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Preparation and prevention and treatment application of mycoprotein in bacillus subtilis fermentation product

The technology of Bacillus subtilis and Bacillus subtilis is applied in the field of preparation and control application of bacterial protein in Bacillus subtilis fermentation products, and can solve the problems such as the difficulty of rotation and stubble, the difficulty of chemical agents, and farmland pollution, etc. Efficient induction of plant antiviral disease characteristics, reduction of pathogenic bacteria resistance, and reduction of pesticide pollution

Pending Publication Date: 2021-02-12
JIANGXI SHUNQUAN BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The use of chemical agents is relatively simple but the effect is poor, and it will also pollute the farmland. The main reason is that the tobacco mosaic virus is completely parasitic in cells, and it is difficult for chemical agents to exert their effects
And crop rotation and stubble rotation are difficult to play a role due to land restrictions

Method used

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  • Preparation and prevention and treatment application of mycoprotein in bacillus subtilis fermentation product
  • Preparation and prevention and treatment application of mycoprotein in bacillus subtilis fermentation product
  • Preparation and prevention and treatment application of mycoprotein in bacillus subtilis fermentation product

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] The invention relates to the preparation of bacterial protein in the fermentation product of Bacillus subtilis. The Bacillus subtilis strain DR-22 has a strain preservation number of CGMCC NO: 19784.

[0031] A preparation of thalline protein in a bacillus subtilis fermentation product, which mainly comprises the following steps:

[0032] (1) Activation of the bacterial strain, placing the Bacillus subtilis stored at low temperature on a plate medium for activation, and culturing at 26° C. for 24 hours to obtain an activated bacterial strain;

[0033] (2) Amplification culture, inoculating the activated bacterial strain obtained in step (1) in 100ml LB liquid medium again, at 29°C, 150r / min shaking culture for 12h, making the seed liquid of the bacterial agent;

[0034] (3) Fermentation culture, the seed liquid that step (2) makes is inoculated in the solid culture medium according to the inoculum size of 1% and carries out fermentation, at 30 ℃, 200r / min carries out co...

Embodiment 2

[0045] A kind of prevention and control application of bacterial protein in Bacillus subtilis fermentation product

[0046] 1. Test location:

[0047] The test site is located in the tobacco planting area of ​​Luonan County, Shangluo City, Shaanxi Province, and the field test is carried out.

[0048] 2. Test material:

[0049]① Invention of 500-fold dilution of Bacillus subtilis bacterial protein; (2) Commercially available chemical pesticides: (1) 24% toxin, (2) 2% Ningnanmycin; (3) Sterile water (CK control) . The tobacco is Yunyan 87, a common flue-cured tobacco variety.

[0050] 3. Test treatment:

[0051] After the tobacco seedlings have grown for 3-9 weeks, select tobacco plants with uniform growth and use various reagents for foliar spraying and root irrigation technology to cultivate tobacco. Tobacco mosaic virus infection, using foliar spray and root irrigation treatment at this stage can play a better role in preventing and treating tobacco mosaic virus disease....

Embodiment 3

[0072] Application of a Bacillus subtilis thalline protein for preventing and treating tobacco mosaic virus disease

[0073] 1. Test material:

[0074] A 500-fold dilution of the inventive Bacillus subtilis thalline protein; commercially available chemical pesticides: 40% ethephon water; sterile water (CK control). The tobacco is Yunyan 87, a common flue-cured tobacco variety.

[0075] 2. Test treatment:

[0076] In this example, tobacco cultivation is carried out by using the above three different reagents combined with root irrigation and foliar spraying techniques. Before transplanting the tobacco, soak each plant of tobacco according to 5ml per plant, and transplant and plant the tobacco after fully soaking Observation Perform plant height observation during the growth period, and compare the final data such as the weight of each plant.

[0077] Through the above test, we can find that the bacterial protein of Bacillus subtilis of the present invention has a very obviou...

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Abstract

The invention discloses preparation and prevention and treatment application of mycoprotein in a bacillus subtilis fermentation product. The preservation number of a bacillus subtilis strain DR22 is CGMCC NO: 19784. The bacillus subtilis strain DR22 has the advantages of being high in growth speed, high in sporulation quantity, high in stress resistance, rich in active products, high in mycoprotein and the like. The method is characterized in that a bacillus subtilis solid fermentation technology and a mycoprotein preparation technology are adopted, and the preparation process comprises the following steps: (1) activating strains to obtain activated strains; (2) carrying out multiplication culture to prepare a seed solution of a bacterial solution; (3) carrying out fermentation culture toprepare a microbial inoculum; and (4) preparing mycoprotein, namely fermenting thalli, drying, crushing, sieving, leaching, precipitating, carrying out isoelectric point acid precipitation, centrifugally collecting precipitates, and drying to remove moisture and mycoprotein. The final control effect can reach 73.76%, the control effect is stable, and a good biological control choice is provided for controlling tobacco mosaic virus diseases.

Description

technical field [0001] The invention belongs to the field of agricultural microorganism prevention and control, and in particular relates to the preparation and prevention and control application of bacterial protein in a Bacillus subtilis fermentation product. Background technique [0002] Tobacco is an important economic crop in my country with high economic value. Tobacco mosaic virus is one of the most widely distributed, most common and most harmful diseases in tobacco production, and it is one of the main obstacles that seriously affect the healthy growth of tobacco. After tobacco mosaic disease tobacco plants are infected with the virus, chlorophyll is destroyed, photosynthesis is weakened, leaf growth is inhibited, leaves are small and deformed, and the yield reduction rate can reach 20% to 80%. After viral disease takes place, also seriously affects the quality of tobacco leaf, makes quality deteriorate. [0003] At present, the main control methods for tobacco mo...

Claims

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Application Information

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IPC IPC(8): C12P21/00A01N63/22A01P1/00C12R1/125
CPCC12P21/00A01N63/22Y02A50/30
Inventor 陈文安德荣
Owner JIANGXI SHUNQUAN BIOTECH CO LTD
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