Exonuclease III digesting FRET-dsDNA microarray chip for detecting transcription factor protein

Abstract

Disclosed is a transcription factor protein detection method through exonuclease III digestion FRET-dsDNA micro array chips, wherein the expression and activation levels of the transcription factor are analyzed through the following steps: (1) preparing FRET-dsDNA micro array chips, (2) reacting the transcription factor with FRET-dsDNA micro array chips, (2) reacting the exonuclease III with FRET-dsDNA micro array chips, (1) proceeding detection and analysis to the FRET-dsDNA micro array chips.

Description

1. Technical field: [0001] Transcription factor (transcription factor) is a kind of important protein responsible for gene expression regulation in proteomics. ) and proteome research; many diseases are closely related to the abnormal expression and activation of transcription factors, and have become important targets for transcriptional therapy and drug research (drug target). The detection and analysis of transcription factor expression and activation degree is the main means to study its function. This patent proposes a new method for detecting the expression and activation level of transcription factors, which will be used in the research of transcription factors in the fields of molecular biology, functional genomics, proteomics and biomedicine Provide a new detection and analysis technology that can promote scientific research on transcription factors in these fields, and provide a diagnostic technology for disease-related transcription factors in the biomedical field,...

AI Technical Summary

Problems solved by technology

The main advantage of method 1 is that only two DNA half-sites labeled by the donor and the acceptor are needed to detect the protein, the experiment is simple and efficient, and the specificity is good; however, there are difficulties in the design of the DNA half-site and receptor tags inserted into the protein binding site may prevent the normal binding of the protein or even prevent it from binding, and for those proteins whose DNA binding site is not long, it is more difficult or even impossible to design DNA half-sites, even if Possibly, such DNA half-sites are difficult to bind proteins

Method 2 does not require the design of DNA half-sites, but requires the preparation of protein antibodies and fluorescently labeled antibodies, and the greatest difficulty lies in the design and preparation of antibodies that are spatially close enough to the donor (or acceptor) markers through protein binding and acceptor (or donor) labeled DNA is not easy, even difficult

Images