Edible and medicinal composition for dispelling effects of alcohol and protecting liver as well as preparation method and application of edible and medicinal composition

Abstract

The invention discloses an edible and medicinal composition for dispelling effects of alcohol and protecting liver as well as a preparation method and application thereof, and belongs to the technical field of functional foods. The composition consists of the following raw materials in parts by weight: 1-8 parts of ampelopsis grossedentata extract freeze-dried powder, 1-8 parts of astragalus membranaceus extract freeze-dried powder, 1-5 parts of kudzu vine root extract freeze-dried powder, 1-8 parts of hawthorn extract freeze-dried powder and 1-5 parts of cassia seed extract freeze-dried powder. The composition is designed according to a multi-component synergistic interaction principle, and the functions of protecting the liver and dispelling the effects of alcohol are enhanced through targeted interaction of active ingredients such as ampelopsis grossedentata flavone, puerarin and astragaloside; the compound preparation has the compound effects of protecting the liver, dispelling the effects of alcohol, resolving dampness, resisting oxidative stress, regulating lipid metabolism and the like, can remarkably relieve the problems of acute and chronic liver injury and metabolic disorder caused by alcohol intake, and meets the food-grade safety standard and the medicinal activity requirement; the important application prospect is realized in the aspect of preparing a preparation for relieving alcoholic liver injury and improving the liver detoxification function.

Description

Technical Field

[0001] The present invention belongs to the technical field of functional foods, and particularly relates to a food and drug dual-purpose hangover and liver protection composition, a preparation method and an application thereof. Background Art

[0002] The history of Chinese wine-making can be traced back thousands of years, and the culture of drinking is deeply rooted in Chinese civilization. According to the "Huangdi Neijing", "Wine is the head of all medicines", and ancient doctors have long recognized the unique properties of alcohol and its effects on the human body. However, long-term excessive drinking or alcoholism easily leads to an increased burden on the liver, causing serious health problems such as liver damage, fatty liver, alcoholic hepatitis and even cirrhosis. Modern research shows that ethanol metabolism mainly depends on liver function, and excessive drinking will interfere with the normal physiological activities of the liver, causing oxidative stress, inflammatory reactions and cell damage.

[0003] In traditional Chinese health preservation culture, although drinking is regarded as an important part of social etiquette and food culture, the ancients also attached great importance to post-drinking conditioning and liver protection methods. Ancient medical books such as the "Qianjin Yaofang" recorded various hangover and liver protection methods mainly based on herbal plants, emphasizing the use of natural therapies to balance the negative effects of alcohol on the body. However, the fast-paced modern life has increased the frequency of drinking, and many people have the habit of "drinking a large amount" of alcohol, resulting in liver damage problems that cannot be ignored.

[0004] After alcohol enters the human body, it is quickly absorbed into the blood by the gastrointestinal tract, and about 90% is metabolized by the liver. Its pharmacokinetic characteristics are as follows: under the catalysis of alcohol dehydrogenase (ADH), it generates the toxic intermediate acetaldehyde, which is then converted into acetic acid under the action of aldehyde dehydrogenase (ALDH). Among them, the Zn 2+ cofactor of ADH and the Cys302 active site of ALDH respectively constitute the key catalytic centers of the two enzymes. The accumulation of acetaldehyde not only directly damages the mitochondrial function of hepatocytes through lipid peroxidation reactions, but also triggers the activation of the NF-κB inflammatory pathway, leading to the pathological process of alcoholic liver disease (ALD).

[0005] At present, hangover and liver protection preparations mainly focus on the dual-enzyme activity regulation strategy of ADH / ALDH, but there are three major technical bottlenecks: (1) relying on exogenous chemical synthetic substances, resulting in adverse reactions such as metabolic acidosis; (2) the imbalance in the proportion of medicine and food homologous components, making it difficult to achieve the synergistic effect of multiple components; (3) ignoring the theory of meridian tropism in traditional Chinese medicine, lacking systematic compatibility, and failing to fully exert the overall efficacy of traditional Chinese medicine.

[0006] Regarding the metabolic toxicity problems caused by chemically synthesized anti - hangover agents, the existing technologies have turned to natural component synergy strategies. For example, a traditional Chinese medicine composition for relieving hangover and protecting the liver disclosed in Chinese Patent CN 115845011 A uses multiple components such as Pueraria lobata, Hovenia dulcis, Tibetan tea, Imperata cylindrica, mulberry, and licorice as raw materials, formulates the prescription according to the characteristics of the drugs, reduces the accumulation of acetaldehyde in the liver by increasing the activity of aldehyde dehydrogenase, and achieves the purpose of protecting the liver while relieving hangover. However, this technology also has certain risks. The composition of this traditional Chinese medicine composition is complex, and the network interaction of pharmacodynamic substances caused by the multi - component system may inhibit some key metabolic enzyme subtypes, resulting in an increase in the risk coefficient of drug - drug interactions.

[0007] Based on the above - mentioned pathological mechanisms and technical pain points, there is an urgent need in the field to develop a new type of anti - hangover and liver - protecting solution that conforms to the principle of component simplicity design. By strengthening the functional targeting of core active ingredients, the metabolic interference bottleneck of traditional compound preparations is broken through on the premise of ensuring biological safety, providing an innovative solution for the prevention and treatment of alcoholic liver injury. Summary of the Invention

[0008] Aiming at the above - mentioned problems, the present invention aims to provide a food - drug dual - purpose anti - hangover and liver - protecting composition and a preparation method. Specifically, it scientifically combines five medicinal materials, namely Ampelopsis grossedentata, Pueraria lobata, Astragalus membranaceus, Crataegus pinnatifida, and Cassia obtusifolia, to enhance the liver - protecting and hangover - relieving functions through a multi - target synergistic action mechanism. This compound preparation has compound effects such as protecting the liver, relieving hangover and dampness, antioxidative stress, and regulating lipid metabolism, can significantly alleviate the acute and chronic liver injuries and metabolic disorders caused by alcohol intake, and at the same time meet the food - grade safety standards and medicinal activity requirements.

[0009] In order to achieve the above - mentioned purpose, the technical solution adopted by the present invention is as follows: A food - drug dual - purpose anti - hangover and liver - protecting composition, the raw materials of the composition are calculated by weight, including: 1 - 8 parts of freeze - dried powder of Ampelopsis grossedentata extract, 1 - 8 parts of freeze - dried powder of Astragalus membranaceus extract, 1 - 5 parts of freeze - dried powder of Pueraria lobata extract, 1 - 8 parts of freeze - dried powder of Crataegus pinnatifida extract, and 1 - 5 parts of freeze - dried powder of Cassia obtusifolia extract.

[0010] Preferably, when preparing the composition, 4 parts of freeze - dried powder of Ampelopsis grossedentata extract, 5 parts of freeze - dried powder of Astragalus membranaceus extract, 5 parts of freeze - dried powder of Pueraria lobata extract, 8 parts of freeze - dried powder of Crataegus pinnatifida extract, and 2 parts of freeze - dried powder of Cassia obtusifolia extract are selected.

[0011] The efficacy manifestations of the five raw materials selected for preparing the composition are as follows:

[0012] Tengcha is cool in nature, sweet and slightly bitter in taste, and belongs to the lung and liver meridians. It has the effects of clearing heat and detoxifying, and promoting diuresis and reducing swelling. "Supplementary Records of Compendium of Materia Medica" records that it "clears the fire of the six meridians". Modern research shows that it is rich in flavonoids such as dihydromyricetin, which can inhibit lipid peroxidation, reduce the toxicity of acetaldehyde, the metabolite of ethanol, to liver cells, and accelerate the decomposition of ethanol, reducing the metabolic burden on the liver. Its mechanism of relieving alcohol and protecting the liver lies in antioxidant stress and regulating the activity of alcohol dehydrogenase.

[0013] Kudzu root is cool in nature, sweet, pungent and bitter in taste, and belongs to the spleen and stomach meridians. It has the functions of relieving muscle fever and promoting the production of body fluid to quench thirst. "Shennong Ben Cao Jing" says that it "maintains fluid metabolism and relieves various toxins". The puerarin and daidzein it contains can inhibit the production of acetaldehyde, activate alcohol dehydrogenase and acetaldehyde dehydrogenase, accelerate ethanol metabolism, and reduce blood alcohol concentration. Its liver-protecting effect is reflected in reducing the mitochondrial damage of liver cells induced by ethanol, inhibiting the activation of hepatic stellate cells, and slowing down the process of liver fibrosis.

[0014] Cassia seed is slightly cold in nature, sweet, bitter and salty in taste, and belongs to the liver and large intestine meridians. It is good at clearing the liver and improving eyesight, and moistening the intestines and promoting defecation. "Yaoxing Lun" records that it "benefits the five internal organs and removes the heat of the liver". The anthraquinones such as chrysophanol and cassia seed pigment it contains can enhance the activity of liver detoxifying enzymes, promote bile excretion, accelerate the clearance of alcohol metabolites, and reduce the oxidative damage and lipid accumulation of liver cells induced by ethanol.

[0015] Astragalus membranaceus is warm in nature, sweet in taste, and belongs to the spleen and lung meridians. It has the effects of replenishing qi and raising yang, and consolidating the exterior and promoting diuresis. "Huayan Biyan" says that it "tonifies the lung and spleen, strengthens the exterior and restrains sweating". Astragaloside IV and polysaccharide components can up-regulate the expression of superoxide dismutase in liver cells, reduce the inflammatory reaction and fibrosis process in alcoholic liver injury. Its liver-protecting effect is achieved by enhancing the regenerative ability of liver cells and regulating the Th1 / Th2 immune balance.

[0016] Hawthorn is slightly warm in nature, sour and sweet in taste, and belongs to the spleen, stomach and liver meridians. It is good at promoting digestion and resolving food stagnation, and promoting qi circulation and removing blood stasis. "Supplementary Records of Compendium of Materia Medica" says that it "strengthens the stomach and promotes qi circulation". Ursolic acid and hyperoside it contains can activate the AMPK pathway, inhibit the expression of fatty acid synthase, and reduce lipid deposition in alcoholic fatty liver. Its mechanism of relieving alcohol is related to promoting the decomposition of ethanol into acetic acid and accelerating the activity of acetaldehyde dehydrogenase, while improving liver microcirculation disorders.

[0017] The preparation method of the above-mentioned food and drug dual-purpose composition for relieving alcohol and protecting the liver is as follows:

[0018] 1) Weigh 2 - 5 parts of Tengcha powder, add 12 - 20 times the volume of 60% ethanol, continuously reflux and extract at 50 - 70 °C in a water bath for 150 - 220 min. Keep the extraction process away from light and stir. Separate the solid and liquid to obtain the first extraction solution and the extraction residue. Conduct secondary continuous reflux extraction on the extraction residue under the same extraction conditions, separate the solid and liquid to obtain the secondary extraction solution. Combine the first and secondary extraction solutions, concentrate, and freeze-dry to obtain the freeze-dried powder of Tengcha extract;

[0019] 2) Weigh 8 - 12 parts of Astragalus membranaceus, add water with a volume 6 - 10 times that of Astragalus membranaceus, let it stand and soak for 20 - 50 min, then decoct in a water bath at 80 - 120 °C for 100 - 140 min. During decoction, assist with ultrasonic treatment, perform solid-liquid separation to obtain the first extraction solution and extraction residue. Under the same water extraction conditions, perform secondary ultrasonic extraction on the extraction residue, perform solid-liquid separation to obtain the secondary extraction solution, combine the first and secondary extraction solutions, concentrate, and freeze-dry to obtain the freeze-dried powder of Astragalus membranaceus extract;

[0020] 3) Weigh 8 - 12 parts of Pueraria lobata powder, add 50% ethanol with a volume 7 - 12 times that of Pueraria lobata powder, reflux and extract in a heating sleeve at 60 - 75 °C for 20 - 35 min, perform solid-liquid separation to obtain the first extraction solution and extraction residue. Under the same water extraction conditions, perform secondary extraction on the extraction residue, perform solid-liquid separation to obtain the secondary extraction solution, combine the first and secondary extraction solutions, concentrate, and freeze-dry to obtain the freeze-dried powder of Pueraria lobata extract;

[0021] 4) Weigh 8 - 10 parts of Crataegus pinnatifida powder, add water with a volume 15 - 20 times that of Crataegus pinnatifida powder, keep it warm and extract in a water bath at 70 - 90 °C for 70 - 100 min, perform solid-liquid separation to obtain the first extraction solution and extraction residue. Under the same extraction conditions, perform secondary extraction on the extraction residue, perform solid-liquid separation to obtain the secondary extraction solution, combine the first and secondary extraction solutions, concentrate, and freeze-dry to obtain the freeze-dried powder of Crataegus pinnatifida extract;

[0022] 5) Weigh 8 - 10 parts of Cassia obtusifolia powder, add water with a volume 7 - 15 times that of Cassia obtusifolia powder, keep it warm and extract in a water bath at 80 - 110 °C for 80 - 130 min, perform solid-liquid separation to obtain the first extraction solution and extraction residue. Under the same extraction conditions, perform secondary extraction on the extraction residue, perform solid-liquid separation to obtain the secondary extraction solution, combine the first and secondary extraction solutions, concentrate, and freeze-dry to obtain the freeze-dried powder of Cassia obtusifolia extract;

[0023] 6) Weigh the freeze-dried powders of different raw material extracts prepared in steps 1) - 5) according to the specified weight parts, and mix them to obtain the final product.

[0024] Furthermore, when concentrating the combined first and secondary extraction solutions in steps 1) - 5), they are all concentrated to 1 / 5 - 1 / 10 of the original volume.

[0025] Furthermore, the ultrasonic treatment power in step 2) is 450 - 500 W.

[0026] The above-mentioned food and medicine dual-purpose alcohol detoxification and liver protection composition can be used to prepare preparations for alleviating alcoholic liver damage and improving the liver's detoxification function. The preparations for alleviating alcoholic liver damage mainly inhibit inflammatory responses, reduce oxidative stress and promote stem cell regeneration by reducing liver cell damage and promoting repair; the preparations for improving the liver's detoxification function are mainly used to accelerate alcohol metabolism and reduce the accumulation of toxic intermediates, specifically involving: activating the activity of alcohol dehydrogenase (ADH) and acetaldehyde dehydrogenase (ALDH), shortening the retention time of acetaldehyde, increasing glutathione levels, neutralizing free radical toxicity, etc.

[0027] Furthermore, the dosage form of the preparation is selected from any one of powder, tablet, paste, capsule, granule, syrup, pill or oral film.

[0028] Furthermore, the alcoholic liver injury includes chronic alcoholic liver injury and acute alcoholic liver injury.

[0029] The food and medicine alcohol-detoxifying and liver-protecting composition can also be used as an active ingredient to prepare alcohol-detoxifying and liver-protecting beverages.

[0030] The beneficial effects of the present invention are:

[0031] 1. The edible and medicinal alcohol-detoxifying and liver-protecting composition disclosed in the present application is composed of a scientifically proportioned synergistic system of rattan tea, astragalus, kudzu root, hawthorn, and cassia seed. Among them, the flavonoids such as dihydromyricetin rich in rattan tea play an antioxidant and liver cell membrane stabilizing role by scavenging free radicals; puerarin targets and activates the alcohol dehydrogenase system, accelerates ethanol metabolism and reduces blood alcohol concentration; astragalus polysaccharide enhances the liver's glutathione synthesis ability based on its qi-invigorating and spleen-strengthening effects, and systematically improves the detoxification function; hawthorn organic acid and cassia seed chrysophanol are combined to synergistically promote the activation of lipid metabolism pathways, and achieve intervention through gastrointestinal motility enhancement and bile secretion regulation;

[0032] 2. Based on the synergistic mechanism of the formula ingredients, the dual activation pathways of alcohol dehydrogenase (ADH) gene expression and enzyme activity are targeted and regulated to eliminate the inhibitory effect of alcohol on ADH mRNA transcription and protein synthesis, significantly increase the ethanol metabolism rate to reduce the risk of liver damage; simultaneously enhance the activity level of acetaldehyde dehydrogenase (ALDH), effectively remove acetaldehyde toxic intermediates, block its pathological accumulation in the liver, and achieve the organic synergy of alcohol sobering and liver protection effects;

[0033] 3. The food and drug dual-purpose hangover and liver protection composition disclosed in this application takes natural components as the core, has both non-hepatotoxic characteristics and excellent biosafety, and is suitable for long-term intervention in alcohol-exposed populations. Its core mechanism of action is as follows: by inhibiting the abnormal increase of hepatocyte injury markers (alanine aminotransferase / aspartate aminotransferase), it alleviates the pathological process of alcoholic liver injury. At the same time, it targets and regulates lipid metabolism disorders (such as inhibiting the accumulation of triglycerides in the liver and intervening in the imbalance of high-density lipoprotein / low-density lipoprotein metabolism), and has the effect of reducing lipid deposition in the liver.

[0034] 4. The food and drug dual-purpose hangover and liver protection composition disclosed in this application achieves beneficial effects through the following synergistic mechanisms: significantly improving the α-diversity of the intestinal flora and reconstructing the β-diversity, restoring the balance of the intestinal microecology, and optimizing the flora structure; through the dual regulation of promoting mucus production in the positive direction and inhibiting excessive degradation in the reverse direction, enhancing the integrity of the intestinal physical barrier function, and effectively blocking the enterohepatic circulation of hepatotoxic substances such as acetaldehyde, the metabolite of ethanol; at the same time, promoting the specific enrichment of bacterial genera with liver-protective metabolic functions to achieve multi-dimensional liver protection effects.

[0035] 5. The food and drug dual-purpose hangover and liver protection composition disclosed in this application can be prepared into various dosage forms such as powders, tablets, capsules, granules, syrups, pills, or oral films, etc., which are suitable for the adjuvant treatment of alcoholic liver injury and the rapid relief of discomfort symptoms after drinking. The dual-mode intervention strategy of taking prophylactically before drinking or taking remedially after drinking can be adopted to achieve the effect of hangover and liver protection. It has the advantages of diverse dosage form selection, simple preparation process, high safety, etc., and has the feasibility of industrial implementation. Description of the Drawings

[0036] Figure 1 It is the in vitro antioxidant experiment results of plant extracts and formula compositions. Among them, sub-figure a is the DPPH free radical scavenging experiment result, sub-figure b is the ABTS free radical scavenging experiment result, and sub-figure c is the hydroxyl free radical scavenging experiment result.

[0037] Figure 2 It is a mouse alcoholic liver injury model induced by Lieber DeCarli alcohol diet. Among them, sub-figure a is the schematic diagram of model construction, sub-figure b is the liver index of each group of mice on the 17th day of administration, and sub-figure c is the liver photos of each group of mice on the 17th day of administration.

[0038] Figure 3 It is the level expression of relevant indicators in the serum and liver tissues of different groups of mice. Among them, sub-figure a is the alanine aminotransferase level in the serum, sub-figure b is the low-density lipoprotein level in the serum, sub-figure c is the triglyceride level in the serum, sub-figure d is the aspartate aminotransferase level in the serum, sub-figure e is the high-density lipoprotein level in the serum, and sub-figure f is the triglyceride level in the liver tissue.

[0039] Figure 4 H&E and Oil Red O staining images of liver tissue sections from different groups of mice;

[0040] Figure 5 Expression of related genes in the liver of different groups of mice. Among them, subfigure a shows the expression of alcohol dehydrogenase (ADH), subfigure b shows the expression of aldehyde dehydrogenase (ALDH), and subfigure c shows the expression of cytochrome P450 family 2E1 (CYP2E1);

[0041] Figure 6 Expression of oxidative stress level indicators in the liver of different groups of mice. Among them, subfigure a shows the enzyme activity level of superoxide dismutase, subfigure b shows the enzyme activity level of reduced glutathione, and subfigure c shows the malondialdehyde level of oxidative stress;

[0042] Figure 7 Effects of different treatments on the α-diversity and β-diversity of the intestinal flora in Lieber DeCarli alcohol-fed mice. Among them, subfigure a shows the expression of the Chao1 index of the mouse intestinal flora, subfigure b shows the expression of the Simpson index of the mouse intestinal flora, subfigure c shows the expression of the Shannon-Wiener index of the mouse intestinal flora. Subfigures a-c together reflect the changes in the α-diversity of the mouse intestinal flora; subfigure d shows the change in the Bray-Curtis distance of the mouse intestinal flora, subfigure e shows the change in the Unweighted UniFrac distance of the mouse intestinal flora, subfigure f shows the change in the Weighted UniFrac distance of the mouse intestinal flora. Subfigures d-f together reflect the changes in the β-diversity of the mouse intestinal flora;

[0043] Figure 8 Effects of different treatments on the change in β-diversity of the intestinal flora in Lieber DeCarli alcohol-fed mice. Among them, subfigure a is the non-metric multidimensional scaling analysis diagram of β-diversity, and subfigure b is the principal coordinate analysis diagram of β-diversity;

[0044] Figure 9 Effects of different treatments on the intestinal flora in Lieber DeCarli alcohol-fed mice. Among them, subfigure a shows the changes in bacterial genera at the phylum level, subfigure b shows the changes in bacterial genera at the genus level, and subfigure c is the phylogenetic tree diagram of the intestinal flora;

[0045] Figure 10 Linear discriminant analysis effect size diagram of the effects of different treatments on the intestinal flora in Lieber DeCarli alcohol-fed mice;

[0046] Figure 11Effects of different treatments on the abundances of beneficial functional bacteria in the intestinal flora of Lieber DeCarli alcohol-fed mice, where subfigure a is the content of the genus Bifidobacterium, subfigure b is the content of the genus Roseburia, and subfigure c is the content of the genus Eubacterium. Detailed implementation mode

[0047] To enable those of ordinary skill in the art to better understand the technical solution of the present invention, the technical solution of the present invention will be further described below with reference to the accompanying drawings and embodiments.

[0048] Example 1

[0049] This example discloses a food and drug dual-purpose hangover and liver protection composition, and its specific preparation method is as follows:

[0050] 1) Preparation of freeze-dried powder of Ampelopsis grossedentata extract: After crushing Ampelopsis grossedentata, weigh 3 portions of Ampelopsis grossedentata powder into a beaker, add 15 times the volume of 60% ethanol, continuously reflux and extract at 60 °C in a water bath for 180 min. Seal the container with plastic wrap and aluminum foil to reduce light exposure. During the extraction process, stir (150 rpm) to improve the extraction efficiency. Separate the solid and liquid to obtain aqueous extract a and aqueous extraction residue b. Add 15 times the volume of 60% ethanol to aqueous extraction residue b, continuously reflux and extract at 60 °C in a water bath for 180 min, separate the solid and liquid to obtain aqueous extract c. Combine aqueous extract a and aqueous extract c, concentrate to 1 / 5 of the original volume, and freeze-dry under reduced pressure to obtain the freeze-dried powder of Ampelopsis grossedentata extract.

[0051] 2) Preparation of freeze-dried powder of Astragalus membranaceus extract: After crushing Astragalus membranaceus, weigh 10 portions of Astragalus membranaceus into a beaker, add 8 times the volume of water, soak for 30 min, then decoct in a 100 °C water bath for 120 min. During the decoction, assist with 500 W ultrasonic treatment to promote the extraction of active ingredients in Astragalus membranaceus. Separate the solid and liquid to obtain aqueous extract a and aqueous extraction residue b. Add 8 times the volume of water to aqueous extraction residue b, decoct in a 100 °C water bath for 120 min, separate the solid and liquid to obtain aqueous extract c. Combine aqueous extract a and aqueous extract c, concentrate to 1 / 5 of the original volume, and freeze-dry under reduced pressure to obtain the freeze-dried powder of Astragalus membranaceus extract.

[0052] 3) Preparation of freeze-dried powder of Pueraria lobata extract: Weigh 10 portions of Pueraria lobata powder into a beaker, add 11 times the volume of 50% ethanol, reflux and extract in a 65 °C heating sleeve for 25 min. Separate the solid and liquid to obtain aqueous extract a and aqueous extraction residue b. Add 11 times the volume of 50% ethanol to aqueous extraction residue b, continue to reflux and extract in a 65 °C heating sleeve for 25 min, separate the solid and liquid to obtain aqueous extract c. Combine aqueous extract a and aqueous extract c, concentrate to 1 / 5 of the original volume, and freeze-dry under reduced pressure to obtain the freeze-dried powder of Pueraria lobata extract.

[0053] 4) Preparation of freeze-dried hawthorn extract: Weigh 10 parts of hawthorn powder into a beaker, add 20 times the volume of water, keep it warm and extract for 80 min under the water bath condition of 80 °C, separate the solid from the liquid to obtain aqueous extract a and water extraction filter residue b. Add 20 times the volume of water to the water extraction filter residue b, keep it warm and extract for 80 min under the water bath condition of 80 °C, separate the solid from the liquid to obtain aqueous extract c. Combine aqueous extract a and aqueous extract c, concentrate to 1 / 5 of the original volume, and freeze-dry under reduced pressure to obtain freeze-dried hawthorn extract.

[0054] 5) Preparation of freeze-dried cassia seed extract: Weigh 10 parts of cassia seed powder into a beaker, add 8 times the volume of water, keep it warm and extract for 120 min under the water bath condition of 100 °C, separate the solid from the liquid to obtain aqueous extract a and water extraction filter residue b. Add 8 times the volume of water to the water extraction filter residue b, keep it warm and extract for 120 min under the water bath condition of 100 °C, separate the solid from the liquid to obtain aqueous extract c. Combine aqueous extract a and aqueous extract c, concentrate to 1 / 5 of the original volume, and freeze-dry under reduced pressure to obtain freeze-dried cassia seed extract.

[0055] 6) By weight, weigh 4 parts of freeze-dried ampelopsis grossedentata extract, 5 parts of freeze-dried astragalus extract, 5 parts of freeze-dried kudzu root extract, 8 parts of freeze-dried hawthorn extract, and 2 parts of freeze-dried cassia seed extract prepared in steps 1)-5), and the formula composition can be obtained, which is the final product of the food and drug dual-purpose anti-alcoholism and liver-protecting composition to be prepared in this example.

[0056] Related performance tests:

[0057] 1. In vitro antioxidant experiments of plant extracts and formula compositions

[0058] 1) DPPH free radical scavenging experiment: Prepare a 0.15 mmol DPPH (1,1-diphenyl-2-trinitrophenylhydrazine) solution (dissolved in absolute ethanol) as the working solution; weigh freeze-dried kudzu root extract, freeze-dried hawthorn extract, freeze-dried ampelopsis grossedentata extract, and the formula composition, dissolve them with distilled water, and respectively prepare corresponding solutions with concentrations of 0.25 mg / mL, 0.5 mg / mL, 1 mg / mL, 2 mg / mL, 4 mg / mL, and 8 mg / mL as sample solutions; first take 1 mL of DPPH working solution, then take 1 mL of sample solution for mixing, vortex the mixed solution, and under the condition of avoiding light at 37 °C, after standing and reacting for 30 min, quickly measure the absorbance of the mixed solution at a wavelength of 517 nm. The calculation formula for the DPPH free radical scavenging rate is as follows:

[0059] DPPH free radical scavenging rate (%) = (A0 - A i + A j ) ÷ A0 * 100%

[0060] Among them, A i:Absorbance of 1 mL of DPPH working solution + 1 mL of sample solution;

[0061] A0: Absorbance of 1 mL of DPPH working solution + 1 mL of absolute ethanol;

[0062] A j : Absorbance of 1 mL of absolute ethanol + 1 mL of sample solution;

[0063] 2) ABTS radical scavenging experiment: Weigh 0.0192 g of ABTS (2,2'-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid), dissolve it in water to a volume of 5 mL to obtain solution A; weigh 7.6 mg of K2S2O4, add 200 μL of water to obtain solution B; add 89 μL of solution B to 5 mL of solution A, react in the dark for 12 h to prepare the ABTS mother solution; dilute the ABTS mother solution until the absorbance is near 0.7 to obtain the ABTS working solution.

[0064] Weigh the freeze-dried powder of Pueraria lobata extract, Hawthorn extract, Ampelopsis grossedentata extract and the formulated composition, dissolve them in distilled water, and prepare corresponding solutions with concentrations of 0.25 mg / mL, 0.5 mg / mL, 1 mg / mL, 2 mg / mL, 4 mg / mL, 8 mg / mL as sample solutions. First, take 1 mL of ABTS working solution, then take 1 mL of sample solution and mix them. Vortex the mixed solution, and under the condition of avoiding light at 37 °C, after standing and reacting for 1 h, quickly measure the absorbance of the mixed solution at a wavelength of 734 nm. The calculation formula for the ABTS radical scavenging rate is as follows:

[0065] ABTS radical scavenging rate (%) = (A0 - A i + A j ) ÷ A0 * 100%

[0066] Where, A i : Absorbance of 1 mL of ABTS working solution + 1 mL of sample solution;

[0067] A0: Absorbance of 1 mL of ABTS working solution + 1 mL of distilled water;

[0068] A j : Absorbance of 1 mL of distilled water + 1 mL of sample solution;

[0069] 3) Hydroxyl radical scavenging experiment: Prepare solutions of FeSO4·7H2O, H2O2 and salicylic acid with a concentration of 9 mmol / L respectively;

[0070] Weigh the freeze-dried powder of Pueraria lobata extract, freeze-dried powder of Crataegus pinnatifida extract, freeze-dried powder of Ampelopsis grossedentata extract and the formula composition, dissolve them with distilled water, and respectively prepare corresponding solutions with concentrations of 0.25 mg / mL, 0.5 mg / mL, 1 mg / mL, 2 mg / mL, 4 mg / mL, and 8 mg / mL as sample solutions. Add 1 mL of FeSO4·7H2O, salicylic acid, and H2O2 solutions to 1 mL of the sample solution, place it in a water bath at 37 °C for 30 min, then let it stand for 30 min, and measure the absorbance value at 510 nm; the clearance rate calculation formula is as follows:

[0071] The calculation formula for the hydroxyl radical scavenging rate is:

[0072] Scavenging rate (%) = (A0 - A i + A j ) ÷ A0 * 100%

[0073] A i : Absorbance of 3 mL of working solution + 1 mL of sample solution;

[0074] A0: Absorbance of 3 mL of working solution + 1 mL of distilled water;

[0075] A j : Absorbance of 3 mL of distilled water + 1 mL of sample solution;

[0076] For the results of the in vitro antioxidant experiment, see Figure 1 , and the IC50 values of the corresponding experiments are shown in Table 1.

[0077] Table 1. IC50 values of the in vitro antioxidant experiment of the freeze-dried powder of plant extracts and the formula composition

[0078] IC 50 Vitis pentagona (mg) Pueraria lobata (Willd.) Ohwi (mg) Fructus Crataegi (mg) Formulation composition (mg) DPPH free radical scavenging experiment 0.22 0.66 0.83 0.47 ABTS free radical scavenging experiment 0.03 0.42 0.66 0.06 Hydroxyl free radical scavenging experiment 0.01 0.44 0.26 0.09

[0079] During the alcohol metabolism process, a large amount of free radicals will be produced, leading to oxidative damage to the liver. Combining Figure 1 with the data in Table 1, it can be known that Ampelopsis grossedentata, Pueraria lobata, and Crataegus pinnatifida in the formula composition all contain a large amount of antioxidant components. These antioxidant components can neutralize these free radicals, reduce the oxidative stress on liver cells, thereby assisting in protecting liver function and promoting the repair of damaged liver cells.

[0080] 2. Construction of a mouse alcoholic liver injury model induced by Lieber-DeCarli alcohol diet

[0081] Male C57BL / 6J mice (purchased from Jicuiyaokang), 6 weeks old (20 ± 1 g), were randomly divided into 5 groups according to body weight, with 5 mice in each group, namely the blank control group, the model group, and the dosing groups (positive control group, low-concentration group, high-concentration group). Initially, all groups of mice were freely fed with control feed for 3 days to adapt to the liquid feed. Then, except for the control group (the control group needed to be fed with control feed at the same time), the remaining groups were fed with Lieber-DeCarli alcohol feed every day. First, they were adaptively fed with alcohol feed with a certain incremental amount for 7 days, and then fed with Lieber-DeCarli alcohol liquid feed containing 5% (v / v) ethanol for 17 days. While feeding the alcohol feed, the positive control group was intragastrically administered silymarin (SM, 10 μM) at 30 mg / kg body weight, and the low- and high-concentration groups were intragastrically administered the formulated composition at 10 mg / kg body weight and 30 mg / kg body weight respectively every day. The drugs were all dissolved in sterile water. The control group and the model group were intragastrically administered an equal amount of sterile water. The changes in body weight and food intake of each group of mice were recorded every day. On the 17th day of feeding Lieber-DeCarli alcohol liquid feed containing 5% (v / v) ethanol, each group of mice was intragastrically administered a single dose of ethanol (high dose, 5 g / kg body weight) at around 7 am, and euthanized 9 h later. The livers and whole blood of the mice were collected for subsequent experiments.

[0082] 3. Effects of the formulated composition on liver index and liver morphology of mice induced by Lieber DeCarli alcohol feed

[0083] Figure 2 Subfigure b shows the liver index of mice in each group on the 17th day of dosing (mouse liver mass / mouse body weight * 100%). An increase in the liver index usually represents an increase in liver weight and the occurrence of hepatocyte edema. Compared with the blank control group, the liver index of mice in the model group was significantly increased, indicating that the mouse model of alcoholic liver injury was successfully constructed. The liver index of mice in the dosing groups decreased compared with the model group, and the liver index of mice in the high-concentration group decreased most significantly.

[0084] From the liver morphology of each group of mice ( Figure 2 subfigure c), compared with the livers of mice in the blank control group raised in pairs, the livers of mice in the model group fed with alcohol showed obvious whitening and hypertrophy, while the low- and high-concentration groups could significantly improve this phenomenon.

[0085] 4. Effects of the formulated composition on the levels of ALT (alanine aminotransferase), AST (aspartate aminotransferase), HDL (high-density lipoprotein), LDL (low-density lipoprotein) in the serum of mice induced by Lieber-DeCarli alcohol feed and the levels of TG (triglyceride) in the serum and liver

[0086] Collect the whole blood of mice. After standing at room temperature for a period of time, centrifuge at 4°C and 4000 r·min -1 for 10 min, and collect the supernatant as serum. Use relevant kits to detect the levels of ALT, AST, HDL, and LDL in the serum of mice in each group, as well as the TG content in the serum and liver tissues of mice; the results are as Figure 3 shown. Compared with the blank control group, the levels of ALT, AST, and LDL in the serum of mice in the model group were significantly increased, while the HDL level was significantly decreased. Moreover, the TG content in the serum and liver tissues of mice in the model group was also significantly increased. However, compared with the model group, both the low- and high-concentration groups could significantly reduce the levels of ALT and AST in the serum of alcohol-fed mice ( Figure 3 subfigures a and d), relieve liver injury, and the high-concentration group had a better effect. In addition, compared with the model group, both the low- and high-concentration groups could significantly reduce the TG levels in the serum and liver tissues of alcohol-fed mice, as well as the LDL level in the serum and restore the HDL level. That is, this formula composition has the effect of reducing lipid deposition in the liver, and the high-concentration group has a better effect.

[0087] 5. Effects of the formula composition on the pathological staining results of the liver of mice induced by Lieber-DeCarli alcohol diet

[0088] The liver tissues fixed with paraformaldehyde were submitted to Sevier Biotechnology Co., Ltd. for cryo-embedding treatment, and HE and Oil Red O staining were performed. They were scanned using a digital pathology slide scanner (NanoZoomer S60) and analyzed using NDP software. The results are shown in Figure 4 . The results of HE staining showed that compared with the model group, the disorder of liver hepatocytes, fat vacuoles, and the expansion of cell gaps in the mice of the administration group were significantly improved, and the normal tissue structure of the liver was restored, and the high-dose group had a better effect. The results of Oil Red O staining showed that the lipid accumulation in the livers of mice in the low- and high-dose groups was also significantly improved, and the high-dose group had a more obvious effect.

[0089] 6. Effects of the formula composition on alcohol metabolism-related genes in the liver of mice induced by Lieber-DeCarli alcohol diet

[0090] Use an RNA extraction kit to extract the total RNA in the cells. After removing impurities, measure the RNA concentration with NanoDrop. Take 1 μg of RNA and reverse transcribe it into cDNA using a Vazyme kit. Dilute an appropriate amount of cDNA 5-fold with RNase Free dd H2O, prepare the PCR reaction solution according to the instructions, and the reaction conditions are shown in Table 2.

[0091] Table 2. RT-qPCR reaction conditions

[0092] Operation steps Temperature (°C) Time (s) Number of cycles Pre-denaturation 95 30 1 Denaturation 95 5 40 Annealing 60 10 40 Extension 72 15 40

[0093] The gene sequences of the relevant primers used in the experiment are shown in Table 3.

[0094] Table 3. Primer sequences of relevant genes

[0095]

[0096]

[0097] The experimental results showed that the expression levels of ADH and ALDH genes in the livers of mice in the model group were significantly decreased compared with those in the blank control group. However, compared with the model group, the expression levels of ADH and ALDH genes in the livers of mice in the low- and high-dose groups were significantly increased, and the improvement effect in the high-dose group was better. CYP2E1 is an alcohol oxidizing dehydrogenase, and it has been confirmed that it can be induced by alcohol in long-term alcoholics (the enzyme expression level and / or enzyme activity increase). When CYP2E1 metabolizes alcohol, it will produce a large amount of reactive oxygen species (ROS), which will aggravate the oxidative stress damage of hepatocytes and thus affect the process of ALD (alcoholic liver disease). Compared with the blank control group, the expression level of CYP2E1 gene in the liver of mice in the model group was significantly increased, but obvious inhibitory phenomena could be observed after administration, and the inhibitory effect in the high-dose group was more significant, indicating that the formulated composition can effectively increase the expression levels of alcohol metabolism-related enzymes and weaken the oxidative stress damage of hepatocytes, thereby increasing the alcohol metabolism level in the body ( Figure 5 ).

[0098] 7. Effect of the formulated composition on the oxidative stress level in the liver of Lieber-DeCarli alcohol-fed mice

[0099] An appropriate amount of mouse liver tissue was taken, homogenized by a magnetic bead tissue homogenizer, and then a commercial kit was used to detect the enzyme activities of GSH-Px (glutathione peroxidase) and SOD (superoxide dismutase) and the content of the oxidative stress index MDA (malondialdehyde) in the liver. The experimental results showed that under the oxidative stress state induced by alcohol, the content of MDA in the liver of mice in the model group was significantly increased compared with that in the blank control group, indicating an increase in lipid peroxidation. After administration intervention, the MDA level in the high-dose group was decreased compared with that in the model group, showing a dose-dependent improvement. At the same time, alcohol exposure significantly inhibited the activities of GSH-Px and SOD in the liver, but the activities of GSH-Px in the livers of mice in the low- and high-dose groups were significantly restored and exceeded those in the blank control group, and the activity of SOD recovered significantly, and the effect in the high-dose group was significantly better than that in the low-dose group. The above data indicate that the formulated composition can effectively alleviate the oxidative stress response in alcoholic liver injury through multi-pathway coordinated regulation ( Figure 6 ).

[0100] 8. Effects of the Formulation Composition on the Intestinal Flora of Mice Induced by Lieber-DeCarli Alcohol Diet

[0101] Mouse feces were collected in a sterile environment and submitted to the Bioinformatics Cloud Platform of Zhongke New Life for sample processing and analysis. The V3-V4 variable region was amplified and sequenced using the Illumina novaseq 6000 sequencing platform. To make the results of information analysis more accurate and reliable, the raw data was first subjected to quality filtering, noise reduction, splicing, and chimera removal using the default parameters of Qiime2, and sequences with an abundance of less than 10 (sum of all samples) were filtered out to obtain amplicon sequence variants. Based on the rarefied amplicon sequence variants, various diversity index analyses of the amplicon sequence variants and detection of sequencing depth can be performed. Based on taxonomic information, statistical analysis of the community structure can be carried out at various taxonomic levels.

[0102] 8.1. A significant increase in the α-diversity of the flora confirms the restoration of the intestinal microecology

[0103] Compared with the model group, the Chao1 index (composition), Simpson index (α-diversity), and Shannon-Wiener index (community evenness) of the intestinal flora of mice in the low- and high-concentration groups were all significantly increased statistically ( Figure 7 Subfigures a-c), suggesting that the formulation composition can dose-dependently restore the imbalance of the intestinal flora structure induced by alcohol. This technical feature confirms that the formulation composition can reconstruct the intestinal microecological environment damaged by alcohol exposure, enhance the stability and stress resistance of the flora system, and provide a basic guarantee for the metabolic activities of the subsequent functional flora.

[0104] 8.2. Reconstruction of β-diversity reveals optimization of the flora structure

[0105] Based on β-diversity analysis, the Bray-Curtis distance was significantly increased and the Weighted UniFrac distance was increased in the formulation intervention group compared with the alcohol model group, while only limited changes occurred in the Unweighted UniFrac distance, revealing that the composition specifically increased the relative abundances of core flora related to alcohol metabolism such as Bacteroidetes and Lachnospiraceae, and maintained the ecological niche of rare species such as Akkermansia, ultimately forming a flora functional network with high alcohol dehydrogenase (ADH) activity. ( Figure 7 Subfigures d-f, Figure 8 )

[0106] 8.3. Regulation at the phylum level reflects the mucosal barrier repair mechanism

[0107] Compared with the model group, the relative abundance of Actinobacteriota in the intestinal flora of mice in the high-concentration group increased ( Figure 9 subfigure a), and the core functional genus Bifidobacterium can enhance intestinal mucus secretion and inhibit pathogen colonization. At the same time, the abundance of Verrucomicrobiota decreased significantly ( Figure 9 subfigure a), especially the reduction of the mucus-degrading bacterium Akkermansia ( Figure 9 subfigure b) can effectively reduce the risk of excessive degradation of the intestinal mucus layer. This intestinal flora remodeling pattern protects the integrity of the intestinal physical barrier and effectively blocks the enterohepatic circulation of hepatotoxic substances such as acetaldehyde derived from ethanol metabolism through the synergistic effect of positively regulating mucus production (↑) and reversely inhibiting mucus decomposition (↓). ( Figure 9 、 Figure 10 )

[0108] 8.4, Specific enrichment of functional flora at the genus level promotes liver protection metabolism

[0109] The significant proliferation of the Lachnospiraceae NK4A136 group and the Clostridia UCG-014 group confirmed that the formulated composition can promote the colonization of short-chain fatty acid (SCFA)-producing bacteria. After the products such as butyric acid secreted by them are transported to the liver through the portal vein, they can activate the PPAR-γ pathway to inhibit liver inflammatory reactions; at the same time, precisely regulate the abundance callback of the mucus-degrading bacterium Akkermansia muciniphila, effectively reducing the increase in intestinal permeability induced by ethanol; in addition, the abundance of the genus Dubosiella in the intervention group decreased, inhibiting the liver oxidative stress caused by abnormal tryptophan metabolism.

[0110] From Figure 11 it can be seen that compared with the model group, the high-concentration group significantly increased the abundances of the three beneficial functional bacteria, Bifidobacterium, Roseburia, and Eubacterium, in the intestinal flora of mice, forming a multi-target protection mechanism: The Bifidobacterium genus competitively inhibits the proliferation of Gram-negative pathogenic bacteria and reduces the release of endotoxin (LPS); the butyrate produced by Roseburia can up-regulate the activities of liver ADH (alcohol dehydrogenase) and ALDH (aldehyde dehydrogenase) to promote ethanol metabolism; the secondary bile acid metabolism mediated by the Eubacterium genus can activate the farnesoid X receptor (FXR) in the liver, thereby regulating lipid metabolism homeostasis.

[0111] Example 2

[0112] The difference between this embodiment and Embodiment 1 lies in the different mixing ratios of the freeze-dried powders of different raw material extracts when preparing the formula composition. By weight, it includes 5 parts of freeze-dried powder of Ampelopsis grossedentata extract, 5 parts of freeze-dried powder of Astragalus membranaceus extract, 6 parts of freeze-dried powder of Pueraria lobata extract, 7 parts of freeze-dried powder of Crataegus pinnatifida extract, and 3 parts of freeze-dried powder of Cassia obtusifolia extract, thus obtaining the formula composition.

[0113] Verified, the formula composition disclosed in Embodiment 2 also has compound effects such as protecting the liver, relieving alcohol and dampness, antioxidative stress, and regulating lipid metabolism. And in terms of the effect, there is no significant difference compared with the composition disclosed in Embodiment 1.

[0114] Embodiment 3

[0115] The difference between this embodiment and Embodiment 1 lies in the different mixing ratios of the freeze-dried powders of different raw material extracts when preparing the formula composition. By weight, it includes 3 parts of freeze-dried powder of Ampelopsis grossedentata extract, 4 parts of freeze-dried powder of Astragalus membranaceus extract, 4 parts of freeze-dried powder of Pueraria lobata extract, 6 parts of freeze-dried powder of Crataegus pinnatifida extract, and 2 parts of freeze-dried powder of Cassia obtusifolia extract, thus obtaining the formula composition.

[0116] Verified, the formula composition disclosed in Embodiment 3 also has certain compound effects such as protecting the liver, relieving alcohol and dampness, antioxidative stress, and regulating lipid metabolism, but the effect is slightly lower than that of the composition disclosed in Embodiment 1.

[0117] The above shows and describes the basic principles, main features, and advantages of the present invention. However, the above are only specific embodiments of the present invention, and the technical features of the present invention are not limited thereto. Any other implementation manners obtained by those skilled in the art without departing from the technical solution of the present invention should be covered within the scope of the present invention.

Claims

1. A food and medicine composition for alcohol sobering up and protecting the liver, characterized in that: The raw materials of the composition include, by weight, 1 to 8 parts of freeze-dried powder of rattan tea extract, 1 to 8 parts of freeze-dried powder of astragalus extract, 1 to 5 parts of freeze-dried powder of kudzu root extract, 1 to 8 parts of freeze-dried powder of hawthorn extract and 1 to 5 parts of freeze-dried powder of cassia seed extract.

2. The food and medicine composition for alcohol sobering up and protecting the liver as claimed in claim 1, characterized in that: The raw materials of the composition include, by weight: 4 parts of freeze-dried powder of rattan tea extract, 5 parts of freeze-dried powder of astragalus extract, 5 parts of freeze-dried powder of kudzu root extract, 8 parts of freeze-dried powder of hawthorn extract and 2 parts of freeze-dried powder of cassia seed extract.

3. The method for preparing the edible and medicinal alcohol-relief and liver-protection composition according to any one of claims 1 to 2, characterized in that: The steps include: 1) Weigh 2 to 5 portions of rattan tea powder, add 12 to 20 times the volume of 60% ethanol, and continuously reflux and extract for 150 to 220 minutes in a water bath at 50 to 70° C., protect from light and stir during the extraction process, separate the solid and liquid, and obtain a primary extract and an extraction residue, perform a second continuous reflux extraction on the extraction residue under the same extraction conditions, separate the solid and liquid, and obtain a secondary extract, combine the primary and secondary extracts, concentrate, and freeze-dry to obtain a freeze-dried powder of rattan tea extract; 2) Weigh 8 to 12 portions of astragalus, add 6 to 10 times the volume of water, soak for 20 to 50 minutes, and then decoct in a water bath at 80 to 120° C. for 100 to 140 minutes, perform ultrasonic treatment during decoction, separate solid and liquid, and obtain a primary extract and an extraction residue, perform a secondary ultrasonic extraction on the extraction residue under the same extraction conditions, separate solid and liquid, and obtain a secondary extract, combine the primary and secondary extracts, concentrate, and freeze-dry to obtain a freeze-dried powder of astragalus extract; 3) Weigh 8 to 12 portions of kudzu root powder, add 7 to 12 times the volume of 50% ethanol, reflux extract at 60 to 75° C. for 20 to 35 minutes, separate the solid and liquid, obtain a primary extract and an extraction residue, perform a secondary extraction on the extraction residue under the same extraction conditions, separate the solid and liquid, obtain a secondary extract, combine the primary and secondary extracts, concentrate, and freeze-dry to obtain a freeze-dried powder of kudzu root extract; 4) Weigh 8 to 10 parts of hawthorn powder, add 15 to 20 times the volume of water, and extract under a water bath condition of 70 to 90° C. for 70 to 100 minutes, separate the solid and liquid, and obtain a primary extract and an extraction residue. Under the same extraction conditions, perform a secondary extraction on the extraction residue, separate the solid and liquid, and obtain a secondary extract. Combine the primary and secondary extracts, concentrate, and freeze-dry to obtain a freeze-dried hawthorn extract powder; 5) Weigh 8 to 10 portions of Cassia seed powder, add 7 to 15 times the volume of water, and extract at 80 to 110° C. in a water bath for 80 to 130 min, separate the solid and liquid, and obtain a primary extract and an extraction residue. Under the same water extraction conditions, perform a secondary extraction on the extraction residue, separate the solid and liquid, and obtain a secondary extract. Combine the primary and secondary extracts, concentrate, and freeze-dry to obtain freeze-dried powder of Cassia seed extract; 6) Weigh the freeze-dried powders of different raw material extracts prepared in steps 1) to 5) according to the specified weight proportions, and mix them thoroughly to obtain the final product.

4. The method for preparing the food and medicine dual-purpose alcohol sobering and liver protecting composition according to claim 3, characterized in that: In steps 1) to 5), the combined primary and secondary extracts are concentrated to 1 / 5 to 1 / 10 of their original volumes.

5. The method for preparing the food and medicine dual-purpose alcohol sobering and liver protecting composition according to claim 3, characterized in that: In step 2), the ultrasonic treatment power is 450-500W.

6. Use of the edible and medicinal alcohol-detoxifying and liver-protecting composition according to any one of claims 1 to 2 in the preparation of a preparation for alleviating alcoholic liver damage and enhancing liver detoxification function.

7. The use according to claim 6, characterized in that The dosage form of the preparation is selected from any one of powder, tablet, paste, capsule, granule, syrup, pill or oral film.

8. The use according to claim 6, characterized in that The alcoholic liver injury includes chronic alcoholic liver injury and acute alcoholic liver injury.

9. A drink for sobering up and protecting the liver, characterized in that: The edible and medicinal alcohol-resolving and liver-protecting composition according to any one of claims 1 to 2 is used as an active ingredient.

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