45 results about "Stem Cell Collection" patented technology

The present invention provides improved compositions and methods for the collection of stem cells from an organ, e.g., placenta. The invention provides a stem cell collection composition comprising an apoptosis inhibitor and, optionally, an enzyme such as a protease or mucolytic enzyme, vasodilator, necrosis inhibitor, oxygen-carrying perfluorocarbon, or an organ preserving compound. The invention provides methods of using the stem cell collection composition to collect stem cells and to preserve populations of stem cells.

The invention provides a separation and purification method of a human umbilical cord mesenchymal stem cell exosome and an application of the human umbilical cord mesenchymal stem cell exosome, and belongs to the technical field of medicines. The human umbilical cord mesenchymal stem cell exosome provided by the invention is prepared by cultivating human umbilical cord mesenchymal stem cells via aserum-free medium, collecting supernatant liquid, implementing centrifuging as well as ultra-filtration and concentration, transferring a concentrated solution onto a 30% sucrose-heavy water densitypad and implementing further purification through sucrose density centrifuging, so that the human umbilical cord mesenchymal stem cell exosome is obtained. According to the separation and purificationmethod that the human umbilical cord mesenchymal stem cell exosome is obtained through separation and purification, immunoreactivity is effectively reduced, and a controllable dosage when the human umbilical cord mesenchymal stem cell exosome is used is guaranteed. The human umbilical cord mesenchymal stem cell exosome, by improving a degree of activating an insulin signaling pathway of a type IIdiabetes animal model, can inhibit composition and decomposition of hepatic glycogen, so that glucose metabolism homeostasis can be achieved; and meanwhile, the sensitivity of the type II diabetes animal model to insulin and an insulin secretion function of pancreatic [beta] cells can be improved and a blood glucose concentration can be reduced, so that the application of the human umbilical cordmesenchymal stem cell exosome to the preparation of medicines for treating type II diabetes can be achieved.

The subject matter of this invention is a novel method to augment the process of obtaining populations of placental hematopoietic stem and progenitor cells for use in medical practices. A method of augmentation of stem cells collection from placenta is claimed comprising the steps of (a) infusing placental vessels cell preservation compound and a with a composition containing blockers of cell adhesion receptors, (b) incubating said placenta for a sufficient period of time, (c) placing placenta in a containment with sufficient intensity of electromagnetic or ultrasound field for a sufficient period of time; (d) eluting cell suspension from placental vessels, (e) collecting cell suspension and harvesting cells. Invention further claims a method of treatment a disease by means of administering therapeutic composition containing said placental-derived hematopoietic stem cells.

Apparatus and methods for collecting stem cells from an umbilical cord utilize a plurality of needles that are inserted into the umbilical cord. The needles may be fenestrated, and may have a common output to a line via which the stem cells are collected. A negative pressure source, such as a vacuum, may be used to draw the cells through the needles and the line. Apparatus for holding the umbilical cord, compressing the umbilical cord, positioning the plurality of needles in relation to the umbilical cord, guiding insertion of the needles toward and into the umbilical cord, withdrawing blood from the umbilical cord, and/or collecting blood withdrawn from the umbilical cord may be provided. One or more one-way check valves may be used to control fluid flow. Additionally or alternatively, a shunt may be used during blood collection to prevent backflow through the needles.

The invention provides a method and system for separating, culturing and amplifying human umbilical cord mesenchymal stem cells. The method comprises the following steps: step I, pretreating a sample; step II, obtaining Wharton's jelly, cutting the obtained Wharton's jelly into small tissue, and adding a filtered red blood cell lysis buffer solution of which the volume is 3.5 times that of the small tissues; step III, digesting collagenase, wherein a digestion solution is a balanced salt solution containing 1% of IV type collagenase, 0.5% of hyaluronidase, 300U/ml DNA enzyme and 2% of a serum substitute; step IV, performing primary cell isolated culture: adding a serum-free culture medium of mesenchymal stem cells to obtain a cell suspension, and adding a special primary cell culture solution for umbilical cord mesenchymal stem cells for primary culture; step V, subculturing the cells, and inoculating the collected cells according to 5000 cells/cm < 2 > by using trypsin with the mass percent concentration of 0.215%; and step VI, collecting the umbilical cord mesenchymal stem cells, and performing osteogenic differentiation, adipogenic differentiation and chondrogenic differentiation experiments.

The invention discloses an upper limb supporting frame assembly for stem cell collection, and relates to the technical field of medical stents. The upper limb supporting frame assembly comprises an upper limb supporting part, wherein the upper limb supporting part comprises a length-adjustable forearm arc-shaped plate; one end of the forearm arc-shaped plate is rotationally connected with a hand arc-shaped plate; the bottom of the other end of the forearm arc-shaped plate is rotationally connected with a universal joint; and the movable end of the universal joint is fixedly connected with a shoulder arm arc-shaped plate. The upper limb supporting frame assembly has a reasonable structure, the angle and the position of each joint of the upper limb supporting frame part can be adjusted to meet the optimal comfort of a patient, the arm arc-shaped plate is bent at an angle relative to an armpit supporting block, arm and shoulder arm bending of the patient is simulated, and the forearm arc-shaped plate rotates and swings relative to the armpit supporting block to simulate a situation that the arm of the patient rotates and swings relative to the shoulder arm. Therefore, the normal movement operation of the upper limbs of the patient is simulated, pain and numbness caused by long-time immobility of the upper limbs of the patient are relieved, and comfort of the human body is achieved.

The invention relates to the technical field of medical equipment, and relates to a device for preserving umbilical cord blood stem cells. The device comprises a box body, pressure separating plates,a lifting driving mechanism and a fixing mechanism. The two or more pressure separating plates are arranged, and all the pressure separating plates are arranged in the box body in sequence in the height direction and can ascend and descend in the box body. A connecting mechanism is arranged between every two adjacent pressure separating plates. In the height direction, each pressure separating plate located on the upper portion can relatively get close to or keep away from the corresponding adjacent pressure separating plate on the lower portion, an opening which allows a corresponding stem cell collection bag to be taken and placed is formed between every two adjacent pressure separating plates when the corresponding pressure separating plate located on the upper portion ascends and keepsaway to arrive at the extreme position, and each pressure separating plate located on the upper portion is linked with the corresponding adjacent pressure separating plate located on the lower portion through the corresponding connecting mechanism. The lifting driving mechanism is used for being optionally connected with any pressure separating plate. The fixing mechanism is used for enabling therandom different pressure separating plates to be fixed and connected. A medical worker can just loosen the corresponding stem cell collection bag as required, the collection bags which do not need to be taken out can be kept in a press-fit state, and the damage is avoided.

The invention discloses a stem cell collection box, which comprises a support table. Two ends of the support table are both provided with a protective cover. Two sides of the support table are both provided with a first chute. Two first slide blocks are arranged in each first chute. Two slide blocks in the same side form a group. One side of two first blocks of a same group is fixed on the lateralwall of one end in one protective cover. A limit slot is arranged in the support table. A support box is arranged in the limit slot. A placing cavity is arranged in the support box. The lateral wallof the upper end of the placing cavity is hinged to a sealing cover. The opposite lateral walls of the placing cavity are both rotatingly connected to a telescoping rod. One end of each telescoping rod is rotatingly connected to one side of the sealing cover. Second chutes are arranged in both opposite lateral walls of the placing cavity. The collection box is fully protected, at the same time, the placement area of the collection box is enlarged, a bottle can be stably clamped and fixed, the operation is convenient, and the stem cell collection box is suitable for popularization.

The invention discloses a preparation method and application of a targeting mesenchymal stem cell (MSC) drug delivery system. The preparation method comprises the steps as follows: S1, stem cell collection; S2, cell detection; S3, production of a transfection mixed solution; S4, pretreatment of MSCs; and S6, alkaline culture. The MSCs are widely sourced, can be xenogenous as well as autogenous andhave sufficient sources, and the cells have excellent capacity in proliferation, survival, differentiation, migration, synthesis, factor secretion and other aspects, are free of ethical problems andhave low immunogenicity and broad application prospects. The disclosed tissue damage targeting MSCs can be effectively planted at tissue damage parts, blood washing or natural falling can be avoided,the homing cell quantity can be increased, besides, low expression of smad protein can be avoided, and the fibrosis promotion risk due to transplantation of the MSCs can be avoided.

The invention discloses a stem cell preparation preparing device with good sealing performance and convenient to detach and wash. The stem cell preparation preparing device comprises a lower-end connecting shell, wherein a connector is arranged at the upper end of the lower-end connecting shell; the outer side of the upper end of the connector is connected with an upper-end connecting shell through a connecting seat; a fixing plate is arranged at the lower end in the upper-end connecting shell and an annular groove is formed in the upper end of the lower-end connecting shell; a connecting ringcorresponding to the annular groove of the lower-end connecting shell is arranged on the lower surface of the fixing plate; a washing solution in a washing solution storage box can be pumped into a washing pipe through a water suction pump and a stem cell collection groove can be washed through a washing pipe; a centrifugal pipe can be driven to rotate through a centrifugal motor and the centrifugal pipe is washed, so that the centrifugal pipe is more convenient to wash; the stem cell preparation preparing device with the good sealing performance and convenient to detach and wash is simple instructure and convenient to operate; the stem cell preparation preparing device is more convenient to detach and is more convenient to wash, so that convenience is provided for people.

The invention relates to the technical field of medical instruments, and in particular relates to a stem cell thawing structure. The main technical problem to be solved is how to improve the heat exchange efficiency between a stem cell collection bag in a box body and water. The stem cell thawing structure in the invention comprises a stem cell collection bag, a box body, partition plates, a circulating pump, a heating device and a clamping assembly; a closed cavity is formed in the stem cell collection bag; furthermore, a through hole is formed in the side surface; the cavity surrounds the through hole; a flow inlet is formed in one side surface of the box body; a flow outlet is formed in the other opposite side surface of the box body; more than two partition plates are sequentially arranged between the flow inlet and the flow outlet in the box body at intervals so as to divide a fluctuating flow channel between the flow inlet and the flow outlet; the clamping assembly is used for clamping the stem cell collection bag in the flow channel; and the circulating pump is arranged outside the box body, and is connected between the flow inlet and the flow outlet. The stem cell thawing structure disclosed by the invention can realize rapid thawing of the stem cell collection bag.

A stem cell preparation system comprises a stem cell preparation device, a digestive juice box, a normal saline box, a supernatant collecting box, a waste liquid collecting box, a culture medium box,a culture medium recycling box and a stem cell collector. The stem cell preparation device comprises a shell, and a digestion chamber, a centrifugal chamber and a culture chamber which are positionedin the shell and are sequentially connected from top to bottom. The digestive juice box, the normal saline box, the supernatant collecting box, the waste liquid collecting box, the culture medium box,the culture medium recycling box and the stem cell collector are connected with the stem cell preparation device through pipelines. The stem cell culture system is high in integration level and automation degree, manual participation is not needed in the stem cell culture process, and the influence of external bacteria on stem cell culture is reduced to the maximum extent.

The invention relates to a preparation method of bacteriostatic hydrogel containing human umbilical cord mesenchymal stem cell freeze-dried powder. The preparation method comprises the following steps: firstly, culturing and incubating human umbilical cord mesenchymal stem cells, collecting supernate of the human umbilical cord mesenchymal stem cells, and freeze-drying to obtain stem cell freeze-dried powder; freeze-drying ulva powder, extracting ulva from the freeze-dried ulva powder, oxidizing to obtain dialdehyde ulva, carrying out Schiff base reaction on chitosan and dialdehyde ulva, introducing dopamine, preparing silver nanoparticles, and constructing to obtain the dialdehyde ulva/chitosan/dopamine/silver nanoparticle composite hydrogel; and finally, dissolving the human umbilical cord mesenchymal stem cell freeze-dried powder in a phosphate buffer solution to obtain a stem cell freeze-dried powder solution, and the dialdehyde ulva/chitosan/dopamine/silver nanoparticle composite hydrogel is soaked for a certain time. The composite hydrogel system disclosed by the invention can inhibit bacteria, prevent wound infection and promote accelerated healing of wounds; and the water-absorbing swelling property and the moisture retention property are good.

The invention relates to the technical field of medical equipment, and relates to a convenient medical stem cell preservation device. The medical stem cell preservation device comprises a tank, spacing and pressing plates, a lifting drive mechanism and a fixing mechanism, wherein the more than two spacing and pressing plates are arranged sequentially in the tank along the height direction, and canlift up and lower down in the tank; a connecting mechanism is arranged between each two adjacent spacing and pressing plates; in the height direction, one spacing and pressing plate located above canget close or be far away relative to the adjacent spacing and pressing plate located below; an opening for picking and placing a stem cell collection bag is formed between one spacing and pressing plate located above and the adjacent spacing and pressing plate located below when the spacing and pressing plate located above lifts up and is far away to arrive at the extreme position; the spacing and pressing plate located above is linked with the adjacent spacing and pressing plate located below through the corresponding connecting mechanism; the lifting drive mechanism is used for being optionally connected with any one spacing and pressing plate; and the fixing mechanism is used for fixedly connecting any different spacing and pressing plates. A medical worker can only loosen a corresponding stem cell collecting bag according to the needs, and collecting bags which do not need to be taken out keep at pressing states so as to be prevented from being damaged.

A stand for the collection of stem cells from a placenta. The stand includes a base with an upper surface adapted to hold a placenta during stem cell collection, a back support, and an upper platform with a surface suspended above the base and adapted to hold the placenta during collection of stem cells. The stand is preferably composed of cardboard or a similar material and is foldable between a folded configuration and an assembled configuration. The back support is hinged to the base and can move between a position parallel to the base in the folded configuration and a position perpendicular to the base in the assembled configuration.