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Method for producing active factors by using perinatal mesenchymal stem cells (MSCs) and beautifying preparation

An active factor, perinatal technology, applied in the direction of cell culture active agent, skin care preparations, biochemical equipment and methods, etc., can solve the problem of huge amount of cell metabolic waste, hinder the application of active factor, etc., to improve production , Improve sensitive skin, improve the effect of the effect

Pending Publication Date: 2020-08-25
广东壹加再生医学研究院有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Moreover, in production, it is necessary to use high-density large-scale cell culture expansion, and the amount of cell metabolic waste generated is also huge.
This greatly hinders the future application of active factors produced by perinatal MSCs in cosmetics

Method used

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  • Method for producing active factors by using perinatal mesenchymal stem cells (MSCs) and beautifying preparation
  • Method for producing active factors by using perinatal mesenchymal stem cells (MSCs) and beautifying preparation
  • Method for producing active factors by using perinatal mesenchymal stem cells (MSCs) and beautifying preparation

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Embodiment approach

[0103] Embodiment: According to the description of the present invention, human umbilical cord mesenchymal stem cells were isolated from healthy human perinatal tissues, and a total of 5 cell lines were constructed. 5th generation. Remove the complete medium at the same time according to the degree of cell confluency and start collecting the cell secretion.

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Abstract

The invention provides a method for producing active factors by using perinatal mesenchymal stem cells (MSCs) and a beautifying preparation. The method comprises the following steps: carrying out hypoxic starvation culture on P1-generation MSCs; carrying out hypoxic starvation culture on P2-generation MSCs, and collecting a culture supernatant, and then using a complete culture medium to passage to a P3 generation; carrying out hypoxic starvation culture on P3-generation MSCs, and collecting a culture supernatant, and then using a complete culture medium to passage to a P4 generation; and carrying out hypoxic starvation culture on P4-generation MSCs, and collecting a culture supernatant, and then using a complete culture medium to passage to a P5 generation. The culture method can significantly promote MSCs to secret more active factors, and can particularly increase the yield of the active factors after low-temperature induction treatment. Secondly, a recovered cell factor stock solution can improve the effects of the beautifying preparation after filtration by activated carbon.

Description

technical field [0001] The invention relates to a method for producing active factors by using perinatal MSCs and a cosmetic preparation, belonging to the field of biotechnology. Background technique [0002] Mesenchymal stem cells (MSC, mesenchymal stem cells) are an important member of the stem cell family, derived from the mesoderm in the early development stage, and belong to pluripotent stem cells. MSC was originally found in the bone marrow because of its multi-directional differentiation potential, hematopoietic support and promotion Stem cell implantation, immune regulation and self-replication have attracted increasing attention. And mesenchymal stem cells have the characteristics of adherent growth in vitro. People have used this characteristic to separate them from various tissues and blood sources such as bone marrow, fat, peripheral blood, placenta, umbilical cord and umbilical cord blood, and to grow them in vitro. Scale-up preparation. [0003] Under specifi...

Claims

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Application Information

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IPC IPC(8): A61K8/99A61K8/64A61K8/73A61Q19/00A61Q19/08C12N5/0775
CPCA61K8/99A61K8/64A61K8/735A61Q19/00A61Q19/08C12N5/0668C12N2500/02C12N2501/24
Inventor 王东福陈智聪卢丽红黄里许峻荣
Owner 广东壹加再生医学研究院有限公司
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