4 results about "Ammonium bisulfite" patented technology

The present application relates to a high concentration of ammonium bisulfite preservation solution, the preservation solution components include ammonium bisulfite and ethanol, the concentration of ammonium bisulfite is about 13 mol / L to about 15 mol / L, the concentration of ethanol is about 0.3 mol / L to about 1.5 mol / L. The present application also provides a method and kit for preserving high concentration of ammonium bisulfite solution.

The application relates to a sample processing method for cervical exfoliative cell PCR detection, which adopts a sample processing reagent, including a processing liquid, a protection liquid, a conversion liquid, a first purification liquid, a second purification liquid, a third purification liquid and a collection liquid. The processing liquid contains 0.1-0.5N sodium hydroxide and 4-8mol / L urea; the protection liquid contains 1-8mM guanidine isothiocyanate, 0.1-5% SDS, 5-150mM Tris-Hcl and 5-100mM EDTA; and the conversion liquid contains 100-500mM sodium bisulfite, 50-250mM ammonium sulfite, 2-15M ammonium bisulfite, 0.1-3M tetrahydrofurfuryl alcohol, 1-5mM TEPA and 0.1-2M magnesium sulfite. The sample is lysed and protected by the processing liquid and the protection liquid, direct conversion and detection are facilitated, processing time and steps are saved, DNA loss is reduced, cost is saved, the accuracy and reliability of detection results are ensured.

This application relates to the field of monolithic refractory materials, specifically disclosing a self-flowing, densified, cement-free refractory castable and its preparation process. The self-flowing, densified, cement-free refractory castable includes aggregates, binders, admixtures, a modified water-reducing agent, and water. The preparation method of the modified water-reducing agent includes the following steps: adding an aqueous solution of methacryloyloxyethyl phosphate, methoxy polyethylene glycol acrylate, acrylic acid, methacryloyloxypropyltrimethoxysilane, and mercaptoacetic acid to water to obtain solution A; adding ammonium persulfate and ammonium bisulfite to water and mixing thoroughly to obtain solution B; adding water to a reaction vessel, heating, and simultaneously adding solutions A and B dropwise to the reaction vessel; after the addition is complete, maintaining the temperature to continue the reaction, cooling, and adjusting the pH with triethanolamine to obtain the modified water-reducing agent. The refractory castable in this application has excellent self-flowing molding, a highly dense structure, and high-temperature resistance and slag corrosion resistance.

The invention discloses a kit for carrying out sulfite conversion on nucleic acid. The invention provides a reagent for carrying out sulfite conversion on nucleic acid. The reagent comprises sulfite conversion liquid; the sulfite conversion liquid contains sodium metabisulfite with the final concentration of 0.4-0.8 g / L and ammonium hydrogen sulfite with the mass percent of 10%-20%; the pH value of the sulfite conversion liquid is 5-6. Sodium metabisulfite and ammonium bisulfite are selected as sulfite, the cost is low, the research and development cost of an enterprise can be effectively reduced, large-scale industrial production is promoted, and the method is more suitable for the development requirement of the market. Besides, the temperature of the conversion procedure step in the kit using method is adjusted, so that the whole conversion reaction time is only 70 minutes, the preparation efficiency is high, the preparation time is shorter, and the product is long in preservation time, low in price and stable in supply.