15 results about "Stem Cell Collection" patented technology

The utility model relates to stem cell collection technical field, concretely disclose a kind of collection device for subtotipotent stem cell, including filter cartridge, the bottom of filter cartridge is fixedly installed with closure, the top of filter cartridge is fixedly installed with input pipe, the bottom of filter cartridge is fixedly installed with discharge pipe, the outside of discharge pipe is provided with sterile collection bottle, the inside of filter cartridge is provided with filter assembly, the top of input pipe is provided with fixed dismounting assembly, the top of fixed dismounting assembly is provided with connecting pipe, the top of connecting pipe is fixedly installed with collection bag, this is used for the collection device of subtotipotent stem cell, flange bolt connection and pipe clamp sealing design are used in fixed dismounting assembly, both through rigid connection ensure pipeline stable, and sterile environment is formed using sealing washer to prevent pollution, and support quick dismounting replacement collection bag, and give consideration to sealing property, convenience and reuse requirement.

The invention discloses a stem cell collection and extraction device, belongs to the technical field of cell separation and extraction, and aims to solve the problems of complex and dispersed operation procedures, need of cooperation of a plurality of workers, investment of a large amount of manpower cost, low time efficiency, and high time efficiency caused by repeated secondary separation of a precipitation solution. In order to solve the problem that stem cells are mechanically damaged to a certain extent in the prior art, the stem cell extraction device comprises a working case and a discharging mechanism, the discharging mechanism is used for efficient extraction and arranged on the periphery of the bottom of a matching mechanism, and through the discharging mechanism, the continuous action of multiple sets of cyclone separation structures can be achieved; stem cells can be separated from other substances more effectively, a plurality of groups of cyclone separation structures can perform multiple times of fractional separation on liquid in the centrifugal process, and each stage of cyclone separator can perform primary separation and enrichment on components in the liquid, so that the stem cells can reach higher purity within a shorter time; and the activity and function of the stem cells can be maintained.

The utility model relates to the technical field of stem cell collection and extraction devices, in particular to an umbilical cord mesenchymal stem cell collection and extraction device which is characterized in that a stirring mechanism is started to stir crushed umbilical cord tissues and a stem cell decomposition solution, so that stem cells in the umbilical cord tissues are fully decomposed, and the umbilical cord mesenchymal stem cells are collected and extracted. The stem cell solution is discharged into the separating mechanism through the discharging mechanism, stem cells in the stem cell solution are separated by starting the separating mechanism, the solution is discharged through the discharging mechanism, and the practicability of the equipment is improved; comprising a feeding mechanism; the device further comprises a crushing mechanism, a stirring mechanism, a separating mechanism, a discharging mechanism and a cleaning mechanism, the crushing mechanism and the stirring mechanism are both installed in the feeding mechanism, the stirring mechanism is located below the crushing mechanism, the discharging mechanism is located on the right side of the feeding mechanism and installed in the separating mechanism, and the cleaning mechanism is installed on the left side of the feeding mechanism.

The utility model discloses transportation equipment for stem cell collection, and belongs to the technical field of medical treatment. The device mainly comprises a transport box, a containing groove is formed in the upper side of the transport box, a box cover is rotationally arranged on one side of the upper end of the containing groove, a putting-in opening is formed in one side of the interior of the containing groove and communicates with the outer side of the transport box, and a cover plate is rotationally arranged on one side of the outer end of the putting-in opening; a guide rail is connected between the end, away from the placing opening, of the bottom of the containing groove and the end, close to the placing opening, of the bottom of the containing groove, a plurality of containing boxes are placed in the containing groove, a test tube hole is formed in one end of each containing box, and a sliding groove matched with the guide rail is formed in the bottom of each containing box. According to the transportation equipment for stem cell collection, the effect of reducing temperature changes in the transportation box when test tubes are stored is achieved.

The invention belongs to the technical field of medical instruments, and provides a stem cell collecting device which comprises a base, a supporting frame, a rotating mechanism and a motor. The lower end of the supporting frame is connected with the base, the slewing mechanism is rotationally arranged on the base, the motor is arranged at the upper end of the supporting frame, the output end of the motor is connected with the upper end of the slewing mechanism, the motor can drive the slewing mechanism to rotate, and the upper portion of the slewing mechanism can fix the umbilical cord and enable the umbilical cord to be in a vertical state. A blood collection bag can be fixed to the lower portion of the swing mechanism. The umbilical cord and the blood taking bag are fixed through the rotating mechanism, the umbilical cord is in the vertical state, the blood taking bag and the lower end of the umbilical cord are communicated through the blood taking needle, blood in the umbilical cord is gathered downwards under the gravity effect and then flows into the blood taking bag through the blood taking needle, on one hand, the blood flowing speed can be increased, and the blood taking efficiency is improved; on the other hand, blood in the umbilical cord can be conveyed into the blood collection bag as much as possible, the blood collection amount is increased, and the utilization rate of the blood in the umbilical cord is increased.

The invention provides a method for separating and culturing olfactory mucosa stem cells, which comprises the following steps of: firstly, collecting and cleaning 0.5-3mm < 3 > tissue blocks from an olfactory region, then carrying out enzymolysis treatment, releasing high-activity stem cells, filtering and centrifuging at a low speed, pre-culturing a cell suspension in a serum-free culture medium with the serum concentration of less than or equal to 2 percent for 6-12 hours so as to inhibit parenchyma cells from adhering to the wall, and then, separating and culturing the parenchyma cells to obtain the olfactory mucosa stem cells. Adding a specific magnetic bead probe to incubate for 5-30 minutes, applying an external magnetic field to remove miscellaneous cells with magnetic beads, collecting target stem cells, amplifying the collected cells in a culture medium containing EGF and PDGF, carrying out passage or cryopreservation when the fusion degree of the cells reaches 80-90%, and during cryopreservation, carrying out cryopreservation on the 2nd-4th generation cells in a cryopreservation solution containing 90% of human serum albumin and 10% of DMSO, the temperature is reduced by 1-80 DEG C per minute, then liquid nitrogen preservation is carried out, through two-step enzyme digestion, fibroblasts and epithelial cells are firstly removed, then olfactory mucosa stem cells are accurately released, and enzyme damage is reduced.

The utility model relates to a visible low-temperature preservation bag of stem cells and an incubator, the visible low-temperature preservation bag of stem cells comprises a bag body, a cold source cavity and an opening binding part, the bag body is a transparent bag body or a visible area is arranged on the side wall of the bag body, and a storage area for placing a stem cell collection bag is arranged in the bag body; the cold source cavity comprises a first cold source cavity arranged at the bottom of the inner side of the bag body and a second cold source cavity arranged on the inner side wall of the bag body and communicated with the first cold source cavity; the opening binding part is arranged at the top opening of the bag body. The stem cell heat preservation box comprises a box body, a plurality of subareas arranged in the box body and used for storing the stem cell visual low-temperature preservation bags and a cover, heat preservation materials are laid on the inner side walls and the bottoms of the subareas, a handle is arranged on the top of the cover, and a second label indication area is arranged on the outer wall of the box body. According to the utility model, the timely low-temperature preservation after the cells are collected can be met, and a new cold source can be replaced at any time by observing the temperature in the stem cell visible low-temperature preservation bag and the cell state, so that the cells are kept in a low-temperature environment, and the cell preservation time is prolonged.

The utility model discloses an anti-toppling stem cell collection kit which comprises a kit body, a groove is formed in the top of the kit body, a containing groove is formed in the bottom of the groove, a sliding groove is formed in the inner side of the containing groove, a sliding plate is installed in the sliding groove, a clamping plate is fixed to the position, located in the containing groove, of one side of the sliding plate, and a clamping groove is formed in the other side of the sliding plate. A first rubber sheet is fixed to the inner side of the clamping plate, and a second rubber sheet is fixed to the position, away from the first rubber sheet, of the inner side of the containing groove. According to the anti-toppling stem cell collection kit, by rotating the handle, the multiple sliding plates and the clamping plate can move rightwards at the same time, test tubes are clamped and fixed, and the working efficiency is improved; the test tubes cannot topple over, the stability of the fixed test tubes is improved, the test tubes are protected, the height of the clamping plates is four fifths of the height of the test tubes, the contact area between the clamping plates and the test tubes is increased, and the stability of test tube fixing is further improved.

The utility model discloses a transport box for stem cell collection, which comprises a heat preservation box and a spring shock absorber, support arms are fixed on the outer walls of the lower ends of the two end faces of the heat preservation box, support sleeves are sleeved on the outer walls of the support arms, a heat preservation cover is hinged to the upper end of the heat preservation box, and a sealing gasket is adhered to the lower surface of the heat preservation cover. A positioning plate is fixedly arranged on the inner wall of the heat preservation box, grid plates are fixed to the positions, on the two sides of the positioning plate, of the inner wall of the heat preservation box, tooth protrusions are fixedly arranged on the outer wall of an adjusting sleeve, a lifting rod is installed on the inner wall of the adjusting sleeve in a threaded mode, a locking button is installed on the inner wall of the upper end of a locking plate in a threaded mode, and a tooth frame is rotationally installed at the tail end of the locking button. The transport box for the stem cell collected objects can be conveniently adjusted according to the heights of collected object bottles, so that the bottles with different heights can be conveniently and stably fixed in the device to be transported, and the phenomenon that the bottles collide with the device and are damaged is avoided.

The invention belongs to the technical field of cell sorting, and particularly relates to a preparation and preservation method of a stem cell source exosome. The preparation method comprises the following steps: cutting umbilical cord tissues into small blocks, adding digestive juice containing collagenase I and hyaluronidase for digestion, and collecting tissue precipitates for culture to obtain cell culture fluid; centrifuging the cell culture solution, collecting cell precipitates, resuspending, adding fluorophore coupled positive antibodies and / or negative antibodies to mark the human umbilical cord mesenchymal stem cells, and sorting the human umbilical cord mesenchymal stem cells; the method comprises the following steps: culturing human umbilical cord mesenchymal stem cells, collecting a stem cell culture solution, and extracting exosomes in the stem cell culture solution through a differential centrifugation method. The invention provides a large-scale and reliable exosome preparation scheme for clinical application, provides powerful guarantee for long-term storage and transportation of the exosome and wide application in clinical application and scientific research, and has important scientific significance and wide market prospect.

The utility model provides a storage device for stem cell collection, and relates to the field of stem cell storage, the storage device comprises a storage unit, one side of the storage unit is provided with a refrigeration unit, the storage unit comprises a storage box, and an inner cavity of the storage box is movably connected with a placing cylinder; the pressing plate can be driven to move downwards to be in contact with the cell bottle through the reset elastic force of the spring, then the cell bottle can be positioned, the stability of later storage of the cell bottle is ensured, liquid nitrogen in the liquid nitrogen tank is conveyed to the inner cavity of the hose and the spray head, the liquid nitrogen is conveyed to the interior of the storage box through the spray head, and then the interior of the storage box can be cooled. The rotating disc drives the screw rod to rotate, so that the screw rod drives the sleeve plate to move upwards, the sleeve plate drives the plugging disc, the placing barrel and the cell bottles to move upwards, the placing barrel and the cell bottles are gradually separated from the inner cavity of the storage box, then the cell bottles are conveniently taken out, people do not need to stretch hands into the storage box to take out the cell bottles, and the safety is improved.

The utility model discloses a stem cell collecting device with a protective structure, which relates to the technical field of stem cell collection and comprises a base, a mounting plate is fixedly mounted on the upper side of the base, a shaking component is rotatably mounted on the surface of the mounting plate, an L-shaped plate is rotatably mounted on the mounting plate through the shaking component, and a protective cover is mounted on the L-shaped plate. The device comprises an L-shaped plate, the inner bottom wall of the L-shaped plate is fixedly provided with a bottle sleeve, one side of the L-shaped plate is provided with an adjusting groove, and a fixing assembly used for fixing a collection bottle is installed in the adjusting groove in a sliding mode. The two adjusting blocks drive the two clamping blocks to get close to each other respectively, then an inserting rod is inserted into an inserting hole, and an adjusting knob is locked, so that a bidirectional lead screw is locked, the effect of fixing a collecting pipe is enhanced, and the problem that the collecting pipe is loosened in the shaking process is solved.

The utility model discloses stem cell collection and extraction equipment, and relates to the technical field of stem cell collection, and the stem cell collection and extraction equipment comprises a centrifugal machine, the centrifugal machine comprises a machine box, a working chamber is arranged in the middle of the top surface of the machine box, and a rotating seat is rotatably arranged in the bottom surface of the working chamber; a driving shaft is fixedly installed in the middle of the bottom face of the rotating seat, an extracting assembly is fixedly installed on the top face of the rotating seat, under the cooperation of the positioning assembly and the positioning piece, storage barrels for storing reagent tubes can be conveniently disassembled and cleaned, and the storage barrels with different inner tube diameters are installed through the positioning piece, so that the efficiency is improved. The preparation work of storing cell extracting solutions in reagent tubes with different tube diameters is completed, four supporting tables are installed and fixed through positioning sleeves, positioning of the bottom faces of the corresponding storage barrels is completed through limiting grooves formed in one sides of the top faces of the supporting tables, and the stability of the storage barrels during operation is improved.

The invention discloses a preparation method and application of a cryopreservation solution supporting high-density clinical stem cells. The cryopreservation liquid is composed of normal saline injection, human serum albumin (HSA) for injection, glucose, dimethyl sulfoxide (DMSO) for injection, hydroxyethyl starch injection, dextran injection and the like. And the high-density clinical stem cell cryopreservation liquid is prepared through accurate proportioning under the sterile condition. The application method comprises the following steps: after collecting stem cells, re-suspending and uniformly mixing the stem cells with a cryopreservation solution pre-cooled to 4 DEG C, slowly cooling to-80 DEG C by a program, and then transferring the stem cells into liquid nitrogen for long-term preservation. During resuscitation, the temperature is rapidly increased to 37 DEG C, so that the cell cryopreservation liquid is rapidly thawed, and then the cell cryopreservation liquid is recovered to room temperature and can be directly used for clinical local injection or used for intravenous drip after being diluted by normal saline.

This disclosure relates to the field of cell collection technology. One embodiment of this disclosure provides a stem cell collection device, which includes: a blood collection tube and a shell, the blood collection tube being located inside the shell; a suction assembly being disposed inside the blood collection tube and the shell; a cooling assembly being disposed on the blood collection tube; the suction assembly includes a blood collection needle disposed at the top of the blood collection tube; the top of the shell has several slots; several inserts are provided on the shell, the inserts being inserted into the slots; and the upper end of the blood collection tube has a first threaded groove. This technical solution solves the problem in the prior art where the amount of blood collected by a vacuum blood collection tube is fixed, while different amounts need to be collected for different types of blood.