response to superovulation in cynomolgus monkeys. Background technique [0002] Superovulation refers to the use of exogenous hormones to stimulate the ovaries of female animals, to promote the simultaneous growth and development of multiple follicles in the ovary, and finally to obtain multiple mature oocytes. In cynomolgus monkeys, only one dominant follicle matures and ovulates during the natural menstrual cycle. If the superovulation technique is used during the menstrual period, the number of oocytes per ovulation can be increased from 1 to 10-20 or even more. Mature oocytes are important materials for research on human diseases, genetic engineering and embryo engineering. However, the number of qualified oocytes obtained through natural cycle is extremely small, which is far from meeting the experi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/68
CPCG01N33/6893G01N2333/47G01N2800/36
Inventor 杨世华侯润杰赵鑫源
Owner SOUTH CHINA AGRI UNIV
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