Application of MxA and SAA in preparation of diagnostic reagent for discriminating children viral influenza

A technology for viral influenza and diagnostic reagents, applied in disease diagnosis, biological testing, material testing, etc., can solve problems such as short half-life of type I IFNs and no diagnostic efficacy, eliminate false positive results, and improve diagnostic sensitivity. Effects with high specificity, specificity and sensitivity

Active Publication Date: 2021-12-31
南京柯瑞生物医疗科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although sometimes used as biomarkers for viruses, type I IFNs have a short half-life in body fluids and have not shown good diagnostic performance

Method used

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  • Application of MxA and SAA in preparation of diagnostic reagent for discriminating children viral influenza
  • Application of MxA and SAA in preparation of diagnostic reagent for discriminating children viral influenza
  • Application of MxA and SAA in preparation of diagnostic reagent for discriminating children viral influenza

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Experimental program
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Effect test

Embodiment 1

[0025] In the present invention, the application of a kind of MxA and SAA or its binding antibody or fragment in the preparation of a diagnostic reagent for screening viral influenza and non-influenza in children, said diagnostic reagent detects the content of MxA and SAA in individual samples and compares with the normal level In contrast, childhood viral influenza was diagnosed when MxA and SAA levels in individual samples exceeded normal levels.

[0026] A product for diagnosing viral influenza in a child individual who has symptoms of influenza. The diagnostic product contains a diagnostic reagent, which contains a binding antibody or binding antibody or fragment of MxA and SAA at the same time, and binds to MxA and SAA in the individual sample through the binding antibody or fragment to detect in the individual sample The MxA and SAA contents were used for diagnosis.

[0027] An application, method and product of MxA and SAA or its binding antibody or fragment in the pre...

Embodiment 2

[0038] In the present invention, a preparation method of a novel MxA and SAA duplex colloidal gold rapid diagnostic kit includes the purification of MxA, the preparation of MxA monoclonal antibody and the development of MxA / SAA, MxA, SAA and CRP rapid detection kit.

[0039] 1. Purification of MxA

[0040] Purify the MxA and MXB mixture extracted from lymphocytes by ion-exchange chromatography according to the laboratory's operating procedures. We used Pharmacia MonoQ5 / 50 ion-exchange columns from Amersham Biosciences, and the eluent gradient was 0.15 to 0.3mol / L NaCl and 50 mmol / L sodium acetate. Purified MxA was further confirmed by tandem mass spectrometry and western-blot verification.

[0041] 2. Preparation of MxA monoclonal antibody

[0042] For antigen pretreatment, take an appropriate amount of antigen and mix it with an equal volume of Freund's complete adjuvant, and use a syringe to suck and emulsify into a water-in-oil state to immunize mice;

[0043] Immunization...

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Abstract

The invention relates to application of MxA and SAA or a binding antibody or fragment thereof in preparation of a diagnostic kit for discriminating viral influenza and non-influenza of children. An immunofluorescence method is adopted to monitor influenza-like symptom patients including influenza A, influenza B, influenza C, parainfluenza virus 1, parainfluenza virus 2, parainfluenza virus 3, adenovirus and respiratory syncytial virus infection. The kit can effectively eliminate false positive results in diagnosis, has high specificity and sensitivity, is simple to operate, has been clinically verified, is suitable for further clinical popularization, not only improves the diagnosis sensitivity of children influenza, but also detects non-specific inflammatory factors SAA while detecting MxA. Also, the kit has the advantage that bacterial infection or virus infection can be distinguished.

Description

technical field [0001] The invention belongs to the technical field of medical biological diagnosis, and is especially suitable for the preparation of dual detection diagnostic reagents for screening viral influenza in children by MxA and SAA or their binding antibodies or fragments. Background technique [0002] Influenza is a common infectious disease caused by influenza virus. Because the surface ceramidase and hemagglutinin of influenza virus are prone to mutation, new influenza virus infection may occur even in people who have been vaccinated against influenza. Currently, viral influenza in children The inspection methods have problems such as high specimen requirements, low sensitivity, and long monitoring time. When the laboratory diagnosis results are not reported, the patient's symptoms, signs, and white blood cell count and other parameters are used to refer to whether there is viral influenza, and the probability of missed diagnosis and misdiagnosis occurs. higher...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/577G01N33/543
CPCG01N33/6893G01N33/54313G01N33/577G01N2800/26Y02A50/30
Inventor 杨轶轩
Owner 南京柯瑞生物医疗科技有限公司
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