46 results about "Stem Cell Collection" patented technology

The present invention provides improved compositions and methods for the collection of stem cells from an organ, e.g., placenta. The invention provides a stem cell collection composition comprising an apoptosis inhibitor and, optionally, an enzyme such as a protease or mucolytic enzyme, vasodilator, necrosis inhibitor, oxygen-carrying perfluorocarbon, or an organ preserving compound. The invention provides methods of using the stem cell collection composition to collect stem cells and to preserve populations of stem cells.

The invention discloses a method for preparing essence of stem cell factor with an anti-aging effect. The method comprises the steps that step 1, human umbilical cord mesenchyma stem cells are prepared and cultured, stem cell culture supernatant is collected, concentrate is obtained after concentration is conducted, and the concentrate is further frozen and dried to prepare the lyophilized powderof the stem cell factor; step 2, the components of, by volume, 0.1-1% of hyaluronic acid, 1-10% of glycerol, 0.1-1% of collagen protein, 0.1-1% of tea polyphenols and the balance water are mixed to obtain solvent; step 3, the solvent is added into the lyophilized powder of the stem cell factor to be thoroughly dissolved to obtain the essence of the stem cell factor. The prepared essence of the stem cell factor comprises multiple kinds of cell active factor components such as epidermal growth factor, vascular endothelial growth factor, hepatocyte growth factor, nerve growth factor and the like,the essence can nourish skin, promote skin repairing and regenerating, resist wrinkles, remove colored patches and the like, and the essence has better application value in the field of medical beauty.

The invention provides a separation and purification method of a human umbilical cord mesenchymal stem cell exosome and an application of the human umbilical cord mesenchymal stem cell exosome, and belongs to the technical field of medicines. The human umbilical cord mesenchymal stem cell exosome provided by the invention is prepared by cultivating human umbilical cord mesenchymal stem cells via aserum-free medium, collecting supernatant liquid, implementing centrifuging as well as ultra-filtration and concentration, transferring a concentrated solution onto a 30% sucrose-heavy water densitypad and implementing further purification through sucrose density centrifuging, so that the human umbilical cord mesenchymal stem cell exosome is obtained. According to the separation and purificationmethod that the human umbilical cord mesenchymal stem cell exosome is obtained through separation and purification, immunoreactivity is effectively reduced, and a controllable dosage when the human umbilical cord mesenchymal stem cell exosome is used is guaranteed. The human umbilical cord mesenchymal stem cell exosome, by improving a degree of activating an insulin signaling pathway of a type IIdiabetes animal model, can inhibit composition and decomposition of hepatic glycogen, so that glucose metabolism homeostasis can be achieved; and meanwhile, the sensitivity of the type II diabetes animal model to insulin and an insulin secretion function of pancreatic [beta] cells can be improved and a blood glucose concentration can be reduced, so that the application of the human umbilical cordmesenchymal stem cell exosome to the preparation of medicines for treating type II diabetes can be achieved.

The subject matter of this invention is a novel method to augment the process of obtaining populations of placental hematopoietic stem and progenitor cells for use in medical practices. A method of augmentation of stem cells collection from placenta is claimed comprising the steps of (a) infusing placental vessels cell preservation compound and a with a composition containing blockers of cell adhesion receptors, (b) incubating said placenta for a sufficient period of time, (c) placing placenta in a containment with sufficient intensity of electromagnetic or ultrasound field for a sufficient period of time; (d) eluting cell suspension from placental vessels, (e) collecting cell suspension and harvesting cells. Invention further claims a method of treatment a disease by means of administering therapeutic composition containing said placental-derived hematopoietic stem cells.

Apparatus and methods for collecting stem cells from an umbilical cord utilize a plurality of needles that are inserted into the umbilical cord. The needles may be fenestrated, and may have a common output to a line via which the stem cells are collected. A negative pressure source, such as a vacuum, may be used to draw the cells through the needles and the line. Apparatus for holding the umbilical cord, compressing the umbilical cord, positioning the plurality of needles in relation to the umbilical cord, guiding insertion of the needles toward and into the umbilical cord, withdrawing blood from the umbilical cord, and / or collecting blood withdrawn from the umbilical cord may be provided. One or more one-way check valves may be used to control fluid flow. Additionally or alternatively, a shunt may be used during blood collection to prevent backflow through the needles.

The invention relates to the field of biotechnology and particularly relates to a menstrual blood preserving fluid and an application thereof and a separation culture method of menstrual blood-source endometrium stem cells, wherein the menstrual blood is the source of the menstrual blood-source endometrium stem cells. The menstrual blood of women is used as the source for stem cell collection, so that the waste is utilized and turned into wealth while the source is abundant; the number of endometrium stem cells in the menstrual blood is 30 times that in bone marrow, and the active ingredients are plentiful; and the menstrual blood is preserved by the menstrual blood preserving fluid so as to prevent the menstrual blood from being contaminated by the outside in a process from collection to separation. In the invention, the menstrual blood is preserved by the menstrual blood preserving fluid with a unique formula, and the contamination rate of the menstrual blood is remarkably reduced while the purity and activity of the obtained menstrual blood-source endometrium stem cells are guaranteed.

The invention discloses a preparation method and application of human mesenchymal stem cell supernatant dry powder gel. According to the invention, serum-free mesenchymal stem cell complete culture solution is used for culture and passage of human umbilical cord mesenchymal stem cells, and 3-5 generations of mesenchymal stem cell supernatant with low passage is collected to prepare freeze-dried powder. According to the preparation method of the freeze-dried powder of the human umbilical cord mesenchymal stem cell culture supernatant, the prepared freeze-dried powder effectively preserves a mixture of various cell factors with biological activity in the human umbilical cord mesenchymal stem cell supernatant, and can effectively solve the problems that the storage life of the supernatant is limited; and a foundation is laid for clinical and industrial application of the human mesenchymal stem cell culture solution.

The invention discloses a method for extracting hematopoietic stem cells in placenta by utilizing a multifunctional placenta tissue processing device, relates to the technical field of biology, in particular to a processing method of placenta tissues. The invention aims to solve the problems that during conventional placenta cell cleaning and separating, pollution can be easily caused due to placenta tissue open processing, artificial time is consumed during a processing procedure, processing is incomplete, and less tissue space cells are obtained. A placenta tissue processing method comprisesthe following steps: 1, cleaning the placenta; 2, crushing the placenta; 3, collecting the hematopoietic stem cells of the placenta, i.e., completing tissue processing of the placenta before separating stem cells. The method disclosed by the invention is mainly used for carrying out tissue processing on the placenta.

The invention provides a method and system for separating, culturing and amplifying human umbilical cord mesenchymal stem cells. The method comprises the following steps: step I, pretreating a sample; step II, obtaining Wharton's jelly, cutting the obtained Wharton's jelly into small tissue, and adding a filtered red blood cell lysis buffer solution of which the volume is 3.5 times that of the small tissues; step III, digesting collagenase, wherein a digestion solution is a balanced salt solution containing 1% of IV type collagenase, 0.5% of hyaluronidase, 300U / ml DNA enzyme and 2% of a serum substitute; step IV, performing primary cell isolated culture: adding a serum-free culture medium of mesenchymal stem cells to obtain a cell suspension, and adding a special primary cell culture solution for umbilical cord mesenchymal stem cells for primary culture; step V, subculturing the cells, and inoculating the collected cells according to 5000 cells / cm < 2 > by using trypsin with the mass percent concentration of 0.215%; and step VI, collecting the umbilical cord mesenchymal stem cells, and performing osteogenic differentiation, adipogenic differentiation and chondrogenic differentiation experiments.

The invention relates to a device and a method for separating hematopoietic stem cells from cord blood. The device for separating hematopoietic stem cells from cord blood comprises a blood sampling bag, a plasma bag, an erythrocyte collecting bag, an eluant bag, a stem cell collecting bag, a leukocyte filter and a pressurizing pump, wherein a coagulum filtering net is arranged between the blood sampling bag and the plasma bag; another coagulum filtering net and the leukocyte filter are arranged between the blood sampling bag and the eluant bag; the pressurizing pump is arranged between the plasma bag and the erythrocyte collecting bag and is used for achieving reverse filling of plasma. The device is simple, the use method is simple and convenient, the recycling rate of the prepared hematopoietic stem cells is greater than 83%, the hematopoietic stem cells are high in activity, less time is spent, the recycling efficiency is high, a new method is provided for hematopoietic stem cell preparation, and relatively good practical application values and popularization values are achieved.

The invention discloses an upper limb supporting frame assembly for stem cell collection, and relates to the technical field of medical stents. The upper limb supporting frame assembly comprises an upper limb supporting part, wherein the upper limb supporting part comprises a length-adjustable forearm arc-shaped plate; one end of the forearm arc-shaped plate is rotationally connected with a hand arc-shaped plate; the bottom of the other end of the forearm arc-shaped plate is rotationally connected with a universal joint; and the movable end of the universal joint is fixedly connected with a shoulder arm arc-shaped plate. The upper limb supporting frame assembly has a reasonable structure, the angle and the position of each joint of the upper limb supporting frame part can be adjusted to meet the optimal comfort of a patient, the arm arc-shaped plate is bent at an angle relative to an armpit supporting block, arm and shoulder arm bending of the patient is simulated, and the forearm arc-shaped plate rotates and swings relative to the armpit supporting block to simulate a situation that the arm of the patient rotates and swings relative to the shoulder arm. Therefore, the normal movement operation of the upper limbs of the patient is simulated, pain and numbness caused by long-time immobility of the upper limbs of the patient are relieved, and comfort of the human body is achieved.

The invention relates to the technical field of medical equipment, and relates to a device for preserving umbilical cord blood stem cells. The device comprises a box body, pressure separating plates,a lifting driving mechanism and a fixing mechanism. The two or more pressure separating plates are arranged, and all the pressure separating plates are arranged in the box body in sequence in the height direction and can ascend and descend in the box body. A connecting mechanism is arranged between every two adjacent pressure separating plates. In the height direction, each pressure separating plate located on the upper portion can relatively get close to or keep away from the corresponding adjacent pressure separating plate on the lower portion, an opening which allows a corresponding stem cell collection bag to be taken and placed is formed between every two adjacent pressure separating plates when the corresponding pressure separating plate located on the upper portion ascends and keepsaway to arrive at the extreme position, and each pressure separating plate located on the upper portion is linked with the corresponding adjacent pressure separating plate located on the lower portion through the corresponding connecting mechanism. The lifting driving mechanism is used for being optionally connected with any pressure separating plate. The fixing mechanism is used for enabling therandom different pressure separating plates to be fixed and connected. A medical worker can just loosen the corresponding stem cell collection bag as required, the collection bags which do not need to be taken out can be kept in a press-fit state, and the damage is avoided.

The invention relates to a calcium phosphate ceramic active scaffold capable of regulating and controlling cell adhesion performance. According to the method, the calcium phosphate micro-nano active coating is individually designed on the surface of the calcium phosphate biological ceramic scaffold, so that the adhesion behavior of cells is accurately regulated and controlled, and the repair process of tissues is regulated and controlled. According to the preparation method, the calcium phosphate ceramic bracket can be obtained through processes such as 3D printing, a foaming method and a tabletting method, and the surface calcium-phosphorus micro-nano active coating is obtained through processes such as hydrothermal treatment, mineralization and electro-deposition. The design accurately acts on the adhesion protein of the cells at the micro-nano level, so that the change of the cell adhesion behavior is realized. According to the invention, the regulation and control effects of the material on adhesion, activity, proliferation and differentiation of cells are obviously enhanced, and the material has higher hydrophilicity, protein adsorbability, stem cell collection and tissue repair promotion capabilities.

The invention discloses a stem cell collection box, which comprises a support table. Two ends of the support table are both provided with a protective cover. Two sides of the support table are both provided with a first chute. Two first slide blocks are arranged in each first chute. Two slide blocks in the same side form a group. One side of two first blocks of a same group is fixed on the lateralwall of one end in one protective cover. A limit slot is arranged in the support table. A support box is arranged in the limit slot. A placing cavity is arranged in the support box. The lateral wallof the upper end of the placing cavity is hinged to a sealing cover. The opposite lateral walls of the placing cavity are both rotatingly connected to a telescoping rod. One end of each telescoping rod is rotatingly connected to one side of the sealing cover. Second chutes are arranged in both opposite lateral walls of the placing cavity. The collection box is fully protected, at the same time, the placement area of the collection box is enlarged, a bottle can be stably clamped and fixed, the operation is convenient, and the stem cell collection box is suitable for popularization.

The invention belongs to the technical field of medical instruments and particularly relates to a medical hematopoietic stem cell collector which comprises a mounting body, a blood collection unit, astem cell separation unit, a stem cell collection tank, a plasma collection tank, a pressurization pump, a negative pressure pump, a heparin bottle, a plasma conveying pipe and an ice block storage tank; the blood collection unit is connected with the stem cell separation unit; the stem cell separation unit is connected with the stem cell collection tank and also is connected to a plasma separation tank; the pressurization pump is connected with the plasma collection tank; the negative pressure pump is connected with the stem cell collection tank; the heparin bottle is connected to the stem cell separation unit; one end of the plasma conveying pipe is connected with the blood collection unit; the other end of the plasma conveying pipe is connected with the plasma collection tank; and the plasma conveying pipe is also provided with a normal saline introduction pipe. The medical hematopoietic stem cell collector provided by the invention can improve the purification accuracy when stem cells are collected by keeping continuous working state.

The invention belongs to a medical apparatus and relates to a filter separator of mesenchyma stem cells and an application thereof. The filter separator of the mesenchyma stem cells is composed of an inflating bulb, a degerming filter, a rubber plug, an air compression room, a conical stainless steel screening net, a stem cell collecting bottle and a stable-pressure opening. The filter separator of the mesenchyma stem cells has the main characteristics and advantages as follows: (1) all parts can be conveniently detached, washed and sterilized; (2) due to the unique design of the conical cellscreening net, the surface tension of an upper fluid is reduced, the surface area, the volume and the lower-layer pressure of the bearing liquid are increased, the liquid can favorably flow down and the filtering efficiency of the mesenchyma stem cells is obviously increased; (3) the operation time of separating and preparing the mesenchyma stem cells is shortened, the possibility of polluting isreduced and the survival rate of the mesenchyma stem cells is increased; (4) the processing amount is great, the number of the separated and prepared mesenchyma stem cells is large and the quality ofthe separated and prepared mesenchyma stem cells is good; (5) the filter separator is convenient to operate and low in price. The filter separator provided by the invention can be used for separatingand preparing the mesenchyma stem cells from human bodies or animal placentas, amnions, umbilical cords and the like and the separated and prepared mesenchyma stem cells can be used for clinical treatment or laboratory research.

The invention discloses a preparation method and application of a targeting mesenchymal stem cell (MSC) drug delivery system. The preparation method comprises the steps as follows: S1, stem cell collection; S2, cell detection; S3, production of a transfection mixed solution; S4, pretreatment of MSCs; and S6, alkaline culture. The MSCs are widely sourced, can be xenogenous as well as autogenous andhave sufficient sources, and the cells have excellent capacity in proliferation, survival, differentiation, migration, synthesis, factor secretion and other aspects, are free of ethical problems andhave low immunogenicity and broad application prospects. The disclosed tissue damage targeting MSCs can be effectively planted at tissue damage parts, blood washing or natural falling can be avoided,the homing cell quantity can be increased, besides, low expression of smad protein can be avoided, and the fibrosis promotion risk due to transplantation of the MSCs can be avoided.

The invention discloses a stem cell preparation preparing device with good sealing performance and convenient to detach and wash. The stem cell preparation preparing device comprises a lower-end connecting shell, wherein a connector is arranged at the upper end of the lower-end connecting shell; the outer side of the upper end of the connector is connected with an upper-end connecting shell through a connecting seat; a fixing plate is arranged at the lower end in the upper-end connecting shell and an annular groove is formed in the upper end of the lower-end connecting shell; a connecting ringcorresponding to the annular groove of the lower-end connecting shell is arranged on the lower surface of the fixing plate; a washing solution in a washing solution storage box can be pumped into a washing pipe through a water suction pump and a stem cell collection groove can be washed through a washing pipe; a centrifugal pipe can be driven to rotate through a centrifugal motor and the centrifugal pipe is washed, so that the centrifugal pipe is more convenient to wash; the stem cell preparation preparing device with the good sealing performance and convenient to detach and wash is simple instructure and convenient to operate; the stem cell preparation preparing device is more convenient to detach and is more convenient to wash, so that convenience is provided for people.

The invention relates to the technical field of medical instruments, and in particular relates to a stem cell thawing structure. The main technical problem to be solved is how to improve the heat exchange efficiency between a stem cell collection bag in a box body and water. The stem cell thawing structure in the invention comprises a stem cell collection bag, a box body, partition plates, a circulating pump, a heating device and a clamping assembly; a closed cavity is formed in the stem cell collection bag; furthermore, a through hole is formed in the side surface; the cavity surrounds the through hole; a flow inlet is formed in one side surface of the box body; a flow outlet is formed in the other opposite side surface of the box body; more than two partition plates are sequentially arranged between the flow inlet and the flow outlet in the box body at intervals so as to divide a fluctuating flow channel between the flow inlet and the flow outlet; the clamping assembly is used for clamping the stem cell collection bag in the flow channel; and the circulating pump is arranged outside the box body, and is connected between the flow inlet and the flow outlet. The stem cell thawing structure disclosed by the invention can realize rapid thawing of the stem cell collection bag.

A stem cell preparation system comprises a stem cell preparation device, a digestive juice box, a normal saline box, a supernatant collecting box, a waste liquid collecting box, a culture medium box,a culture medium recycling box and a stem cell collector. The stem cell preparation device comprises a shell, and a digestion chamber, a centrifugal chamber and a culture chamber which are positionedin the shell and are sequentially connected from top to bottom. The digestive juice box, the normal saline box, the supernatant collecting box, the waste liquid collecting box, the culture medium box,the culture medium recycling box and the stem cell collector are connected with the stem cell preparation device through pipelines. The stem cell culture system is high in integration level and automation degree, manual participation is not needed in the stem cell culture process, and the influence of external bacteria on stem cell culture is reduced to the maximum extent.

The invention relates to a preparation method of bacteriostatic hydrogel containing human umbilical cord mesenchymal stem cell freeze-dried powder. The preparation method comprises the following steps: firstly, culturing and incubating human umbilical cord mesenchymal stem cells, collecting supernate of the human umbilical cord mesenchymal stem cells, and freeze-drying to obtain stem cell freeze-dried powder; freeze-drying ulva powder, extracting ulva from the freeze-dried ulva powder, oxidizing to obtain dialdehyde ulva, carrying out Schiff base reaction on chitosan and dialdehyde ulva, introducing dopamine, preparing silver nanoparticles, and constructing to obtain the dialdehyde ulva / chitosan / dopamine / silver nanoparticle composite hydrogel; and finally, dissolving the human umbilical cord mesenchymal stem cell freeze-dried powder in a phosphate buffer solution to obtain a stem cell freeze-dried powder solution, and the dialdehyde ulva / chitosan / dopamine / silver nanoparticle composite hydrogel is soaked for a certain time. The composite hydrogel system disclosed by the invention can inhibit bacteria, prevent wound infection and promote accelerated healing of wounds; and the water-absorbing swelling property and the moisture retention property are good.

The invention belongs to the technical field of medical devices, specifically a medical hematopoietic stem cell collection instrument, including an installation body, a blood collection unit, a stem cell separation unit, a stem cell collection tank, a plasma collection tank, a pressurized pump, a negative pressure pump, a heparin bottle, The plasma conveying tube and the ice cube storage box, the blood collection unit is connected with the stem cell separation unit; the stem cell separation unit is connected with the stem cell collection tank; the stem cell separation unit is also connected with the plasma separation tank; the booster pump is connected with the plasma collection tank; The pressure pump is connected to the stem cell collection tank; the heparin bottle is connected to the stem cell separation unit; one end of the plasma delivery tube is connected to the blood collection unit, and the other end of the plasma delivery tube is connected to the plasma collection tank; the plasma delivery tube is also provided with a normal saline introduction tube; The invention can improve the purification precision of stem cell collection under the continuous working state.

The invention discloses a stem cell collection device in the field of stem cell collection technology, comprising a box body, the inner wall of the box body is divided into a blood storage room and a separation room through a partition, a rotating shaft is connected to the center of the top wall of the cover body, and a position above the rotating shaft is provided. Cleaning component, the bottom end of the partition is connected to the lower liquid pipe with a solenoid valve, and the bottom end of the lower liquid pipe is rotatably connected and connected to the stem cell separator; the invention adds the cleaning component, when the blood storage chamber needs to be cleaned, it can be rotated ring, so that it moves to the inside, the vertical bar and the inclined bar leave the first cavity and the second cavity respectively, turn the limit assembly, and then turn the sleeve so that the first screw rod passes through the through hole, and then lock it with the nut Tight, clean water is sprayed from the annular nozzle, and the first motor drives the cleaning component to rotate to clean the inner wall of the blood storage chamber. Under the joint action of clean water and the cleaning brush, the residual blood stains can be fully cleaned, and the cleaning effect is good; the cleaning component does not In contact with blood, avoid contamination of the cleaning brush with blood.

The invention relates to the technical field of medical equipment, and relates to a convenient medical stem cell preservation device. The medical stem cell preservation device comprises a tank, spacing and pressing plates, a lifting drive mechanism and a fixing mechanism, wherein the more than two spacing and pressing plates are arranged sequentially in the tank along the height direction, and canlift up and lower down in the tank; a connecting mechanism is arranged between each two adjacent spacing and pressing plates; in the height direction, one spacing and pressing plate located above canget close or be far away relative to the adjacent spacing and pressing plate located below; an opening for picking and placing a stem cell collection bag is formed between one spacing and pressing plate located above and the adjacent spacing and pressing plate located below when the spacing and pressing plate located above lifts up and is far away to arrive at the extreme position; the spacing and pressing plate located above is linked with the adjacent spacing and pressing plate located below through the corresponding connecting mechanism; the lifting drive mechanism is used for being optionally connected with any one spacing and pressing plate; and the fixing mechanism is used for fixedly connecting any different spacing and pressing plates. A medical worker can only loosen a corresponding stem cell collecting bag according to the needs, and collecting bags which do not need to be taken out keep at pressing states so as to be prevented from being damaged.

A stand for the collection of stem cells from a placenta. The stand includes a base with an upper surface adapted to hold a placenta during stem cell collection, a back support, and an upper platform with a surface suspended above the base and adapted to hold the placenta during collection of stem cells. The stand is preferably composed of cardboard or a similar material and is foldable between a folded configuration and an assembled configuration. The back support is hinged to the base and can move between a position parallel to the base in the folded configuration and a position perpendicular to the base in the assembled configuration.

The invention relates to the field of biotechnology and particularly relates to a menstrual blood preserving fluid and an application thereof and a separation culture method of menstrual blood-source endometrium stem cells, wherein the menstrual blood is the source of the menstrual blood-source endometrium stem cells. The menstrual blood of women is used as the source for stem cell collection, so that the waste is utilized and turned into wealth while the source is abundant; the number of endometrium stem cells in the menstrual blood is 30 times that in bone marrow, and the active ingredients are plentiful; and the menstrual blood is preserved by the menstrual blood preserving fluid so as to prevent the menstrual blood from being contaminated by the outside in a process from collection to separation. In the invention, the menstrual blood is preserved by the menstrual blood preserving fluid with a unique formula, and the contamination rate of the menstrual blood is remarkably reduced while the purity and activity of the obtained menstrual blood-source endometrium stem cells are guaranteed.

The invention relates to the technical field of medical instruments, in particular to a medical stem cell thawing apparatus. The invention mainly aims to solve technical problems in improving the heatexchange efficiency between a stem cell collection bag and water both located in a box. The medical stem cell thawing apparatus comprises a stem cell collecting bag, a box body, partition plates, a circulating pump, a heating device and a clamping assembly, wherein a closed cavity is formed in the stem cell collecting bag, a through hole is formed in the side surface of the stem cell collecting bag, and the cavity surrounds the through hole; a flow inlet is formed in one side face of the box body, and a flow outlet is formed in the other opposite side face of the box body; the two or more partition plates are sequentially arranged between the flow inlet and the flow outlet in the box body at intervals so as to divide a fluctuating flow channel between the flow inlet and the flow outlet; the clamping assembly is used for clamping the stem cell collecting bag in the flow channel; and the circulating pump is arranged on the outer side of the box body and is arranged between and connectedwith the flow inlet and the flow outlet. The medical stem cell thawing apparatus disclosed by the invention can be used for quickly thawing the stem cell collection bag.

The invention discloses a stem cell collection device, and belongs to the technical field of stem cell collection. The stem cell collection device comprises a workbench, a separation device, an extraction and collection device, a flushing device, a pushing device and a culture dish, wherein the workbench is arranged on a horizontal plane, the separation device is fixedly arranged on one side of the workbench, the extraction and collection device is arranged on the workbench and located beside the separation device, the flushing device is arranged on the workbench, the flushing end of the flushing device extends into the extraction and collection device, the pushing device is arranged on the workbench and located beside the extraction and collection device. the culture dish is arranged on the workbench and located on the opposite side of the pushing device. Through the cooperative work of the separation device, the extraction and collection device, the flushing device, the pushing device and the culture dish, the stem cell extraction and collection work can be completed.