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Method for preparing dietary fiber from wheat bran

A technology for dietary fiber and wheat bran, which is applied in the directions of food preparation, application, food science, etc., can solve the problems of difficult control of product yield and quality, damage to the physiological activity of bran dietary fiber, pollution, etc.

Inactive Publication Date: 2012-12-05
BAOLINGBAO BIOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because general chemical reagents destroy the physiological activity of bran dietary fiber and cause serious pollution, it is not recommended to use chemical methods to prepare dietary fiber from bran. The traditional dietary fiber preparation process mostly uses acid and alkali solution alternate hydrolysis to remove Starch and protein, but the quality of the product after hydrolysis is poor, and the product yield and quality are not easy to control

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0020] Example 1: Add water to 1000g of wheat bran for pretreatment, the ratio of solid to liquid is 1:3, the temperature is 60°C, add amylase and glucoamylase, the addition amount is 10000IU, 130000IU respectively, keep stirring for 2h and then filter. The filter residue is refined to a particle size of 60 mesh. Add protease for hydrolysis, the amount of protease added is 15000IU, heat preservation and stirring for 2 hours, then filter, wash the filter residue with water once, mix the residue and water at a ratio of 1:2, and inactivate the enzyme at 110°C for 30 minutes. After deactivating the enzyme, the filter residue is diluted with water at a ratio of 1:8 to the bran pulp, and the cellulase is added for enzymolysis. The pH value is adjusted to 4.8. After 8 hours, continue to add xylanase for enzymatic hydrolysis, adjust the pH value to 7.0, enzymolysis temperature is 50°C, add enzyme in 3 times according to 300000IU, and enzymolysis time is 6h. The enzymatic solution was...

example 2

[0022] Example 2: Add water to 1500g of wheat bran for pretreatment, the ratio of solid to liquid is 1:4, the temperature is 62°C, add amylase and glucoamylase in the amount of 15000U and 195000IU respectively, keep stirring for 2 hours and then filter. The filter residue is refined to a particle size of 65 mesh. Add protease for hydrolysis, the amount of protease added is 7500IU, heat preservation and stirring for 2 hours, then filter, wash the filter residue with water once, mix the residue and water at a ratio of 1:2, and inactivate the enzyme at 110°C for 30 minutes. After deactivating the enzyme, the filter residue was diluted with water at a ratio of 1:9 to the bran pulp, and cellulase was added for enzymolysis. After 8 hours, continue to add xylanase for enzymatic hydrolysis, adjust the pH value to 7.3, enzymatic hydrolysis temperature at 50°C, add enzyme in 450000IU divided into 3 times, and enzymatic hydrolysis time 6h. The enzymatic solution was concentrated to 20% ...

example 3

[0024] Example 3: Add water to 2000g of wheat bran for pretreatment, the ratio of solid to liquid is 1:5, the temperature is 65°C, add amylase and glucoamylase in the amount of 20000U and 260000IU respectively, keep stirring for 2 hours and then filter. The filter residue is refined, and the particle size is 70 mesh. Add protease for hydrolysis, the amount of protease added is 30,000 IU, keep stirring for 2 hours, then filter, wash the filter residue with water once, mix the residue and water at a ratio of 1:2, and inactivate the enzyme at 110°C for 30 minutes. After deactivating the enzyme, the filter residue was diluted with water at a ratio of 1:10 to dilute the bran pulp, and then added cellulase for enzymolysis, the pH value was adjusted to 5.2, the enzymatic hydrolysis temperature was 50°C, and the amount of enzyme added was 600,000 IU in three separate additions, and the enzymatic hydrolysis time 8h, then continue to add xylanase for enzymolysis, the pH value is adjuste...

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Abstract

The invention relates to a method for preparing a dietary fiber from wheat bran, comprising the following steps: mixing the wheat bran with water for pretreatment, adding amylase and glucoamylase, preserving heat, stirring for 2 hours, filtering, removing slag by filtering, making a thick liquid by grinding, adding amylase for hydrolyzing, filtering, carrying out enzyme deactivation, adding cellulose and xylanase for synergistic enzymolysis to obtain the diluted solution of a crude water-soluble dietary fiber, concentrating, decolorizing and carrying out chromatographic separation to obtain the high-purity water-soluble dietary fiber. The dietary fiber prepared by adopting the method has the purity of more than 90% and can be used as the dietary fiber nutrition fortifier of various foods.

Description

technical field [0001] The present invention relates to a preparation method of dietary fiber, in particular to a preparation method of dietary fiber using wheat bran as raw material. Background technique [0002] Dietary fiber is a food nutrient that is generally difficult to digest. It refers to the sum of edible plant cells, polysaccharides, lignin and related substances that cannot be digested and absorbed by human endogenous enzymes. As the seventh largest nutrient, it is indispensable for a healthy diet. It not only has high nutritional value, but also prevents many diseases, and is essential for human metabolism. Dietary fiber can prevent constipation, lower serum cholesterol, regulate blood sugar levels, prevent gallstones, absorb sodium ions, lower blood pressure, lose weight and fight cancer. [0003] With the improvement of people's living standards and changes in lifestyles, people's daily diet structure has undergone tremendous changes, and "civilized diseases"...

Claims

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Application Information

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IPC IPC(8): A23L1/10A23L1/308A23L7/10
Inventor 刘宗利王乃强袁卫涛栾庆民
Owner BAOLINGBAO BIOLOGY
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