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Method for degrading enteromorpha prolifera polysaccharides by enzymic method

A technology for enzymatic degradation of E. prolifera polysaccharide, applied in the field of food biology, can solve the problems of difficulty and few reports of enzymatic degradation of E. prolifera polysaccharide, and achieves mild action conditions, good in vitro antioxidant activity, and short reaction time. Effect

Active Publication Date: 2014-07-30
浙江欧托电气有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Enzymatic degradation has mild conditions and can also avoid the loss of sulfate groups, but it is not easy to obtain enzymes with high hydrolysis efficiency
However, there are few reports on the enzymatic degradation of Enteromorpha polysaccharides

Method used

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  • Method for degrading enteromorpha prolifera polysaccharides by enzymic method
  • Method for degrading enteromorpha prolifera polysaccharides by enzymic method
  • Method for degrading enteromorpha prolifera polysaccharides by enzymic method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1-1

[0041] Embodiment 1-1, the method for enzymatic degradation of enteromorpha polysaccharide:

[0042] The crude polysaccharide of Enteromorpha enteromorpha with a purity of 75.45% is dissolved into a crude polysaccharide solution of 5 mg / mL with the acetic acid-sodium acetate buffer (the total concentration of acetic acid and sodium acetate is 0.1mol / L) with a pH of 4.5 .

[0043] 10×10 4 U / mL glucoamylase (glucoamylase) was diluted to 100 U / mL with pH 4.5 acetic acid-sodium acetate buffer (the total concentration of acetic acid and sodium acetate was 0.1 mol / L) to obtain a glucoamylase enzyme solution. Take 710 μL of glucoamylase enzyme solution and make up to 1 mL with distilled water to obtain the diluted glucoamylase enzyme solution.

[0044] Then add 1ml diluted glucoamylase enzyme solution to 4mL crude polysaccharide solution, so that the concentration of glucoamylase is 14.2U / mL, and the concentration of Enteromorpha crude polysaccharide is 4mg / mL; at this time, the pH...

Embodiment 1-2

[0050]Embodiment 1-2, enteromorpha polysaccharide is enzymatically hydrolyzed with glucoamylase:

[0051] The reaction time among the embodiment 1-1 is changed into 4 hours from 2 hours, all the other are the same as embodiment 1-1.

[0052] Enzymatically hydrolyzed Enteromorpha polysaccharides with a degradation rate of 11.6% were obtained.

[0053] Experiment 1,

[0054] Using literature (Espin J C, Soler-Rivas C, Wichers H J, et al. Anthocyanin-based natural colorants: a new source of antiradical activity for foodstuff [J]. Journal Agricultural Food Chemistry, 2000, 48 (5): 1588-1592. ) report method, the enzymolysis Enteromorpha polysaccharide obtained in Example 1-1 is carried out the test of DPPH free radical scavenging ability, and compares with undegraded polysaccharide. Depend on figure 1 It can be seen that after the enzymatic hydrolysis of Enteromorpha polysaccharides, the scavenging ability of DPPH free radicals has been significantly improved. When the concent...

Embodiment 2

[0067] Embodiment 2, the method for enzymatic degradation of enteromorpha polysaccharide

[0068] Enteromorpha crude polysaccharides with a purity of 75.45% were dissolved in acetic acid-sodium acetate buffer solution (0.1 mol / L) at pH 4.5 to form a crude polysaccharide solution with a concentration of 5 mg / mL.

[0069] 10×10 4 U / mL glucoamylase (glucoamylase) was diluted to 100 U / mL with pH 4.5 acetic acid-sodium acetate buffer (0.1 mol / L) to obtain a glucoamylase enzyme solution.

[0070] Dilute 156 mg of pectinase (enzyme activity: 30 U / mg) to 100 mL with pH 4.5 acetic acid-sodium acetate buffer (0.1 mol / L); obtain pectinase enzyme solution.

[0071] Then add 1ml diluted glucoamylase enzyme solution to 4mL crude polysaccharide solution, so that the enzyme concentration is 14.2U / mL, and the concentration of Enteromorpha crude polysaccharide is 4mg / mL; at this time, the pH is 4.5, and the reaction time is 49°C 2 hours.

[0072] Boil the polysaccharide solution after enzymo...

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Abstract

The invention discloses a method for degrading enteromorpha prolifera polysaccharides by an enzymic method. The method disclosed by the invention is characterized by comprising the following steps: preparing a polysaccharide solution with concentration of 1-20mg / mL from crude enteromorpha prolifera polysaccharides or enteromorpha prolifera polysaccharides; then with glucoamylase as hydrolase, reacting for 1-5 hours under the conditions that the pH value is 3.5-5.5 and the temperature is 40-60 DEG C, thus obtaining a solution of polysaccharides degraded through enzymolysis for the first time; boiling the solution of polysaccharides degraded through enzymolysis for the first time for 4-6 minutes and then centrifuging the solution of polysaccharides degraded through enzymolysis for the first time to remove glucoamylase, thus obtaining supernatant containing enteromorpha prolifera polysaccharides degraded through enzymolysis for the first time; and dialyzing and freeze-drying the supernatant, thus obtaining powdery enteromorpha prolifera polysaccharides (with molecular weight range of 7.2-122kDa) degraded through enzymolysis. The method has the characteristics of high hydrolysis efficiency and high in vitro antioxidative activities of obtained polysaccharides.

Description

technical field [0001] The invention belongs to the field of food biotechnology and relates to a method for enzymatically degrading polysaccharides of Enteromorpha enteromorpha. Background technique [0002] Enteromorpha is a large-scale economical green algae in the southeast coast of my country. It is rich in resources and can be eaten by itself. It also contains a variety of active substances, such as Enteromorpha polysaccharides, lipid pigments, and phenols. According to literature reports, Enteromorpha polysaccharide has physiological functions such as improving immunity of mammals, lowering blood fat, anti-inflammatory, antibacterial and anti-oxidation. Therefore, the development of Enteromorpha into functional food has a good prospect. The polysaccharide extracted from Enteromorpha is mainly water-soluble sulfated polysaccharide, and its composition is mainly glucuronic acid-xylose-rhamnose polymer. Enteromorpha polysaccharide has a large molecular weight. If it is ...

Claims

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Application Information

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IPC IPC(8): C08B37/00
Inventor 周涛许莉莉
Owner 浙江欧托电气有限公司
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