Nutritional compositions containing a neurologic component and uses thereof
A nutritional composition, a technology of nutrients, applied in the field of promoting brain and nervous system health
Image
Examples
Embodiment 1
[0189] This example describes the neurogenesis of hADSCs induced by PE compared to DHA and negative controls.
[0190] PE from bovine and plant were purchased from Matreya® (Cat. No. 1069) and (Cat. No. 1301 ), respectively. PE from bovine was diluted to 67.2 mM in 100% ethanol. Dilute plant-derived PE to 67.6 mM in 100% ethanol. These solutions were then stored at 4-8°C.
[0191] hADSCs were purchased from Invitrogen, Carlsbad, CA, USA, aka Life Technologies, in 100 mm culture plates in a maintenance medium consisting of Complete MesenPro RS medium with growth supplements from Invitrogen® and L-glutamine A near confluent monolayer was cultured. Methods for culturing, passaging and seeding hADSCs are described below.
[0192] Subculture of hADSCs was performed when the cell cultures reached confluence. To passage hADSCs, the following methods were used: i) aspirating the Complete MesenPRO RS Medium from the cells; ii) removing the surface of the cell layer by adding DPBS ...
Embodiment 2
[0207] This example describes the neurogenesis of hADSCs induced by sphingomyelin compared to DHA and negative controls.
[0208] Sphingomyelin from eggs (Cat. No. 1332) and skim milk (Cat. No. 1329) were purchased from Matreya (Pleasant Gap, PA, USA). Sphingomyelin from eggs and skim milk were each diluted to 13.7 mM in 100% ethanol. By the same method outlined in Example 1, hADSCs were cultured, passaged, seeded, and sphingomyelin treated.
[0209] hADSCs including 10 μM sphingomyelin, 20 μM sphingomyelin, 40 μM sphingomyelin, 10 μM DHA and a negative control were observed under a phase-contrast microscope at 24 hours, 48 hours and 96 hours after treatment.
[0210] Even at low concentrations of sphingomyelin, most extensions, although not very long, were longer than the negative control, and many more. Bovine sphingomyelin at 40 μM and skimmed milk sphingomyelin at 20 μM was more effective than DHA in this regimen. see Figure 3A , 3B and 3C.
[0211] Among the additi...
Embodiment 3
[0213] This example describes the neurogenesis of hADSCs induced by CDP-choline compared to DHA and negative controls.
[0214] CDP-choline was obtained from Kyowa Hakko Gio Co. In a laminar flow hood, dissolve CDP-choline in sterile H 2 O to 200 μM to obtain a clear stock solution. By the same method outlined in Example 1, hADSCs were cultured, passaged, plated, and treated with CDP-choline.
[0215] CDP-choline treatment was tested at 5 μM and 10 μM concentrations. Different concentrations of CDP-choline were tested and compared with the positive control, 10 μM DHA ( Figure 4A ) and negative control ( Figure 4B ). Experiments were repeated in triplicate.
[0216] Furthermore, CDP-choline at a concentration of 5 μM was shown to enhance neurogenesis due to the observed neurite outgrowth and neuronal morphological changes were observed in hADCS treated with CDP-choline. see Figure 4C . Note that the cytoplasm shrinks and the neurites begin to protrude. A halo (coro...
PUM
Login to View More Abstract
Description
Claims
Application Information
- IPC
- A23L1/30; A23L1/29; A61K31/164; A61K31/685; A61K31/688; A61K31/7032; A61K31/7068; A61K31/7072; A23L33/00
- CPC
- A61K31/7072; A23L1/3008; A61K31/07; A61K31/685; A61K31/7068; A61K31/164; A61K31/688; A23L1/296
- Inventors
- C.邝; Y.肖