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Method and apparatus for bio-molecular chip minute quantity sample application and reaction

A biomolecular and micro-sampling technology, applied in biochemical equipment and methods, measuring devices, chemical instruments and methods, etc., can solve the problems of complicated micro-channel fabrication, cumbersome preparation process, application limitations, etc., and shorten the reaction time. , the effect of accelerating mass transfer rate and improving quality

Inactive Publication Date: 2004-08-25
INST OF MECHANICS - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The micro-channel used in this method is disposable, and the micro-channel is complicated to make and the cost is high, so that the application is limited
Simultaneous biomolecule immobilization and detection reactions are performed twice in two devices, so the preparation process is cumbersome

Method used

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  • Method and apparatus for bio-molecular chip minute quantity sample application and reaction
  • Method and apparatus for bio-molecular chip minute quantity sample application and reaction
  • Method and apparatus for bio-molecular chip minute quantity sample application and reaction

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] Make the first device, and carry out the method of immobilization and detection of twelve kinds of proteins on it. The device includes twelve grooves. Groove area is 1mm 2 , the depth is 0.1mm, and the inner diameter of the microchannel is 0.5mm. The elastic material is rubber, the solid material is plexiglass, the tube is polytetrafluoroethylene, and the elastic material membrane for closure is silicone. The chip material is silicon.

[0053] Make a microarray template according to Figure 1-2; use rubber with a thickness of 1mm as the elastic material block 3, and twelve grooves 2 are arranged in an array on it, and the area of ​​the grooves 2 is 1mm 2 , with a depth of 0.1 mm; a first through hole 10 with an inner diameter of 0.5 mm is opened at both ends of the groove 2 as a micro-channel 5 (as shown in FIG. 1 ). The plexiglass with a thickness of 5mm is used as the solid material block 4, and twelve grooves 2' are arranged in an array on the plexiglass block 4, ...

Embodiment 2

[0063] A device of the invention was prepared having 100 individual sealed cavities.

[0064] The elastic material block 3 of this embodiment is silica gel, and includes 100 grooves 2 engraved on it. The area of ​​the groove 2 is 0.1mm 2 or 0.01mm 2 The depth is 0.1mm; the first through hole 10 with an inner diameter of 0.2mm is opened at both ends of the groove 2, and a piece of solid material 4 is aluminum, which includes 100 grooves 2' engraved thereon. The area of ​​the groove 2' is 0.1mm 2 , with a depth of 0.1mm; the two ends of the groove 2' have a second through hole 11 with an inner diameter of 0.2mm. The elastic material block 3 has a grooved surface and the solid material 4 does not have a grooved surface and is relatively fixed, wherein the first through hole 10 and the second through hole 11 are misplaced and communicated to form a micro flow channel 5, and the inner diameter of the micro flow channel 5 is 0.2 mm. The upper opening of the flow channel 5 is ins...

Embodiment 3

[0075] The first device was made, as shown in Figures 1-3; the method of the present invention was used to immobilize and detect 100 kinds of proteins.

[0076] The device includes 100 grooves. Groove area is 0.06mm 2 , the depth is 0.1mm, and the inner diameter of the microchannel is 0.2mm. The elastic material is silicone, the solid material is aluminum, the tube is stainless steel, and the elastic material membrane for closure is silicone. The chip material is gold-plated glass, and the other structures are the same as in Embodiment 1.

[0077] Under the action of external pressure, the silicon chip is in close contact with the microarray, so that 100 independent sealed cavities are formed between the silicon chip and the groove. Each cavity has two microchannels, one in and one out. Driven by the micro plunger pump, 100 kinds of protein solutions enter the cavity through the micro flow channel to contact and react with the surface of the silicon wafer, thereby immobili...

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Abstract

The invention relates to a method to directly add trace sample and instantly react on the biomolecule chip and device. The method includes: under the action of external pressure, the surfaces of microarray and smooth chips close contact to form a sealed cavity. The bio-ligand molecule which needs fixing enters the cavity through the micro-flow channel and reacts with the chip surfaces, and after reacting, the ligand molecule is discharged through the micro-flow channel. The device includes: elastic material slice carved with grooves, microarray arranged of grooves and micro-flow channel matching the microarray; and elastic material membrane covered on the micro-flow channel, where there is a liquid entrance made on the base slice of microarray or micro-flow channel. After fixed and reacting, the chip surfaces are washed by buffer liquor, to make fixation and reaction of the biomolecule on the surface uniform and consistent, effectively heightening the detecting quality.

Description

technical field [0001] The invention relates to a method for preparing a biomolecular chip and a device thereof, in particular to a method for directly performing micro-sample addition and immediate reaction on a biomolecular chip and a device for realizing the method. Background technique [0002] Biomolecular chips are an integrated and parallel biological detection technology developed in recent years, which can integrate a variety of ligands on a tiny geometric scale, so that multiple indicators of trace samples can be detected at the same time. Due to the high price of biomolecular samples, it is required to use as little as possible, so the biomolecular reagents and samples used in biomolecular chips are required to be miniaturized, which also requires the miniaturization of chip loading and reaction devices. At present, a spotting instrument is mainly used for adding samples to biochips. According to the different spotting methods, it can be divided into two types, o...

Claims

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Application Information

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IPC IPC(8): B01J19/00B01L99/00C12Q1/68G01N33/50G01N33/543G01N33/68
CPCB01L2300/0819B01L3/0293B01L2300/0887B01J19/0046B01L2400/0487
Inventor 王战会靳刚
Owner INST OF MECHANICS - CHINESE ACAD OF SCI
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