Full-automatic enzyme-linked immunoassay system

An enzyme-linked immunoassay, fully automatic technology, applied in the field of automatic enzyme-linked immunoassay analyzer, can solve the problem of low processing efficiency and achieve the effect of improving efficiency

Inactive Publication Date: 2006-12-27
YANTAI ADEKANG BIOTECHNOLOGY CO LTD
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Problems solved by technology

[0005] In order to overcome the problem of low processing efficiency of the imported automatic ELISA instrument, the present invention adopts the industrial assembly line ELISA treatment method, and carries out the mechanical structure layout according to the procedures and conditions required by the ELISA; the ELISA liquid The adding unit, constant temperature incubation unit and plate washing unit can complete the tasks independently without delaying each other under the unified control of the computer, and can cooperate in a unified way to complete the reaction process together

Method used

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  • Full-automatic enzyme-linked immunoassay system

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Embodiment

[0015] Example (1)---Enzyme-linked immunological reaction process for the determination of HIV antibody by double-antigen sandwich method

[0016] The reaction steps are as follows: First, add 100 μl of specimen to the microwells of HIV-specific antigen-coated microwell reaction plates (also called microplates), and then incubate at 37 degrees for 60 minutes with a plate washer. Wash the plate 5 times; then add 100 μl of enzyme conjugate to the microwell, incubate for 30 minutes and wash the plate twice; then add 50 μl of substrate buffer and 50 μl of chromogenic solution to the microwell, and incubate at 37 degrees After 10 minutes, add 50 μl of stop solution, and the reaction process ends. Put the microplate plate into the microplate reader for result interpretation.

[0017] In the implementation process, put the microplate plate into the carrying carrier, and then snap the carrying carrier into the track. The computer-controlled reaction process begins. A liquid additio...

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Abstract

The invention relates to a full-automatic enzyme couple immune analyze system, used in ELISA test of antigen and antibody of medical organizations, which can overcome the defects of low efficiency. The invention uses enzyme couple immune processing method of industrial product line, to distribute the structure based on needed program and condition of enzyme couple immune reaction, to realize the liquid adding unit, the constant-temperature cultivate unit and plate washing unit of said reaction, which can independently complete mission and cooperate too. The invention can improve the efficiency 1-10 times than present instruments.

Description

technical field [0001] The invention relates to a fully automatic enzyme-linked immunoassay analyzer. In various medical institutions, it is mainly used as an automatic analysis instrument for enzyme-linked immunoassay detection of antigens and antibodies of various viruses such as hepatitis A, hepatitis B, and AIDS. Background technique [0002] Enzyme-linked immunoassay (ELISA) is a new discipline developed in modern times. It is a classic method to detect various trace antigens and antibodies by using the specificity and catalytic amplification of enzymes. At present, major, medium and small hospitals, blood banks, epidemic prevention stations, scientific research and other departments mainly use this detection method to determine whether a patient is infected with a certain virus or has produced a certain antibody. [0003] ELISA uses 96 empty ELISA plates as a basic processing unit. Depending on the project, the enzyme-linked immunosorbent reaction requires different ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/543B01L7/00
Inventor 刘子忠王勇
Owner YANTAI ADEKANG BIOTECHNOLOGY CO LTD
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