Filtering device for cell fluid after amniotic tissue digestion

Abstract

The utility model relates to a kind of filtering devices for amniotic membrane tissue digestion cell liquid, belong to the technical field of cell liquid processing equipment.It is formed by the cooperation of first filter assembly, second filter assembly and filter cup assembly, and forms sealed flow channel in vertical direction, accelerates high viscous liquid flow in the whole filtration process, reduces blockage;At the same time, the flow guide pipe is connected to the lowest point of filter cup, and cooperates with liquid stop clamp to realize rapid collection, reduce sample loss;Single operation completes filtration operation, eliminates external pollution and eliminates the risk of liquid leakage.

Description

Technical Field

[0001] This utility model relates to the field of cell fluid treatment equipment technology, and in particular to a filtration device for cell fluid after digestion of amniotic tissue. Background Technology

[0002] With the development of biomedical engineering technology, cell separation technology based on tissue digestion has emerged. This technology converts amniotic tissue into a single-cell suspension through enzymatic hydrolysis, which has advantages such as high cell viability retention rate and short operation cycle. This has led to the development of cell purification processes based on multi-stage filtration.

[0003] In related technologies, a progressive filtration scheme is generally adopted to remove incompletely digested tissue fragments: first, a large-pore filter is used for pre-filtration to separate large-sized residues, and then a small-pore filter is used for fine filtration to remove tiny impurities. The entire process requires repeated transfer of liquid between multiple independent filters.

[0004] However, the above multi-level filtering method has the following drawbacks:

[0005] First, based on the filtration of cell fluid after amnion tissue digestion, the filter needs to be replaced frequently to maintain filtration efficiency.

[0006] Second, multiple transfer operations significantly increase the risk of microbial contamination, posing a safety hazard, especially in the field of cell therapy where aseptic requirements are stringent.

[0007] Third, there is a risk of leakage during multi-container transfer, which can lead to irreversible loss of cell samples. Utility Model Content

[0008] In response to the shortcomings of the existing production technology, the applicant provides a filtration device for the cell fluid after digestion of amniotic tissue, thereby eliminating the need for multiple container replacements during filtration and reducing the risk of contamination and leakage.

[0009] The technical solution adopted in this utility model is as follows:

[0010] A filtration device for cell fluid after digestion of amniotic tissue, comprising:

[0011] The first filter assembly, the second filter assembly, and the filter cup assembly are connected sequentially from top to bottom;

[0012] The second filter assembly includes an integrally formed second cavity, an inclined portion, and a first docking portion. The inclined portion connects the second cavity and the first docking portion, and the inner diameter of the inclined portion gradually decreases from top to bottom to increase the cell fluid flow rate.

[0013] The bottom of the filter cup assembly is inclined, and its inclined end is connected to the guide tube. The outlet end of the guide tube is provided with a tube cap, and the tube body is provided with a liquid stop clamp.

[0014] The bottom of the filter cup assembly is supported by the base assembly;

[0015] The base assembly includes a support base and an inclined platform. The inclined surface of the platform is in contact with the bottom of the filter cup assembly, and the inclination angle of the inclined surface matches the inclination angle of the bottom of the filter cup assembly.

[0016] As a further improvement to the above technical solution:

[0017] In one embodiment, the first filter assembly includes a first cavity, inside which a first filter screen and a second filter screen are arranged sequentially from top to bottom, and a first external thread area is provided at the bottom of the first cavity.

[0018] In one embodiment, a third filter and a fourth filter are arranged sequentially from top to bottom within the second cavity.

[0019] In one embodiment, the top of the second cavity is provided with a first internal thread area, which is screwed into the first external thread area of ​​the first filter assembly; the bottom of the first mating part is provided with a second external thread area.

[0020] In one embodiment, the filter cup assembly includes a filter cup cavity and a second mating portion at the top, wherein the inner surface of the second mating portion is provided with a second internal thread area, which is screwed into the second external thread area of ​​the second filter assembly.

[0021] In one embodiment, the flow guide is connected to the lowest position of the inclined end of the filter cup assembly.

[0022] In one embodiment, the first filter assembly, the second filter assembly, and the filter cup assembly form a sealed channel through threaded connection.

[0023] In one embodiment, the first filter screen has a 50-mesh filter screen structure, and the second filter screen has a 100-mesh filter screen structure.

[0024] In one embodiment, the third filter is a 200-mesh filter and the fourth filter is a 300-mesh filter.

[0025] In one embodiment, the bottom of the filter cup assembly is tilted at an angle of 5°-15°.

[0026] The beneficial effects of this utility model are as follows:

[0027] This utility model has a compact structure. The first filter assembly, the second filter assembly, and the filter cup assembly are connected to form a vertical sealed channel, which avoids the transfer of liquid between independent containers and eliminates the risk of contamination caused by transfer operations. At the same time, the inclined bottom of the filter cup assembly forms a slope, which uses gravity to accelerate the flow of highly viscous cell fluid, making it easier for the cell fluid to flow into the guide tube.

[0028] This utility model also has the following advantages:

[0029] (1) The first and second filter components of this utility model work together to form a multi-stage progressive filtration, completing the process from coarse filtration to fine filtration in a single operation, which significantly improves efficiency.

[0030] (2) The inclined part of the second filter component of this utility model can accelerate the flow rate of cell fluid and prevent gel accumulation on the filter surface.

[0031] (3) The guide tube of this utility model is connected to the lowest point of the inclined end of the filter cup assembly, and the flow rate is precisely controlled with the liquid stop clamp to avoid liquid stagnation and reduce cell loss. Attached Figure Description

[0032] Figure 1 This is a schematic diagram of the overall structure of this utility model.

[0033] Figure 2 for Figure 1 A schematic diagram of the structure under explosive conditions.

[0034] Figure 3 This is a cross-sectional view of the internal structure of this utility model.

[0035] Figure 4 This is a schematic diagram of the external structure of the first filter component of this utility model.

[0036] Figure 5 This is a schematic diagram of the external structure of the second filter component of this utility model.

[0037] Wherein: 100, first filter assembly; 200, second filter assembly; 300, filter cup assembly; 400, guide tube; 500, tube cap; 600, stop clamp; 700, base assembly;

[0038] 110. First cavity; 120. First filter screen; 130. Second filter screen; 140. First external thread area;

[0039] 210. Second cavity; 220. Inclined portion; 230. First mating portion; 240. Third filter screen; 250. Fourth filter screen; 260. First internal thread area; 270. Second external thread area;

[0040] 310. Filter cup cavity; 320. Second mating part; 330. Second internal thread area;

[0041] 710. Stage; 720. Support base. Detailed Implementation

[0042] The specific embodiments of this utility model are described below with reference to the accompanying drawings.

[0043] To facilitate understanding of this utility model, a more complete description will be given below with reference to the accompanying drawings. Preferred embodiments of this utility model are shown in the drawings. However, this utility model can be implemented in many different forms and is not limited to the embodiments described herein. Rather, these embodiments are provided to provide a more thorough and complete understanding of the disclosure of this utility model.

[0044] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention pertains. The terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. The term "and / or" as used herein includes any and all combinations of one or more of the associated listed items.

[0045] When using the terms “including,” “having,” and “comprising” as described herein, another component may be added unless explicitly qualifying terms such as “only,” “consisting of,” etc. are used. Unless otherwise stated, singular terms may include plural forms and should not be construed as having a quantity of one.

[0046] It should be understood that although the terms "first," "second," etc., may be used herein to describe various elements, these elements should not be limited by these terms. These terms are used only to distinguish one element from another. For example, without departing from the scope of this invention, a first element may be referred to as a second element, and similarly, a second element may be referred to as a first element.

[0047] Furthermore, the accompanying drawings are not drawn to a 1:1 scale, and the relative dimensions of the components are shown in the drawings only as examples and not necessarily to actual scale.

[0048] like Figures 1-5 The accompanying drawing shows a schematic diagram of the structure of a filtration device for cell fluid after digestion of amniotic tissue according to an embodiment of the present invention; for ease of description, the drawing only shows the structure related to the embodiment of the present invention.

[0049] This application provides a filtration device for cell fluid after digestion of amniotic tissue, comprising a first filtration component 100, a second filtration component 200, and a filter cup component 300 connected sequentially from top to bottom to form a sealed flow channel.

[0050] In this embodiment, the first filtration assembly 100 includes a first cavity 110, the top of which is open for injecting amniotic fluid. Inside the first cavity 110, a 50-mesh first filter 120 and a 100-mesh second filter 130 are detachably connected from top to bottom to grade and intercept large-sized tissue residues. For example, the detachable connection between the first filter 120 and the second filter 130 is one of a pin connection, a key connection, and a snap-fit ​​connection.

[0051] Furthermore, the bottom of the first cavity 110 is provided with a first external thread area 140 for screwing with the second filter assembly 200.

[0052] In this embodiment, the second filter assembly 200 includes a second cavity 210, inside which a 200-mesh third filter 240 and a 300-mesh fourth filter 250 are detachably connected from top to bottom. Fine filtration is achieved through the cooperation of the third filter 240 and the fourth filter 250. For example, the detachable connection between the third filter 240 and the fourth filter 250 is one of a pin connection, a key connection, and a snap-fit ​​connection.

[0053] The purpose of designing the first filter 120, the second filter 130, the third filter 240 and the fourth filter 250 as detachable is to facilitate the later maintenance and cleaning of the filters, and to reuse them after sterilization in order to achieve aseptic operation, while also avoiding the high cost of disposable consumables.

[0054] Furthermore, an inclined portion 220 is provided below the second cavity 210, and the second cavity 210 is integrally connected to the first docking portion 230 through the inclined portion 220; wherein, the inner diameter of the inclined portion 220 gradually narrows from top to bottom, forcing the cell fluid flow rate to increase and preventing gel accumulation on the filter surface.

[0055] Furthermore, the top of the second cavity 210 is provided with a first internal thread area 260, which is screwed to the first external thread area 140 of the first filter assembly 100; the bottom of the first docking part 230 is provided with a second external thread area 270.

[0056] In this embodiment, the filter cup assembly 300 includes a filter cup cavity 310 for receiving cell fluid that has undergone multi-stage filtration; furthermore, the bottom of the filter cup cavity 310 is inclined, and the inclination angle is 5°-15°, preferably 10°, and the lowest point of the inclined end is connected to the guide tube 400 to ensure that there is no liquid residue.

[0057] Furthermore, a second mating portion 320 is provided at the top of the filter cup cavity 310, and a second internal thread area 330 is provided on the inner surface of the second mating portion 320, which is screwed to the second external thread area 270 of the second filter assembly 200.

[0058] In this embodiment, a guide tube 400 is also included, which is used to connect to the lowest point of the inclined bottom of the filter cup assembly 300, and a tube cap 500 is provided at the end of the guide tube 400 for sealing.

[0059] Furthermore, a liquid-stopping clamp 600 can be optionally clamped on the body of the flow guide tube 400 to control the liquid flow rate and reduce sample loss.

[0060] In this embodiment, the base assembly 700 includes a support base 720, which is used to fix the stage 710 and provide overall support; further, the upper surface of the stage 710 is inclined and fits against the bottom of the filter cup assembly 300.

[0061] Furthermore, the tilt angle of the stage 710 matches the tilt angle of the bottom tilt end of the filter cup assembly 300 to ensure stable fit of the filter cup.

[0062] In practice, the workflow of this utility model is as follows:

[0063] Amniotic fluid digestion occurs sequentially as follows:

[0064] The first filter element 100: 50 mesh → 100 mesh, removes large residues through a series of filter screens;

[0065] The second filter component 200: 200 mesh filters are used in conjunction with a 300 mesh filter for fine filtration, and the inclined part 220 accelerates the liquid flow.

[0066] The filter cup assembly 300 allows cell sap to flow into the guide tube 400 along its sloping bottom, and collection is controlled by a stop clamp 600.

[0067] In summary, this invention, through the cooperation of the first filter assembly 100, the second filter component 200, and the filter cup assembly 300, forms a sealed channel in the vertical direction and has a multi-stage gradient filtration structure. In addition, the inclined portion 220 of the second filter component 200 accelerates cell fluid, while the gradient layout of the filter screen structure (50→300 mesh) can avoid clogging, and the filtration operation is completed in a single operation. Furthermore, the different components of this invention are connected by threads, and the modular configuration facilitates later maintenance.

[0068] The technical features of the above embodiments can be combined in any way. For the sake of brevity, not all possible combinations of the technical features in the above embodiments are described. However, as long as there is no contradiction in the combination of these technical features, they should be considered to be within the scope of this specification.

[0069] The embodiments described above merely illustrate the implementation of this utility model, and while the descriptions are relatively specific and detailed, they should not be construed as limiting the scope of the utility model patent. It should be noted that those skilled in the art can make various modifications and improvements without departing from the concept of this utility model, and these all fall within the protection scope of this utility model. Therefore, the protection scope of this utility model patent should be determined by the appended claims.

Claims

1. A filtration device for cell fluid after digestion of amniotic tissue, characterized in that, include: The first filter assembly (100), the second filter assembly (200), and the filter cup assembly (300) are connected sequentially from top to bottom; The second filter assembly (200) includes an integrally formed second cavity (210), an inclined portion (220) and a first docking portion (230). The inclined portion (220) connects the second cavity (210) and the first docking portion (230), and the inner diameter of the inclined portion (220) gradually decreases from top to bottom to increase the cell fluid flow rate. The bottom of the filter cup assembly (300) is inclined, and its inclined end is connected to the guide tube (400). The outlet end of the guide tube (400) is provided with a tube cap (500), and the tube body is provided with a liquid stop clamp (600). The bottom of the filter cup assembly (300) is supported by the base assembly (700); The base assembly (700) includes a support base (720) and an inclined platform (710), the inclined surface of which is in contact with the bottom of the filter cup assembly (300), and the inclination angle of the inclined surface matches the inclination angle of the bottom of the filter cup assembly (300).

2. The filtration device for cell fluid after digestion of amniotic tissue according to claim 1, characterized in that, The first filter assembly (100) includes a first cavity (110), and a first filter screen (120) and a second filter screen (130) are arranged sequentially from top to bottom inside the first cavity (110). A first external thread area (140) is provided at the bottom of the first cavity (110).

3. The filtration device for cell fluid after digestion of amniotic tissue according to claim 2, characterized in that, The second cavity (210) is provided with a third filter (240) and a fourth filter (250) arranged from top to bottom.

4. The filtration device for cell fluid after digestion of amniotic tissue according to claim 3, characterized in that, The top of the second cavity (210) is provided with a first internal thread area (260), which is screwed to the first external thread area (140) of the first filter assembly (100); The bottom of the first mating part (230) is provided with a second external thread area (270).

5. The filtration device for cell fluid after digestion of amniotic tissue according to claim 4, characterized in that, The filter cup assembly (300) includes a filter cup cavity (310) and a second docking portion (320) at the top. The inner surface of the second docking portion (320) is provided with a second internal thread area (330), which is screwed to the second external thread area (270) of the second filter assembly (200).

6. The filtration device for cell fluid after digestion of amniotic tissue according to claim 1, characterized in that, The guide tube (400) is connected to the lowest position of the inclined end of the filter cup assembly (300).

7. The filtration device for cell fluid after digestion of amniotic tissue according to any one of claims 1-6, characterized in that, The first filter assembly (100), the second filter assembly (200), and the filter cup assembly (300) form a sealed channel through threaded connection.

8. The filtration device for cell fluid after digestion of amniotic tissue according to claim 2, characterized in that, The first filter (120) has a 50-mesh filter structure, and the second filter (130) has a 100-mesh filter structure.

9. The filtration device for cell fluid after digestion of amniotic tissue according to claim 3, characterized in that, The third filter (240) has a 200-mesh filter structure, and the fourth filter (250) has a 300-mesh filter structure.

10. The filtration device for cell fluid after digestion of amniotic tissue according to claim 1, characterized in that, The bottom tilt angle of the filter cup assembly (300) is 5°-15°.

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