Perfusion bioreactor system

Abstract

The invention relates to a perfusion bioreactor system which comprises a circulating perfusion system, a constant temperature system, a gas exchange system and an on-line monitoring system, wherein the circulating perfusion system comprises a culture chamber, a liquid storage bottle and a peristaltic pump which are sequentially connected through a pipeline to form a perfusion culture loop; the constant temperature system comprises a constant temperature water bath device of which the inside is provided with a circuitous circulating pipeline, and is applicable to an electric heating and refrigerating control part; the gas exchange system is composed of a gas filter, a gas flowmeter, a high-pressure gas bottle, a pressure relief valve and a connecting pipeline; and the on-line monitoring system is composed of a monitoring probe, a monitor terminal, monitoring software, a data conversion chip and a mass transfer regulator. The perfusion bioreactor system provided by the invention can maximally simulate the in-vivo microenvironment in the tissue or organ in-vitro construction process, ensures sufficient substance exchange and normal physiological functions of seed cells in the biological material, enhance the yield of the seed cell / biological stent material, shorten the culture period and lower the culture cost.

Description

technical field [0001] The invention belongs to the field of biomedical engineering, and relates to a bioreactor for constructing an artificial organ with a tissue engineering method, in particular to a perfusion bioreactor system applied to inoculation and co-cultivation of seed cells and biological materials. Background technique [0002] Current tissue engineering research mainly includes four aspects: seed cells, biomaterials, methods and techniques for constructing tissues and organs, and clinical applications of tissue engineering. The core of tissue engineering is to establish a three-dimensional space complex composed of cells and biological materials, which is essentially different from the traditional two-dimensional structure (cell culture). With the rise of three-dimensional cell culture technology, more and more research focuses on the inoculation and co-cultivation of seed cells and biological materials, and a series of breakthroughs have been made. The concept...

AI Technical Summary

Problems solved by technology

Its disadvantages are: due to the large shear stress generated by the rotation of the stirrer, it is easy to generate a large number of air bubbles, so it is not suitable for the cultivation of cells and tissues in tissue engineering

Its disadvantages are: small operating flexibility, poor mixing performance at low flow rates, especially when the reactor is high, large in diameter, and high-density microcarrier culture

The disadvantage of rotating wall bioreactor is that it is difficult to scale up industrially, and there are always some factors that restrict each other in actual operation. Collision with the inner and outer walls and the limitation of the fragility of animal cells; higher dissolved oxygen needs to increase the corresponding oxygen partial pressure, and too high oxygen partial pressure will lead to inhibition of the removal of metabolites and generation of air bubbles, and also affect the normal operation of cells to grow

At the same time, the reactor cannot satisfy the compounding and culturing of large sections of biomaterials and seed cells.

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