Method for expressing human insulin for reducing blood sugar by transgenic lucid ganoderma

A human insulin, transgenic technology, applied in other methods of inserting foreign genetic materials, gene therapy, pharmaceutical formulations, etc., can solve the problems of skin allergy and neuralgia, gene deletion, insufficient secretion of main and companion insulin, and reduce blood sugar levels. Effect
CN1879891AInactive Publication Date: 2006-12-20林忠平
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
林忠平
Publication Date
2006-12-20
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to an artificial insulinogen analogue gene, while it adds the internal stay sequence of KDEL to accumulate the protein in the internal network to avoid degrading enzyme of cell. And it uses GPD of high level epiphyte to start the target gene and arranged in the carrier transformed with lucid ganoderma; uses electric hitting method or agricillin or PEG medium gene method to transform lucid ganoderma; the ELISA test has proved that the content of artificial insulinogen analogue is 105.0 mug / g-174.8 mug / g of the fresh weight, and at 4.40%-10.40% of soluble protein. And the invention can effectively reduce blood sugar.
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Description

1. Technical field:

[0001] The invention relates to artificially synthesizing human insulin gene and modifying the coding region structure. A promoter construct with strong expression in fungi was chosen. Place in a carrier suitable for transformation of Ganoderma lucidum, and genetically transform Ganoderma lucidum by electric shock method, Agrobacterium-mediated method or PEG-mediated method. Ganoderma lucidum expressing human insulin was obtained, and it was proved effective by oral gavage feeding to hyperglycemic rats, thus providing a method for using transgenic Ganoderma lucidum to lower blood sugar. 2. Background technology:

[0002] 1. Insulin

[0003] Insulin is a small molecular protein secreted by pancreatic β cells, with a molecular weight of about 6000kD. There are two peptide chains in the molecule, called A chain and B chain. The A chain of human insulin has 21 amino acid residues, and the B chain has 30 amino acid residues. The A and B chains are connected...

Claims

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