Giant magneto-impedance effect biosensor for detecting serum tumor markers

Abstract

The invention provides a giant magneto-impedance effect biosensor for detecting serum tumor markers. The biosensor consists of a giant magneto-impedance effect sensor positioned on a glass substrate, an insulated layer, an Au film, a biologically sensitive film, a magnetic tag and a signal acquisition system, wherein the giant magneto-impedance effect sensor consists of the NiFe / Cu / NiFe multi-layer film on the glass substrate and is made by the microelectromechanical system (MEMS) technology; the insulated layer is the aluminum oxide layer, the Au film is the splashed Cr / Au film, and the biologically sensitive film is the self-assembled film with nano-level thickness and can be connected with various monoclonal antibodies; and the magnetic tag is streptavidin-modified magnetic nano particles or magnetic beads consisting of magnetic nano particles and can be combined with biotin-modified monoclonal antibodies or polyclonal antibodies. Based on the detection principle of a double-antibody sandwich method, the sensor has a high detection sensitivity and can detect the antigens of which the content of serum tumor markers is as high as 1ng / ml.

Description

technical field [0001] The invention relates to a biosensor, in particular to a giant magneto-impedance effect biosensor for detecting serum tumor markers, and belongs to the technical fields of medical detection methods and magnetic sensing. Background technique [0002] Malignant tumors are one of the major diseases that threaten human health and life today, and the incidence rate is increasing year by year. At present, the main clinical tumor detection methods include: [0003] [1] Laboratory examination: mainly includes routine examination of blood, urine, and stool, biochemical and immune examination, pathological examination, etc. [0004] [2] Radiological examination: mainly including X-ray fluoroscopy, X-ray film, X-ray contrast examination, CT scan, nuclear magnetic resonance imaging, etc. [0005] [3] Radionuclide inspection: that is, isotope inspection, including functional measurement inspection, scanning and gamma irradiation inspection, radioimmunoassay, etc....

AI Technical Summary

Problems solved by technology

However, the shortcomings of these methods are the lack of flexibility, low sensitivity and poor specificity of single indicators.

In 2007, Kurlyandskaya et al. (A.Kumar, S.Mohapatra, V.F.Miyar, A.Cerdeira, J.A.Garcia, H.Srikanth, J.Gass and G.V.Kurlyandskaya) published in "Appl.Phys.Lett." (American Applied Physics Letters) "Vol.91, pp.143902, 2007" reported the use of GMI sensors to detect human embryonic kidney cells (HEK293) that phagocytized Fe3O4 nanoparticles, proving the feasibility of the GMI effect in the field of biomedical detection, but this research work is only The response of the GMI sensor to Fe3O4 magnetic nanoparticles was obtained, but the labeling and typing detection of cell samples were not realized

Through literature and patent searches, no relevant research results were found on the use of GMI effect sensors for the detection of serum tumor markers

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