Immunogen of cycloproply thaastral and preparation process thereof

A technology of ciprofloxacin and immunogen, which is applied in the field of enzyme-linked immunosorbent assay of ciprofloxacin

Inactive Publication Date: 2005-01-05
SHANDONG UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

As far as we know, there is no report on the synthesis of the immunogen of ciprofloxacin in the world

Method used

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  • Immunogen of cycloproply thaastral and preparation process thereof
  • Immunogen of cycloproply thaastral and preparation process thereof

Examples

Experimental program
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Embodiment Construction

[0029] (1) Preparation of solution A: In a 50ml circular reaction bottle, add 13.9mg (0.042mmol) of ciprofloxacin (0.042mmol), 19.33mg (0.168mmol) of hydroxysuccinic acid imide (NHS), ethyl [3 -(Dimethylamino)propyl]carbodiimide hydrochloride (EDC.HCl) 48.3mg (0.252mmol), dissolved in 10ml of dimethylformamide (DMF). React for 4 hours. Solution A is obtained. spare.

[0030] (2) Preparation of Solution B: In a 100ml round reaction bottle, add 476mg BSA and 50ml PBS (pH=7.4). Solution B is obtained. spare.

[0031] (3) Preparation of ciprofloxacin immunogen: gradually add solution A to solution B at 20° C. under stirring. React for 4 hours. A ciprofloxacin immunogen solution was obtained. Dialyze against PBS buffer for three days, changing the dialysate every 12 hours. Centrifuge at high speed (20,000g) for 30 minutes. The supernatant was freeze-dried to obtain 412 mg of white solid powder, which was the crude ciprofloxacin immunogen. Sephadex-G75 (Sigma) was used for...

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Abstract

The invention is a Ciprofloxacin immunogen, coupling Ciprofloxacin with bovine serum albumin at room temperature, using PBS buffer liquor to dialyze, freeze-drying supernatant by high-speed centrifugation and obtaining Ciprofloxacin crude product, then using glucose gel chromatography for purifying, freeze-drying to obtain Ciprofloxacin pure product. The research shows it succeeds in synthesizing the immunogen able to be used in preparing Ciprofloxacin specific antibody. It lays the foundation of development of ELISA test method of Ciprofloxacin medicine residues.

Description

Technical field [0001] The invention relates to the field of detection of animal source food drug residues. related to the enzyme-linked detection method. More specifically, it relates to the production technology of detection reagents used in the enzyme-linked immunoassay method for ciprofloxacin. Background technique [0002] The following definitions apply throughout the specification and claims: [0003] BSA = bovine serum albumin (Bovine Serum Albumin) [0004] PBS=Phosphate Buffered Saline (0.01M, [0005] pH=7.4) [0006] Sephadex-G75 = dextran gel [0007] Ciprofloxacin-EDC = active intermediate formed by ciprofloxacin and carbodiimide [0008] Ciprofloxacin (Ciprofloxacin) belongs to the quinolone antibiotics. Because of its broad antibacterial spectrum and strong bactericidal ability, this kind of antibiotics is widely used in livestock and poultry aquaculture after oxytetracycline. Such drugs can effectively prevent and treat bacterial and mycoplasma ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/765G01N33/531
Inventor 郗日沫卢圣欣
Owner SHANDONG UNIV
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