Method for rapid screening of parasitic weed sensitive herbicide

Abstract

The invention discloses a method for rapid screening of a parasitic weed sensitive herbicide. The method comprises: taking parasitic weed seeds, conducting surface sterilization, rinsing and air drying; inoculating the seeds to a moisture absorption matrix containing a to-be-screened herbicide, and carrying out pre-culture under closed and dark conditions; transferring the seeds to the moisture absorption matrix containing a seed germination stimulus, and conducting germination test under closed and dark conditions; calculating the seed germination rate, and screening out the parasitic weed sensitive herbicide. The herbicide is directly used in the pre-culture stage of the parasitic weed seeds to simulate the field application and action process of the herbicide. The seed germination stimulus GR24 is employed to replace a host root exudate, thereby eliminating a false positive result caused by germination stimulus type and content differences, and making seeds germinate under laboratory conditions. The control effect of the herbicide on seed germination can be quickly definite by only 2 weeks. The data is true and reliable, and provides reference for field use of the herbicide.

Description

technical field [0001] The invention belongs to the technical field of rapid screening of herbicides, in particular to a method for rapid screening of sensitive herbicides of parasitic weeds. Background technique [0002] Parasitic plants refer to a class of plants that must rob certain nutrients and water from the host to meet their own growth and development needs due to root or leaf degeneration or lack of sufficient chlorophyll. [0003] According to the degree of dependence on the host, parasitic plants can be mainly divided into two types: one type has no leaves or the leaves degenerate into scales, and cannot perform normal photosynthesis. The nutrients required by itself come entirely from the host, which is called a total parasitic plant. , such as Cuscuta chinensis and Orobanche spp., etc.; the other type contains chlorophyll and can carry out normal photosynthesis, but the roots are mostly degenerated, and the vessels are directly connected to the host plant to ab...

AI Technical Summary

Problems solved by technology

[0006] The disadvantage of this method of weeding is that it lacks systematic and in-depth research. Indiscriminate use and abuse of herbicides often occur in agricultural production, causing significant damage to crops and the environment.

At the same time, the field screening of herbicides often has problems such as long period (at least the entire growth cycle of the crop is required), large investment (requires a large number of crops to be planted in a large area), high risk (potential risk of yield loss), and field screening. There are also often uncertain factors, such as differences in seed density and species of parasitic weeds in different fields, differences in resistance of different crops, etc., which will cause false positive results

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