Mouth wash for promoting postoperative wound healing and preparation method thereof

Abstract

The invention relates to mouthwash for promoting postoperative wound healing and a preparation method thereof, and belongs to the technical field of oral care. The invention discloses mouthwash for promoting postoperative wound healing. Comprising 0.5%-2% of a green tea extract, 0.5%-1.5% of a centella asiatica extract, 0.1%-0.5% of a mint extract, 0.1%-0.5% of a lotus leaf extract, 0.1%-0.5% of a spanishneedles herb extract, 5%-15% of a humectant, 0.1%-1% of arginine, 0.1%-1% of a sweetening agent, 0.001%-0.1% of recombinant human epidermal growth factors and the balance of water. The mouthwash is prepared from natural, safe and non-irritant components, so that oral bacteria can be effectively inhibited, and wound healing can be promoted; stability is good, taste is comfortable, and long-term use by patients is facilitated; the oral care solution is expected to be provided for postoperative patients with better quality and more effective oral care solutions, and wound healing and oral health recovery are assisted.

Description

Technical Field

[0001] The invention belongs to the technical field of oral care, and in particular relates to a mouthwash for promoting postoperative wound healing and a preparation method thereof. Background Art

[0002] With the continuous advancement of medical technology, postoperative care for oral surgeries such as tonsillectomy has attracted considerable attention. The postoperative wound healing process is often accompanied by pain and the risk of infection, which can affect patients' daily activities such as eating and speaking. Mouthwash, a common oral care product, while effective in cleansing the mouth, removing bad breath, and preventing tooth decay, has limited effectiveness in promoting the healing of special wounds such as those caused by tonsillectomy surgeries, making it difficult to meet the specific needs of postoperative wound healing.

[0003] There are a wide variety of mouthwashes available on the market, but their ingredients and functionality present numerous issues. Many mouthwashes contain synthetic flavors and solvents, resulting in a monotonous taste and variable quality. Antibacterial mouthwashes often use chemical antibacterial agents such as triclosan, metronidazole, and chlorhexidine. While these agents have broad-spectrum antibacterial and bactericidal properties, long-term use can easily lead to drug resistance in oral microorganisms and are toxic to humans, making them unsafe. For example, triclosan reacts with chlorinated tap water to form chloroform, which can damage the heart and liver, is mildly teratogenic, and may even induce liver cancer and cause irregular heartbeats. Excessive fluoride intake can affect tooth and bone development, leading to skeletal fluorosis and dental fluorosis. In severe cases, it can even cause acute fluorosis, such as nausea, vomiting, and irregular heartbeats. Some products using plant extracts for antibacterial solutions also suffer from poor taste, bitterness, and astringency, impacting formula stability and functionality. In clinical practice, antibiotic oral care products, including chlorhexidine, hydrogen peroxide, and metronidazole, offer significant antibacterial and sterilization benefits, but are prone to developing drug resistance and are unsuitable for long-term use. Alcohol-based mouthwashes offer effective sterilization but are highly irritating, resulting in a poor user experience and the potential for secondary damage to the oral mucosa.

[0004] Therefore, developing a mouthwash that can specifically promote postoperative wound healing has important clinical significance and market demand. Summary of the Invention

[0005] In order to solve the above technical problems, the present invention provides a mouthwash for promoting postoperative wound healing and a preparation method thereof.

[0006] The first object of the present invention is to provide a mouthwash for promoting postoperative wound healing. The mouthwash for promoting postoperative wound healing comprises, by mass fraction, 0.5%-2% of green tea extract, 0.5%-1.5% of Centella asiatica extract, 0.1%-0.5% of peppermint extract, 0.1%-0.5% of lotus leaf extract, 0.1%-0.5% of Bidens pilosa extract, 5%-15% of moisturizer, 0.1%-1% of arginine, 0.1%-1% of sweetener, 0.001%-0.1% of recombinant human epidermal growth factor, and the balance being water.

[0007] In one embodiment of the present invention, the green tea extract contains active ingredients such as tea polyphenols and catechins, which can scavenge free radicals generated by wounds, alleviate oxidative stress damage, and thus reduce inflammation, creating a favorable microenvironment for wound healing. It also has a certain antibacterial effect, inhibiting the growth of various harmful bacteria in the mouth and reducing the risk of wound infection.

[0008] In one embodiment of the present invention, the active ingredients of the Centella asiatica extract are triterpenoids, including asiaticoside, madecassoside, asiatic acid, and madecassic acid, which can promote granulation tissue proliferation and collagen synthesis, enhance wound tension strength, and accelerate wound contraction and healing. It can also regulate cell proliferation and differentiation, promote angiogenesis, improve blood circulation in wounds, and provide more nutrients and oxygen for wound repair. Furthermore, Centella asiatica extract has significant anti-inflammatory and antibacterial effects, inhibiting the production of inflammatory factors, alleviating wound inflammatory reactions, and preventing bacterial infection.

[0009] In one embodiment of the present invention, the mint extract contains active ingredients such as menthol and menthone, which have cooling, analgesic, swelling-relieving, and anti-inflammatory effects, alleviating pain and swelling in postoperative wounds and improving patient comfort. Furthermore, it possesses certain antibacterial and antiviral properties, inhibiting the growth and reproduction of bacteria and viruses in wounds and preventing infection.

[0010] In one embodiment of the present invention, the lotus leaf extract contains active ingredients such as nuciferine, citric acid, and rutin, which have the effects of clearing away heat and detoxifying, cooling blood and stopping bleeding, and antibacterial and anti-inflammatory effects. It can inhibit the growth and reproduction of bacteria, reduce the chance of wound infection, and at the same time alleviate the inflammatory response of the wound, thereby promoting hemostasis and healing of the wound.

[0011] In one embodiment of the present invention, the Bidens pilosa extract contains active ingredients such as Bidens pilosa glycoside, which has antibacterial, anti-inflammatory, and immune-enhancing effects. It can inhibit the activity of various bacteria and viruses, prevent wound infection, reduce inflammatory response, and promote the self-repair ability of wounds by enhancing the body's immunity.

[0012] In one embodiment of the present invention, arginine is an important amino acid that regulates the acid-base balance at the wound site, maintaining an appropriate pH value and creating a favorable microenvironment for wound healing. Furthermore, arginine can serve as a precursor to nitric oxide, promoting its synthesis. Nitric oxide plays an important role in regulating vasodilation, increasing blood circulation at the wound site, and promoting cell proliferation and migration, thereby accelerating wound healing.

[0013] In one embodiment of the present invention, the recombinant human epidermal growth factor is a growth factor that has the ability to strongly promote cell proliferation and differentiation. It can act specifically on cells at the wound site, stimulate the proliferation and migration of epidermal cells, fibroblasts, etc., accelerate the epithelialization process and tissue repair of the wound, and significantly shorten the wound healing time.

[0014] In one embodiment of the present invention, the preparation of the green tea extract, Centella asiatica extract, mint extract, lotus leaf extract or Bidens pilosa extract comprises the following steps: taking green tea, Centella asiatica, mint, lotus leaf or Bidens pilosa and extracting with water 1-3 times, adding 6-15 times the weight of green tea, Centella asiatica, mint, lotus leaf or Bidens pilosa water each time and boiling, filtering the decoction, combining the filtrates, concentrating, precipitating with alcohol, recovering ethanol, and spray drying to obtain the green tea extract, Centella asiatica extract, mint extract, lotus leaf extract or Bidens pilosa extract.

[0015] In one embodiment of the present invention, the moisturizing agent includes glycerol and propylene glycol. First, glycerol has multiple hydroxyl groups (-OH) and can form hydrogen bonds with water molecules, thereby strongly adsorbing moisture in the environment; propylene glycol also contains hydroxyl groups and has good hygroscopicity. After the two are compounded, they can more effectively capture and lock in moisture by virtue of the different numbers and spatial arrangements of hydroxyl groups, thereby synergistically enhancing the moisturizing performance. Secondly, the compounding of the two can adjust the overall hydrophilic-lipophilic balance and reduce irritation to the skin and mucous membranes. Furthermore, the molecular structures of glycerol and propylene glycol are different. After compounding, they can broaden the solubility range, better dissolve other ingredients, and improve the dispersibility of some ingredients that are difficult to dissolve in a single solvent, ensuring that the various ingredients in the mouthwash system are fully and evenly mixed. Finally, when the two are compounded and formed into a film on the surface of the skin or mucous membrane, a more flexible and uniform protective film can be formed. This film can prevent water loss without hindering the breathability of the wound.

[0016] In one embodiment of the present invention, the mass ratio of glycerol to propylene glycol is (5-10):(2-5).

[0017] In one embodiment of the present invention, the sweetener is selected from one or more of xylitol, steviol glycosides, aspartame, maltitol, sorbitol and erythritol.

[0018] In one embodiment of the present invention, the mouthwash for promoting postoperative wound healing further comprises a pH regulator; the pH of the mouthwash is adjusted to 6.0-7.0 to maintain the acid-base balance of the mouthwash and ensure the stability and effectiveness of each component.

[0019] In one embodiment of the present invention, the pH adjuster is selected from one or more of citric acid, malic acid, tartaric acid, acetic acid, lactic acid, fumaric acid, sodium citrate and sodium acetate.

[0020] A second object of the present invention is to provide a method for preparing the mouthwash for promoting postoperative wound healing, comprising the following steps:

[0021] S1. Add green tea extract, Centella asiatica extract, mint extract, lotus leaf extract, Bidens pilosa extract, moisturizer, arginine, and sweetener to water, heat and stir, and obtain a mixed solution;

[0022] S2. Cool the mixed solution described in S1 to 0°C-35°C, add recombinant human epidermal growth factor and stir evenly; then add a pH regulator to adjust the pH to 6.0-7.0, and filter to obtain the mouthwash for promoting postoperative wound healing.

[0023] In one embodiment of the present invention, in S1, the heating and stirring temperature is 40°C-50°C, the rotation speed is 200r / min-400r / min, and the time is 20min-40min.

[0024] In one embodiment of the present invention, in S2, the stirring speed is 100 r / min-200 r / min, and the stirring time is 10 min-15 min.

[0025] The technical solution of the present invention has the following advantages over the prior art:

[0026] (1) The green tea extract and Centella asiatica extract in the mouthwash of the present invention both have antioxidant and anti-inflammatory effects, which can jointly reduce oxidative stress and inflammatory reactions in wounds and accelerate the wound healing process. Furthermore, the antibacterial effect of the green tea extract can make up for the relatively weak link in the antibacterial effect of Centella asiatica extract, more comprehensively preventing wound infection. In addition, the cooling effect of the green tea extract and the cooling components of the peppermint extract work together to enhance the cooling and comfortable feeling of the mouthwash during use, making patients more willing to use it, thereby ensuring treatment compliance and indirectly promoting wound healing.

[0027] (2) The Centella asiatica extract and lotus leaf extract in the mouthwash of the present invention both promote blood circulation and angiogenesis, further enhancing blood supply to the wound site and accelerating wound healing. At the same time, the anti-inflammatory and antibacterial properties of the Centella asiatica extract complement the heat-clearing, detoxifying, antibacterial and anti-inflammatory properties of the lotus leaf extract, effectively controlling wound infection and reducing inflammation. Furthermore, the active ingredients of the Centella asiatica extract can synergize with the antibacterial and anti-inflammatory components of the Bidens pilosa extract, enhancing the antibacterial and anti-inflammatory effects of the mouthwash on wounds and jointly maintaining a favorable wound healing environment.

[0028] (3) The cooling and analgesic effect of the mint extract in the mouthwash of the present invention can relieve the tension and discomfort of the oral mucosa caused by wound pain, while arginine can regulate the pH value of the wound site and maintain a suitable acid-base environment, which is beneficial to the stability and release of the active ingredients in the mint extract, thereby better exerting its medicinal effect.

[0029] (4) The lotus leaf extract and Bidens pilosa L. extract in the mouthwash of the present invention are combined. The heat-clearing and detoxifying effects of the lotus leaf extract and the antibacterial and anti-inflammatory properties of the Bidens pilosa L. extract complement each other, which can more comprehensively eliminate the inflammation and infection factors of the wound and create better conditions for wound healing.

[0030] (5) The Bidens pilosa extract in the mouthwash of the present invention cooperates with recombinant human epidermal growth factor. The Bidens pilosa extract enhances the body's immunity and provides a better internal environment for recombinant human epidermal growth factor to exert its effect, enabling it to more effectively promote cell proliferation and tissue repair at the wound.

[0031] (6) The arginine in the mouthwash of the present invention cooperates with the recombinant human epidermal growth factor. Arginine provides favorable conditions for the recombinant human epidermal growth factor to exert its effect by regulating acid-base balance and promoting blood circulation, thereby enhancing its promoting effect on wound healing.

[0032] (7) The natural, safe, and non-irritating ingredients described in the present invention can effectively inhibit oral bacteria and promote wound healing; they have good stability, a comfortable taste, and are easy for patients to use for a long time; and are expected to provide postoperative patients with better quality and more effective oral care solutions, helping to heal wounds and restore oral health. DETAILED DESCRIPTION

[0033] The present invention will be further described below with reference to specific examples so that those skilled in the art can better understand the present invention and implement it, but the examples are not intended to limit the present invention.

[0034] In the present invention, unless otherwise specified, the preparation of the green tea extract, Centella asiatica extract, mint extract, lotus leaf extract or Bidens pilosa extract used in the embodiments of the present invention comprises the following steps: taking 3 kg of green tea, Centella asiatica, mint, lotus leaf or Bidens pilosa, adding water to extract twice, the first time adding 12 times the weight of the raw materials of water, decocting for 1 hour, and the decoction temperature is 98-102°C; the second time adding 12 times the weight of the medicinal materials of water, decocting for 1 hour, and the decoction temperature is 98-102°C, filtering the decoction, combining the two filtrates, concentrating to 1.13 g / mL, precipitating with alcohol, recovering ethanol, and spray drying to obtain the green tea extract, Centella asiatica extract, mint extract, lotus leaf extract or Bidens pilosa extract.

[0035] In the present invention, unless otherwise specified, the recombinant human epidermal growth factor used in the examples of the present invention was purchased from Sichuan Weikeqi Biotechnology Co., Ltd. with the product number WKQ-0015798.

[0036] Example 1

[0037] The mouthwash for promoting postoperative wound healing of this embodiment comprises, by mass fraction, 1.2% of green tea extract, 1.0% of Centella asiatica extract, 0.3% of peppermint extract, 0.3% of lotus leaf extract, 0.3% of Bidens pilosa extract, 10% of moisturizer, 0.6% of arginine, 0.6% of sweetener, 0.05% of recombinant human epidermal growth factor, and the balance being water;

[0038] The moisturizing agent is obtained by mixing glycerin and propylene glycol in a mass ratio of 7:4;

[0039] The sweetener is xylitol;

[0040] The pH adjusters are citric acid and sodium citrate.

[0041] The preparation of a mouthwash for promoting postoperative wound healing specifically comprises the following steps:

[0042] S1. Add green tea extract, Centella asiatica extract, mint extract, lotus leaf extract, Bidens pilosa extract, moisturizer, arginine, and sweetener to water, heat the mixture to 45° C. in a water bath, and stir at 300 rpm for 30 min to obtain a mixed solution;

[0043] S2. Cool the mixture to room temperature, add recombinant human epidermal growth factor, stir at a speed of 150 r / min for 12 minutes, continue to add pH regulator to adjust the pH to 6.5 and stir evenly, filter through a 0.22 μm microporous filter membrane to obtain a mouthwash that promotes postoperative wound healing.

[0044] Example 2

[0045] The same as Example 1, except that the composition of the mouthwash for promoting postoperative wound healing is as follows:

[0046] Green tea extract 0.5%, Centella asiatica extract 1.5%, peppermint extract 0.1%, lotus leaf extract 0.5%, Bidens pilosa extract 0.1%, moisturizer 15%, arginine 0.1%, sweetener 1%, recombinant human epidermal growth factor 0.001%, balance water.

[0047] Example 3

[0048] The same as Example 1, except that the composition of the mouthwash for promoting postoperative wound healing is as follows:

[0049] Green tea extract 2%, Centella asiatica extract 0.5%, peppermint extract 0.5%, lotus leaf extract 0.1%, Bidens pilosa extract 0.5%, moisturizer 5%, arginine 1%, sweetener 0.1%, recombinant human epidermal growth factor 0.1%, balance water.

[0050] Comparative Example 1

[0051] The method is basically the same as Example 1, except that the composition of the mouthwash for promoting postoperative wound healing is: no green tea extract is added.

[0052] Comparative Example 2

[0053] The method is basically the same as Example 1, except that the composition of the mouthwash for promoting postoperative wound healing is: no mint extract is added.

[0054] Comparative Example 3

[0055] The method is basically the same as Example 1, except that the composition of the mouthwash for promoting postoperative wound healing is: no lotus leaf extract is added.

[0056] Comparative Example 4

[0057] The method is basically the same as Example 1, except that the composition of the mouthwash for promoting postoperative wound healing is: no Bidens pilosa extract is added.

[0058] Comparative Example 5

[0059] The method is basically the same as Example 1, except that the composition of the mouthwash for promoting postoperative wound healing is: arginine is not added.

[0060] Comparative Example 6

[0061] The method is basically the same as Example 1, except that the composition of the mouthwash for promoting postoperative wound healing is: no recombinant human epidermal growth factor is added.

[0062] Comparative Example 7

[0063] The method is basically the same as Example 1, except that the composition of the mouthwash for promoting postoperative wound healing is: propylene glycol is not added.

[0064] Test Example 1 Skin irritation test

[0065] Thirty BALB / c mice were divided into three groups and hair was removed on both sides of the spine. The hair removal range was about 10 cm. 2 . After washing the depilated area for 24 hours, apply 0.5 g of the mouthwash for promoting postoperative wound healing of Examples 1-3 to the depilated area on one side, twice a day, for one week; starting from the second day, trim the hair before application and wash with water to remove the residual test sample. After the experiment, the BALB / c mice were killed, and the heart, liver, kidney and depilated skin were subjected to pathological examination. The results showed that there was no edema, congestion, erythema, bleeding spots and ulcers on the local skin in the depilated area of ​​the BALB / c mouse trunk; and there was no abnormality in the hair color, feeding, limb movement, etc. of the BALB / c mouse; pathological histological examination showed that there was no abnormality in the heart, liver, kidney and depilated skin of the BALB / c mouse. This shows that the mouthwash for promoting postoperative wound healing of the embodiment has no local irritation effect and no systemic toxicity.

[0066] Test Example 2: Stability Experiment

[0067] Referring to the standards of QB T2945-2012 "Oral Cleansing and Care Solutions," the mouthwashes prepared in Examples 1-3 were stored at high temperature (45°C), room temperature, light, refrigeration (4°C), and freezing (-18°C) for three months for stability observation. The mouthwashes prepared in Examples 1-3 for promoting postoperative wound healing were tested for stability in terms of sensory, physicochemical, and microbiological indicators. The results showed that the mouthwashes met the standards for appearance, aroma, pH, and microbiological indicators.

[0068] Test Example 3 Antibacterial Experiment

[0069] Common oral bacteria Staphylococcus aureus ATCC 29213, Escherichia coli ATCC 23502, Candida albicans ATCC 36082, and Streptococcus mutans CCTCC M2017746 were selected as strains. The antibacterial effect test method (suspension quantitative method) for antibacterial daily chemical products in QB / T2738-2012 "Evaluation Method for Antibacterial and Bacteriostatic Effects of Daily Chemical Products" was used for testing. 10g of mouthwash prepared in Examples 1-3 and Comparative Examples 1-7 was completely dissolved in 300mL of distilled water. Based on the habit of mouthwash use, the contact time between the mouthwash and the bacteria was selected to be 30s. The antibacterial rate test results are shown in Table 1:

[0070] Table 1

[0071]

[0072] As shown in Table 1, the mouthwashes of the Examples all achieved an antibacterial rate of 99.99% against Staphylococcus aureus, Escherichia coli, Candida albicans, and Streptococcus mutans, demonstrating their strong antibacterial properties. This is because the mouthwashes contain multiple antibacterial ingredients, such as green tea extract, Centella asiatica extract, peppermint extract, lotus leaf extract, and Bidens pilosa extract. The active substances in these ingredients effectively inhibit bacterial growth and reproduction. The antibacterial rates of the comparative examples, however, were significantly reduced due to the omission of certain ingredients. For example, the omission of green tea extract in Comparative Example 1 significantly reduced the antibacterial rate, as the active ingredients in green tea extract, such as tea polyphenols and catechins, possess significant antibacterial properties. The omission of recombinant human epidermal growth factor in Comparative Example 6 also resulted in a poor antibacterial effect, as recombinant human epidermal growth factor can promote cell proliferation and tissue repair in wounds, indirectly enhancing antibacterial activity. Comparative Example 7, which only lacked propylene glycol, still achieved a high antibacterial rate, but still fell short of the Examples, demonstrating that the synergistic effect of the various ingredients is crucial for the antibacterial effect.

[0073] Test Example 4 Anti-inflammatory Test

[0074] 120 BALB / c mice were randomly divided into 12 groups, namely a model group, a control group (normal saline), and experimental groups of Examples 1-3 and Comparative Examples 1-7. Mouthwash was applied once a day for 5 consecutive days. One hour after the last mouthwash application, 50 μL of xylene was applied to the front and back of the left ear of the mouse. The right ear served as a control. Two hours later, the mouse was killed by cervical dislocation. Both ears were cut along the auricle baseline. A 9mm ear punch was used to punch a round ear piece at the same location. The ears were weighed, and the difference between the left and right ear pieces was used as the swelling degree. The swelling degree test results are shown in Table 2:

[0075] Table 2

[0076] Group Swelling Model Group 18.7±1.8 control group 17.1±3.7 Example 1 9.5±3.2 Example 2 9.8±2.5 Example 3 9.3±2.9 Comparative Example 1 14.2±4.1 Comparative Example 2 13.8±3.9 Comparative Example 3 13.2±3.6 Comparative Example 4 12.9±3.3 Comparative Example 5 14.9±4.5 Comparative Example 6 15.6±5.0 Comparative Example 7 11.4±3.0

[0077] As can be seen from Table 2, the degree of swelling after using the mouthwash of the embodiment is significantly lower than that of the model group and the control group, indicating that it has a significant anti-inflammatory effect. This is mainly because the mouthwash contains a variety of ingredients with anti-inflammatory effects, such as green tea extract and Centella asiatica extract, which can reduce the oxidative stress and inflammatory response of the wound and inhibit the production of inflammatory factors. The swelling of the control group after the lack of certain ingredients is generally higher than that of the embodiment. In particular, the control group 5 lacks arginine and the control group 6 lacks recombinant human epidermal growth factor, and the anti-inflammatory effect is even worse. This is because arginine can regulate the acid-base balance of the wound site, create a good microenvironment for wound healing, and is conducive to the stability and release of anti-inflammatory ingredients; recombinant human epidermal growth factor can promote cell proliferation and tissue repair, and enhance the body's anti-inflammatory ability.

[0078] Test Example 5 Clinical Test

[0079] Test subjects: A total of 100 patients undergoing tonsillectomy were recruited and randomly divided into 10 groups, with 10 patients in each group. Pain indicators were observed and recorded using the Numerical Rating Scale (NRS), which uses a scale of 0-10 to represent pain intensity: 0 is no pain, and 10 is the most severe pain. Patients also rated their own pain intensity based on the scores: 1-3 is mild pain, 4-6 is moderate pain that affects sleep, and 7-10 is severe pain. Oral wound pain scores were collected from volunteers and were assigned a 7-10 scale.

[0080] Test method: Take 10mL of mouthwash, gargle in the mouth for 3 minutes and then spit it out, gargle 5 times a day, and use it continuously for 7 days; observe and record the pain symptoms of the subjects during the daily test, score and observe the wound recovery.

[0081] Efficacy indicators:

[0082] (1) Based on the changes in the subjects' pain sensation before and after the test, the effects were divided into four levels: clinical control, marked effect, effective, and ineffective. Clinical control means the pain disappears (0 points); marked effect means the pain changes from severe (7 points to 10 points) to mild (3 points or less) (3 points); effective means the pain changes from severe (7 points to 10 points) to moderate (4 points to 6 points) (5 points); ineffective means no improvement in pain (7-10 points) (7 points).

[0083] (2) The wound healing condition was examined after 7 days of use. The evaluation criteria were: significant, meaning the wound was completely healed within 7 days of using the mouthwash; improved, meaning the wound improved and the wound surface became smaller within 7 days of using the mouthwash; ineffective, meaning no change was observed in the wound within 7 days of using the mouthwash.

[0084] The test results are shown in Table 3:

[0085] Table 3

[0086]

[0087]

[0088] As can be seen from Table 3, the mouthwash of the embodiment performed well in clinical tests. The average pain score was significantly reduced after 3 days of use, and all wounds were healed after 7 days, with an overall efficacy of 100%. This is attributed to the synergistic effect of the various ingredients in the mouthwash. For example, the cooling and analgesic effects of green tea extract and peppermint extract can relieve pain and swelling of postoperative wounds, and ingredients such as Centella asiatica extract and recombinant human epidermal growth factor can promote wound healing. However, the pain relief and wound healing effects of the comparative examples, which lack certain ingredients, are not as good as those of the embodiment. For example, comparative example 1 lacks green tea extract, and comparative example 2 lacks peppermint extract, and their overall efficacy is only about 70%. This is because the lack of ingredients with pain-relieving and cooling analgesic effects leads to stronger pain sensation in patients and slower wound healing. At the same time, the lack of ingredients that promote wound healing will also affect the recovery process of the wound.

[0089] In summary, the mouthwashes of the Examples all performed well in terms of antibacterial, anti-inflammatory, wound healing, and pain relief, with the various ingredients working in synergy. Comparison of the Examples with the Comparative Examples reveals that the absence of any of the ingredients significantly reduced the efficacy of the mouthwashes, fully demonstrating the scientific nature and rationality of the formulations of the present invention.

[0090] Obviously, the above embodiments are merely examples for clarity of explanation and are not intended to limit the implementation methods. Those skilled in the art will appreciate that other variations or modifications can be made based on the above description. It is not necessary and impossible to enumerate all implementation methods here. Obvious variations or modifications arising therefrom remain within the scope of protection of the present invention.

Claims

1. A mouthwash for promoting postoperative wound healing, characterized in that: The mouthwash for promoting postoperative wound healing comprises, by mass fraction, 0.5%-2% of green tea extract, 0.5%-1.5% of Centella asiatica extract, 0.1%-0.5% of peppermint extract, 0.1%-0.5% of lotus leaf extract, 0.1%-0.5% of Bidens pilosa extract, 5%-15% of moisturizer, 0.1%-1% of arginine, 0.1%-1% of sweetener, 0.001%-0.1% of recombinant human epidermal growth factor, and the balance being water.

2. The mouthwash for promoting postoperative wound healing according to claim 1, characterized in that The preparation of the green tea extract, Centella asiatica extract, mint extract, lotus leaf extract or Bidens pilosa extract comprises the following steps: taking green tea, Centella asiatica, mint, lotus leaf or Bidens pilosa and extracting with water for 1-3 times, adding 6-15 times the weight of green tea, Centella asiatica, mint, lotus leaf or Bidens pilosa water for decoction each time, filtering the decoction, combining the filtrates, concentrating, precipitating with alcohol, recovering ethanol, and spray drying to obtain the green tea extract, Centella asiatica extract, mint extract, lotus leaf extract or Bidens pilosa extract.

3. The mouthwash for promoting postoperative wound healing according to claim 1, characterized in that: The humectants include glycerin and propylene glycol.

4. The mouthwash for promoting postoperative wound healing according to claim 3, characterized in that: The mass ratio of the glycerol to propylene glycol is (5-10):(2-5).

5. The mouthwash for promoting postoperative wound healing according to claim 1, characterized in that: The sweetener is selected from one or more of xylitol, steviol glycosides, aspartame, maltitol, sorbitol and erythritol.

6. The mouthwash for promoting postoperative wound healing according to claim 1, characterized in that: The mouthwash for promoting postoperative wound healing further comprises a pH regulator; the pH of the mouthwash is adjusted to 6.0-7.

0.

7. The mouthwash for promoting postoperative wound healing according to claim 6, characterized in that: The pH regulator is selected from one or more of citric acid, malic acid, tartaric acid, acetic acid, lactic acid, fumaric acid, sodium citrate and sodium acetate.

8. The method for preparing the mouthwash for promoting postoperative wound healing according to any one of claims 1 to 7, wherein: The following steps are involved: S1. Add green tea extract, Centella asiatica extract, mint extract, lotus leaf extract, Bidens pilosa extract, moisturizer, arginine, and sweetener to water, heat and stir, and obtain a mixed solution; S2. Cool the mixed solution described in S1 to 0°C-35°C, add recombinant human epidermal growth factor and stir evenly; then add a pH regulator to adjust the pH to 6.0-7.0, and filter to obtain the mouthwash for promoting postoperative wound healing.

9. The method for preparing a mouthwash for promoting postoperative wound healing according to claim 8, wherein: In S1, the heating and stirring temperature is 40°C-50°C, the rotation speed is 200r / min-400r / min, and the time is 20min-40min.

10. The method for preparing a mouthwash for promoting postoperative wound healing according to claim 8, characterized in that: In S2, the stirring speed is 100 r / min-200 r / min, and the stirring time is 10 min-15 min.

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