7 results about "Ammonium bisulfite" patented technology

The present application relates to a high concentration of ammonium bisulfite preservation solution, the preservation solution components include ammonium bisulfite and ethanol, the concentration of ammonium bisulfite is about 13 mol / L to about 15 mol / L, the concentration of ethanol is about 0.3 mol / L to about 1.5 mol / L. The present application also provides a method and kit for preserving high concentration of ammonium bisulfite solution.

A process for producing ammonium sulfite and ammonium bisulfite from an ammonia gas stream. The process involves injected sulfur dioxide into a circulating liquid stream to an optimal pH that captures gaseous ammonia from a gas stream. The captured ammonia reacts with sulfur dioxide and water to form the desired products.

The application relates to a sample processing method for cervical exfoliative cell PCR detection, which adopts a sample processing reagent, including a processing liquid, a protection liquid, a conversion liquid, a first purification liquid, a second purification liquid, a third purification liquid and a collection liquid. The processing liquid contains 0.1-0.5N sodium hydroxide and 4-8mol / L urea; the protection liquid contains 1-8mM guanidine isothiocyanate, 0.1-5% SDS, 5-150mM Tris-Hcl and 5-100mM EDTA; and the conversion liquid contains 100-500mM sodium bisulfite, 50-250mM ammonium sulfite, 2-15M ammonium bisulfite, 0.1-3M tetrahydrofurfuryl alcohol, 1-5mM TEPA and 0.1-2M magnesium sulfite. The sample is lysed and protected by the processing liquid and the protection liquid, direct conversion and detection are facilitated, processing time and steps are saved, DNA loss is reduced, cost is saved, the accuracy and reliability of detection results are ensured.

This application relates to the field of monolithic refractory materials, specifically disclosing a self-flowing, densified, cement-free refractory castable and its preparation process. The self-flowing, densified, cement-free refractory castable includes aggregates, binders, admixtures, a modified water-reducing agent, and water. The preparation method of the modified water-reducing agent includes the following steps: adding an aqueous solution of methacryloyloxyethyl phosphate, methoxy polyethylene glycol acrylate, acrylic acid, methacryloyloxypropyltrimethoxysilane, and mercaptoacetic acid to water to obtain solution A; adding ammonium persulfate and ammonium bisulfite to water and mixing thoroughly to obtain solution B; adding water to a reaction vessel, heating, and simultaneously adding solutions A and B dropwise to the reaction vessel; after the addition is complete, maintaining the temperature to continue the reaction, cooling, and adjusting the pH with triethanolamine to obtain the modified water-reducing agent. The refractory castable in this application has excellent self-flowing molding, a highly dense structure, and high-temperature resistance and slag corrosion resistance.

The invention relates to the technical field of concrete admixtures and discloses a machine-made sand concrete mixture and a workability regulator, comprising diphenolic acid, an alcohol amine, 2-methyl-2-propylene-1-ol, trifluoromethanesulfonic acid, an unsaturated carboxylic acid ester, 2-acrylamido-2-methylpropanesulfonic acid, ammonium bisulfite, ammonium persulfate and deionized water. The machine-made sand concrete mixture and the workability regulator provided by the invention have good water solubility and good compatibility with a polycarboxylate water-reducing agent. After being added to machine-made sand concrete, the concrete mixture has good cohesion and water retention, and has a good slurrying effect on machine-made sand and gravel. The workability of the concrete mixture is significantly improved, bleeding is reduced, and the slump loss of concrete over time is reduced. The machine-made sand concrete regulator has good adaptability to slump retention over time for different machine-made sand materials of concrete, in particular, machine-made sand containing mud and powder. In addition, the machine-made sand concrete regulator provided by the invention is green and environmentally friendly, has a low production cost, and is easy to promote and apply.

The invention discloses a green surfactant and a preparation method thereof, and belongs to the technical field of surfactants, the preparation method comprises the following steps: adding raw materials butenedioic acid ester and ammonium hydrogen sulfite and a catalyst nano H-Beta molecular sieve into a high-pressure reaction kettle, and introducing carbon dioxide for emptying; the temperature of the reaction kettle is set to be 50-70 DEG C, then carbon dioxide is introduced, the pressure of the reaction kettle is controlled to be 7-9 MPa, a reaction is carried out for 10-30 min, then filtering is carried out, and the obtained filtrate is the ammonium sulfosuccinate dialkyl ester surfactant. According to the preparation method, butenedioic acid ester and ammonium hydrogen sulfite are creatively adopted as raw materials, a nano H-Beta molecular sieve is adopted as a solid acid catalyst, supercritical carbon dioxide is adopted as a solvent, and the ammonium sulfosuccinate dialkyl ester surfactant is prepared through an addition reaction; a melting reaction technology and a supercritical ethanol crystallization reaction technology are introduced into the preparation of the nano H-Beta molecular sieve for the first time.

The invention discloses a kit for carrying out sulfite conversion on nucleic acid. The invention provides a reagent for carrying out sulfite conversion on nucleic acid. The reagent comprises sulfite conversion liquid; the sulfite conversion liquid contains sodium metabisulfite with the final concentration of 0.4-0.8 g / L and ammonium hydrogen sulfite with the mass percent of 10%-20%; the pH value of the sulfite conversion liquid is 5-6. Sodium metabisulfite and ammonium bisulfite are selected as sulfite, the cost is low, the research and development cost of an enterprise can be effectively reduced, large-scale industrial production is promoted, and the method is more suitable for the development requirement of the market. Besides, the temperature of the conversion procedure step in the kit using method is adjusted, so that the whole conversion reaction time is only 70 minutes, the preparation efficiency is high, the preparation time is shorter, and the product is long in preservation time, low in price and stable in supply.