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Nutritional compositions containing a neurologic component and uses thereof

A nutritional composition, a technology of nutrients, applied in the field of promoting brain and nervous system health

Inactive Publication Date: 2015-09-02
MJN U S HLDG LLC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

CDP-choline formation is the slowest step in the phospholipid metabolism pathway, thereby limiting the entire pathway

Method used

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  • Nutritional compositions containing a neurologic component and uses thereof
  • Nutritional compositions containing a neurologic component and uses thereof
  • Nutritional compositions containing a neurologic component and uses thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0189] This example describes the neurogenesis of hADSCs induced by PE compared to DHA and negative controls.

[0190] PE from bovine and plant were purchased from Matreya® (Cat. No. 1069) and (Cat. No. 1301 ), respectively. PE from bovine was diluted to 67.2 mM in 100% ethanol. Dilute plant-derived PE to 67.6 mM in 100% ethanol. These solutions were then stored at 4-8°C.

[0191] hADSCs were purchased from Invitrogen, Carlsbad, CA, USA, aka Life Technologies, in 100 mm culture plates in a maintenance medium consisting of Complete MesenPro RS medium with growth supplements from Invitrogen® and L-glutamine A near confluent monolayer was cultured. Methods for culturing, passaging and seeding hADSCs are described below.

[0192] Subculture of hADSCs was performed when the cell cultures reached confluence. To passage hADSCs, the following methods were used: i) aspirating the Complete MesenPRO RS Medium from the cells; ii) removing the surface of the cell layer by adding DPBS ...

Embodiment 2

[0207] This example describes the neurogenesis of hADSCs induced by sphingomyelin compared to DHA and negative controls.

[0208] Sphingomyelin from eggs (Cat. No. 1332) and skim milk (Cat. No. 1329) were purchased from Matreya (Pleasant Gap, PA, USA). Sphingomyelin from eggs and skim milk were each diluted to 13.7 mM in 100% ethanol. By the same method outlined in Example 1, hADSCs were cultured, passaged, seeded, and sphingomyelin treated.

[0209] hADSCs including 10 μM sphingomyelin, 20 μM sphingomyelin, 40 μM sphingomyelin, 10 μM DHA and a negative control were observed under a phase-contrast microscope at 24 hours, 48 ​​hours and 96 hours after treatment.

[0210] Even at low concentrations of sphingomyelin, most extensions, although not very long, were longer than the negative control, and many more. Bovine sphingomyelin at 40 μM and skimmed milk sphingomyelin at 20 μM was more effective than DHA in this regimen. see Figure 3A , 3B and 3C.

[0211] Among the additi...

Embodiment 3

[0213] This example describes the neurogenesis of hADSCs induced by CDP-choline compared to DHA and negative controls.

[0214] CDP-choline was obtained from Kyowa Hakko Gio Co. In a laminar flow hood, dissolve CDP-choline in sterile H 2 O to 200 μM to obtain a clear stock solution. By the same method outlined in Example 1, hADSCs were cultured, passaged, plated, and treated with CDP-choline.

[0215] CDP-choline treatment was tested at 5 μM and 10 μM concentrations. Different concentrations of CDP-choline were tested and compared with the positive control, 10 μM DHA ( Figure 4A ) and negative control ( Figure 4B ). Experiments were repeated in triplicate.

[0216] Furthermore, CDP-choline at a concentration of 5 μM was shown to enhance neurogenesis due to the observed neurite outgrowth and neuronal morphological changes were observed in hADCS treated with CDP-choline. see Figure 4C . Note that the cytoplasm shrinks and the neurites begin to protrude. A halo (coro...

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Abstract

The present disclosure relates to nutritional compositions comprising a neurologic component, wherein, the neurologic component may promote brain and nervous system development and further provide neurological protection and repair. The neurologic component may include phosphatidylethanolamine, sphingomyelin, cytidine diphosphate-choline, ceramide, uridine, at least one ganglioside, and mixtures thereof. The disclosure further relates to methods of promoting brain and nervous system health by providing said nutritional compositions to target subjects, which includes pediatric subjects.

Description

technical field [0001] The present disclosure relates to nutritional compositions comprising neural components suitable for administration to adult and pediatric subjects. Neural components may include phosphatidylethanolamine ("PE"), sphingomyelin, cytidine diphosphate-choline ("CDP-choline"), ceramide, uridine, at least one ganglioside, and two or more mixtures. The neural components provide additive and / or synergistic beneficial health benefits, including enhanced brain development and improved memory, cognition, hand-eye coordination, and improved concentration. [0002] Additionally, the present disclosure relates to methods of promoting brain and nervous system health by providing nutritional compositions comprising the neural components described herein. Background technique [0003] The brain accounts for only 2% of total body weight, but is a demanding organ that uses up to 30% of daily calories and nutrients (Harris, J.J. et al., The Energetics of CNS White Matt...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L1/30A23L1/29A61K31/164A61K31/685A61K31/688A61K31/7032A61K31/7068A61K31/7072A23L33/00
CPCA61K31/7072A23L1/3008A61K31/07A61K31/685A61K31/7068A61K31/164A61K31/688A23L1/296A23L1/3006A61K31/7032A23L33/40A23L33/115A23L33/12A61K2300/00
Inventor C.邝Y.肖E.K.佩尔斯Z.朱尼D.洪曼
Owner MJN U S HLDG LLC
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