Important gene enrichment method used for individual cancer diagnosis and treatment

An important, genetic technology, applied in the field of gene enrichment, can solve problems such as high price

Inactive Publication Date: 2014-10-22
GENETRON HEALTH (BEIJING) CO LTD
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AI Technical Summary

Problems solved by technology

This type of whole-exome sequencing covers all 20,000+ genes and is expensive

Method used

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Embodiment Construction

[0014] The important gene enrichment method for individualized cancer diagnosis and treatment of the present invention comprises the following steps:

[0015] Prepare the corresponding DNA probe microarray, connect the DNA probe containing the target region into the transcription expression vector, and express the RNA probe in vitro;

[0016] Use biotin-labeled single bases to synthesize RNA, so that the synthesized RNA probes are fully biotin-labeled;

[0017] The DNA to be tested is broken into small fragments of 300-500bp through a non-contact ultrasonic crushing scheme, and the ends of the small fragments are filled in with a molecular biology scheme, and an "A" base is added and combined with a "T" base Sequencing ligand ligation;

[0018] Use the amplification primers corresponding to the ligand to amplify the ligation product to obtain a genome sequencing library;

[0019] The sequencing library was heated at 95 degrees Celsius for 5 minutes to form single-stranded DN...

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Abstract

The invention discloses an important gene enrichment method used for individual cancer diagnosis and treatment. A combination of common cancer genes of Chinese patients is established, an enrichment scheme is designed according to the mutation characteristics of the genes of Chinese patients to achieve efficient targeting enrichment of important cancer genes, and the sequencing flux of above 20000 genes reduces to below 200 genes, so the sequencing cost is substantially reduced. The method containing the enrichment scheme of gene transposition and other special mutations can detect the mutation which cannot be detected by an exon and is important for diagnosis and treatment.

Description

technical field [0001] The invention relates to a gene enrichment method. Background technique [0002] A large amount of evidence shows that cancer is a "gene" disease, and the accumulation of gene mutations on genomic DNA is the main reason why normal cells turn into cancer cells. It can be seen that thoroughly interpreting the DNA "Book" of cancer genomes and finding out the differences between cancer cells and normal cells may become an important basis for the specific diagnosis and treatment of cancer. [0003] With the completion of the Human Genome Project and the advancement of DNA sequencing technology, it is possible to systematically interpret the cancer genome. A large number of such studies have shown that the genetic mutations of seemingly identical cancers are highly heterogeneous. DNA sequencing is performed on individual patient tumors to identify unique genetic mutations, and it is possible to select targeted and specific treatment options. , improve the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
CPCC12N15/1013C12Q1/6806C12Q2537/159C12Q2563/131C12Q2535/122
Inventor 阎海焦宇辰王晓月王思振熊梓锴
Owner GENETRON HEALTH (BEIJING) CO LTD
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