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Cell wax block preparation kit and preparation method thereof

A technology of cell wax blocks and kits, which is applied in the field of analysis, can solve the problems of cumbersome procedures and long time, and achieve the effects of great application value, less samples, and reduced loss

Active Publication Date: 2017-05-31
济南天灿医疗科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Aiming at the problems of cumbersome preparation process and long time in the prior art, the present invention provides a rapid and effective preparation kit for cell paraffin blocks

Method used

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  • Cell wax block preparation kit and preparation method thereof
  • Cell wax block preparation kit and preparation method thereof
  • Cell wax block preparation kit and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0029] A cell wax block preparation kit, including reagent A, reagent B, disposable dropper and dehydration box;

[0030] The reagent A is a polyanionic cellulose aqueous solution with a mass fraction of 0.4% (viscosity is 1400mpas, degree of substitution is 0.8), and the reagent B is a mixture of ethanol and acetone (the volume ratio of ethanol to acetone is 85:15) ; The disposable dropper is 1-2ml; The specification of the embedding cassette is 37×25×6mm;

[0031] The method for preparing the cell wax block by the cell wax block preparation kit comprises the following steps:

[0032] (1) Drop reagent A into the centrifuged and concentrated cell solution at a volume ratio of 1:1, shake and mix, add reagent B at 1:10, shake gently, and then centrifuge to precipitate or take out the precipitate directly;

[0033] (2) According to histopathology, the steps of fixation, dehydration and other steps can be followed by paraffin embedding.

Embodiment 2

[0035] A cell wax block preparation kit, comprising reagent A, reagent B, a disposable dropper and an embedding box;

[0036] The reagent A is an aqueous solution of sodium polyanion cellulose with a mass concentration of 1.2% (viscosity is 1500 mpas, degree of substitution is 0.9), and the reagent B is a mixture of ethanol and acetone (the volume ratio of ethanol and acetone is 95:5 ); the disposable dropper is 1ml; the specification of the embedding cassette is 37×25×6mm;

[0037] The method for preparing the cell wax block by the cell wax block preparation kit comprises the following steps:

[0038] (1) Drop reagent A into the centrifuged concentrated cell solution at a volume ratio of 1:1, shake and mix well, add reagent B at 1:15, shake gently and mix well, then centrifuge to precipitate or take out the precipitate directly;

[0039] (2) According to histopathology, the steps of fixation, dehydration and other steps can be followed by paraffin embedding.

Embodiment 3

[0041] A cell wax block preparation kit, comprising reagent A, reagent B, a disposable dropper and an embedding box;

[0042] The reagent A is an aqueous solution of polyanionic cellulose potassium with a mass concentration of 0.8% (viscosity is 1500 mpas, and the degree of substitution is 0.9), the reagent B is ethanol; the disposable dropper is 2ml; the embedding box The specification is 37×25×6mm;

[0043] The preparation method of the cell wax block is the same as that in Example 2.

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Abstract

The invention belongs to the technical field of analysis, and particularly relates to a cell wax block preparation kit and a preparation method thereof. The cell wax block preparation kit comprises a reagent A and a reagent B; and the reagent A is an aqueous solution prepared from any one of polyanionic celluloses, polyanionic cellulose sodium, polyanionic cellulose potassium, carboxymethylcellulose, sodium carboxymethylcellulose and potassium carboxymethylcellulose, and the reagent B is an organic solvent. The reagent provided by the invention is used for producing a cell wax block, so that fewer samples such as tissue fluid and the like are needed, the success rate is high, no adverse effect on the cell is produced, the loss of the cell is reduced, more cells are maintained, the detection accuracy is improved, the application value for preparing the cell wax block for cell needle aspirations, cutting needle biopsy, body fluid and residual deposits and various micro fragments prepared by other cytology clinically is high, and the daily increasing requirements on the scientific research and clinical pathological diagnosis can be met.

Description

technical field [0001] The invention belongs to the technical field of analysis, and in particular relates to a cell wax block preparation kit and a preparation method thereof. Background technique [0002] With the development of cytology, immunohistochemistry and molecular biology, cell wax block technology is becoming more and more important in the application of cytological diagnosis, prognosis judgment and targeted drug therapy. How to make cell wax block conveniently and quickly is the key to cell Common challenges with wax block technology. [0003] In the prior art, there are probably the following methods for the production of cell paraffin blocks: (1) method of cell aggregation on slides of pleural fluid; (2) method of direct preparation of cell paraffin blocks from fine-needle aspiration fluid; Block preparation method; among the above methods, method (1) and method (2) require a large amount of cells, and the preparation process is cumbersome, and the success ra...

Claims

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Application Information

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IPC IPC(8): G01N1/28G01N1/30G01N1/36
Inventor 张震
Owner 济南天灿医疗科技有限公司
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