High-efficiency expression of human epidermal growth factor fusion protein and preparation method and application thereof
By introducing glycine and His tags to the C-terminus of hEGF, combined with E. coli expression and nickel column purification, the problems of efficient production and stability of hEGF were solved. Furthermore, the stable delivery and release of drugs were achieved using ROS-responsive hydrogel compositions, expanding its applications in wound healing and cosmetics.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- GUILIN MEDICAL UNIVERSITY
- Filing Date
- 2026-03-30
- Publication Date
- 2026-07-17
AI Technical Summary
Existing technologies struggle to produce human epidermal growth factor (hEGF) efficiently and stably, and its instability in liquid formulations makes sustained release and targeted delivery difficult, limiting its application in wound healing and cosmetics.
By introducing hydrophilic glycine into the C-terminus of hEGF and fusing it with a His tag, an E. coli expression system was used, followed by nickel column affinity chromatography purification, and then combined with a ROS-responsive hydrogel composition to achieve efficient expression and stable delivery.
This study achieved efficient expression and stable purification of hEGF, reduced production costs, and provided application potential in wound healing and skin repair. Furthermore, the hydrogel composition enabled stable drug release and efficient absorption in the wound environment.
Smart Images
Figure CN122404570A_ABST