Formalin safe quantitative perfusion device and safe quantitative perfusion method thereof

Abstract

The invention discloses a safe and quantitative formalin perfusion device and a safe and quantitative formalin perfusion method. Comprising a formalin material barrel cover, a formalin flow path input pipe, a second cut-off sealing valve, a peristaltic pump, a formalin flow path output pipe, a first cut-off sealing valve, a liquid output nozzle, a liquid output nozzle fixing support, a carrying tray, a case and a liquid quantitative filling electric control module. And through the suction of the peristaltic pump, the formalin always flows in the closed transparent hose made of the polytetrafluoroethylene material in the whole process, so that the volatilization and pollution risks are isolated. When the peristaltic pump stops working, a reverse instruction is given to suck the residual formalin at the liquid output nozzle back to the pipeline, the drop leakage phenomenon is eliminated, then the stop valve is manually closed, and the pipeline is completely in a sealed state. Safe and quantitative formalin perfusion is truly achieved, tissue samples are protected to the best degree, meanwhile, environmental protection is achieved, and personnel health is protected.

Description

Technical Field

[0001] The invention relates to the field of formalin perfusion, and in particular to a formalin safe quantitative perfusion device and a safe quantitative perfusion method thereof. Background Art

[0002] Formalin fixation of tissue samples is the source of quality control for the entire process of pathological examination. Timely and sufficient fixation is necessary to preserve the proteins and nucleic acids in the tissue to the maximum extent, so as to meet the requirements of morphological observation and subsequent molecular detection. How to achieve rapid and accurate perfusion, prevent the volatilization of formalin to the greatest extent, and ensure the dual safety of tissue samples and personnel during the operation is the key technical step to be solved first. At present, the products in the industry that realize accurate and automated canning of formalin can be roughly divided into two categories: the first category is imported products, which respond to the EU 2015 version of the zero pollution regulation of formalin in hospital operating rooms. The instruments and equipment that have been listed have mature technology and comprehensive functions, but are expensive. For the pathology department that currently has no charging basis, the threshold for introducing this instrument is high and it is difficult to popularize. The second category is domestic formalin perfusion equipment, which cannot achieve accurate weighing of formalin sealing, has low integration, many manual operation links, and the design and appearance of the device are simple. Centrifugal water pumps or gear pumps are mostly used as fluid delivery equipment, and the accuracy and stability of the perfusion speed are poor. Low-speed perfusion has low efficiency and the operator is exposed to formalin risks for a longer time; high-speed perfusion will cause formalin to splash at the outlet, which also poses a risk of contamination. Due to the particularity of the use of formalin liquid in the hospital pathology department, the outlet needs to be injected into a fresh tissue sample bag, and the perfusion process must be placed at the exhaust vent, so it is very necessary to estimate the weight and volume of formalin perfusion. At present, domestic devices and equipment do not respond sufficiently to the standard operating details of pathology quality control for formalin precision canning, and cannot truly achieve safe and effective control of the formalin perfusion process, let alone take into account the actual needs of automation, precision and safety. In view of the current problems, the pathology department is in urgent need of a set of formalin precision perfusion devices that can perfectly meet the above needs and are affordable. Summary of the invention

[0003] In view of the problems existing in the prior art, the present invention provides a formalin safe quantitative perfusion device and a safe quantitative perfusion method thereof, which improves the accuracy and safety of the formalin perfusion process. It truly achieves safe and quantitative formalin perfusion, protects tissue samples to the best extent, and achieves environmental protection and protects personnel health.

[0004] The technical solution of the present invention is: a formalin safe quantitative perfusion device, comprising a formalin material barrel cover, a formalin flow path inlet pipe, a second stop sealing valve, a peristaltic pump, a formalin flow path outlet pipe, a first stop sealing valve, a liquid outlet nozzle, a liquid outlet nozzle fixing bracket, a loading tray, a chassis and a liquid quantitative perfusion electrical control module;

[0005] The formalin material barrel cover is connected to the input port of the formalin flow path input pipe, and the formalin material barrel cover and the input port of the formalin flow path input pipe are welded together by a plastic hot-melt welding process at the connection position; when the formalin material barrel is replaced, only the cover needs to be replaced, which is safe and convenient.

[0006] A second shut-off sealing valve is arranged on the pipeline of the formalin flow path input pipe, the output port of the formalin flow path input pipe is connected to the input port of the peristaltic pump, the output port of the peristaltic pump is connected to the input port of the formalin flow path output pipe, and the output port of the formalin flow path output pipe is connected to the liquid output nozzle; adding the shut-off valve ensures the sealing of the flow path.

[0007] The liquid outlet nozzle is fixed on the liquid outlet nozzle fixing bracket, and a first stop sealing valve is provided on the liquid outlet nozzle;

[0008] The liquid output nozzle fixing bracket is fixed to the rear side of the chassis, the load weighing tray is fixed to the top of the chassis, and the liquid output nozzle is located above the load tray; when the tissue specimen bag is placed on the load tray, the liquid output from the liquid output nozzle can be poured into the tissue specimen bag;

[0009] The front side of the chassis is provided with operation buttons and an LED display screen, the liquid quantitative perfusion electrical control module is provided in the chassis, and the peristaltic pump is fixedly installed on the left side or the right side of the chassis and connected to the liquid quantitative perfusion electrical control module in the chassis;

[0010] The liquid quantitative perfusion electrical control module can control the peristaltic pump to absorb and transport the formalin in the formalin material barrel to the liquid output nozzle and perfuse it into the tissue specimen bag according to the required formalin liquid weight data input by the operation key, and when the liquid quantitative perfusion electrical control module detects that the weight of formalin perfused into the tissue specimen bag on the loading tray reaches the required formalin liquid weight, the peristaltic pump is controlled to stop the formalin suction work.

[0011] Furthermore, the formalin flow path inlet pipe and the formalin flow path outlet pipe are both made of polytetrafluoroethylene hoses. They are effective in resisting corrosion and aging, and will not harden or crack after long-term immersion in formalin solution, thus ensuring the airtightness and safety of the pump and pipeline. If the flow path hose needs to be replaced later, the operation is simple and quick, effectively extending the service life of the pump body.

[0012] Furthermore, the liquid outlet nozzle fixing bracket is an adjustable height bracket, which can adjust the height of the liquid outlet nozzle as needed.

[0013] Furthermore, the liquid quantitative infusion electrical control module includes a pressure sensing weighing assembly; the pressure sensing weighing assembly includes a pressure sensor and a weighing balance bracket, the weighing balance bracket is connected and fixed to the top inner wall of the chassis, and the pressure sensor is connected and fixed to the side wall of the weighing balance bracket, and the pressure sensor is used to detect and weigh the weight of the items on the loading tray.

[0014] Furthermore, the liquid quantitative perfusion electrical control module also includes a PID mainboard, a single-chip microcomputer controller, a drive motor and a 24V power transformer assembly fixed to the inner bottom of the chassis, and a main switch and a speed control knob fixed to the inner wall of the chassis;

[0015] The PID main board is connected to the operation buttons, the LED display screen, the speed control knob and the pressure sensor. The PID main board receives the required formalin liquid weight data input by the operation buttons and the drive motor starting working speed data input by the speed control knob, drives the single-chip microcomputer controller to control the drive motor to rotate at the drive motor starting working speed, and further drives the peristaltic pump connected to the drive motor to work, so as to absorb and transport the formalin in the formalin material barrel to the liquid output nozzle and pour it into the tissue specimen bag. When the PID main board detects through the pressure sensor that the weight of the formalin poured into the tissue specimen bag on the loading tray reaches the required formalin liquid weight, the peristaltic pump is controlled to stop the formalin suction work.

[0016] Furthermore, heat dissipation holes are provided at the inner bottom of the chassis.

[0017] Furthermore, the first stop sealing valve is a manual switch valve.

[0018] Further, the second stop sealing valve is a manual switch valve. When the formalin material barrel is replaced, it is manually closed to isolate the pipeline from adsorption.

[0019] The present invention also provides a formalin safe quantitative perfusion method, the specific steps are as follows:

[0020] Step 1: setting the weight of the injected formalin by operating the buttons, and setting the starting working speed data of the driving motor by the speed control knob;

[0021] Step 2: The command signal of step 1 is transmitted to the PID main board. After the signal is converted by the PID main board, it is sent to the single-chip microcomputer controller and the pressure sensor weighing component at the same time;

[0022] Step 3: The single-chip microcomputer controller sends a command program to the drive motor to drive the peristaltic pump to perform precise perfusion. The perfusion flow rate in this process is positively correlated with the precise speed of the peristaltic pump, and the speed is adjustable and controllable through the speed control knob. The speed range is 60-1200 rpm, and the liquid flow rate can be controlled in the range of 20-1500 ml / min.

[0023] Step 4: When the total weight of formalin perfusion is about to approach the initial set amount, the pressure sensor weighing component will send this data signal to the drive motor through the PID mainboard, and the peristaltic pump will reduce the speed, thereby slowing down the formalin suction speed;

[0024] Step 5: When the total weight of formalin perfusion reaches the initial set weight, the pressure sensor continues to feed back to the PID main board, driving the motor and the peristaltic pump to immediately stop formalin suction;

[0025] Step 6. After the perfusion step is confirmed to be completed, the peristaltic pump is reversed to draw the formalin in the formalin flow path inlet pipe and the formalin flow path outlet pipe back to the formalin material barrel; residual leakage from the liquid outlet nozzle is avoided. At this time, the first stop sealing valve is manually closed to ensure that there is no risk of volatilization and contamination of formalin.

[0026] The beneficial effects of the present invention are as follows: the device mainly realizes the following functions by precisely controlling the flow path speed of the peristaltic pump and modifying the process and materials:

[0027] 1. Accurately control the flow rate of the peristaltic pump to ensure that the accuracy of formalin perfusion fully meets the quality control implementation requirements of the pathology department.

[0028] 2. Through the suction action of the peristaltic pump, formalin enters the flow path input pipe from the material barrel, and then after passing through the peristaltic pump, it continues to flow through the pipeline and is injected into the tissue specimen bag through the liquid output nozzle. Formalin flows in the sealed transparent hose made of polytetrafluoroethylene throughout the process, isolating the risk of volatilization and contamination. When the peristaltic pump stops working, there will be a reverse command to suck the formalin remaining in the liquid output nozzle back into the pipeline, eliminating the dripping phenomenon, and then manually close the stop valve, and the pipeline is completely sealed. Truly safe and quantitative formalin perfusion can be carried out, protecting tissue samples to the best extent while achieving environmental protection and protecting personnel health.

[0029] 3. The peristaltic pump circuit and flow path are screened with polytetrafluoroethylene hoses with optimized pore size and thickness, which can effectively resist corrosion and aging. They will not harden or crack when immersed in formalin solution for a long time, thus ensuring the airtightness and safety of the pump circuit and pipeline.

[0030] 4. If the flow hose needs to be replaced later, the operation is simple and quick, which effectively extends the service life of the pump body.

[0031] 5. This invention will have a wide range of application scenarios in the future, such as hospital operating rooms, pathology departments, research institutes, laboratories, etc. It can be used in any scenario involving the fixation of tissue specimens, so its social and economic value is high. BRIEF DESCRIPTION OF THE DRAWINGS

[0032] Figure 1 This is a schematic diagram of the overall structure of a formalin safe quantitative perfusion device;

[0033] Figure 2 This is a schematic diagram of the pump flow path during the automatic perfusion process of formalin;

[0034] Figure 3 It is a schematic diagram of the structure of each unit component inside the chassis;

[0035] Figure 4 This is a schematic diagram of the electrical control of the formalin automatic perfusion process.

[0036] In the figure: 1 is a peristaltic pump, 2 is a formalin flow path output tube, 3 is a liquid output nozzle fixing bracket, 4 is a first stop sealing valve, 5 is a liquid output nozzle, 6 is a tissue specimen bag, 7 is a loading tray, 8 is a chassis, 9 is an operation button, 10 is an LED display screen, 11 is a formalin flow path input tube, 12 is a formalin material barrel, 13 is a second stop sealing valve, 14 is a pump body fixing plate, 15 is a support shaft, 16 is a single-chip microcomputer controller, 17 is a pressure sensing weighing component, 18 is a detection circuit interface, 19 is a main switch, 20 is a speed control knob, 21 is an input power supply, 22 is a 24V power transformer component, 23 is a heat dissipation hole, 24 is a PID mainboard, and 25 is a drive motor. DETAILED DESCRIPTION

[0037] The present invention will be further described below in conjunction with the accompanying drawings.

[0038] like Figure 1 , 2 As shown, a formalin safe quantitative perfusion device is characterized by comprising a formalin material barrel cover, a formalin flow path input pipe 11, a second stop sealing valve 13, a peristaltic pump 1, a formalin flow path output pipe 2, a first stop sealing valve 4, a liquid output nozzle 5, a liquid output nozzle fixing bracket 3, a loading tray 7, a chassis 8 and a liquid quantitative perfusion electrical control module. The formalin flow path input pipe 11 and the formalin flow path output pipe 2 are both made of polytetrafluoroethylene hoses. It can effectively resist corrosion and aging, and will not harden and crack after being immersed in formalin solution for a long time, thereby ensuring the airtightness and safety of the pump path and pipeline. If the flow path hose needs to be replaced later, the operation is simple and quick, and the service life of the pump body is effectively extended.

[0039] The formalin material barrel cover is connected to the input port of the formalin flow path input pipe 11, and the formalin material barrel cover and the input port of the formalin flow path input pipe 11 are welded together by a plastic heat-melting welding process. When the formalin material barrel is replaced, only the cover needs to be replaced, which is safe and convenient.

[0040] A second stop sealing valve 13 is provided on the pipeline of the formalin flow path input pipe 11, the output port of the formalin flow path input pipe 11 is connected to the input port of the peristaltic pump 1, the output port of the peristaltic pump 1 is connected to the input port of the formalin flow path output pipe 2, and the output port of the formalin flow path output pipe 2 is connected to the liquid output nozzle 5. Adding a stop valve ensures the flow path sealing.

[0041] The liquid outlet nozzle 5 is fixed on the liquid outlet nozzle fixing bracket 3, and a first shut-off sealing valve 4 is provided on the liquid outlet nozzle 5. Preferably, the liquid outlet nozzle fixing bracket 3 is an adjustable height bracket, including a fixing bracket, an adjustable height bracket, a liquid outlet nozzle fixing bracket, and fixing screws, and the height of the liquid outlet nozzle 5 can be adjusted as needed.

[0042] The liquid outlet nozzle fixing bracket 3 is fixed to the rear side of the chassis 8, the load weighing tray 7 is fixed to the top of the chassis 8, and the liquid outlet nozzle 5 is located above the load tray 7. When the tissue specimen bag 6 is placed on the load tray 7, the liquid output from the liquid outlet nozzle 5 can be poured into the tissue specimen bag 6.

[0043] The front side of the chassis 8 is provided with operation buttons 9 and an LED display screen 10, the liquid quantitative perfusion electrical control module is provided in the chassis 8, and the peristaltic pump 1 is fixedly installed on the left side of the chassis 8 and connected to the liquid quantitative perfusion electrical control module in the chassis 8.

[0044] The liquid quantitative perfusion electrical control module can control the peristaltic pump 1 to absorb and transport the formalin in the formalin material barrel to the liquid output nozzle and perfuse it into the tissue specimen bag according to the required formalin liquid weight data input by the operation button 9, and when the liquid quantitative perfusion electrical control module detects that the weight of formalin perfused into the tissue specimen bag 6 on the loading tray 7 reaches the required formalin liquid weight, the peristaltic pump 1 is controlled to stop the formalin suction work.

[0045] like Figure 3 , 4As shown, the liquid quantitative infusion electrical control module includes a pressure sensing weighing component 17. The pressure sensing weighing component 17 includes a pressure sensor and a weighing balance bracket, the weighing balance bracket is connected and fixed to the inner wall of the top of the chassis, and the pressure sensor is connected and fixed to the side wall of the weighing balance bracket, and the weight of the items on the loading tray 7 is detected and weighed by the pressure sensor.

[0046] The liquid quantitative perfusion electrical control module also includes a PID mainboard 24, a single-chip controller 16, a drive motor 25 and a 24V power transformer assembly 22 fixed to the inner bottom of the chassis 8, and a main switch 19 and a speed control knob 20 fixed to the inner wall of the chassis 8. The inner bottom of the chassis 8 is provided with heat dissipation holes 23.

[0047] The PID main board 24 is connected to the operation button 9, the LED display screen 10, the speed control knob 20 and the pressure sensor. The PID main board 24 receives the required formalin liquid weight data input by the operation button 9 and the drive motor starting working speed data input by the speed control knob 20, drives the single-chip microcomputer controller 16 to control the drive motor 25 to rotate at the drive motor starting working speed, and further drives the peristaltic pump 1 connected to the drive motor 25 to work, and absorbs and transports the formalin in the formalin material barrel to the liquid output nozzle and pours it into the tissue specimen bag. When the PID main board 24 detects through the pressure sensor that the weight of formalin poured into the tissue specimen bag 6 on the carrier tray 7 reaches the required formalin liquid weight, the peristaltic pump 1 is controlled to stop the formalin suction work.

[0048] Preferably, the first stop sealing valve 4 is a manual switch valve. The second stop sealing valve 13 is a manual switch valve. When the formalin material barrel is replaced, it is manually closed to isolate the pipeline from adsorption.

[0049] The specific steps of formalin safe quantitative perfusion method are as follows:

[0050] Step 1: Set the weight of the injected formalin by operating the buttons, and set the starting working speed data of the driving motor by the speed control knob.

[0051] Step 2: The command signal of step 1 is transmitted to the PID main board. After the signal is converted by the PID main board, it is sent to the single-chip microcomputer controller and the pressure sensor weighing component at the same time.

[0052] Step three, the single chip microcomputer controller sends a command program to the drive motor to drive the peristaltic pump to perform precise perfusion. The perfusion flow rate in this process is positively correlated with the precise speed of the peristaltic pump, and the speed can be adjusted and controlled by the speed control knob. The speed range is 60-1200 rpm, and the liquid flow rate can be controlled in the range of 20-1500 ml / min.

[0053] Step 4: When the total weight of formalin perfusion is about to approach the initial set amount, the pressure sensor weighing component will send this data signal to the drive motor through the PID mainboard, and the peristaltic pump will reduce the speed, thereby slowing down the formalin suction speed.

[0054] Step 5: When the total weight of formalin perfusion reaches the initial set weight, the pressure sensor continues to feed back to the PID main board, driving the motor and the peristaltic pump to immediately stop formalin suction.

[0055] Step 6: After the filling step is confirmed to be completed, the peristaltic pump will reverse and draw the formalin in the formalin flow path inlet pipe and the formalin flow path outlet pipe back to the formalin material barrel. This avoids residual leakage from the liquid outlet nozzle. At this time, the first stop sealing valve is manually closed to ensure that there is no risk of formalin volatilization and contamination.

[0056] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principle of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.

Claims

1. A formalin safe quantitative perfusion device, characterized in that: It comprises a formalin material barrel cover, a formalin flow path input pipe (11), a second stop sealing valve (13), a peristaltic pump (1), a formalin flow path output pipe (2), a first stop sealing valve (4), a liquid output nozzle (5), a liquid output nozzle fixing bracket (3), a loading tray (7), a chassis (8) and a liquid quantitative perfusion electrical control module; The formalin material barrel cover is connected to the input port of the formalin flow path input pipe (11), and the formalin material barrel cover and the input port of the formalin flow path input pipe (11) are welded together using a plastic hot melt welding process at the connection point; A second stop sealing valve (13) is provided on the formalin flow path input pipe (11); the output port of the formalin flow path input pipe (11) is connected to the input port of the peristaltic pump (1); the output port of the peristaltic pump (1) is connected to the input port of the formalin flow path output pipe (2); and the output port of the formalin flow path output pipe (2) is connected to the liquid output nozzle (5); The liquid outlet nozzle (5) is fixed on the liquid outlet nozzle fixing bracket (3), and a first stop sealing valve (4) is provided on the liquid outlet nozzle (5); The liquid output nozzle fixing bracket (3) is fixed to the rear side of the chassis (8), the load weighing tray (7) is fixed to the top of the chassis (8), and the liquid output nozzle (5) is located above the load tray (7); when the tissue specimen bag (6) is placed on the load tray (7), the liquid output from the liquid output nozzle (5) can be poured into the tissue specimen bag (6); The front side of the chassis (8) is provided with operation buttons (9) and an LED display screen (10), the liquid quantitative perfusion electrical control module is provided in the chassis (8), and the peristaltic pump (1) is fixedly mounted on the left side or the right side of the chassis (8) and connected to the liquid quantitative perfusion electrical control module in the chassis (8); The liquid quantitative perfusion electrical control module can control the peristaltic pump (1) to operate so as to absorb and transport the formalin in the formalin material barrel to the liquid output nozzle and perfuse it into the tissue specimen bag according to the required formalin liquid weight data inputted by the operation key (9); and when the liquid quantitative perfusion electrical control module detects that the weight of the formalin perfused into the tissue specimen bag (6) on the loading tray (7) reaches the required formalin liquid weight, the peristaltic pump (1) is controlled to stop the formalin suction operation.

2. A formalin safe quantitative perfusion device according to claim 1, characterized in that: The formalin flow path inlet pipe (11) and the formalin flow path outlet pipe (2) are both made of polytetrafluoroethylene hose.

3. A formalin safe quantitative infusion device according to claim 1, characterized in that: The liquid outlet nozzle fixing bracket (3) is a height-adjustable bracket, and is capable of adjusting the height of the liquid outlet nozzle (5) as required.

4. A formalin safe quantitative perfusion device according to claim 1, characterized in that: The liquid quantitative perfusion electrical control module comprises a pressure sensing weighing assembly (17); the pressure sensing weighing assembly (17) comprises a pressure sensor and a weighing balance bracket, the weighing balance bracket is connected and fixed to the inner wall of the top of the chassis, and the pressure sensor is connected and fixed to the side wall of the weighing balance bracket, and the weight of the items on the loading tray (7) is detected and weighed by the pressure sensor.

5. A formalin safe quantitative perfusion device according to claim 4, characterized in that: The liquid quantitative perfusion electrical control module further comprises a PID mainboard (24), a single-chip microcomputer controller (16), a drive motor (25) and a 24V power transformer assembly (22) fixed to the inner bottom of the chassis (8), and a main switch (19) and a speed control knob (20) fixed to the inner wall of the chassis (8); The PID main board (24) is connected to the operation button (9), the LED display screen (10), the speed control knob (20) and the pressure sensor. The PID main board (24) receives the required formalin liquid weight data input by the operation button (9) and the drive motor start working speed data input by the speed control knob (20), drives the single chip microcomputer controller (16) to control the drive motor (25) to rotate at the drive motor start working speed, further drives the peristaltic pump (1) connected to the drive motor (25) to work, absorbs and transports the formalin in the formalin material barrel to the liquid output nozzle and pours it into the tissue specimen bag. When the PID main board (24) detects through the pressure sensor that the weight of the formalin poured into the tissue specimen bag (6) on the loading tray (7) reaches the required formalin liquid weight, the peristaltic pump (1) is controlled to stop the formalin suction work.

6. A formalin safe quantitative infusion device according to claim 5, characterized in that: The inner bottom of the chassis (8) is provided with heat dissipation holes (23).

7. A formalin safe quantitative perfusion device according to claim 1, characterized in that: The first stop sealing valve (4) is a manual switch valve.

8. A formalin safe quantitative infusion device according to claim 5, characterized in that: The second stop sealing valve (13) is a manual switch valve.

9. A method for safe quantitative perfusion of formalin implemented by a safe quantitative perfusion device of formalin according to any one of claims 1 to 8, characterized in that: The specific steps are as follows: Step 1: setting the weight of the injected formalin by operating the buttons, and setting the starting working speed data of the driving motor by the speed control knob; Step 2: The command signal of step 1 is transmitted to the PID main board. After the signal is converted by the PID main board, it is sent to the single-chip microcomputer controller and the pressure sensor weighing component at the same time; Step 3: The single-chip microcomputer controller sends a command program to the drive motor to drive the peristaltic pump to perform precise perfusion. The perfusion flow rate in this process is positively correlated with the precise speed of the peristaltic pump, and the speed is adjustable and controllable through the speed control knob. The speed range is 60-1200 rpm, and the liquid flow rate can be controlled in the range of 20-1500 ml / min. Step 4: When the total weight of formalin perfusion is about to approach the initial set amount, the pressure sensor weighing component will send this data signal to the drive motor through the PID mainboard, and the peristaltic pump will reduce the speed, thereby slowing down the formalin suction speed; Step 5: When the total weight of formalin perfusion reaches the initial set weight, the pressure sensor continues to feed back to the PID main board, driving the motor and the peristaltic pump to immediately stop formalin suction; Step 6: After the perfusion step is confirmed to be completed, the peristaltic pump is reversed to draw the formalin in the formalin flow path inlet pipe and the formalin flow path outlet pipe back to the formalin material barrel; then the first stop sealing valve is manually closed.

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