161 results about "Perfusion method" patented technology

A system, ablation probe, and method is provided for treating tissue, e.g., tissue having tumors. The treatment system is configured to automatically deliver infusaid to tissue when needed and comprises an ablation probe having an ablative element and at least one perfusion exit port. The system further comprises an ablation source operably coupled to the ablative element, and a pump assembly operably coupled to the perfusion exit port(s). The pump assembly is configured for pumping infusaid out through the perfusion exit port(s), preferably during the ablation process. The system further comprises a feedback device configured for controlling the amount of infusaid displaced by the pump assembly based on a sensed tissue parameter, e.g., tissue temperature or tissue impedance.

The present invention relates to a blood purification apparatus for extracorporeally circulating the blood of a patient so as to purify the blood for medical treatment incorporating a dialyzer and a method for priming the blood purification apparatus, wherein the present invention provides a blood purification apparatus and its priming method which can simply and easily automate the priming operation and also can surely and smoothly perform the bubble purging of the dialyzer.

An object of the present invention is to provide a blood purification apparatus and a priming method thereof which can firmly perform the bubble purging smoothly and in a short time during priming operation. According to the blood purification apparatus of the present invention, the tip of the arterial blood circuit can be connected to and communicated with the tip of the venous blood circuit during priming of the blood purification apparatus before dialysis, and the blood purification apparatus can perform a priming solution charging step for supplying and charging the blood circuits with priming solution under a condition in which the tip of the arterial blood circuit and the tip of the venous blood circuit are connected to and communicated with each other; and a priming solution circulating step for forcing the charged priming solution to be flowed and circulated through the blood circuits by successively changing the driving speed of the blood pump after the priming solution charging step.

A system, ablation probe, and method is provided for treating tissue, e.g., tissue having tumors. The treatment system is configured to automatically deliver infusaid to tissue when needed and comprises an ablation probe having an ablative element and at least one perfusion exit port. The system further comprises an ablation source operably coupled to the ablative element, and a pump assembly operably coupled to the perfusion exit port(s). The pump assembly is configured for pumping infusaid out through the perfusion exit port(s), preferably during the ablation process. The system further comprises a feedback device configured for controlling the amount of infusaid displaced by the pump assembly based on a sensed tissue parameter, e.g., tissue temperature or tissue impedance.

An integrated centrifugal blood pump-oxygenator (1) which has a housing (2) with a top (3) having a blood inlet (4), a blood outlet (5) and a gas inlet (6), and a bottom (7) having a rotational body (8) being rotatably arranged in a rotor-housing (9) of the bottom (7). The integrated centrifugal blood pump-oxygenator (1) further has an oxygenator membrane (10) provided in an interior (11) of the housing (2), wherein in the operation state oxygen (12) is transferred from the gas inlet (6) through the oxygenator membrane (10) to a gas outlet (13) and blood (14) is brought in direct contact with the oxygenator membrane (10) by pumping the blood (14) with the rotational body (8) from the blood inlet (4) to the blood outlet (5). The rotational body (8) is magnetically journalled in a contact-free manner with respect to the rotor-housing (9). There is an extracorporeal life support system (1000), and a method of de-bubbling and priming a extracorporeal life support system (1000).

The present invention relates to a ring amino acid adsorbing agent and its preparation method. It uses natural macromolecule as carrier, its grain size is 0.45-0.9 mm, it is formed from resin which has amino acid ligand with fixed effective dose after activation of epoxy chloropropane, in which amino acid content is 12.5-39.7 micro mol / ml resin, and the described ligand is: amino acid, polyamino acid, polypeptide or protein. It can be directly used for blood perfusion method to cure the diseases of rheumatoid arthritis, systemic lupus erythematosus, myasthenia gravis, endotoxemia and infective shock, etc. The animal tests show that adsorbing agent has no toxic side effect and heat source. It is a good medical blood-cleaning adsorption material.

A multi-pole synchronous pulmonary artery radiofrequency ablation catheter, wherein an adjustment apparatus is arranged on a control handle; a catheter body is hollow, and a cavity is arranged therein; a lead wire, a temperature sensing wire and a pull wire are arranged in the cavity; one end of the catheter body is flexible, and the flexible end is connected to an annular ring; the annular ring is provided with an electrode group with each electrode connected to the lead wire and temperature sensing wire; the lead wire and temperature sensing wire go through the catheter body and are electrically connected to the control handle. The device uses cold saline perfusion method to protect the vascular intima and possesses advantages of simple operation, short operation time and controllable precise ablation. The device can be used to treat pulmonary hypertension with pulmonary denervation.

This invention relates to pork liver cell immortalities and its producing method. Procedures are showed: high live ratio fresh original pork liver cell is got by dispase-collagenase perfusion method; it is cultured for 24 hours. Then cell upper heat removing of recombination retronituse that contains SV40 big T antigen is used to infect the pork cell under condition of polybrene which concentration is 8ug / ml. Then it is medicine pressure filtrated by 500ug / ml G418 after one week, single clone cell is picked for large culture when the cell clone appears and grows to 1.0-2.0cm to get pork cell that can passage. Reinfection is done under condition of 8ug / ml polybrene. Then it is medicine pressure filtrated by 2ug / ml puromycin after one week. Single clone cell is picked for large culture when the cell clone appears and grows to 1.0-2.0cm to get immortality pork cell.

Apparatus use with a perfusion system, the apparatus including a subject simulator for simulating subject. The subject simulator includes a circulatory system having an inlet line for receiving fluid from the perfusion system, an outlet line for transferring fluid to the perfusion system, at least on sensor for sensing at least one fluid property and at least one control device coupled to at least one of the inlet line and the outlet line. In use this, allows the control device to manipulate the at least on fluid property based at least partially on signals from the at least one sensor, to thereby simulate a least one of compliance and resistance of the simulated subject.

The invention discloses a blood cell analyzer automatic perfusion method, including absorbing cleaning liquid, emptying and cleaning three steps; wherein the cleaning step is back flush, that is the cleaning liquid goes from the counting pool back pool into the counting pool front pool through gem hole for cleaning. A kind of blood cell analyzer cleaning liquid automatic perfusion device, including counting pool, positive and negative pressure device, liquid storage device and liquid supplier; each of the liquid supplier and liquid storage device has one end connecting to the counting pool back pool; the cleaning liquid goes from the liquid supplier into the liquid storage device under the negative pressure which is produced by positive and negative pressure device; then the cleaning liquid goes from the counting pool back pool and gem hole, and back flushes into counting pool front pool for cleaning under positive pressure which is produce by positive and negative pressure device. The blood cell analyzer cleaning liquid automatic perfusion method provided by invention has high reliability, good cleaning effect, and the blood cell analyzer cleaning liquid automatic perfusion device liquid route has simple design and low cost.

Methods and systems are disclosed for treating injured and / or ischemic tissue by delivering a platelet composition which induces neovascularization in the tissue and improves tissue perfusion.

The invention provides a method and a device for exploiting a deep-sea natural gas hydrate. At present, the development of a submarine natural gas hydrate is still at a starting stage; conventional methods of mechanical fishing, mechanical extraction, hot water perfusion, drilling platform collection and the like are used more frequently; the cost is higher; moreover, the instable natural gas hydrate is easily enabled to excessively decompose; a submarine topography is damaged; according to the method and the device for exploiting the deep-sea natural gas hydrate, on the basis of a hot water perfusion method, a movable bell-mouth-shaped gas collecting hood group and a gas collecting pipe network are arranged at the bottom of a sea; a leakage is prevented; the natural gas hydrate is gasified at a sea bed through the approaches of a temperature rise, a pressure reduction and the like; a gas bubble is collected by a gas collecting hood to be directly led to an offshore platform or an island / reef reservoir. A heat source for the temperature rise can come from the waste heat of a factory on a coast or an island; meanwhile, the submarine topography is protected by comprehensively utilizing the mill tailings of a solid mineral product for backfilling. The method and the device for exploiting the deep-sea natural gas hydrate have the advantages that energy sources are saved; the submarine topography is protected, the waste heat and a waste residue are comprehensively utilized.

The invention provides a CT scan perfusion method and device. The method includes the following steps: after a prescribed time t1 has passed since injection of a contrast agent, carrying out a single-slice CT tracking scan of the contrast agent concentration in the artery with a first radiation dosage; during scan tracking, trigging a scan program when a trigger point is reached, and carrying out an arterial phase scan of a visceral organ at a time t2 with a second radiation dosage, which is larger than the first radiation dosage, so as to obtain an arterial phase CT value; obtaining a CT value increment according to the arterial phase CT value; setting a time density curve of the contrast agent in the artery as an arterial input function during the contrast agent scan tracking process, and obtaining the blood flow volume of the visceral organ according to the arterial input function and the CT value increment. The CT scan perfusion method avoids repeated scans of the visceral organ in the traditional method, so as to reduce radiation on a human body, achieve simpler and easier operations and accurate results, and lessen the discomfort of an examinee.

The invention discloses a temperature control gas phase working medium perfusion method for a micron heat pipe, which is applied to heat dissipation of high heating flux devices. The method is characterized in that the temperature of the micron heat pipe and the temperature of a working medium container are respectively controlled; the micron heat pipe and the working medium container are respectively vacuumized; then the working medium container is connected with a working medium source, so that working medium steam can get into the working medium container; the micron heat pipe and the working medium container are communicated, so that perfusion of the gas phase working medium can be realized by utilizing the temperature difference between the micron heat pipe and the working medium container; the perfusion amount of the working medium can be controlled as per time, and after a perfusion port of the micron heat pipe is sealed, working medium perfusion for the micron heat pipe is accomplished. The working medium exists in a perfusion system in gas phase, so the perfusion rate, the repeatability and the controllability are good; the temperature of the micron heat pipe and the temperature of the working medium are respectively controlled, so that the perfusion efficiency of the working medium is improved; the method can improve the performance of the micron heat pipe.

A multi-pole synchronous pulmonary artery radiofrequency ablation catheter, wherein an adjustment apparatus is arranged on a control handle; a catheter body is hollow, and a cavity is arranged therein; a lead wire, a temperature sensing wire and a pull wire are arranged in the cavity; one end of the catheter body is flexible, and the flexible end is connected to an annular ring; the other end of the catheter body is connected to the control handle; one end of the pull wire is connected to the flexible end, and the other end of the pull wire is connected to the adjustment apparatus on the control handle, the tension of the pull wire is adjusted through the adjustment apparatus to achieve radian control of the flexible end; a shape memory wire is arranged in the annular ring, one end of the shape memory wire extends to the end of the annular ring and the other end of the shape memory wire passes through the root of the annular ring and is fixed on the flexible end of the catheter body; the annular ring is provided with an electrode group with each electrode connected to the lead wire and temperature sensing wire; the lead wire and temperature sensing wire go through the catheter body and are electrically connected to the control handle. The device uses cold saline perfusion method to protect the vascular intima and possesses advantages of simple operation, short operation time and controllable precise ablation. The device can be used to treat pulmonary hypertension with pulmonary denervation.

The invention provides a CT liver-perfusion image post-processing method and a CT liver-perfusion method. The CT liver-perfusion image post-processing method includes: obtaining a CT liver-perfusion image sequence and identifying an abdominal aorta from the CT liver-perfusion image sequence so as to obtain a time curve of a hepatic-artery contrast-agent concentration; and based on the time curve of the hepatic-artery contrast-agent concentration, calculating a contrast-agent concentration time curve of each pixel point and obtaining liver-perfusion parameters. The invention also provides a CT liver-perfusion method which uses the CT liver-perfusion image post-processing method. The CT liver-perfusion image post-processing method and the CT liver-perfusion method are higher in accuracy.

Disclosed are intra-culture perfusion methods applied to standard cell culture disposables using three-dimensional cell culture scaffold and synthetic vasculature compositions in high-throughput screening and high-content screening applications, and assay development.

The invention discloses a perfusion method of protection glue for chip insulation, comprising the following steps: 1) after the protection glue is poured in a closed container, the closed container vacuumizes the protection glue and maintains vacuum for some time; 2) a chip is arranged in a glue perfusion mold; 3) the protection glue is pressurized and poured in the glue perfusion mold; 4) and the chip is taken out after the protection glue is cured. As the closed container is adopted to vacuumize the protection glue, air bubbles in the protection glue are extruded by the protection glue to be discharged due to negative pressure, the cured protection glue forms a compact structure, a protection glue layer has good insulation effect, and therefore the surface breakdown voltage of the chip is enhanced. The invention discloses a device adopting the method to pour the protection glue at the same time.

The invention discloses a liver perfusion device, which comprises a main machine, an air-oxygen mixer assembly and a monitoring assembly. A consumable assembly is clamped on a worktable of the main machine, and when different livers need to be perfused, only the consumable assembly needs to be replaced, so that convenience is achieved; the main machine is internally provided with a temperature adjusting assembly in pipeline connection with the consumable assembly, thus the temperature of circulating water entering a first lung membrane oxygenator and a second lung membrane oxygenator for heatexchange can be adjusted through the temperature adjusting assembly, and then liver perfusion can be conducted under different temperature conditions according to the actual needs; and in addition, the main machine has different liver perfusion modes, a user can set hepatic artery and portal vein perfusion methods according to the actual needs, the perfusate oxygenation degree is adjusted according to an air-oxygen mixer, different drugs are injected by a micro-injection pump, and the effects of liver long-term preservation, improvement, repair and the like are achieved.

A system and method for generating high-resolution, small vessel perfusion maps (nCPMs) of the ocular conjunctival microvasculature. Unlike current systems and methods, the present invention allows for the generation of nCPMs using only a single raw image obtained in a single image acquisition step. The method includes obtaining a single raw image of at least a portion of the microvasculature of the ocular conjunctiva. The raw image may be obtained using a digital camera and slit lamp. The red and blue color channels are removed from the raw image to create a color-adjusted image in which contrast between the microvasculature and the sclera is enhanced. The color-adjusted image is then inverted and saved as a grayscale inverted image. The grayscale inverted image may be skeletonized. The grayscale image and / or the skeletonized image may be quantitatively analyzed.

The invention relates to a method of using wild living phyllostachys pubescens for preparing bamboo fragrant wine, and belongs to the technical field of wine brewing. The method includes the following steps: preparing the wine, choosing bamboos, pouring wine by infusion, sealing the wine, preserving bamboo to care the wine; recovering the wine, and ripening. The invention overcomes the defects of the prior art and has the following substantive characteristics and significant progresses: 1, solving the problems of complex operation and difficulty to master in the hole opening perfusion method in the production process of bamboo wine, and insufficient bamboo wine nutrients and weak bamboo scent caused by short brewing time; and 2, overcoming the defects reduced wine volume in bamboo cavity, decreased alcoholic strength, excessive sugar, wine, oversweet taste and easy necrosis of young bamboos caused by selection of young bamboos in a high-pressure needle injection method, solving the difficulties of high difficulty in operation, time and labor consumption, low production efficiency and large differences of wine capacity in each bamboo section, and completely avoiding wine pollution caused by invasion of small liquor mosquito or other small mosquito and external bacteria from the small injection hole.

The invention discloses a vacuum perfusion method for the outer wrapping edge of a wind turbine blade. The vacuum perfusion method is used for wrapping and connecting of the peripheral edges of a pressure surface and a force suction surface. The vacuum perfusion method for the outer wrapping edge comprises the steps that 1, the outer surface of a first area of the pressure surface and the outer surface of a second area, opposite to the first area, of the force suction surface are paved with reinforcing material layers in a fixed mode; 2, the outer surfaces of the reinforcing material layers are paved with release cloth and flow guiding nets in sequence; 3, the outer surfaces of the flow guiding nets are paved with sealing membranes used for sealing the outer wrapping edge of the wind turbine blade; and 4, vacuum perfusion is conducted on the interiors of the sealing membranes by using a vacuum device. According to the vacuum perfusion method for the outer wrapping edge of the wind turbine blade, the gel content of fiber cloth and bubbles in cloth layers are reduced, the fiber content is improved, mechanical properties of a product are improved, the product performance stability isstrengthened, and the product quality is improved.

Apparatus use with a perfusion system, the apparatus including a subject simulator for simulating subject. The subject simulator includes a circulatory system having an inlet line for receiving fluid from the perfusion system, an outlet line for transferring fluid to the perfusion system, at least on sensor for sensing at least one fluid property and at least one control device coupled to at least one of the inlet line and the outlet line. In use this, allows the control device to manipulate the at least on fluid property based at least partially on signals from the at least one sensor, to thereby simulate a least one of compliance and resistance of the simulated subject.

The invention belongs to the technical field of new energy materials and energy storage and conversion materials, in particular to a lithium metal anode electrode material based on TiO2 nanotube array / titanium foam and a preparation method thereof. TiO2 nanotube arrays were prepared on the surface of porous titanium foam by anodic oxidation and annealed, A lithium metal anode material with high loading of lithium metal, which can greatly alleviate the volume expansion effect of lithium metal and restrain the lithium dendrite in the process of cycling charge and discharge, was prepared by melt-perfusion method by compositing lithium metal with TiO2 nanotube array / titanium foam. A surface of that porous titanium foam three-dimensional conductive skeleton in the negative electrode material isuniformly covered with a lay of ordered TiO2 nanotube array, and lithium metal is uniformly filled with the TiO2 nanotube array / titanium foam matrix. A symmetrical cell was fabricate by using that lithium metal anode material. The cell was fabricated at 0.5 mA- cm<2> Cycling 300 cycles (600 hours) at current density, exhibiting lithium-free dendrite and stable cycling.

The invention relates to a double-loop oscillation perfusion-type biological reaction system which is applied to co-culture of seed cells and biomaterials. The biological reaction system comprises a support perfusion culture device, a liquid storage bottle, a recovery bottle and peristaltic pumps, wherein the support perfusion culture device is internally provided with a plurality of supports for co-culture of seed cells and biomaterials; the liquid storage bottle is used for conveying a culture solution in the bottle to the supports; the recovery bottle is used for receiving the culture solution flowing from the support perfusion culture device through a liquid conveying pipe; the flowing direction of the liquid storage bottle and the flowing direction of the recovery bottle are controlled through respective three-way pipes, so that the liquid storage bottle and the recovery bottle are switched between a perfusion liquid-changing channel and a perfusion culture channel; the peristaltic pump is respectively arranged in the perfusion liquid-changing channel and the perfusion culture channel and used for controlling the flow velocity of a culture solution passing through the liquid conveying pipe. According to the double-loop oscillation perfusion-type biological reaction system, the combination between an oscillation method and the perfusion method is achieved, the seed cells inside the biomaterials are subjected to sufficient substance interchange and stress stimulation, the production efficiency of engineered tissues is improved and the requirements of large-scale classed culture can be met.

A perfusion method of tree includes such steps as drilling hole on the root part of trunk until it reaches xylem or phloem, inserting the perfusion needle in the hole and perfuisng water or liquid fertilizer into tree trunk. Its advantages are high effect, and high utilization rate of water or liquid fertilizer.

The present invention relates to a tumor-targeted nanomedicine and an application and preparation method. The tumor-targeted nanomedicine comprises MSN nanoparticles with mesoporous channels and transition metal oxides and transition metal carbonyl compounds loaded in the mesoporous channels of the MSN nanoparticles. Acid-responsive nano drug (MnO2) is loaded on mesoporous silica channels by the in-situ reduction method, and then drug dodecacarbonyltriiron (Fe3CO12) before gas (CO) release is co-loaded on the mesoporous silica channels through the nano-perfusion method. The preparation processis simple, the cost is low the gas loading efficiency is high, and the nano drug has passive targeting tumor transport and response to release of ROS and CO gas in the tumor microenvironment, low-toxicity and high-efficiency of tumor chemical kinetic therapy and gas therapy are achieved, the therapy efficiency is greatly improved, and clinical application is facilitated.

The invention provides an enzyme-driven bottle-shaped nano-motor and a preparation method thereof. The method includes the steps of: taking bottle-shaped nanoparticles, glucose oxidase and catalase asthe materials, loading glucose oxidase and catalase inside the bottle-shaped nanoparticles simultaneously by vacuum perfusion method and ultrasonic perfusion method so as to prepare the enzyme-drivenbottle-shaped nano-motor. Compared with the prior art, the invention has the beneficial effects: the preparation process is simple, the prepared nano-motor has good biocompatibility and strong controllability, can move along the glucose concentration gradient in a glucose solution, realizes chemotactic movement of the enzyme-driven bottle-shaped nano-motor, and has broad application prospects indrug carrying, toxin removal, tumor therapy and other biomedical fields.

A multi-pole synchronous pulmonary artery radiofrequency ablation catheter, wherein an adjustment apparatus is arranged on a control handle; a catheter body is hollow, and a cavity is arranged therein; a lead wire, a temperature sensing wire and a pull wire are arranged in the cavity; one end of the catheter body is flexible, and the flexible end is connected to an annular ring; the other end of the catheter body is connected to the control handle; one end of the pull wire is connected to the flexible end, and the other end of the pull wire is connected to the adjustment apparatus on the control handle, the tension of the pull wire is adjusted through the adjustment apparatus to achieve radian control of the flexible end; a shape memory wire is arranged in the annular ring, one end of the shape memory wire extends to the end of the annular ring and the other end of the shape memory wire passes through the root of the annular ring and is fixed on the flexible end of the catheter body; the annular ring is provided with an electrode group with each electrode connected to the lead wire and temperature sensing wire; the lead wire and temperature sensing wire go through the catheter body and are electrically connected to the control handle. The device uses cold saline perfusion method to protect the vascular intima and possesses advantages of simple operation, short operation time and controllable precise ablation. The device can be used to treat pulmonary hypertension with pulmonary denervation.

The invention discloses a primary rat or mouse gastric mucosal epithelial cell isolation and culture method. A perfusion method is innovatively introduced into the primary rat or mouse gastric mucosal epithelial cell isolation and culture method, and sufficient perfusion without dead corners is achieved. Prepared compound enzyme digestive juice avoids the problems of low cell viability and poor yield and is capable of digesting more evenly and thoroughly as compared with preheated digestion of traditional single collagenase. Within 12 hours of cell inoculation adherence, a cell culture medium with a pH value of 6-6.5 is adopted, cell adherence rate can be increased greatly and adherence effects are better. The primary rat or mouse gastric mucosal epithelial cell isolation and culture method has the advantages of large total cell number, high isolation degree, high living rate and low contamination probability by bacteria, fungi and other cells as well as capability of achieving subculture so as to provide a favorable cell culture scheme for related fundamental researches.