Important gene enrichment method used for individual cancer diagnosis and treatment

Abstract

The invention discloses an important gene enrichment method used for individual cancer diagnosis and treatment. A combination of common cancer genes of Chinese patients is established, an enrichment scheme is designed according to the mutation characteristics of the genes of Chinese patients to achieve efficient targeting enrichment of important cancer genes, and the sequencing flux of above 20000 genes reduces to below 200 genes, so the sequencing cost is substantially reduced. The method containing the enrichment scheme of gene transposition and other special mutations can detect the mutation which cannot be detected by an exon and is important for diagnosis and treatment.

Description

technical field [0001] The invention relates to a gene enrichment method. Background technique [0002] A large amount of evidence shows that cancer is a "gene" disease, and the accumulation of gene mutations on genomic DNA is the main reason why normal cells turn into cancer cells. It can be seen that thoroughly interpreting the DNA "Book" of cancer genomes and finding out the differences between cancer cells and normal cells may become an important basis for the specific diagnosis and treatment of cancer. [0003] With the completion of the Human Genome Project and the advancement of DNA sequencing technology, it is possible to systematically interpret the cancer genome. A large number of such studies have shown that the genetic mutations of seemingly identical cancers are highly heterogeneous. DNA sequencing is performed on individual patient tumors to identify unique genetic mutations, and it is possible to select targeted and specific treatment options. , improve the ...

AI Technical Summary

Problems solved by technology

This type of whole-exome sequencing covers all 20,000+ genes and is expensive