22 results about "Perfusion method" patented technology

The present application relates to a combined inner core for nuclear medicine, comprising inner core assemblies, each of which is provided with at least one aperture of a hot spot hole; the inner core assemblies can be combined at will so that the combined inner core has at least six apertures of hot spot holes. A phantom structure is also provided, comprising a barrel body, the above-mentioned combined inner core and a barrel cover. An automatic bubble-free perfusion method for the phantom structure by means of an automatic perfusion device is also provided. The combined inner core of the present application is flexible to use, and can obtain the required resolution information in a single resolution evaluation. The cumbersome process of replacing the inner core and re-perfusion is avoided. The inner core assemblies of the present application are easy to process, saving production cost and time cost. The perfusion method enables the operator to minimize the risk of possible radiation hazards.

Described herein are systems, methods, and interconnecting porous hydrogel blocks for cell culture. A block may comprise a 3D continuous polymeric matrix with a network of microporous cavities, and may be configured to interconnect with at least one other block. A system may comprise at least one block and be configured to be perfused via at least one cavity by at least one perfusate under one or more controllable flow rates, pressures, volumes, viscosities, and / or osmotic pressures. A method may comprise exposing at least one block to at least one perfusate under one or more controllable flow rates, pressures, volumes, viscosities, and / or osmotic pressures.

The application discloses a kind of powder perfusion equipment and perfusion method, it is related to perfusion equipment technical field, including bunker, telescopic pipe device and pusher device, bunker is used to contain powdery material;Telescopic pipe device is equipped with inlet and outlet, inlet of telescopic pipe device is communicated with bunker one end, telescopic pipe device can change the distance between inlet and outlet;Pusher device penetrates bunker and telescopic pipe device, pusher device can push the material in bunker towards outlet.The powder perfusion equipment and perfusion method of the application can reduce the dust of material when perfusing powdery material, reduce the loss of material.

The present application belongs to the technical field of organic solid waste storage, and particularly discloses a carbon-containing liquid underground perfusion method and system, which perfuses carbon-containing liquid into the ground for solidification and storage, and the perfusion parameter adjustment mode is as follows: when the real-time distance between the upper surface of the perfusion object and the ground is greater than a height threshold, the difference between the pipe bottom pressure and the underground storage pressure is detected in real time; if the difference changes decreases, the perfusion temperature and the perfusion pressure are increased, and the perfusion viscosity is reduced; if the difference changes increases, the perfusion temperature and the perfusion pressure are reduced, and the perfusion viscosity is increased; if the difference changes does not change, the current perfusion parameters are kept. When the distance is not greater than the height threshold, the pipe bottom pressure is detected in real time; if the pressure suddenly changes, the perfusion is stopped; otherwise, the perfusion temperature is increased, and the perfusion pressure is reduced. The present application utilizes the self-polymerization characteristics of carbon-containing liquid in a high-temperature and high-pressure geological environment to gradually polymerize and solidify the carbon-containing liquid, realizes underground storage, and can realize large-scale underground carbon sequestration.

The invention discloses a method for evaluating the adsorption performance of a blood perfusion device to bilirubin. The method comprises the following steps: obtaining a hyperbilirubinemia rabbit model; and performing blood perfusion on the obtained hyperbilirubinemia rabbit model by using a blood perfusion device, calculating the adsorption rate of the blood perfusion device on bilirubin, and evaluating the adsorption performance of the adsorbent. According to the hyperbilirubinemia animal model, a rabbit is selected as a modeling object, the rabbit is subjected to intragastric administration with an induction reagent twice, and the hyperbilirubinemia rabbit model is obtained; the induction reagent is alpha-naphthyl isocyanate; the administration dosage of the induction reagent during the second time of intragastric administration is greater than that of the induction reagent during the first time of intragastric administration. According to the method, the animal model meeting the requirement for evaluating the adsorption performance of the bilirubin of the adsorbent in a perfusion mode can be rapidly established, the modeling method is simple and rapid, the modeling cost is low, the model operability is high, the adsorption performance of the adsorbent evaluated in the perfusion mode is closer to the clinical practical application condition, and the more accurate and objective evaluation effect is achieved.

The present disclosure relates to the technical field of biological sample preservation, and particularly, to an automatic perfusion apparatus and method for cryopreservation of biological tissues. The apparatus comprises a liquid supply module, a perfusion module, and a control module, wherein the liquid supply module is connected to the perfusion module through a liquid tubing, and the control module controls a liquid supply ratio and a liquid supply rate of the liquid supply module. The operation is simple. After the perfusion or recovery operation is completed on any biological sample, the operator only needs to replace the sample carrying plate or external carrying container with a new one and connect the sample carrying plate or external carrying container, after which a new round of operation can begin. Moreover, during the perfusion or recovery operation, the data processing module achieves automated control of the temperature source and the peristaltic pump according to the temperature-time working curve, the perfusion fluid flow rate-time working curve, and the perfusion fluid composition-time working curve. This ensures the continuity and consistency of the perfusion and recovery operations for each biological sample, eliminating the impact of human factors on the operation results.

This invention relates to the fields of medical device technology and signal processing algorithm technology. Specifically, it relates to an in vitro cardiac contractility dynamic enhancement system and method based on R-wave peak dual-modal sensing. The problem addressed by this invention is the technical issues of poor safety, high oxygen consumption, insufficient precision, and lack of automated closed-loop control in isolated cardiac mechanical perfusion methods. To solve these problems, this invention provides an in vitro cardiac contractility dynamic enhancement method based on R-wave peak dual-modal sensing, comprising: obtaining an initial threshold and an initial R-wave template library based on real-time cardiac physiological signals within an initial target time period; obtaining the true R-wave peak based on waveform verification of candidate R-wave peaks within the target time period and the initial threshold; calculating the stimulation delay based on species-preset parameters and real-time cardiac physiological signals; and selecting the stimulation mode and adjusting the energy parameters based on changes in the real-time cardiac physiological signals.

The application discloses a preparation method of umbilical cord decellularized matrix based on perfusion decellularization technology, which comprises decellularization treatment of a complete umbilical cord by using a programmed perfusion method, and cleaning, enzymatic hydrolysis, freeze-drying and other processes. The application further discloses the umbilical cord decellularized matrix prepared by the method and application thereof. The obtained umbilical cord decellularized matrix has complete structural integrity, retains not only umbilical cord Wharton's jelly but also rich low-immunogenic extracellular matrix components in umbilical cord tissues, and has wide application prospects in the development of soft tissue injury repair, medical beauty and life beauty related products.

The invention discloses an external perfusion system of a micro-fluidic chip and a liquid path control method, and belongs to the technical field of micro-fluidic chip perfusion. The invention aims to solve the problem of unstable flow velocity of a perfusion method of an existing micro-fluidic chip. The system comprises a liquid storage tank, a flow control tank and a micro peristaltic pump, wherein a liquid inlet of the liquid storage tank is used for injecting a culture solution; a liquid inlet of the liquid storage tank is communicated with a liquid outlet of the micro peristaltic pump, and a liquid inlet of the micro peristaltic pump is communicated with a liquid outlet of the flow control tank; a liquid inlet of the flow control tank is communicated with a liquid outlet of the micro-fluidic chip; a liquid outlet of the liquid storage tank is connected with one end of the liquid conveying pipeline, and the other end of the liquid conveying pipeline is used for being connected with a liquid inlet of the micro-fluidic chip; the liquid storage tank is higher than the flow control tank in spatial scale, and an exhaust port of the liquid storage tank is communicated with an exhaust port of the flow control tank. According to the invention, stable and uninterrupted perfusion of the micro-fluidic chip can be realized.

Provided is an apparatus for efficiently priming a microchip for particle separation. In a microchip for particle sorting, the microchip includes a sample liquid flow path, a sheath liquid flow path that merges with the sample liquid flow path at a merging unit, a merged flow path having the aforementioned merging unit at one end, a particle recovery flow path connected to the merged flow path at the other end of the merged flow path via a connection flow path, a branch flow path connected to the merged flow path at the other end of the merged flow path, and an introduction flow path for introducing a liquid into the connection flow path, and a priming method for the microchip for particle sorting includes: a first priming step of supplying a first liquid from the introduction flow path to the connection flow path, thereby priming the particle recovery flow path with the first liquid; and a second priming step of causing a second liquid to flow from the sheath liquid flow path to the merged flow path, thereby priming the branch flow path with the second liquid.

The invention discloses a circulating perfusion method which comprises the following steps: after a perfusion needle head is inserted into an artificial heart, the perfusion needle is connected to a perfusion pump through a perfusion tube, the other end of the perfusion tube is immersed below the liquid level of perfusion liquid, a double-channel circulating design is adopted, the solution is perfused from the artificial heart to a vasculature, and the solution flowing out of an auricle is collected. The coloring agent and the embedding medium are ensured to be uniformly distributed in the whole body, the permeation efficiency is improved, and the permeation uniformity index of a circulating perfusion group is remarkably superior to that of a passive diffusion method. By optimizing decalcification and degreasing, the fluorescence intensity retention rate gt of the sample is obtained; 80%. By adopting an MAA / NMA hydrogel system, polymerization is completed at 40 DEG C for 4 hours, so that the treatment period is greatly shortened, the treatment efficiency is improved, and rapid hydrogel embedding is realized. The Lange peristaltic pump is adopted to realize stable flow speed, and a temperature control system is combined, so that tissue damage is avoided. And finally, three-dimensional reconstruction of the whole tissue is realized.

The utility model relates to termite control technical field, specifically is a slow -release type termite control pesticide penetration device, including the medicine storage bucket, the medicine storage bucket one end is connected with the connecting hose through the pipeline joint, the connecting hose one end is connected with the medicine outlet pipe through the pipeline joint, the medicine outlet pipe bottom is connected with the second valve through the thread groove equidistance, the second valve bottom is connected with the first cannula through the thread groove, the medicine outlet pipe is away from the one end of connecting hose and is provided with the medicine adding assembly, inserts the second cannula one end into the ant nest, and the piston plate of connecting rod and its bottom is pushed and moves down, fills the termite control pesticide into the ant nest, and the pesticide is filled into the ant nest through high pressure perfusion method and carries out the killing to the termite, inserts multiple first cannulas into the soil above the ant nest, opens the second valve and makes the pesticide flow out through multiple first cannulas and penetrates to the soil, forms the chemical barrier, improves the killing, the prevention and control efficiency of termite.

The invention belongs to the field of organoid culture, and particularly relates to a kidney tumor organoid culture channel and a kidney tumor organoid culture method. The kidney tumor organoid culture channel comprises a PDMS porous micro-pipeline, matrix gel, RGD peptide and a plurality of organoid culture tanks; the PDMS porous micro-pipeline is wave-shaped, and organoid culture grooves are formed in wave crests and wave troughs; the matrix gel is attached to the inner wall of the PDMS porous micro-pipeline, and the surface of the matrix gel is modified with RGD peptide; a kidney tumor cell suspension is injected into the kidney tumor organoid culture channel, kidney tumor cells are settled in the organoid culture groove, a culture medium is injected, unattached cells are washed off after culture is conducted for 6-8 h, organoid culture is continuously conducted for 3-5 d through a perfusion method, and the kidney tumor organoid is obtained. And the operation process of kidney tumor organoid culture is obviously simplified.

The application discloses a kind of timing quantitative liquid automatic perfusion system and automatic perfusion method, including separable connection console and injection table, console is used for parameter setting, injection table includes control module, rotary positioning module, lifting injection module and quantitative delivery module, control module receives and stores parameters when connecting, independently controls each module coordinated operation after separation, rotary positioning module is used to carry sample test tube, and high-precision positioning is realized using photoelectric sensor and color coding stripe at the bottom of rotary disc, lifting injection module is driven by screw rod and can be lifted injection needle, quantitative delivery module realizes liquid quantitative delivery, method includes parameter configuration, equipment preparation and automatic perfusion cycle step.Such, through modular separable design, realize the low cost, small volume, simple operation and accurate operation of automatic liquid perfusion, effectively solve the problem of complex structure, high cost and large space occupation of existing equipment.

The invention discloses an infusion support, an infusion device and an infusion method thereof, and relates to the technical field of medical instruments. The infusion support comprises a supporting leg assembly, a lifting rod and a hanging frame. The bottom end of the lifting rod is connected with the supporting leg assembly, the lifting rod is used for conducting lifting motion under driving of external force, and a plurality of height gear marks are arranged on the lifting rod and correspond to different supporting heights so as to indicate the current height of the infusion support; the suspension bracket is arranged at the top end of the lifting rod and is used for suspending a myocardial protection liquid container. The structure of the infusion support is improved, the lifting rod is adopted to adjust the supporting height, the height gear marks are arranged on the lifting rod, an operator can adjust the infusion support to the target height conveniently, the precise infusion requirements of infants of different body types for myocardial protection liquid such as HTK solutions can be met, the physique of the infants can be better met, the myocardial protection effect is improved, and the infusion support is suitable for being used for infusion of the infants. And the perfusion safety is improved, and the occurrence rate of postoperative complications is reduced.

The application discloses a dynamic perfusion method and system for tunnel lining, which is characterized by the following steps: a longitudinal perfusion pipe is arranged at the center of the tunnel vault, and a slurry preparation cylinder is connected with the perfusion pipe through a perfusion pump; before the lining layer is solidified, the perfusion pump transports standard concentration slurry with a set concentration range through the perfusion pipe to perform vault cavity perfusion; when the perfusion pressure is lower than the set range, high-concentration slurry is put into the slurry preparation cylinder to be mixed with the standard concentration slurry to increase the slurry concentration to the set range; when the perfusion pressure is higher than the set range, clean water is put into the slurry preparation cylinder to be mixed with the standard concentration slurry to decrease the slurry concentration to the set range; and when the perfusion pressure reaches the set upper limit value, the perfusion is stopped. The method and system can solve the problems of slurry leakage, perfusion pipe and pipe outside blockage in the perfusion process, effectively improve the filling rate of cavity perfusion, and improve the perfusion construction effect.

The invention belongs to the technical field of medicines, and discloses a probiotic entrapped polydopamine modified porous microsphere preparation and a preparation method thereof. The preparation method comprises the following steps: firstly, preparing porous microspheres by adopting a multiple emulsion-solvent evaporation method, then constructing probiotic porous microspheres by adopting a static perfusion method or a centrifugal perfusion method, and modifying the probiotic porous microspheres by adopting polydopamine. And finally, encapsulating the probiotic porous microspheres by a layer-by-layer encapsulation method to finally prepare the probiotic-entrapped polydopamine modified porous microsphere preparation. The preparation process is simple, the cost is low, the stability is good, and the repeatability is high. The porous microsphere composite preparation disclosed by the invention is helpful for solving the problems of low bioavailability, low colonization efficiency, poor targeting property and the like of probiotics. Meanwhile, the sodium hyaluronate can promote the microsphere preparation to target an inflamed part, so that the intestinal flora of the inflamed part is improved, and the anti-inflammatory effect is improved.

An external perfusion system and liquid path control method of a microfluidic chip belong to the technical field of microfluidic chip perfusion. The present application aims at the problem of unstable flow rate of the existing microfluidic chip perfusion method. The system comprises a liquid storage tank, a flow control tank and a micro peristaltic pump. The liquid inlet of the liquid storage tank is used for injecting culture solution. The liquid inlet of the liquid storage tank is communicated with the liquid outlet of the micro peristaltic pump, and the liquid inlet of the micro peristaltic pump is communicated with the liquid outlet of the flow control tank. The liquid inlet of the flow control tank is communicated with the liquid outlet of the microfluidic chip. One end of the liquid delivery pipeline connected to the liquid outlet of the liquid storage tank, and the other end of the liquid delivery pipeline is used for connecting the liquid inlet of the microfluidic chip. The liquid storage tank is arranged higher than the flow control tank in the spatial dimension, and the exhaust port of the liquid storage tank is communicated with the exhaust port of the flow control tank. The present application can realize stable and uninterrupted perfusion of the microfluidic chip.

This invention relates to the field of medical device cleaning technology, specifically to a multi-robot collaborative intelligent cleaning workstation for flexible digestive endoscopes and its application. The workstation includes a loading robot, a wire-clamping robot, a wire-feeding robot, a flexible digestive endoscope clamping system, a vision camera, a worktable, and an enzyme infusion mechanism. All robots and mechanisms are mounted on the worktable and are centrally controlled. This invention decomposes the processes of loading, visual positioning, automatic docking and liquid injection, rotary brushing, and unloading into independent loading, wire-feeding, and wire-clamping robots, allowing these processes to be completed collaboratively by dedicated robots. The wire-clamping robot integrates liquid injection and clamping functions, avoiding frequent tool changes. Compared to existing manual perfusion methods, this invention shortens the cleaning cycle time and provides consistent cleaning results, unaffected by operator experience.

The application discloses a method for perfusion of an isolated pig kidney and application thereof. The method comprises using a peristaltic pump to perfuse physiological saline into the isolated pig kidney, and collecting generated urine. Compared with a static cold-preserved pig kidney, the isolated pig kidney obtained by using the perfusion method provided by the application can better maintain the integrity of a renal tubular structure according to a comparison of tissue sections after perfusion for 1 hour. The isolated pig kidney obtained by using the perfusion method provided by the application can effectively maintain the filtration function of the kidney during extracorporeal circulation.

The application discloses an integrated extracorporeal organ perfusion loop system and a perfusion method, and relates to the technical field of extracorporeal organ perfusion. The system comprises an organ box, a circulating pipeline, a liquid storage container, an oxygenator, a bile collection container and a controller. The circulating pipeline comprises a main outlet pipeline, a perfusion overflow outlet pipeline, a portal vein perfusion pipeline and a hepatic artery perfusion pipeline. Pressure sensors, flow sensors and blood gas monitoring components are arranged on the hepatic artery perfusion pipeline, the portal vein perfusion pipeline and the main outlet pipeline, respectively. The bile collection container is connected with a force sensor. The oxygenator is provided with a temperature control module, a temperature sensor and an oxygen concentration / flow rate sensor. The liquid storage container is connected with a liquid medicine infusion module. The controller can automatically adjust the oxygen flow of an oxygen generator, the temperature control module of the oxygenator, the flow of the perfusion pipeline and the infusion condition of the liquid medicine infusion module according to blood gas analysis results and multi-parameter monitoring data, realize linkage of blood gas monitoring and regulation, and improve the quality of organ preservation in vitro.

The invention belongs to the technical field of medicines, and discloses a probiotic-entrapped porous microsphere composite preparation and preparation and application thereof. The preparation method comprises the following steps: firstly, preparing porous microspheres by adopting a multiple emulsion-solvent evaporation method, then constructing probiotic porous microspheres by adopting a static perfusion method or a centrifugal perfusion method, and finally, encapsulating the probiotic porous microspheres by adopting a layer-by-layer encapsulation method to finally prepare the probiotic-entrapped porous microsphere composite preparation. The preparation process is simple, the cost is low, the stability is good, and the repeatability is high. The porous microsphere composite preparation disclosed by the invention is helpful for solving the problems of low bioavailability, low colonization efficiency, poor targeting property and the like of probiotics. Meanwhile, the sodium hyaluronate can promote the microsphere preparation to target an inflamed part, so that the intestinal flora of the inflamed part is improved, and the anti-inflammatory effect is improved.