2 results about "Ammonium bisulfite" patented technology

The application relates to a sample processing method for cervical exfoliative cell PCR detection, which adopts a sample processing reagent, including a processing liquid, a protection liquid, a conversion liquid, a first purification liquid, a second purification liquid, a third purification liquid and a collection liquid. The processing liquid contains 0.1-0.5N sodium hydroxide and 4-8mol / L urea; the protection liquid contains 1-8mM guanidine isothiocyanate, 0.1-5% SDS, 5-150mM Tris-Hcl and 5-100mM EDTA; and the conversion liquid contains 100-500mM sodium bisulfite, 50-250mM ammonium sulfite, 2-15M ammonium bisulfite, 0.1-3M tetrahydrofurfuryl alcohol, 1-5mM TEPA and 0.1-2M magnesium sulfite. The sample is lysed and protected by the processing liquid and the protection liquid, direct conversion and detection are facilitated, processing time and steps are saved, DNA loss is reduced, cost is saved, the accuracy and reliability of detection results are ensured.

This application relates to the field of monolithic refractory materials, specifically disclosing a self-flowing, densified, cement-free refractory castable and its preparation process. The self-flowing, densified, cement-free refractory castable includes aggregates, binders, admixtures, a modified water-reducing agent, and water. The preparation method of the modified water-reducing agent includes the following steps: adding an aqueous solution of methacryloyloxyethyl phosphate, methoxy polyethylene glycol acrylate, acrylic acid, methacryloyloxypropyltrimethoxysilane, and mercaptoacetic acid to water to obtain solution A; adding ammonium persulfate and ammonium bisulfite to water and mixing thoroughly to obtain solution B; adding water to a reaction vessel, heating, and simultaneously adding solutions A and B dropwise to the reaction vessel; after the addition is complete, maintaining the temperature to continue the reaction, cooling, and adjusting the pH with triethanolamine to obtain the modified water-reducing agent. The refractory castable in this application has excellent self-flowing molding, a highly dense structure, and high-temperature resistance and slag corrosion resistance.